MDA5 Antibody


Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 more

Product Details

Reactivity Hu, Mu, PmSpecies Glossary
Applications ELISA, Flow, IHC
0.5 mg/ml

Order Details

MDA5 Antibody Summary

Peptide with sequence SNGYSTDENFRYL-C corresponding to N-Terminus according to NP_071451.2.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
MDA5 Overexpression Lysate
Control Peptide
MDA5 Peptide (NB110-1244PEP)
Read Publications using
NB110-1244 in the following applications:

  • 1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27237091).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for MDA5 Antibody

  • AGS7
  • CADM-140 autoantigen
  • Clinically amyopathic dermatomyositis autoantigen 140 kDa
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • EC
  • Helicard
  • Helicase with 2 CARD domains
  • Hlcd
  • IDDM19
  • IFIH1
  • IMD95
  • interferon induced with helicase C domain 1
  • interferon-induced helicase C domain-containing protein 1
  • Interferon-induced with helicase C domain protein 1
  • MDA5
  • MDA-5
  • MDA-5MGC133047
  • MDA5RNA helicase-DEAD box protein 116
  • melanoma differentiation associated protein-5
  • Melanoma differentiation-associated protein 5
  • Murabutide down-regulated protein
  • RH116
  • SGMRT1


Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDA5 Antibody (NB110-1244)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.

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Showing Publications 1 - 8 of 8.
Publications using NB110-1244 Applications Species
Zhu J, Duan G, Wang H et al. TREM-1 activation modulates dsRNA induced antiviral immunity with specific enhancement of MAPk signaling and the RLRs and TLRs on macrophages. Exp. Cell Res. 2016-05-26 [PMID: 27237091] (WB, Mouse) WB Mouse
Chen KR, Chang CH, Huang CY et al. TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. J Biol Chem. 2012-09-14 [PMID: 22833682]
Heutinck KM, Kassies J, Florquin S et al. SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I. Nephrol Dial Transplant. 2012-07-01 [PMID: 22167597] (WB, Human)

MDA5 (IMG-3202). WB: Human renal tubular epithelial (TECs) cells: Fig 2E. TBk1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. MDA5 (IMG-3202).
WB Human
de Koning HD, Rodijk-Olthuis D, van Vlijmen-Willems IM et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol. 2010-11-01 [PMID: 20631729] (IHC-P, Human)

IHC (paraffin): Fig 2 (human epidermis).
IHC-P Human
Sato S, Hoshino K, Satoh T et al. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Arthritis Rheum 2009-07-01 [PMID: 19565506] (WB, Human)

WB (MDA5 transfected COS-7 cells (lane 1) and MDA5 positive human sera (lanes 2-5) that had been IP'D with an autoantibody to MDA5, Fig 2. MDA5 was observed at ~140 kDa. Note: MDA5 is also known as antigen CADM-140. Note: The specificity of the MDA5 (IMG-
WB Human
Liao S, Bao X, Liu T et al. Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling. J Gen Virol. 2008-08-01 [PMID: 18632970] (WB, Human)

Refer to figure 1d (human A549 cells).
WB Human
Bao X, Sinha M, Liu T et al. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology. 2008-04-25 [PMID: 18234263] (WB, Human)

WB (human A549 cell lysates), Fig. 6D.
WB Human
Kang DC, Gopalkrishnan RV, Wu Q et al. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc Natl Acad Sci U S A 2002-01-22 [PMID: 11805321]

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol IFIH1