ACE-2 Antibody [Unconjugated]

Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was stained with (A) Goat Anti-Human ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) or (B) Goat IgG control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, Simple Western, Flow, IHC, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ACE-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
4. Li, W. et al. (2003) Nature 426:450.
5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (AF933)(79)

Publications using AF933

• Y Menuchin-L, A Schreiber, A Lecanda, A Mecate-Zam, L Brunotte, OE Psathaki, S Ludwig, T Rauen, HR Schöler SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids Stem Cell Reports, 2022;0(0):. 2022 [PMID: 35334213] (Neutralization, Human)
• J Shi, X Jin, Y Ding, X Liu, A Shen, Y Wu, M Peng, C Shen Receptor-Binding Domain Proteins of SARS-CoV-2 Variants Elicited Robust Antibody Responses Cross-Reacting with Wild-Type and Mutant Viruses in Mice Vaccines, 2021;9(12):. 2021 [PMID: 34960131] (ELISA Detection, Human)
• SB Biering, FTG de Sousa, LV Tjang, F Pahmeier, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, V Ortega, HC Aguilar, JD Esko, CY Chui, JE Pak, PR Beatty, E Harris SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling bioRxiv : the preprint server for biology, 2021;0(0):. 2021 [PMID: 34931188] (Western Blot, Human)
• J Wang, G Yang, X Wang, Z Wen, L Shuai, J Luo, C Wang, Z Sun, R Liu, J Ge, X He, R Hua, X Wang, X Yang, W Chen, G Zhong, Z Bu SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells Cell Discovery, 2021;7(1):119. 2021 [PMID: 34903715] (Flow Cytometry, Human)
• L Helms, S Marchiano, IB Stanaway, TY Hsiang, BA Juliar, S Saini, YT Zhao, A Khanna, R Menon, F Alakwaa, C Mikacenic, ED Morrell, MM Wurfel, M Kretzler, JL Harder, CE Murry, J Himmelfarb, H Ruohola-Ba, PK Bhatraju, M Gale, BS Freedman Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations JCI Insight, 2021;0(0):. 2021 [PMID: 34767537] (IHC, Human)
• L Nguyen, KA McCord, DT Bui, KM Bouwman, EN Kitova, M Elaish, D Kumawat, GC Daskhan, I Tomris, L Han, P Chopra, TJ Yang, SD Willows, AL Mason, LK Mahal, TL Lowary, LJ West, SD Hsu, T Hobman, SM Tompkins, GJ Boons, RP de Vries, MS Macauley, JS Klassen Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2 Nature Chemical Biology, 2021;0(0):. 2021 [PMID: 34754101] (Flow Cytometry, Human)
• X Duan, X Tang, MS Nair, T Zhang, Y Qiu, W Zhang, P Wang, Y Huang, J Xiang, H Wang, RE Schwartz, DD Ho, T Evans, S Chen An airway organoid-based screen identifies a role for the HIF1alpha-glycolysis axis in SARS-CoV-2 infection Cell Reports, 2021;37(6):109920. 2021 [PMID: 34731648] (IHC, Human)
• MW Ku, P Authié, M Bourgine, F Anna, A Noirat, F Moncoq, B Vesin, F Nevo, J Lopez, P Souque, C Blanc, I Fert, S Chardenoux, L Lafosse, D Cussigh, D Hardy, K Nemirov, F Guinet, F Langa Vive, L Majlessi, P Charneau Brain Cross-Protection against SARS-CoV-2 Variants by a Lentiviral Vaccine in New Transgenic Mice Embo Molecular Medicine, 2021;0(0):e14459. 2021 [PMID: 34647691] (Western Blot, Transgenic Mouse)
