Goat IgG Isotype Control [Unconjugated]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Dilutions
  • Control
Reviewed Applications
Read 4 Reviews rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (AB-108-C)(312)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Bovine, Canine, Chicken, Ferret, Guinea Pig, Hamster, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Xenograft.

We have publications tested in 29 applications: Binding Assay, Binding Control, Bioassay, Blocking, Cell Culture, ChIP, ChIP Control, Co-Immunoprecipitation, Control, Flow Cytometry, Flow Cytometry Control, Functional Assay, ICC, ICC Control, IHC, IHC Control, IHC-F, IHC-Fr, IHC-P, Immunoprecipitation, Immunoprecipitation (ChIP), In Vivo, Isotype Control, Isotype control, Neutralization, Neutralization Control, Tissue Culture, Western Blot, control for neutralization.


Filter By Application
Binding Assay
(1)
Binding Control
(1)
Bioassay
(10)
Blocking
(1)
Cell Culture
(4)
ChIP
(4)
ChIP Control
(1)
Co-Immunoprecipitation
(2)
Control
(117)
Flow Cytometry
(13)
Flow Cytometry Control
(3)
Functional Assay
(4)
ICC
(4)
ICC Control
(1)
IHC
(12)
IHC Control
(4)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(5)
Immunoprecipitation
(7)
Immunoprecipitation (ChIP)
(1)
In Vivo
(8)
Isotype Control
(24)
Isotype control
(1)
Neutralization
(49)
Neutralization Control
(20)
Tissue Culture
(1)
Western Blot
(4)
control for neutralization
(1)
All Applications
Filter By Species
Human
(133)
Mouse
(139)
Rat
(21)
Bacteria - Streptococcus pyogenes
(1)
Bovine
(1)
Canine
(1)
Chicken
(2)
Ferret
(1)
Guinea Pig
(1)
Hamster
(2)
Porcine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rabbit
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 312. Show All 312 Publications.
Publications using AB-108-C Applications Species
Y Menuchin-L, A Schreiber, A Lecanda, A Mecate-Zam, L Brunotte, OE Psathaki, S Ludwig, T Rauen, HR Schöler SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35334213] (Neutralization, Human) Neutralization Human
K Molnár, B Nógrádi, R Kristóf, Á Mészáros, K Pajer, L Siklós, A Nógrádi, I Wilhelm, IA Krizbai Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury Journal of Neuroinflammation, 2022;19(1):68. 2022 [PMID: 35305649] (Western Blot, Mouse) Western Blot Mouse
K Kos, MA Aslam, R van de Ven, MD Wellenstei, W Pieters, A van Weverw, DEM Duits, K van Pul, CS Hau, K Vrijland, D Kaldenbach, EAM Raeven, SA Quezada, R Beyaert, H Jacobs, TD de Gruijl, KE de Visser Tumor-educated Tregs drive organ-specific metastasis in breast cancer by impairing NK cells in the lymph node niche Cell Reports, 2022;38(9):110447. 2022 [PMID: 35235800] (Neutralization Control, Mouse) Neutralization Control Mouse
A Ichikawa, S Neo, R Nukui, Y Yamada, S Nitta, H Iwaki, Y Yanagi, K Nakayama, S Sato, S Tateishi, M Hisasue Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cells Regenerative Therapy, 2022;19(0):1-8. 2022 [PMID: 35024388] (IHC Control, Canine) IHC Control Canine
IC Kreft, RRA Winiarczyk, FJ Tanis, C van der Zw, KS Schmitz, AJ Hoogendijk, RL de Swart, A Moscona, M Porotto, DCF Salvatori, RD de Vries, MPM de Maat, M van den Bi, BJM van Vlijme, Dutch Covi Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in the ferret model Thrombosis Research, 2021;210(0):6-11. 2021 [PMID: 34954402] (IHC Control, Ferret) IHC Control Ferret
J Wang, G Yang, X Wang, Z Wen, L Shuai, J Luo, C Wang, Z Sun, R Liu, J Ge, X He, R Hua, X Wang, X Yang, W Chen, G Zhong, Z Bu SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells Cell Discovery, 2021;7(1):119. 2021 [PMID: 34903715] (Neutralization, Human) Neutralization Human
P Li, Q Li, N Biswas, H Xin, T Diemer, L Liu, L Perez Guti, G Paternostr, C Piermarocc, S Domanskyi, RK Wang, N Ferrara LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy Embo Molecular Medicine, 2021;0(0):e14511. 2021 [PMID: 34779136] (Neutralization Control, Bovine) Neutralization Control Bovine
D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021;0(0):e12924. 2021 [PMID: 34762341] (Neutralization Control, Human) Neutralization Control Human
E Gomez-Esco, J Burlaud-Ga, C Visdeloup, ARE Silva, P Coutant, P Roingeard, E Beaumont Incorporation of apolipoprotein E into HBV-HCV subviral envelope particles to improve the hepatitis vaccine strategy Scientific Reports, 2021;11(1):21856. 2021 [PMID: 34750487] (Co-Immunoprecipitation, Hamster) Co-Immunoprecipitation Hamster
YC Chen, CY Chien, CC Hsu, CH Lee, YT Chou, SG Shiah, SY Liu, CY Yen, AC Hsieh, M Wabitsch, YS Shieh Obesity-associated leptin promotes chemoresistance in colorectal cancer through YAP-dependent AXL upregulation American journal of cancer research, 2021;11(9):4220-4240. 2021 [PMID: 34659884] (In Vivo, Mouse) In Vivo Mouse
Show All 312 Publications.

Reviews for IgG Isotype Control (AB-108-C) (4) 54

Average Rating: 5
(Based on 4 reviews)

Reviews using AB-108-C:
Filter by Applications
ICC/IF
(2)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ICC/IF 05/07/2019
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lotes4118071
  5
reviewed by:
Verified Customer
05/07/2019
View

Summary

ApplicationSerum
Lotes4118071
Immunocytochemistry/Immunofluorescence IgG AB-108-C
Enlarge
5
reviewed by:
Verified Customer
ICC/IF 04/07/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.
Serum IgG AB-108-C
Enlarge
5
reviewed by:
Verified Customer
04/07/2017
View

Summary

ApplicationSerum
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

 
Verified Customer
05/07/2019
Application: ICC/IF
Species:

 
Verified Customer
05/07/2019
Application:
Species:

Verified Customer
04/07/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol IGHG1