ACE-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen ...read more
HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was stained with (A) Goat Anti-Human ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) or (B) Goat IgG control antibody (Catalog ...read more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

ACE-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ACE-2
Gln18-Ser740
Accession # Q9BYF1
Specificity
Detects human ACE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACE2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 5
using
AF933 in the following applications:

Publications
Read Publications using
AF933 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC 3.4.17.23
  • Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Li, W. et al. (2003) Nature 426:450.
  5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE-2 Antibody (AF933)(22)

We have publications tested in 7 confirmed species: Human, Mouse, Bat, Primate - Cercopithecus aethiops (African Green Monkey), Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rhesus Macaca (Macaque).

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(2)
IHC
(3)
IHC-P
(1)
Neutralization
(4)
Western Blot
(7)
All Applications
Filter By Species
Human
(9)
Mouse
(3)
Bat
(1)
Primate - Cercopithecus aethiops (African Green Monkey)
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(5)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rhesus Macaca (Macaque)
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF933 Applications Species
Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun, 0;11(1):1620. 0 [PMID: 32221306] (Flow Cytometry, Human) Flow Cytometry Human
Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(0):1-10. 2020 [PMID: 32142651] (Neutralization, Primate - Cercopithecus aethiops (African Green Monkey)) Neutralization Primate - Cercopithecus aethiops (African Green Monkey)
D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 2019;9(1):9193. 2019 [PMID: 31235714] (Rhesus Macaca (Macaque)) Rhesus Macaca (Macaque)
E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 2017;7(0):350-365. 2017 [PMID: 28624211] (Western Blot, Human) Western Blot Human
Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 2016;0(0):1663-9812. 2016 [PMID: 27313531] (Western Blot, Mouse) Western Blot Mouse
Aleksandra Milewska HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016;11(6):e0156552. 2016 [PMID: 27249425] (IHC, Primate - Macaca mulatta (Rhesus Macaque)) IHC Primate - Macaca mulatta (Rhesus Macaque)
Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 2016;23(1):14. 2016 [PMID: 26801988] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Neutralization Primate - Chlorocebus pygerythrus (Vervet Monkey)
Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 2014;9(1):e85958. 2014 [PMID: 24454948] (Western Blot, Mouse) Western Blot Mouse
Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013;10(0):359. 2013 [PMID: 24364985] (IHC, Human) IHC Human
Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Flow Cytometry, Human) Flow Cytometry Human
Show All 22 Publications.

Review for ACE-2 Antibody (AF933) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF933:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 01/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23232719
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23232719

Details

Detection NotesSee PMID 23232719

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23232719.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ACE-2 Antibody (AF933) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP3-00487AF350
Alexa Fluor 488 NBP3-00487AF488
Alexa Fluor 532 NBP3-00487AF532
Alexa Fluor 594 NBP3-00487AF594
Alexa Fluor 647 NBP3-00487AF647
Alexa Fluor 700 NBP3-00487AF700
Alexa Fluor 750 NBP3-00487AF750
Allophycocyanin NBP3-00487APC
Biotin BAF933
DyLight 350 NBP3-00487UV
DyLight 405 NBP3-00487V
DyLight 594 NBP3-00487DL594
DyLight 650 NBP3-00487C
DyLight 680 NBP3-00487FR
DyLight 755 NBP3-00487IR
FITC NBP3-00487F
HRP NBP3-00487H
Janelia Fluor 549 NBP3-00487JF549
Janelia Fluor 646 NBP3-00487JF646
PE NBP3-00487PE
PerCP NBP3-00487PCP

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Bioinformatics Tool for ACE-2 Antibody (AF933)

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Blogs on ACE-2.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Recent Reviews

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Anonymous
01/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACE2
Uniprot