• S Singh, G Garcia, R Shah, AA Kramerov, RE Wright, TM Spektor, AV Ljubimov, V Arumugaswa, A Kumar SARS-CoV-2 and its variants of concern infect human conjunctival epithelial cells and induce differential antiviral innate immune response The ocular surface, 2021;0(0):. 2021 [PMID: 34583089] (ICC, Human)
• SP Shukla, KB Cho, V Rustagi, X Gao, X Fu, SX Zhang, B Guo, DG Udugamasoo "Molecular Masks" for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus Entry International Journal of Molecular Sciences, 2021;22(16):. 2021 [PMID: 34445669] (Western Blot, Human)
• EJ Sherman, BT Emmer ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes Scientific Reports, 2021;11(1):15900. 2021 [PMID: 34354120] (Western Blot, Human)
• CLC Almeida-da, H Matshik Da, K Liu, DM Ojcius Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells International Journal of Molecular Sciences, 2021;22(14):. 2021 [PMID: 34299289] (Western Blot, Human)
• Y Kondo, JL Larabee, L Gao, H Shi, B Shao, CM Hoover, JM McDaniel, YC Ho, R Silasi-Man, SA Archer-Har, P Azadi, RS Srinivasan, AR Rezaie, A Borczuk, JC Laurence, F Lupu, J Ahamed, RP McEver, JF Papin, Z Yu, L Xia L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus JCI Insight, 2021;6(14):. 2021 [PMID: 34291736] (IHC, Human)
• L Wang, D Sievert, AE Clark, S Lee, H Federman, BD Gastfriend, EV Shusta, SP Palecek, AF Carlin, JG Gleeson A human three-dimensional neural-perivascular 'assembloid' promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology Nature Medicine, 2021;0(0):. 2021 [PMID: 34244682] (IHC, Human)
• M Puray-Chav, KM LaPak, TP Schrank, JL Elliott, DP Bhatt, MJ Agajanian, R Jasuja, DQ Lawson, K Davis, PW Rothlauf, Z Liu, H Jo, N Lee, K Tenneti, JE Eschbach, C Shema Mugi, EM Cousins, EW Cloer, HR Vuong, LA VanBlargan, AL Bailey, P Gilchuk, JE Crowe, MS Diamond, DN Hayes, SPJ Whelan, A Horani, SL Brody, D Goldfarb, MB Major, SB Kutluay Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell Cell Reports, 2021;0(0):109364. 2021 [PMID: 34214467] (Western Blot, Human)
• M Zhao, PY Su, DA Castro, TN Tripler, Y Hu, M Cook, AI Ko, SF Farhadian, B Israelow, CS Dela Cruz, Y Xiong, RE Sutton, Yale IMPAC Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 PloS Pathogens, 2021;17(6):e1009683. 2021 [PMID: 34166473] (Western Blot, Human)
• X Wang, CH Chen, S Badeti, JH Cho, A Naghizadeh, Z Wang, D Liu Deletion of ER-retention motif on SARS-CoV-2 spike protein reduces cell hybrid during cell-cell fusion Cell & bioscience, 2021;11(1):114. 2021 [PMID: 34162440] (Flow Cytometry, Human)
• AZ Eriksen, R Møller, B Makovoz, SA Uhl, BR tenOever, TA Blenkinsop SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34022129] (IHC, Human)
• CH Chen, S Badeti, JH Cho, A Naghizadeh, X Wang, D Liu Deletion of ER-retention Motif on SARS-CoV-2 Spike Protein Reduces Cell Hybrid During Cell-cell Fusion Research square, 2021;0(0):. 2021 [PMID: 33851149] (Flow Cytometry, Human)
• M Dittmar, JS Lee, K Whig, E Segrist, M Li, B Kamalia, L Castellana, K Ayyanathan, FL Cardenas-D, EE Morrisey, R Truitt, W Yang, K Jurado, K Samby, H Ramage, DC Schultz, S Cherry Drug repurposing screens reveal cell-type-specific entry pathways and FDA-approved drugs active against SARS-Cov-2 Cell Reports, 2021;35(1):108959. 2021 [PMID: 33811811] (Western Blot, Human)
