Mineralocorticoid R/NR3C2 Antibody (H10E4C9F)

Images

 
Western Blot: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Analysis of Mineralocorticoid Receptor.
Immunocytochemistry/ Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is ...read more
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is ...read more
Immunocytochemistry/ Immunofluorescence: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is ...read more
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) [NB300-562] - Both normal and cancer biopsies of deparaffinized Human kidney tissue tissues

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, B/N
Clone
H10E4C9F
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.4 mg/ml

Mineralocorticoid R/NR3C2 Antibody (H10E4C9F) Summary

Immunogen
Aldosterone-3
Specificity
Detects mineralocorticoid receptor (MR).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NR3C2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:20
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Block/Neutralize
Application Notes
Useful in IHC, ICC, and WB. Not suitable for IP. WB: Detects an approx. 116 kDa protein representing MR in rat heart homogenate. IHC: Staining of MR in rabbit atrium with NB300-562 results in strong staining of myocytes and endothelial cells. IHC: Staining with NB300-562 is blocked by pre-incubating the sample with aldosterone. NB300-562 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for WBting such as enhanced chemiluminescence. NB300-562 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor.
Publications
Read Publication using
NB300-562 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 12198239). Sheep reactivity reported in scientific literature (PMID: 5695102).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.4 mg/ml
Purity
Protein A purified

Alternate Names for Mineralocorticoid R/NR3C2 Antibody (H10E4C9F)

  • FLJ41052
  • MGC133092
  • Mineralocorticoid R
  • mineralocorticoid receptor 1
  • mineralocorticoid receptor delta
  • MineralocorticoidR
  • MLR
  • MLRmineralocorticoid receptor
  • MRMCRaldosterone receptor
  • NR3C2
  • NR3C2VIT
  • Nuclear receptor subfamily 3 group C member 2
  • nuclear receptor subfamily 3, group C, member 2

Background

Steroid receptors are ligand dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain Mineralocorticoid Receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ch, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, B/N

Publications for Mineralocorticoid R/NR3C2 Antibody (NB300-562)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mineralocorticoid R/NR3C2 Antibody (NB300-562) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-562AF350
Alexa Fluor 405 NB300-562AF405
Alexa Fluor 488 NB300-562AF488
Alexa Fluor 532 NB300-562AF532
Alexa Fluor 594 NB300-562AF594
Alexa Fluor 647 NB300-562AF647
Alexa Fluor 700 NB300-562AF700
Alexa Fluor 750 NB300-562AF750
Allophycocyanin NB300-562APC
Allophycocyanin/Cy7 NB300-562APCCY7
DyLight 350 NB300-562UV
DyLight 405 NB300-562V
DyLight 488 NB300-562G
DyLight 594 NB300-562DL594
DyLight 650 NB300-562C
DyLight 680 NB300-562FR
DyLight 755 NB300-562IR
Janelia Fluor 549 NB300-562JF549
Janelia Fluor 646 NB300-562JF646
PE NB300-562PE
PE/Atto594 NB300-562PEATT594
PE/Cy5.5 NB300-562PECY55
PE/Cy7 NB300-562PECY7

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Bioinformatics

Gene Symbol NR3C2
Entrez
Uniprot