Home » Tie-2 Products

We're moving to rndsystems.com. Come with us!

After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.

Register on R&D Systems

Tie-2 Products

Antibodies
Tie-2 Antibody [Unconjugated] ...
Tie-2 Antibody [Unconjugated]
AF313
HAF017). A specific band was detected for Tie-2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank"> Western Blot Tie-2 Antibody [Unconjugated]
CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank"> Immunohistochemistry Tie-2 Antibody [Unconjugated]
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank"> Simple Western Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, B/N
Host: Goat Polyclonal
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF313U-100UG
Alexa Fluor 405 AF313V-100UG
Alexa Fluor 488 AF313G-100UG
Alexa Fluor 532 AF313X-100UG
Alexa Fluor 594 AF313T-100UG
Alexa Fluor 647 AF313R-100UG
Alexa Fluor 700 AF313N-100UG
Alexa Fluor 750 AF313S-100UG
Alexa Fluor Plus 405 AF313AFP405-100UG
Alexa Fluor Plus 488 AF313AFP488-100UG
Alexa Fluor Plus 555 AF313AFP555-100UG
Alexa Fluor Plus 594 AF313AFP594-100UG
Alexa Fluor Plus 647 AF313AFP647-100UG
Alexa Fluor Plus 680 AF313AFP680-100UG
Allophycocyanin AF313A
Biotin BAF313
CoraFluor 1 AF313CL1
DyLight 350 AF313D
DyLight 405 AF313E
DyLight 488 AF313K
DyLight 550 AF313L
DyLight 594 AF313M
DyLight 650 AF313W
DyLight 680 AF313Y
DyLight 755 AF313Z
FITC AF313F
HRP AF313H
Janelia Fluor 525 AF313JF525
Janelia Fluor 549 AF313I
Janelia Fluor 646 AF313J
Janelia Fluor 669 AF313JF669
PE AF313P
PerCP AF313C
mFluor Violet 450 SE AF313MFV450
mFluor Violet 500 SE AF313MFV500
mFluor Violet 610 SE AF313MFV610
Formulation Catalog # Availability Price  
Carrier Free AF313-SP
Tie-2 Antibody [Unconjugated] ...
Tie-2 Antibody [Unconjugated]
AF762
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank"> Immunohistochemistry Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Western Blot Tie-2 Antibody [Unconjugated]
Species: Mu, Rt
Applications: WB, IHC
Host: Goat Polyclonal
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF762U-100UG
Alexa Fluor 405 AF762V-100UG
Alexa Fluor 488 AF762G-100UG
Alexa Fluor 532 AF762X-100UG
Alexa Fluor 594 AF762T-100UG
Alexa Fluor 647 AF762R-100UG
Alexa Fluor 700 AF762N-100UG
Alexa Fluor 750 AF762S-100UG
Alexa Fluor Plus 405 AF762AFP405-100UG
Alexa Fluor Plus 488 AF762AFP488-100UG
Alexa Fluor Plus 555 AF762AFP555-100UG
Alexa Fluor Plus 594 AF762AFP594-100UG
Alexa Fluor Plus 647 AF762AFP647-100UG
Alexa Fluor Plus 680 AF762AFP680-100UG
Allophycocyanin AF762A
Biotin BAF762
CoraFluor 1 AF762CL1
DyLight 350 AF762D
DyLight 405 AF762E
DyLight 488 AF762K
DyLight 550 AF762L
DyLight 594 AF762M
DyLight 650 AF762W
DyLight 680 AF762Y
DyLight 755 AF762Z
FITC AF762F
HRP AF762H
Janelia Fluor 525 AF762JF525
Janelia Fluor 549 AF762I
Janelia Fluor 646 AF762J
Janelia Fluor 669 AF762JF669
PE AF762P
PerCP AF762C
mFluor Violet 450 SE AF762MFV450
mFluor Violet 500 SE AF762MFV500
mFluor Violet 610 SE AF762MFV610
Formulation Catalog # Availability Price  
Carrier Free AF762-SP
Tie-2 [p Tyr992] Antibody [Un ...
Tie-2 [p Tyr992] Antibody [Unconju...
AF2720
923-AN) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Tie-2 (Y992) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank"> Western Blot Tie-2 [p Tyr992] Antibody [Unconjugated]
CTS013). Tissue was stained using the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank"> Immunohistochemistry Tie-2 [p Tyr992] Antibody [Unconjugated]
AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox" target="_blank"> Intracellular Staining by Flow Cytometry Tie-2 [p Tyr992] Antibody [Unconjugated]