• SJ Dicken, MJ Murray, LG Thorne, AK Reuschl, C Forrest, M Ganeshalin, L Muir, MD Kalemera, M Palor, LE McCoy, C Jolly, GJ Towers, MB Reeves, J Grove Characterisation of B.1.1.7 and Pangolin coronavirus spike provides insights on the evolutionary trajectory of SARS-CoV-2 bioRxiv : the preprint server for biology, 2021;0(0):. 2021 [PMID: 33791702] (Western Blot, Human)
• Y Zhang, R Guo, SH Kim, H Shah, S Zhang, JH Liang, Y Fang, M Gentili, CNO Leary, SJ Elledge, DT Hung, VK Mootha, BE Gewurz SARS-CoV-2 hijacks folate and one-carbon metabolism for viral replication Nature Communications, 2021;12(1):1676. 2021 [PMID: 33723254] (Flow Cytometry, Human)
• J Baggen, L Persoons, E Vanstreels, S Jansen, D Van Loover, B Boeckx, V Geudens, J De Man, D Jochmans, J Wauters, E Wauters, BM Vanaudenae, D Lambrechts, J Neyts, K Dallmeier, HJ Thibaut, M Jacquemyn, P Maes, D Daelemans Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2 Nature Genetics, 2021;0(0):. 2021 [PMID: 33686287] (Western Blot, Human)
• K Uemura, M Sasaki, T Sanaki, S Toba, Y Takahashi, Y Orba, WW Hall, K Maenaka, H Sawa, A Sato MRC5 cells engineered to express ACE2 serve as a model system for the discovery of antivirals targeting SARS-CoV-2 Scientific Reports, 2021;11(1):5376. 2021 [PMID: 33686154] (Flow Cytometry, Human)
• EE Khoury, Y Knaney, A Fokra, S Kinaneh, Z Azzam, SN Heyman, Z Abassi Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease Journal of Cellular and Molecular Medicine, 2021;0(0):. 2021 [PMID: 33660945] (Rat)
• S Sugimoto, E Kobayashi, M Fujii, Y Ohta, K Arai, M Matano, K Ishikawa, K Miyamoto, K Toshimitsu, S Takahashi, K Nanki, Y Hakamata, T Kanai, T Sato An organoid-based organ-repurposing approach to treat short bowel syndrome Nature, 2021;0(0):. 2021 [PMID: 33627870] (IHC, Rat)
• H Huerga Enc, W Grey, M Garcia-Alb, H Wood, R Ulferts, IV Aramburu, AG Kulasekara, G Mufti, V Papayannop, R Beale, D Bonnet Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 Patients Stem Cell Reports, 2021;16(3):428-436. 2021 [PMID: 33581053] (Flow Cytometry, Human)
• F Onodi, L Bonnet-Mad, L Meertens, L Karpf, J Poirot, SY Zhang, C Picard, A Puel, E Jouanguy, Q Zhang, J Le Goff, JM Molina, C Delaugerre, JL Casanova, A Amara, V Soumelis SARS-CoV-2 induces human plasmacytoid pre-dendritic cell diversification via UNC93B and IRAK4 bioRxiv : the preprint server for biology, 2021;0(0):. 2021 [PMID: 33442685] (Flow Cytometry, Human)
• X Yin, L Riva, Y Pu, L Martin-San, J Kanamune, Y Yamamoto, K Sakai, S Gotoh, L Miorin, PD De Jesus, CC Yang, KM Herbert, S Yoh, JF Hultquist, A García-Sas, SK Chanda MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells Cell Reports, 2021;34(2):108628. 2021 [PMID: 33440148] (ICC, Human)
• C Blume, CL Jackson, CM Spalluto, J Legebeke, L Nazlamova, F Conforti, JM Perotin, M Frank, J Butler, M Crispin, J Coles, J Thompson, RA Ridley, LSN Dean, M Loxham, S Reikine, A Azim, K Tariq, DA Johnston, PJ Skipp, R Djukanovic, D Baralle, CJ McCormick, DE Davies, JS Lucas, G Wheway, V Mennella A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection Nature Genetics, 2021;0(0):. 2021 [PMID: 33432184] (ICC, Human)
