ACE-2 Antibody [Unconjugated]


Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal more
HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at more
ACE‑2 was detected in immersion fixed frozen sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at more
Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen more

Product Details

Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, B/N

ACE-2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Accession # Q9BYF1
Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Blockade of Receptor-ligand Interaction
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
AF933 in the following applications:

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AF933 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC
  • Metalloprotease MPROT15


ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Li, W. et al. (2003) Nature 426:450.
  5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
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Applications: ELISA, ICC/IF, KD, WB
Species: Ca, Hu, Ma, Mu, Rt
Applications: ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Pm, Mu, Rt
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Applications: ELISA
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Applications: IHC, Simple Western, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-FrFl, PEP-ELISA, WB
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Applications: ELISA

Publications for ACE-2 Antibody (AF933)(114)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, African Green Monkey, Bat, Civet, Mesocricetus auratus (Hamster), Mink, Primate, Primate - C. aethiops, Primate - Cercopithecus aethiops (African Green Monkey), Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rhesus Macaca (Macaque), Transgenic Mouse.

We have publications tested in 11 applications: Co-Immunoprecipitation, Control, ELISA Detection, FLOW, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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African Green Monkey
Mesocricetus auratus (Hamster)
Primate - C. aethiops
Primate - Cercopithecus aethiops (African Green Monkey)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
Rhesus Macaca (Macaque)
Transgenic Mouse
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Showing Publications 1 - 10 of 114. Show All 114 Publications.
Publications using AF933 Applications Species
Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA; Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface The Journal of biological chemistry 2023-05-13 [PMID: 37187291] (Flow Cytometry, Human) Flow Cytometry Human
Barreto, EA;Cruz, AS;Veras, FP;Martins, R;Bernardelli, RS;Paiva, IM;Lima, TM;Singh, Y;Guimar�es, RC;Damasceno, S;Pereira, N;Alves, JM;Gon�alves, TT;Forato, J;Muraro, SP;Souza, GF;Batah, SS;Proenca-Modena, JL;Mori, MA;Cunha, FQ;Louzada-Junior, P;Cunha, TM;Nakaya, HI;Fabro, A;de Oliveira, RDR;Arruda, E;R�a, R;R�a Neto, �;Fernandes da Silva, MM;Leiria, LO; COVID-19-related hyperglycemia is associated with infection of hepatocytes and stimulation of gluconeogenesis Proceedings of the National Academy of Sciences of the United States of America 2023-05-23 [PMID: 37186819] (Neutralization, Human) Neutralization Human
M Ulinici, A Sulji?, M Poggianell, R Milan Bono, K Resman Rus, A Paraschiv, AM Bonetti, M Todiras, A Corlateanu, S Groppa, E Ceban, M Petrovec, A Marcello Characterisation of the Antibody Response in Sinopharm (BBIBP-CorV) Recipients and COVID-19 Convalescent Sera from the Republic of Moldova Vaccines, 2023-03-13;11(3):. 2023-03-13 [PMID: 36992221] (FLOW, Human) FLOW Human
AT Tang, DW Buchholz, KM Szigety, B Imbiakha, S Gao, M Frankfurte, M Wang, J Yang, P Hewins, P Mericko-Is, NA Leu, S Sterling, IA Monreal, J Sahler, A August, X Zhu, KA Jurado, M Xu, EE Morrisey, SE Millar, HC Aguilar, ML Kahn Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PloS Biology, 2023-02-06;21(2):e3001989. 2023-02-06 [PMID: 36745682] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
N Tailor, BM Warner, BD Griffin, K Tierney, E Moffat, K Frost, R Vendramell, A Leung, M Willman, SP Thomas, Y Pei, SA Booth, C Embury-Hya, SK Wootton, D Kobasa Generation and Characterization of a SARS-CoV-2-Susceptible Mouse Model Using Adeno-Associated Virus (AAV6.2FF)-Mediated Respiratory Delivery of the Human ACE2 Gene Viruses, 2022-12-28;15(1):. 2022-12-28 [PMID: 36680125] (Western Blot, Human) Western Blot Human
Y Kato, K Nishiyama, J Man Lee, Y Ibuki, Y Imai, T Noda, N Kamiya, T Kusakabe, Y Kanda, M Nishida TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation International Journal of Molecular Sciences, 2022-12-21;24(1):. 2022-12-21 [PMID: 36613540] (Western Blot, Mouse) Western Blot Mouse
A Miluzio, A Cuomo, C Cordiglier, L Donnici, E Pesce, M Bombaci, M Conti, A Fasciani, L Terraccian, L Manganaro, M Toccafondi, A Scagliola, S Oliveto, S Ricciardi, R Grifantini, R De Frances, S Abrignani, N Manfrini, S Biffo Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2 EBioMedicine, 2022-12-28;87(0):104390. 2022-12-28 [PMID: 36584595] (Co-Immunoprecipitation, Flow Cytometry, IHC, Western Blot, Human) Co-Immunoprecipitation, Flow Cytometry, IHC, Western Blot Human
X Guo, J Cao, JP Cai, J Wu, J Huang, P Asthana, SKK Wong, ZW Ye, S Gurung, Y Zhang, S Wang, Z Wang, X Ge, HY Kwan, A Lyu, KM Chan, N Wong, J Huang, Z Zhou, ZX Bian, S Yuan, HLX Wong Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2 Nature Communications, 2022-12-23;13(1):7907. 2022-12-23 [PMID: 36564389] (ICC, Human) ICC Human
DR Adney, J Lovaglio, JE Schulz, CK Yinda, VA Avanzato, E Haddock, JR Port, MG Holbrook, PW Hanley, G Saturday, D Scott, C Shaia, AM Nelson, JR Spengler, C Tansey, CM Cossaboom, NM Wendling, C Martens, J Easley, SW Yap, SN Seifert, VJ Munster Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2 JCI Insight, 2022-11-22;7(22):. 2022-11-22 [PMID: 36509288] (IHC, Mink) IHC Mink
SB Biering, FT Gomes de S, LV Tjang, F Pahmeier, C Zhu, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, DM Fox, V Ortega, AM Näär, RS Baric, SA Stanley, HC Aguilar, JD Esko, CY Chiu, JE Pak, PR Beatty, E Harris SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling Nature Communications, 2022-12-09;13(1):7630. 2022-12-09 [PMID: 36494335] (Western Blot, Human) Western Blot Human
Show All 114 Publications.

Review for ACE-2 Antibody (AF933) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF933:
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reviewed by:
cynthia morimoto
IF Human 01/12/2015


Sample TestedSee PMID 23232719

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Blogs on ACE-2.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

COVID-19 and the Cardiovascular System: Observed complications and potential mechanisms
By Victoria OsinskiThe outbreak of COVID-19 resulting from the transmission of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in many cases of illness typically manifesting in mi...  Read full blog post.

  Read full blog post.

  Read full blog post.

Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Recent Reviews


cynthia morimoto
Application: IF
Species: Human


Gene Symbol ACE2