Tie-2 ELISA Kits

BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_lightbox"> BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." alt="Human Tie-2 transfected Baf/3 cells (Baf/3-hTie-2) were treated with 600 ng/mL recombinant human Ang-1 for seven minutes to induce tyrosine phosphorylation of Tie-2. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-Tie-2 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGlo^TM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_thumb" />

BAF313). Human Phospho-Tie-2 can be detected in this ELISA by using approximately 4 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox"> BAF313). Human Phospho-Tie-2 can be detected in this ELISA by using approximately 4 times less lysate than is needed for a conventional IP-Western blot." class="big_thumb" />

313-TI), human Tie-1 (Catalog #619-TI), human VEGF R2 (Catalog #357-KD) or human VEGF R3 (Catalog #321-FL) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant Tie-2. " class="big_lightbox"> 313-TI), human Tie-1 (Catalog #619-TI), human VEGF R2 (Catalog #357-KD) or human VEGF R3 (Catalog #321-FL) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant Tie-2. " class="big_thumb" />