• H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen Host and viral determinants for efficient SARS-CoV-2 infection of the human lung Nature Communications, 2021;12(1):134. 2021 [PMID: 33420022] (Flow Cytometry, Mesocricetus auratus (Hamster))
• AZ Mykytyn, TI Breugem, S Riesebosch, D Schipper, PB van den Do, RJ Rottier, MM Lamers, BL Haagmans SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site Elife, 2021;10(0):. 2021 [PMID: 33393462] (ICC, Human)
• RM Samuel, H Majd, MN Richter, Z Ghazizadeh, SM Zekavat, A Navickas, JT Ramirez, H Asgharian, CR Simoneau, LR Bonser, KD Koh, M Garcia-Kni, M Tassetto, S Sunshine, S Farahvashi, A Kalantari, W Liu, R Andino, H Zhao, P Natarajan, DJ Erle, M Ott, H Goodarzi, F Fattahi Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men Cell Stem Cell, 2020;27(6):876-889.e12. 2020 [PMID: 33232663] (ICC, Human)
• JK Osan, SN Talukdar, F Feldmann, B Ann DeMont, K Jerome, KL Bailey, H Feldmann, M Mehedi Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD bioRxiv, 2020;0(0):. 2020 [PMID: 33200131] (Western Blot, Human)
• R Tandon, D Mitra, P Sharma, MG McCandless, SJ Stray, JT Bates, GD Marshall Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein Sci Rep, 2020;10(1):19076. 2020 [PMID: 33154514] (Western Blot, Human)
• IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schürch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, H Chen, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, A Tzankov, MS Matter, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs Nat Commun, 2020;11(1):5453. 2020 [PMID: 33116139] (IHC, Human)
• L Pellegrini, A Albecka, DL Mallery, MJ Kellner, D Paul, AP Carter, LC James, MA Lancaster SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33113348] (Western Blot, Human)
• W Rut, K Groborz, L Zhang, X Sun, M Zmudzinski, B Pawlik, X Wang, D Jochmans, J Neyts, W M?ynarski, R Hilgenfeld, M Drag SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging Nat Chem Biol, 2020;0(0):. 2020 [PMID: 33093684] (ICC, Human)
• L Casciola-R, DR Thiemann, F Andrade, MI Trejo Zamb, JE Hooper, E Leonard, J Spangler, AL Cox, C Machamer, L Sauer, O Laeyendeck, BT Garibaldi, SC Ray, C Mecoli, L Christophe, L Gutierrez-, Q Yang, D Hines, W Clarke, RE Rothman, A Pekosz, K Fenstermac, Z Wang, SL Zeger, A Rosen IgM autoantibodies recognizing ACE2 are associated with severe COVID-19 medRxiv, 2020;0(0):. 2020 [PMID: 33083808] (Western Blot, Human)
• M Baratchian, JM McManus, M Berk, F Nakamura, S Mukhopadhy, W Xu, S Erzurum, J Drazba, J Peterson, EA Klein, B Gaston, N Sharifi Sex, androgens and regulation of pulmonary AR, TMPRSS2 and ACE2 bioRxiv, 2020;0(0):. 2020 [PMID: 33083800] (IHC, Mouse)
• X Xie, AE Muruato, X Zhang, KG Lokugamage, CR Fontes-Gar, J Zou, J Liu, P Ren, M Balakrishn, T Cihlar, CK Tseng, S Makino, VD Menachery, JP Bilello, PY Shi A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19 Nat Commun, 2020;11(1):5214. 2020 [PMID: 33060595] (ICC, Primate)
