Tie-2 Antibodies
We offer Tie-2 Antibodies for use in common research applications: Blockade of Receptor-ligand Interaction, ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow Cytometry, Functional, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Sandwich ELISA, Simple Western, Western Blot. Each Tie-2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Tie-2 Antibodies can be used in a variety of model species: Human, Mouse, Rat, Zebrafish. Use the list below to choose the Tie-2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Tie-2 polyclonal antibodies and browse our Tie-2 monoclonal antibody catalog.
Alternate Names for Tie-2 Antibodies
anti-Tie-2 antibody, anti-TEK antibody, anti-angiopoietin-1 receptor antibody, anti-CD202B antibody, anti-CD202b antigen antibody, anti-EC 2.7.10 antibody, anti-EC 2.7.10.1 antibody, anti-hTIE2 antibody, anti-p140 TEK antibody, anti-soluble TIE2 variant 1 antibody, anti-soluble TIE2 variant 2 antibody, anti-TEK tyrosine kinase, endothelial antibody, anti-TIE2CD202b antibody, anti-Tunica interna endothelial cell kinase antibody, anti-Tyrosine-protein kinase receptor TEK antibody, anti-Tyrosine-protein kinase receptor TIE-2 antibody, anti-venous malformations, multiple cutaneous and mucosal antibody, anti-VMCM antibody, anti-VMCM1 antibody
20 Results for "tie-2" in Primary Antibodies
![Pericytes express functional Tie2.(a) Microarray-based expression screening of brain pericytes (BP), placenta pericytes (PP), pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC [HU]). (b) Semi-quantitative PCR of TEK (Tie2), endothelial marker genes (PECAM1, CDH5) and pericyte marker genes (CSPG4, CD248) in HUVEC and BP; house keeping gene: GAPDH. (c) Representative images showing expression of Tie2 in HUVEC and BP. (d) Histogram of membrane-bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry. (e,g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells. (f,h) Quantification of the ratio of phosphorylated Tie2 (pTie2) relative to total Tie2 protein in BP (f) and HUVEC (h) normalized to us control (n=3). (i,k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2-silenced (shTie2 I/shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2. (j,l) Quantification of the ratio of pAKT to total AKT protein expression in BP (j) and HUVEC (l) normalized to unstimulated control (n=3). Representative western blot images are cropped versions and original images can be found in Supplementary Fig. 15. Scale bars: 20 μm (c). Data are shown as mean±s.d. Statistics were performed using Mann–Whitney U test (f,h) and one-way ANOVA (j,l). *P<0.05, **P<0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28719590), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af762_mouse-rat-tie-2-affinity-purified-polyclonal-ab-8220249505990.jpg)
![Pericytes express functional Tie2.(a) Microarray-based expression screening of brain pericytes (BP), placenta pericytes (PP), pancreas pericytes (PA), lung pericytes (LP), muscle pericytes (MP) and human umbilical vein endothelial cells (HUVEC [HU]). (b) Semi-quantitative PCR of TEK (Tie2), endothelial marker genes (PECAM1, CDH5) and pericyte marker genes (CSPG4, CD248) in HUVEC and BP; house keeping gene: GAPDH. (c) Representative images showing expression of Tie2 in HUVEC and BP. (d) Histogram of membrane-bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry. (e,g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells. (f,h) Quantification of the ratio of phosphorylated Tie2 (pTie2) relative to total Tie2 protein in BP (f) and HUVEC (h) normalized to us control (n=3). (i,k) Western blot analysis of AKT phosphorylation (Ser473) in control (shCtr) and Tie2-silenced (shTie2 I/shTie2 II) BP (i) and HUVEC (k) upon stimulation with rhAng1 or rhAng2. (j,l) Quantification of the ratio of pAKT to total AKT protein expression in BP (j) and HUVEC (l) normalized to unstimulated control (n=3). Representative western blot images are cropped versions and original images can be found in Supplementary Fig. 15. Scale bars: 20 μm (c). Data are shown as mean±s.d. Statistics were performed using Mann–Whitney U test (f,h) and one-way ANOVA (j,l). *P<0.05, **P<0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28719590), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af762_mouse-rat-tie-2-affinity-purified-polyclonal-ab-4420241543319.jpg)
![Western Blot: Tie-2 Antibody [NBP1-69753] - Total protein from human Huvec cells and mouse stomach tissue was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TIE2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Tie-2-Antibody-Western-Blot-NBP1-69753-img0006.jpg "Western Blot: Tie-2 Antibody [NBP1-69753] - Total protein from human Huvec cells and mouse stomach tissue was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TIE2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Tie-2 Antibody [NBP1-69753] - Analysis of a FFPE tissue section of mouse stomach using 1:200 dilution of Tie-2 antibody (NBP1-69753). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Tie-2-Antibody-Immunohistochemistry-Paraffin-NBP1-69753-img0007.jpg)
![Western Blot: Tie-2 Antibody [NBP1-69753] - Detection of TIE2 in Huvec cell lysate](http://images.novusbio.com/fullsize/Tie-2-Antibody-Western-Blot-NBP1-69753-img0003.jpg)
![Simple Western: Tie-2 Antibody [NBP1-69753] - Simple Western lane view shows a specific band for Tie-2 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Tie-2-Antibody-Simple-Western-NBP1-69753-img0005.jpg)
![Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).](http://images.novusbio.com/fullsize/Tie-2-Antibody-tek9-Flow-Cytometry-NBP1-18614-img0014.jpg "Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).")
![Sandwich ELISA: Tie-2 Antibody (tek9) [NBP1-18614] - Quantification of soluble and cellular Tie-2 by sandwich ELISA. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTie-2 or Tie-2. For capturing anti-human Tie-2 Cl.16 NBP1-18616 was used, for the detection of a mixture of biotinylated anti-human Tie-2 Cl.2 NBP1-18612 and Cl.9 NBP1-18614.](http://images.novusbio.com/fullsize/Tie-2-Antibody-tek9-Sandwich-ELISA-NBP1-18614-img0015.jpg)
![Flow Cytometry: Tie-2 Antibody (B-B48) [NBP3-14575] - A typical staining pattern with the B-B48 monoclonal antibody of Eahy 926 cell line.](http://images.novusbio.com/fullsize/Tie-2-Antibody-B-B48-Flow-Cytometry-NBP3-14575-img0001.jpg "Flow Cytometry: Tie-2 Antibody (B-B48) [NBP3-14575] - A typical staining pattern with the B-B48 monoclonal antibody of Eahy 926 cell line.")