Tie-2 Antibody

Images

 
Western Blot: Tie-2 Antibody [NBP1-69753] - Total protein from human Huvec cells and mouse stomach tissue was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry: Tie-2 Antibody [NBP1-69753] - TIE2 antibody was tested in mouse intestines using DAB with hematoxylin counterstain.
Western Blot: Tie-2 Antibody [NBP1-69753] - Detection of TIE2 in Huvec cell lysate
Simple Western: Tie-2 Antibody [NBP1-69753] - Simple Western lane view shows a specific band for Tie-2 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Tie-2 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human TIE2 protein (within residues 250-400). [Swiss-Prot Q02763]
Localization
Membrane, single pass type-1 membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TEK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500
  • Simple Western 1:50
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This TIE2 antibody is useful for Western blot where a band is seen ~150 kDa, and Immunohistochemistry-paraffin embedded sections where membrane, cytoplasmic and nuclear signal is seen in mouse intestines. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publication using
NBP1-69753 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Human and mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Tie-2 Antibody

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

TIE2 (tyrosine-protein kinase receptor TIE-2) is an endothelial-selective RTK that is essential to angiopoietin (ANGPT)/TIE2 system which constitutes transmembrane endothelial tyrosine kinase TIE2 and its circulating ligands (ANGPT-1, -2 and ANGPT-3, -4). Activation/phosphorylation state of TIE2 regulates the baseline endothelial quiescence and its response to pathogens as well as cytokines, and TIE2 phosphorylation is largely controlled by the ratio of agonistic ligand ANGPT-1 and competitive inhibitor ANGPT-2. TIE2 exists as homodimer or heterodimer (with TIE1) and interacts with ANGPT1, ANGPT2, ANGPT4, TEK, TNIP2, SHC1, PTPRB, DOK2, GRB2, GRB7, GRB14, PIK3R1 and PTPN11/SHP2 etc for exerting its diverse biological functions. TIE2 regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, actin cytoskeleton reorganization, and maintenance of vascular quiescence. It exerts anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels, and activates or inhibits angiogenesis, depending on the context (inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts). Targeted deletion of TIE2 or its major agonist ligand, Angpt-1, results in embryonic lethality in mice characterized by defects in blood vessel maturation, lack of recruitment of supporting pericytes and impaired basement membrane formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tie-2 Antibody (NBP1-69753)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-69753AF405
Alexa Fluor 488 Labeled NBP1-69753AF488
Alexa Fluor 647 Labeled NBP1-69753AF647
Alexa Fluor 700 Labeled NBP1-69753AF700
Biotin Labeled NBP1-69753B
DyLight 405 Labeled NBP1-69753V
DyLight 488 Labeled NBP1-69753G
DyLight 550 Labeled NBP1-69753R
DyLight 650 Labeled NBP1-69753C
FITC Labeled NBP1-69753F
HRP Labeled NBP1-69753H

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Bioinformatics

Gene Symbol TEK
Entrez
Uniprot