ACE-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

ACE-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1
Specificity
Detects human ACE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACE2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF933 in the following applications:

Publications
Read Publications using
AF933 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1513
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
NBP1-78444
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut
NBP1-77078
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15771
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-59882
Species: Hu
Applications: WB, IHC
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF4965
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-22449
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, GS

Publications for ACE-2 Antibody (AF933)(17)

We have publications tested in 5 confirmed species: Human, Mouse, Bat, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 7 applications: Flow, ICC, IF, IHC Not Specified, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Flow
(3)
ICC
(2)
IF
(2)
IHC Not Specified
(1)
IHC Paraffin-embedded
(1)
Neut
(3)
WB
(5)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Bat
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(5)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF933 Applications Species
Aleksandra Milewska HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016;11(6):e0156552. 2016 [PMID: 27249425] (IHC Not Specified,, Primate - Macaca mulatta (Rhesus Macaque)) IHC Not Specified Primate - Macaca mulatta (Rhesus Macaque)
Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 2016;23(1):14. 2016 [PMID: 26801988] (Neut,, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Neut Primate - Chlorocebus pygerythrus (Vervet Monkey)
Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 2014;9(1):e85958. 2014 [PMID: 24454948] (WB,, Mouse) WB Mouse
Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013;10(0):359. 2013 [PMID: 24364985] (IF,, Human) IF Human
Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Flow,, Human) Flow Human
Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature, 2013;503(7477):535-8. 2013 [PMID: 24172901] (IF,, Bat) IF Bat
Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 2012;7(4):e35876. 2012 [PMID: 22558251] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010;285(53):41935-46. 2010 [PMID: 20966072] (WB,, Mouse) WB Mouse
Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology, 2008;381(2):215-21. 2008 [PMID: 18814896] (WB,, Primate - Chlorocebus pygerythrus (Vervet Monkey)) WB Primate - Chlorocebus pygerythrus (Vervet Monkey)
Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 2008;52(9):750-4. 2008 [PMID: 18718423] (Neut,, Human) Neut Human
Show All 17 Publications.

Review for ACE-2 Antibody (AF933) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF933:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23232719
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23232719

Details

Detection NotesSee PMID 23232719

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23232719.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ACE-2 Antibody (AF933) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ACE-2 Products

Bioinformatics Tool for ACE-2 Antibody (AF933)

Discover related pathways, diseases and genes to ACE-2 Antibody (AF933). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ACE-2 Antibody (AF933)

Discover more about diseases related to ACE-2 Antibody (AF933).
 

Pathways for ACE-2 Antibody (AF933)

View related products by pathway.

PTMs for ACE-2 Antibody (AF933)

Learn more about PTMs related to ACE-2 Antibody (AF933).

Blogs on ACE-2

There are no specific blogs for ACE-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

ACE-2
933-ZN-010

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
01/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACE2
Entrez
Uniprot