Goat IgG Isotype Control [Unconjugated]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (AB-108-C)(211)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig, Hamster, Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 28 applications: Bioassay, Bioassay - Ctrl, Blocking, ChIP, ChIP Assay, Co-immunoprecipitation, Ctrl, Flow, Flow Ctrl, Flow control, Functional Assay, ICC, ICC Ctrl, IF, IHC, IHC Frozen, IHC - Paraffin embedded, IHC Ctrl, IHC OCT-embedded, IHC Paraffin-embedded, IP, In Vivo, In vivo, In vivo control, Isotype control, Neut, Neut Ctrl, contorl.


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Bioassay
(6)
Bioassay - Ctrl
(1)
Blocking
(1)
ChIP
(2)
ChIP Assay
(1)
Co-immunoprecipitation
(1)
Ctrl
(103)
Flow
(8)
Flow Ctrl
(2)
Flow control
(1)
Functional Assay
(3)
ICC
(1)
ICC Ctrl
(1)
IF
(1)
IHC
(5)
IHC Frozen
(1)
IHC - Paraffin embedded
(2)
IHC Ctrl
(1)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(2)
IP
(4)
In Vivo
(1)
In vivo
(12)
In vivo control
(3)
Isotype control
(9)
Neut
(21)
Neut Ctrl
(12)
contorl
(1)
All Applications
Filter By Species
Human
(86)
Mouse
(96)
Rat
(18)
Chicken
(2)
Guinea Pig
(1)
Hamster
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 211. Show All 211 Publications.
Publications using AB-108-C Applications Species
F Ferrandino, G Bernardini, G Tsaouli, P Grazioli, AF Campese, C Noce, A Ciuffetta, A Vacca, ZM Besharat, D Bellavia, I Screpanti, MP Felli Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagation Oncogene, 2018;0(0):. 2018 [PMID: 30038265] (Flow,, Mouse) Flow Mouse
F Wang, B Li, Y Wei, Y Zhao, L Wang, P Zhang, J Yang, W He, H Chen, Z Jiao, Y Li Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression Oncogenesis, 2018;7(5):41. 2018 [PMID: 29799520] (Functional Assay,, Human) Functional Assay Human
Q Guo, T Grimmig, G Gonzalez, A Giobbie-Hu, G Berg, N Carr, BJ Wilson, P Banerjee, J Ma, JS Gold, B Nandi, Q Huang, AM Waaga-Gass, CG Lian, GF Murphy, MH Frank, M Gasser, NY Frank ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29789423] (Flow,, Xenograft) Flow Xenograft
X Qu, X Dang, W Wang, Y Li, D Xu, D Shang, Y Chang Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease Mediators Inflamm., 2018;2018(0):7501851. 2018 [PMID: 29725270] (Isotype control, ) Isotype control
S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain J. Neurosci., 2018;0(0):. 2018 [PMID: 29712778] (Isotype control,, Rat) Isotype control Rat
A Bertero, S Brown, P Madrigal, A Osnato, D Ortmann, L Yiangou, J Kadiwala, NC Hubner, IR de Los Moz, C Sadée, AS Lenaerts, S Nakanoh, R Grandy, E Farnell, J Ule, HG Stunnenber, S Mendjan, L Vallier The SMAD2/3 interactome reveals that TGF? controls m6A mRNA methylation in pluripotency Nature, 2018;555(7695):256-259. 2018 [PMID: 29489750] (IP,, Human) IP Human
G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis Immunol. Cell Biol., 2018;0(0):. 2018 [PMID: 29359350] (Neut,, Human) Neut Human
I Gregersen, Ø Sandanger, ET Askevold, EL Sagen, K Yang, S Holm, TM Pedersen, M Skjelland, K Krohg-Søre, TV Hansen, TB Dahl, K Otterdal, T Espevik, P Aukrust, A Yndestad, B Halvorsen Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation PLoS ONE, 2017;12(11):e0188387. 2017 [PMID: 29176764] (Neut Ctrl,, Human) Neut Ctrl Human
AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling J. Immunol., 2017;0(0):. 2017 [PMID: 29070670] (Ctrl,, Human) Ctrl Human
Y Shlyakhtin, V Pavet, H Gronemeyer Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells Cell Death Dis, 2017;8(8):e3025. 2017 [PMID: 29048428] (IP,, Human) IP Human
Show All 211 Publications.

Review for IgG Isotype Control (AB-108-C) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using AB-108-C:
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG AB-108-C
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reviewed by:
Anonymous
ICC/IF 04/07/2017
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
04/07/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol IGHG1