Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)

Images

 
Western Blot: Mouse Spleen Whole Tissue Lysate (Adult Normal) [NB820-59670] - Western blot analysis of Actin (NB600-532) using whole normal mouse spleen lysate [NB820-59670].
SDS-Page: Mouse Spleen Whole Tissue Lysate (Adult Normal) [NB820-59670] - Ponceau S staining of whole normal mouse spleen lysate using NB820-59670.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Concentration
5 mg/ml

Order Details

Mouse Spleen Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Mouse Normal Tissue: Spleen

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol, 0.005% bromophenol blue, pH 6.8]
Concentration
5 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Blood Lymph
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional Array Products

Array NB820-59670

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