Human Angiogenin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Angiogenin Quantikine ELISA Kit Summary

• Background: The Quantikine Human Angiogenin Immunoassay is a 2.5 hour solid phase ELISA designed to measure Angiogenin in cell culture supernates, serum, and plasma. It contains recombinant human Angiogenin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate both natural and recombinant human Angiogenin.
• Specificity: Natural and recombinant human Angiogenin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ANG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Angiogenin Quantikine ELISA Kit

• ALS9
• ANG
• Angiogenin
• angiogenin, ribonuclease, RNase A family, 5
• EC 3.1.27
• EC 3.1.27.-
• epididymis luminal protein 168
• HEL168
• MGC22466
• MGC71966
• Ribonuclease 5
• RNase 5
• RNASE5RNASE4

Background

Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Angiogenin (DAN00)(24)

Publications using DAN00

• Neubauer-Geryk, J;My?liwiec, M;Zorena, K;Bieniaszewski, L; Parameters of Micro- and Macrocirculation in Young Uncomplicated Type 1 Diabetic Patients-The Role of Metabolic Memory International journal of molecular sciences 2025-10-18 [PMID: 41155446] (Human)
• GM Aldawsari, S Sabico, AA Alamro, A Alenad, K Wani, AM Alnaami, MNK Khattak, MS Masoud, NM Al-Daghri, MS Alokail Angiogenin Levels and Their Association with Cardiometabolic Indices Following Vitamin D Status Correction in Saudi Adults Biology, 2022-02-11;11(2):. 2022-02-11 [PMID: 35205153] (Human)
• D Weng, T Han, J Dong, M Zhang, Y Mi, Y He, X Li, X Zhu Angiogenin and MMP-2 as potential biomarkers in the differential diagnosis of gestational trophoblastic diseases Medicine, 2022-02-04;101(5):e28768. 2022-02-04 [PMID: 35119039] (Human)
• T Fukushi, T Yamamoto, M Yoshida, E Fujikura, M Miyazaki, M Nakayama Enhanced neutrophil apoptosis accompanying myeloperoxidase release during hemodialysis Scientific Reports, 2020-12-10;10(1):21747. 2020-12-10 [PMID: 33303892] (Human)
• HM Choi, YE Kwon, S Kim, DJ Oh Changes in FGF-23, Neutrophil/Platelet Activation Markers, and Angiogenin in Advanced Chronic Kidney Disease and Their Effect on Arterial Stiffness Kidney Blood Press. Res., 2019-09-25;0(0):1-13. 2019-09-25 [PMID: 31553973] (Human)
• M Crivello, SL O'Riordan, I Woods, S Cannon, L Halang, KS Coughlan, MC Hogg, SA Lewandowsk, JHM Prehn Pleiotropic activity of systemically delivered angiogenin in the SOD1G93Amouse model Neuropharmacology, 2018-02-25;133(0):503-511. 2018-02-25 [PMID: 29486168] (Human)
• B Fohlen, Q Tavernier, TM Huynh, C Caradeuc, D Le Corre, G Bertho, B Cholley, N Pallet Real-Time and Non-invasive Monitoring of the Activation of the IRE1?-XBP1 Pathway in Individuals with Hemodynamic Impairment EBioMedicine, 2017-12-20;0(0):. 2017-12-20 [PMID: 29276149] (Human)
• RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 2017-07-07;8(37):62081-62098. 2017-07-07 [PMID: 28977928] (Human)
• Martin R Wilkins Plasma Metabolomics Implicate Modified Transfer RNAs and Altered Bioenergetics in the Outcome of Pulmonary Arterial Hypertension Circulation, 2016-11-21;0(0):. 2016-11-21 [PMID: 27881557] (Human)
• Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors. PLoS ONE, 2015-05-07;10(5):e0126190. 2015-05-07 [PMID: 25951297] (Human)
• Santhekadur PK, Das SK, Gredler R, Chen D, Srivastava J, Robertson C, Baldwin AS, Fisher PB, Sarkar D Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221. J. Biol. Chem., 2012-03-06;287(17):13952-8. 2012-03-06 [PMID: 22396537] (Human)
• Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer. Anticancer Res., 2011-08-01;31(8):2651-5. 2011-08-01 [PMID: 21778318] (Human)
• Aronis KN, Diakopoulos KN, Fiorenza CG Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans. Diabetologia, 2011-06-10;54(9):2358-67. 2011-06-10 [PMID: 21660636] (Human)
• Idriss NK, Lip GY, Balakrishnan B Plasma haemoxygenase-1 in coronary artery disease. A comparison with angiogenin, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and vascular endothelial growth factor. Thromb. Haemost., 2010-09-13;104(5):1029-37. 2010-09-13 [PMID: 20838751] (Human)
• Siebert J, Reiwer-Gostomska M, Mysliwska J Glycemic control influences serum angiogenin concentrations in patients with type 2 diabetes. Diabetes Care, 2010-05-18;33(8):1829-30. 2010-05-18 [PMID: 20484129] (Human)
• Bourlev V, Iljasova N, Adamyan L, Larsson A, Olovsson M Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis. Fertil. Steril., 2009-03-25;94(1):52-7. 2009-03-25 [PMID: 19324337] (Human)
• Tello-Montoliu A, Marin F, Patel J, Roldan V, Mainar L, Vicente V, Sogorb F, Lip GY Plasma angiogenin levels in acute coronary syndromes: implications for prognosis. Eur. Heart J., 2007-11-02;28(24):3006-11. 2007-11-02 [PMID: 17981827] (Human)
• Siebert J, Reiwer-Gostomska M, Babinska Z, Mysliwska J, Mysliwski A, Skopinska-Rozewska E, Sommer E, Skopinski P Low serum angiogenin concentrations in patients with type 2 diabetes. Diabetes Care, 2007-09-18;30(12):3086-7. 2007-09-18 [PMID: 17878246] (Human)
• Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Exp. Hematol., 2007-07-01;35(7):1119-31. 2007-07-01 [PMID: 17588481] (Human)
• Anagnostopoulos A, Eleftherakis-Papaiakovou V, Kastritis E, Tsionos K, Bamias A, Meletis J, Dimopoulos MA, Terpos E Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity. Br. J. Haematol., 2007-06-01;137(6):560-8. 2007-06-01 [PMID: 17451406] (Human)
• Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease. Leuk. Lymphoma, 2006-06-01;47(6):1034-40. 2006-06-01 [PMID: 16840194] (Human)
• Abdel-Rahman AM, el-Sahrigy SA, Bakr SI A comparative study of two angiogenic factors: vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack. Chest, 2006-02-01;129(2):266-71. 2006-02-01 [PMID: 16478840] (Human)
• Eissa S, Kenawy G, Swellam M, El-Fadle AA, Abd El-Aal AA, El-Ahmady O Comparison of cytokeratin 20 RNA and angiogenin in voided urine samples as diagnostic tools for bladder carcinoma. Clin. Biochem., 2004-09-01;37(9):803-10. 2004-09-01 [PMID: 15329320] (Human)
• Suzumori N, Zhao XX, Suzumori K Elevated angiogenin levels in the peritoneal fluid of women with endometriosis correlate with the extent of the disorder. Fertil. Steril., 2004-07-01;82(1):93-6. 2004-07-01 [PMID: 15236995] (Human)

FAQs for Angiogenin (DAN00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANG