Goat IgG Isotype Control [Unconjugated]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Reviewed Applications
Read 4 Reviews rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (AB-108-C)(244)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Chicken, Guinea Pig, Hamster, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 26 applications: Binding Control, Bioassay, Blocking, Cell Culture, ChIP, Co-Immunoprecipitation, Control, Flow Cytometry, Flow Cytometry Control, Functional Assay, ICC, ICC Control, IHC, IHC Control, IHC-F, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Isotype Control, Isotype control, Neutralization, Neutralization Control, Tissue Culture, Western Blot, control for neutralization.


Filter By Application
Binding Control
(1)
Bioassay
(8)
Blocking
(1)
Cell Culture
(2)
ChIP
(3)
Co-Immunoprecipitation
(1)
Control
(110)
Flow Cytometry
(9)
Flow Cytometry Control
(3)
Functional Assay
(3)
ICC
(2)
ICC Control
(1)
IHC
(9)
IHC Control
(1)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(5)
Immunoprecipitation
(4)
In Vivo
(2)
Isotype Control
(13)
Isotype control
(1)
Neutralization
(39)
Neutralization Control
(14)
Tissue Culture
(1)
Western Blot
(1)
control for neutralization
(1)
All Applications
Filter By Species
Human
(96)
Mouse
(116)
Rat
(18)
Bacteria - Streptococcus pyogenes
(1)
Chicken
(2)
Guinea Pig
(1)
Hamster
(1)
Porcine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 244. Show All 244 Publications.
Publications using AB-108-C Applications Species
ML Tiirikaine, A Woetmann, H Norsgaard, LF Santamaria, P Lovato Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31948839] (Tissue Culture, Human) Tissue Culture Human
A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer&#039;s disease and aged brains: increased levels correlate with neuropathology Acta Neuropathol Commun, 2019;7(1):215. 2019 [PMID: 31864418] (Western Blot, Human) Western Blot Human
A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31689560] (Cell Culture, Human) Cell Culture Human
S Kinnear, LA Salamonsen, M Francois, V Harley, J Evans Uterine SOX17: a key player in human endometrial receptivity and embryo implantation Sci Rep, 2019;9(1):15495. 2019 [PMID: 31664088] (IHC, Human) IHC Human
S Mahmoudi, E Mancini, L Xu, A Moore, F Jahanbani, K Hebestreit, R Srinivasan, X Li, K Devarajan, L Prélot, CE Ang, Y Shibuya, BA Benayoun, ALS Chang, M Wernig, J Wysocka, MT Longaker, MP Snyder, A Brunet Heterogeneity in old fibroblasts is linked to variability in reprogramming and wound healing Nature, 2019;574(7779):553-558. 2019 [PMID: 31645721] (Cell Culture, Mouse) Cell Culture Mouse
N Charoenpha, R Aeimlapa, S Sooksawanw, J Thongbunch, J Teerapornp, S Svasti, K Wongdee Responses of primary osteoblasts and osteoclasts from hemizygous beta-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D3 Sci Rep, 2019;9(1):13963. 2019 [PMID: 31562377] (ICC, Mouse) ICC Mouse
A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep, 2019;28(4):896-908.e5. 2019 [PMID: 31340152] (Neutralization, Human) Neutralization Human
DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019;10(4):. 2019 [PMID: 31311882] (Neutralization, Human) Neutralization Human
N Inagaki-Ka, T Ito, M Inaba, Y Azuma, A Tanaka, PTX Vien, K Kibata, A Satake, S Nomura Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 31269241] (Isotype Control, Mouse) Isotype Control Mouse
LK Dubey, B Ludewig, SA Luther, NL Harris IL-4R?-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells Cell Rep, 2019;27(8):2442-2458.e5. 2019 [PMID: 31116987] (IHC-F, Mouse) IHC-F Mouse
Show All 244 Publications.

Reviews for IgG Isotype Control (AB-108-C) (4) 54

Average Rating: 5
(Based on 4 reviews)

Reviews using AB-108-C:
Filter by Applications
ICC/IF
(2)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC/IF 05/07/2019
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lotes4118071
 
reviewed by:
Anonymous
05/07/2019
View

Summary

ApplicationSerum
Lotes4118071
Immunocytochemistry/Immunofluorescence IgG AB-108-C
Enlarge
reviewed by:
Anonymous
ICC/IF 04/07/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.
Serum IgG AB-108-C
Enlarge
reviewed by:
Anonymous
04/07/2017
View

Summary

ApplicationSerum
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

 
Anonymous
05/07/2019
Application: ICC/IF
Species:

 
Anonymous
05/07/2019
Application:
Species:

Anonymous
04/07/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol IGHG1
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