Western Blot Tie-2 [p Tyr992] Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB, IHC, CyTOF-ready, Flow
Host: Rabbit Polyclonal
Formulation Catalog # Availability Price  
Carrier Free AF2720-SP
Read More
Read More
Read More
See All Tie-2 Products
We have 37 Tie-2 Antibodies
Antibody Pairs
Tie-2 Antibody Pair [HRP]
Tie-2 Antibody Pair [HRP]
NBP2-79518
Sandwich ELISA Tie-2 Antibody Pair [HRP]
Species: Hu
Applications: ELISA
Host: Rabbit
Conjugate Catalog # Availability Size Price
HRP NBP2-79518-5Plates
HRP NBP2-79518-15Plates
Read More
See All Tie-2 Products
We have 1 Tie-2 Antibody Pair
ELISA Kits
Human Tie-2 DuoSet ELISA, 15 ...
Human Tie-2 DuoSet ELISA, 15 Plate
DY5159
N/A Tie-2 [Biotin]
Species: Hu
Applications: ELISA
Human Phospho-Tie-2 DuoSet IC ...
Human Phospho-Tie-2 DuoSet IC ELIS...
DYC2720-2
BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004)." class="big_lightbox" target="_blank"> ELISA Tie-2 [Biotin]
BAF313). Human Phospho-Tie-2 can be detected in this ELISA by using approximately 4 times less lysate than is needed for a conventional IP-Western blot." class="big_lightbox" target="_blank"> ELISA Tie-2 [Biotin]
313-TI), human Tie-1 (Catalog #619-TI), human VEGF R2 (Catalog #357-KD) or human VEGF R3 (Catalog #321-FL) were added to 50 μg lysate and analyzed using this ELISA. Competition was observed only with recombinant Tie-2. " class="big_lightbox" target="_blank"> ELISA Tie-2 [Biotin]
Species: Hu
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC2720E
Biotin DYC2720-5
Mouse Phospho-Tie-2 DuoSet IC ...
Mouse Phospho-Tie-2 DuoSet IC ELIS...
DYC2816-2
Species: Mu
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC2816-5
Read More
Read More
Read More
See All Tie-2 Products
We have 7 Tie-2 ELISA Kits
Lysates
Tie-2 Overexpression Lysate
Tie-2 Overexpression Lysate
NBL1-16808
Western Blot Tie-2 Overexpression Lysate
Species: Hu
Applications: WB
Read More
See All Tie-2 Products
We have 1 Tie-2 Lysate
Proteins
Recombinant Human Tie-2 Fc Ch ...
Recombinant Human Tie-2 Fc Chimera...
313-TI
623-AN) binds with an ED50 of 0.5-4 ng/mL." class="big_lightbox" target="_blank"> Binding Activity Tie-2 [Unconjugated]
Species: Hu
Applications: Bioactivity
Formulation Catalog # Availability Price  
Carrier Free 313-TI-100
Recombinant Mouse Tie-2 Fc Ch ...
Recombinant Mouse Tie-2 Fc Chimera...
762-T2
Species: Mu
Applications: Binding Activity
Formulation Catalog # Availability Price  
Carrier Free 762-T2-100
Recombinant Cynomolgus Monkey ...
Recombinant Cynomolgus Monkey Tie-...
10341-T2
623-AN) binds with an ED50 of 1.5-12 ng/mL." class="big_lightbox" target="_blank"> Binding Activity Tie-2 [Unconjugated]
SDS-Page Tie-2 [Unconjugated]
Species: Pm-Cm
Applications: Bioactivity
Formulation Catalog # Availability Price  
Carrier Free 10341-T2-100
Read More
Read More
Read More
See All Tie-2 Products
We have 14 Tie-2 Proteins

Description

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.

Bioinformatics

Entrez
21687
Mouse
7010
Human
Uniprot
AAH35514
Human
ABW03349.1
Human
NM_000459
Human
NP_000450
Human
Q02763
Human
Q02858
Mouse
Product By Gene ID 7010
Alternate Names
  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202B
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TIE-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Research Areas for Tie-2

Find related products by research area and learn more about each of the different research areas below.

Angiogenesis
Cancer
Phospho-Specific
Protein Kinase

Related Tie-2 Blog Posts

Check out the latest blog posts on Tie-2.
Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...    Read more.
Read more Tie-2 related blogs.