Tie-1 Antibody [Unconjugated]


HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more
Tie‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready

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Tie-1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Tie-1
Accession # P35590
Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Tie-1 is observed and less than 1% cross-reactivity with recombinant human Tie‑2 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF619 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-1 Antibody [Unconjugated]

  • EC 2.7.10
  • EC
  • JTK14soluble TIE1 variant 3
  • receptor tyrosine kinase
  • soluble TIE1 variant 1
  • soluble TIE1 variant 2
  • soluble TIE1 variant 5
  • Tie1
  • Tie-1
  • TIEsoluble TIE1 variant 4
  • tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1


Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159
  2. Sato, T.N. et al. (1995) Nature 376:70


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tie-1 Antibody (AF619)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 10 of 10.
Publications using AF619 Applications Species
YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (Western Blot, Mouse) Western Blot Mouse
LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions PloS Pathogens, 2022-01-31;18(1):e1010280. 2022-01-31 [PMID: 35100311] (Western Blot, Human) Western Blot Human
ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils Sulfated glycans engage the Ang-Tie pathway to regulate vascular development Nat Chem Biol, 2020-10-05;0(0):. 2020-10-05 [PMID: 33020664] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
VM Leppänen, P Saharinen, K Alitalo Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):. 2017-04-10 [PMID: 28396439] (Western Blot, Human) Western Blot Human
N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling Circ. Res, 2017-02-08;0(0):. 2017-02-08 [PMID: 28179430] (IHC, Mouse) IHC Mouse
Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015-01-30;6(0):5962. 2015-01-30 [PMID: 25635707] (IHC, Human) IHC Human
Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2. J. Biol. Chem., 2007-08-29;282(42):30509-17. 2007-08-29 [PMID: 17728252] (Western Blot, Human) Western Blot Human
Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells. Cardiovasc. Res., 2006-08-07;72(3):394-402. 2006-08-07 [PMID: 17054925] (Western Blot, Human) Western Blot Human
Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC Expansion of human SCID-repopulating cells under hypoxic conditions. J. Clin. Invest., 2003-07-01;112(1):126-35. 2003-07-01 [PMID: 12840067] (Flow Cytometry, Human) Flow Cytometry Human
Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2. J. Biol. Chem., 2000-12-15;275(50):39741-6. 2000-12-15 [PMID: 10995770] (Immunoprecipitation, Human) Immunoprecipitation Human

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Gene Symbol TIE1