ROBO4 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

ROBO4 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal region of human Robo4 (within residues 200-300). [Swiss-Prot# Q8WZ75]
Localization
Cell Membrane, cytoplasmic vesicles
Marker
Tumor Endothelial Marker
Specificity
This recognizes the 85 kDa and 107 kDa isoforms of the ROBO4 human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROBO4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Robo4 antibody is useful for Immunohistochemistry-Paraffin and Western Blot analysis, where bands at 85 kDa and 107 kDa are observed representing different isoforms of ROBO4 protein.
Readout System

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ROBO4 Antibody [Biotin]

  • ECSM4
  • FLJ20798
  • Magic roundabout
  • MGC133352
  • MGC133353
  • MRB
  • ROBO4
  • roundabout homolog 4
  • roundabout homolog 4, magic roundabout (Drosophila)

Background

Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development. ROBO4 is a novel roundabout protein which is restricted in expression to endothelial cells in vitro and sites of angiogenesis in vivo. Because it is not expressed on normal endothelial cells, in vivo, it is a promising tumor endothelial marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB110-58778G
DyLight 550 Labeled NB110-58778R
DyLight 650 Labeled NB110-58778C
HRP Labeled NB110-58778H

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Bioinformatics Tool for ROBO4 Antibody (NB110-58778B)

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Blogs on ROBO4

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Contact Information

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Bioinformatics

Gene Symbol ROBO4
Entrez