Goat IgG Isotype Control [Unconjugated]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Reviewed Applications
Read 4 Reviews rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (AB-108-C)(256)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Chicken, Guinea Pig, Hamster, Porcine, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Xenograft.

We have publications tested in 26 applications: Binding Control, Bioassay, Blocking, Cell Culture, ChIP, Co-Immunoprecipitation, Control, Flow Cytometry, Flow Cytometry Control, Functional Assay, ICC, ICC Control, IHC, IHC Control, IHC-F, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Isotype Control, Isotype control, Neutralization, Neutralization Control, Tissue Culture, Western Blot, control for neutralization.


Filter By Application
Binding Control
(1)
Bioassay
(10)
Blocking
(1)
Cell Culture
(3)
ChIP
(3)
Co-Immunoprecipitation
(1)
Control
(111)
Flow Cytometry
(10)
Flow Cytometry Control
(3)
Functional Assay
(3)
ICC
(3)
ICC Control
(1)
IHC
(9)
IHC Control
(1)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(5)
Immunoprecipitation
(4)
In Vivo
(2)
Isotype Control
(15)
Isotype control
(1)
Neutralization
(41)
Neutralization Control
(15)
Tissue Culture
(1)
Western Blot
(2)
control for neutralization
(1)
All Applications
Filter By Species
Human
(106)
Mouse
(117)
Rat
(18)
Bacteria - Streptococcus pyogenes
(1)
Chicken
(2)
Guinea Pig
(1)
Hamster
(1)
Porcine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rabbit
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 256. Show All 256 Publications.
Publications using AB-108-C Applications Species
B Moon, J Lee, SA Lee, C Min, H Moon, D Kim, S Yang, H Moon, J Jeon, YE Joo, D Park Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis Cells, 2020;9(7):. 2020 [PMID: 32640697] (ICC, Human) ICC Human
C Castellini, S Mattioli, AD Bosco, E Cotozzolo, A Cartoni Ma, M Rende, AM Stabile, A Pistilli Nerve growth factor receptor role on rabbit sperm storage Theriogenology, 2020;153(0):54-61. 2020 [PMID: 32442740] (Flow Cytometry, Rabbit) Flow Cytometry Rabbit
A Klingler, D Regensburg, C Tenkerian, N Britzen-La, A Hartmann, M Stürzl, E Naschberge Species-, organ- and cell-type-dependent expression of SPARCL1 in human and mouse tissues PLoS ONE, 2020;15(5):e0233422. 2020 [PMID: 32437418] (Isotype Control, Human) Isotype Control Human
V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020;31(6):107615. 2020 [PMID: 32402278] (Control, Human) Control Human
J Kim, B Alejandro, M Hetman, EM Hattab, J Joiner, H Schroten, H Ishikawa, DH Chung Zika virus infects pericytes in the choroid plexus and enters the central nervous system through the blood-cerebrospinal fluid barrier PLoS Pathog., 2020;16(5):e1008204. 2020 [PMID: 32357162] (Neutralization, Human) Neutralization Human
D Bararia, JA Hildebrand, S Stolz, S Haebe, S Alig, CP Trevisani, F Osorio-Bar, MD Bartoschek, M Mentz, A Pastore, E Gaitzsch, M Heide, V Jurinovic, K Rautter, J Gunawardan, MB Sabdia, M Szczepanow, J Richter, W Klapper, A Louissaint, C Ludwig, S Bultmann, H Leonhardt, S Eustermann, KP Hopfner, W Hiddemann, M von Bergwe, C Steidl, R Kridel, JWD Tobin, MK Gandhi, DM Weinstock, M Schmidt-Su, MB Sárosi, M Rudelius, V Passerini, J Mautner, O Weigert Cathepsin S Alterations Induce a Tumor-Promoting Immune Microenvironment in Follicular Lymphoma Cell Rep, 2020;31(5):107522. 2020 [PMID: 32330423] (Western Blot, Human) Western Blot Human
L Wang, L Wang, H Zhang, J Lu, Z Zhang, H Wu, Z Liang AREG mediates the epithelial?mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF?&amp;kappaB signalling pathway Oncol. Rep., 2020;0(0):. 2020 [PMID: 32323797] (Bioassay, Human) Bioassay Human
K Kuronuma, A Yokoi, T Fukuoka, M Miyata, A Maekawa, S Tanaka, L Matsubara, C Goto, M Matsuo, HW Han, M Tsuruta, H Murata, H Okamoto, N Hasegawa, S Asano, M Ito Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 Heliyon, 2020;6(4):e03743. 2020 [PMID: 32322728] (Isotype Control, Mouse) Isotype Control Mouse
L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21 Aging (Albany NY), 2020;12(8):6823-6851. 2020 [PMID: 32302288] (Neutralization, Human) Neutralization Human
J Wang, C Ortiz, L Fontenot, Y Xie, W Ho, SA Mattai, DQ Shih, HW Koon High circulating elafin levels are associated with Crohn&#039;s disease-associated intestinal strictures PLoS ONE, 2020;15(4):e0231796. 2020 [PMID: 32287314] (Bioassay, Human) Bioassay Human
Show All 256 Publications.

Reviews for IgG Isotype Control (AB-108-C) (4) 54

Average Rating: 5
(Based on 4 reviews)

Reviews using AB-108-C:
Filter by Applications
ICC/IF
(2)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
ICC/IF 05/07/2019
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lotes4118071
  5
reviewed by:
Anonymous
05/07/2019
View

Summary

ApplicationSerum
Lotes4118071
Immunocytochemistry/Immunofluorescence IgG AB-108-C
Enlarge
5
reviewed by:
Anonymous
ICC/IF 04/07/2017
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.
Serum IgG AB-108-C
Enlarge
5
reviewed by:
Anonymous
04/07/2017
View

Summary

ApplicationSerum
LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

 
Anonymous
05/07/2019
Application: ICC/IF
Species:

 
Anonymous
05/07/2019
Application:
Species:

Anonymous
04/07/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol IGHG1