MDA5 Antibody

Images

 
Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MDA5 Antibody Summary

Immunogen
Peptide with sequence SNGYSTDENFRYL-C corresponding to N-Terminus according to NP_071451.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFIH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB successful usage in transfected cells is reported in scientific literature (PMID: 19565506).
Control
MDA5 Overexpression Lysate
Control Peptide
Publications
Read Publications using
NB110-1244 in the following applications:

  • 1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27237091).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDA5 Antibody

  • CADM-140 autoantigen
  • Clinically amyopathic dermatomyositis autoantigen 140 kDa
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • EC 3.6.4.13
  • helicard
  • Helicase with 2 CARD domains
  • Hlcd
  • IDDM19
  • interferon induced with helicase C domain 1
  • interferon-induced helicase C domain-containing protein 1
  • Interferon-induced with helicase C domain protein 1
  • MDA-5MGC133047
  • MDA5RNA helicase-DEAD box protein 116
  • melanoma differentiation associated protein-5
  • Melanoma differentiation-associated protein 5
  • Murabutide down-regulated protein
  • RH116

Background

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDA5 Antibody (NB110-1244)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-1244 Applications Species
Zhu J, Duan G, Wang H et al. TREM-1 activation modulates dsRNA induced antiviral immunity with specific enhancement of MAPk signaling and the RLRs and TLRs on macrophages. Exp. Cell Res. May 26 2016 [PMID: 27237091] (WB, Mouse) WB Mouse
Chen KR, Chang CH, Huang CY et al. TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. J Biol Chem. 2012 Sep 14 [PMID: 22833682]
Heutinck KM, Kassies J, Florquin S et al. SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I. Nephrol Dial Transplant. 2012 Jul [PMID: 22167597] (WB, Human)

Details:
MDA5 (IMG-3202). WB: Human renal tubular epithelial (TECs) cells: Fig 2E. TBk1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. MDA5 (IMG-3202).
WB Human
de Koning HD, Rodijk-Olthuis D, van Vlijmen-Willems IM et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol. 2010 Nov [PMID: 20631729] (IHC-P, Human)

Details:
IHC (paraffin): Fig 2 (human epidermis).
IHC-P Human
Sato S, Hoshino K, Satoh T et al. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Arthritis Rheum 2009 Jul [PMID: 19565506] (WB, Human)

Details:
WB (MDA5 transfected COS-7 cells (lane 1) and MDA5 positive human sera (lanes 2-5) that had been IP'D with an autoantibody to MDA5, Fig 2. MDA5 was observed at ~140 kDa. Note: MDA5 is also known as antigen CADM-140. Note: The specificity of the MDA5 (IMG-
WB Human
Liao S, Bao X, Liu T et al. Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling. J Gen Virol. 2008 Aug [PMID: 18632970] (WB, Human)

Details:
Refer to figure 1d (human A549 cells).
WB Human
Bao X, Sinha M, Liu T et al. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology. 2008 Apr 25 [PMID: 18234263] (WB, Human)

Details:
WB (human A549 cell lysates), Fig. 6D.
WB Human
Kang DC, Gopalkrishnan RV, Wu Q et al. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc Natl Acad Sci U S A 2002 Jan 22 [PMID: 11805321]

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Product General Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on MDA5.

MDA5 - Part of the RIG-I-like Receptor Family
The innate immune system is responsible for reacting to viral infections through recognition of various viral components. Like toll-like receptor 3 (TLR3), MDA5 recognizes double-stranded (ds) RNA which is a molecular pattern indicative of viral infec...  Read full blog post.

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Bioinformatics

Gene Symbol IFIH1
Entrez
Uniprot