Tie-1 Antibody [Unconjugated]

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Tie‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was ...read more
HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Tie-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie-1
Ala22-Gln760
Accession # P35590
Specificity
Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Tie-1 is observed and less than 1% cross-reactivity with recombinant human Tie‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIE1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Publications
Read Publications using
AF619 in the following applications:

  • 1 publication
  • IF
    1 publication
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-1 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • JTK14soluble TIE1 variant 3
  • receptor tyrosine kinase
  • soluble TIE1 variant 1
  • soluble TIE1 variant 2
  • soluble TIE1 variant 5
  • Tie1
  • Tie-1
  • TIEsoluble TIE1 variant 4
  • tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. Ligands which bind and activate Tie-1 have not been identified. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159
  2. Sato, T.N. et al. (1995) Nature 376:70

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tie-1 Antibody (AF619)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow, IF, IHC, IP, WB.


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Flow
(1)
IF
(1)
IHC
(1)
IP
(1)
WB
(3)
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF619 Applications Species
VM Leppänen, P Saharinen, K Alitalo Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28396439] (WB,, Human) WB Human
N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling Circ. Res, 2017;0(0):. 2017 [PMID: 28179430] (IHC,, Mouse) IHC Mouse
Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P Endothelial destabilization by angiopoietin-2 via integrin beta1 activation. Nat Commun, 2015;6(0):5962. 2015 [PMID: 25635707] (IF,, Human) IF Human
Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2. J. Biol. Chem., 2007;282(42):30509-17. 2007 [PMID: 17728252] (WB,, Human) WB Human
Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells. Cardiovasc. Res., 2006;72(3):394-402. 2006 [PMID: 17054925] (WB,, Human) WB Human
Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC Expansion of human SCID-repopulating cells under hypoxic conditions. J. Clin. Invest., 2003;112(1):126-35. 2003 [PMID: 12840067] (Flow,, Human) Flow Human
Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2. J. Biol. Chem., 2000;275(50):39741-6. 2000 [PMID: 10995770] (IP,, Human) IP Human

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Bioinformatics

Gene Symbol TIE1
Entrez
Uniprot