TLR3 Antibody

Images

 
Biological Strategies: Western Blot: TLR3 Antibody [NB100-56571] - Myocardial infarction (MI) increased TLR3 expression and its physical association with Trif in mouse myocardium. Heart samples were taken from ...read more
Immunohistochemistry-Frozen: TLR3 Antibody [NB100-56571] - Frozen mouse eye tissue sections stained with TLR3, a retinal pigment epithelium (RPE) marker and DAPI. Top panel: TLR3 (red) colocalized with the RPE (green) ...read more
Flow Cytometry: TLR3 Antibody [NB100-56571] - Analysis using the FITC conjugate of NB100-56571. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype ...read more
Western Blot: TLR3 Antibody [NB100-56571] - Analysis of TLR3 in mouse spleen tissue lysate using NB100-56571 at 2 ug/ml.
Immunohistochemistry: TLR3 Antibody [NB100-56571] - Pig lung poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of IC ventilation. Image from verified customer review.
Flow Cytometry: TLR3 Antibody [NB100-56571] - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.
Genetic Strategies: Knockdown Validated: TLR3 Antibody [NB100-56571] - siRNA was transfected at 50 nM for 48 hrs, and poly(I:C) was added at 100 ug/ml for 4 hrs before cell harvest. Negative control (NC) siRNA ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, RtSpecies Glossary
Applications WB, DB, Flow, Flow-CS, Flow-IC, IHC, IHC-Fr, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TLR3 Antibody Summary

Immunogen
A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
Predicted Species
Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Dot Blot
  • Flow Cytometry 1 ug/10^6 cells
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Knockdown Validated
Application Notes
Flow Cytometry (Cell Surface): see Pawar et al. 2006 for details. Flow Cytometry (Intracellular): 1 ug/10^6 cells. Immunohistochemistry-Frozen: see Pawar et al, 2005 for details. Immunohistochemistry-Paraffin: see Patole et al. 2005 for details. Use in Dot blot reported in scientific literature (PMID: 27248820). Use in Flow-cell surface and Flow-intracellular reported in multiple pieces of scientific literature.
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Control
Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56571 in the following applications:

Publications
Read Publications using
NB100-56571 in the following applications:

Reactivity Notes

Predicted to cross-react with Rat. Porcine data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR3 Antibody

  • CD283 antigen
  • CD283
  • TLR3
  • toll-like receptor 3

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. mTLR3 cDNA codes for a protein with approximate molecular weight of 100 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ca, Rb
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Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-P, IP, KD
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Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
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Species: Hu, Mu, Ca
Applications: WB, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS, Flow-IC
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NBP2-67741
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Species: Hu, Mu, Rb
Applications: WB, Simple Western, DB, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-CS, Flow-IC
NB100-1207
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-75930
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB110-1244
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-56563
Species: Hu, Mu, Rt
Applications: WB, DB, Flow, IHC, IHC-P, Flow-CS, Flow-IC
MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
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Publications for TLR3 Antibody (NB100-56571)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: DB, Flow, Flow-CS, Flow-IC, IHC-Fr, WB.


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(1)
Flow
(1)
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(3)
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(2)
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(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-56571 Applications Species
Nie H, Mei Z et al. Bushen recipe and its disassembled prescriptions inhibit inflammation of liver injury associated with Concanavalin A through Toll like receptor 3/9 signaling pathway. Mol Med Rep Jan 8 2018 [PMID: 29845244] (WB, Mouse) WB Mouse
Gao T, Zhang SP, Wang JF et al. TLR3 contributes to persistent autophagy and heart failure in mice after myocardial infarction J. Cell. Mol. Med. 2017 Sep 25 [PMID: 28945004] (WB, Mouse) WB Mouse
Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination. Oncotarget. Jul 5 2016 [PMID: 27248820] (DB, Human) DB Human
Jiang M, Broering R, Trippler M et al. MicroRNA-155 controls Toll-like receptor 3- and hepatitis C virus-induced immune responses in the liver. J. Viral Hepat. 2014 Feb 1 [PMID: 24383923] (Flow-CS, Mouse)

Details:
BalbC lymphocytes, Fig S1C. Expression of TLR3 was detected using unconjugated anti-TLR3 antibodies and secondary antibodies.
Flow-CS Mouse
Priya R, Dhanwani R, Patro IK et al. Differential regulation of TLR mediated innate immune response of mouse neuronal cells following infection with novel ECSA genotype of Chikungunya virus with and without E1:A226V mutation. Infect Genet Evol 2013 Dec [PMID: 24126361] (WB, Mouse) WB Mouse
Yamashita M, Chattopadhyay S, Fensterl V et al. Epidermal growth factor receptor is essential for Toll-like receptor 3 signaling. Sci Signal. 2012 Jul 17 [PMID: 22810896]
Chen G Y, Hwang S M et al. Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction. J Virol Jan 8 2012 [PMID: 22623765] (Flow, Mouse) Flow Mouse
Kleinman ME, Kaneko H, Cho WG et al. Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3. Mol Ther. 2012 Jan [PMID: 21988875]
Desai MM, Gong B, Chan T et al. Differential, type I interferon-mediated autophagic trafficking of hepatitis C virus proteins in mouse liver. Gastroenterology. 2011 Aug [PMID: 21683701]
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
Show All 16 Publications.

Review for TLR3 Antibody (NB100-56571) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-56571:
Filter by Applications
IHC
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry TLR3 NB100-56571
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4
reviewed by:
Anony- mous
IHC Other 09/18/2014
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Summary

ApplicationImmunohistochemistry
SpeciesOther
Special ApplicationsReview by University of Edinburgh SuRF laboratory. Our team bought TLR 3 antibody NB100-56571 to try and stain porcine lung IHC-P in June/July this year. We have been able to make it work with pH6 retrieval. Please see enclosed picture. This shows a pig lung that has been poisoned through aspiration with gastric juice and organophosphate (dimethoate EC40) after 48 hours of intensive care ventilation.
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Blogs on TLR3.


  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Recent Reviews

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Anony- mous
09/18/2014
Application: IHC
Species: Other

Bioinformatics

Gene Symbol TLR3
Entrez
Uniprot