• SP Sajuthi, P DeFord, Y Li, ND Jackson, MT Montgomery, JL Everman, CL Rios, E Pruesse, JD Nolin, EG Plender, ME Wechsler, ACY Mak, C Eng, S Salazar, V Medina, EM Wohlford, S Huntsman, DA Nickerson, S Germer, MC Zody, G Abecasis, HM Kang, KM Rice, R Kumar, S Oh, J Rodriguez-, EG Burchard, MA Seibold Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium Nat Commun, 2020;11(1):5139. 2020 [PMID: 33046696] (IHC, Human)
• R Wang, CR Simoneau, J Kulsuptrak, M Bouhaddou, K Travisano, JM Hayashi, J Carlson-St, J Oki, K Holden, NJ Krogan, M Ott, AS Puschnik Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses bioRxiv : the preprint server for biology, 2020;0(0):. 2020 [PMID: 32995787] (Western Blot, `, Human)
• J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Lindstrom-, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32979316] (Flow Cytometry, Human)
• E Song, C Zhang, B Israelow, A Lu-Culliga, AV Prado, S Skriabine, P Lu, OE Weizman, F Liu, Y Dai, K Szigeti-Bu, Y Yasumoto, G Wang, C Castaldi, J Heltke, E Ng, J Wheeler, MM Alfajaro, E Levavasseu, B Fontes, NG Ravindra, D Van Dijk, S Mane, M Gunel, A Ring, SA Jaffar Kaz, K Zhang, CB Wilen, TL Horvath, I Plu, S Haik, JL Thomas, A Louvi, SF Farhadian, A Huttner, D Seilhean, N Renier, K Bilguvar, A Iwasaki Neuroinvasion of SARS-CoV-2 in human and mouse brain bioRxiv : the preprint server for biology, 2020;0(0):. 2020 [PMID: 32935108] (Neutralization, Human)
• I Busnadiego, S Fernbach, MO Pohl, U Karakus, M Huber, A Trkola, S Stertz, BG Hale Antiviral Activity of Type I, II, and III Interferons Counterbalances ACE2 Inducibility and Restricts SARS-CoV-2 MBio, 2020;11(5):. 2020 [PMID: 32913009] (Flow Cytometry, Human)
• T Azad, R Singaravel, MJF Crupi, T Jamieson, J Dave, EEF Brown, R Rezaei, Z Taha, S Boulton, NT Martin, A Surendran, J Poutou, M Ghahremani, K Nouri, JT Whelan, J Duong, S Tucker, JS Diallo, JC Bell, CS Ilkow Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization Membranes (Basel), 2020;10(9):. 2020 [PMID: 32872641] (Western Blot, Human)
• F Duan, L Guo, L Yang, Y Han, A Thakur, BE Nilsson-Pa, P Wang, Z Zhang, CY Ma, X Zhou, T Han, T Zhang, X Wang, D Xu, X Duan, J Xiang, HF Tse, C Liao, W Luo, FP Huang, YW Chen, T Evans, RE Schwartz, B tenOever, DD Ho, S Chen, Q Lian, HJ Chen Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2 Res Sq, 2020;0(0):. 2020 [PMID: 32839764] (IHC, Mouse)
• R Rathnasing, S Strohmeier, F Amanat, VL Gillespie, F Krammer, A García-Sas, L Coughlan, M Schotsaert, M Uccellini Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection bioRxiv, 2020;0(0):. 2020 [PMID: 32676603] (Flow Cytometry, Human)
• J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response bioRxiv, 2020;0(0):. 2020 [PMID: 32637964] (Flow Cytometry, Human)
• J Prévost, R Gasser, G Beaudoin-B, J Richard, R Duerr, A Laumaea, SP Anand, G Goyette, S Ding, H Medjahed, A Lewin, J Perreault, T Tremblay, G Gendron-Le, N Gauthier, M Carrier, D Marcoux, A Piché, M Lavoie, A Benoit, V Loungnarat, G Brochu, M Desforges, PJ Talbot, GT Gould Maul, M Côté, C Therrien, B Serhir, R Bazin, M Roger, A Finzi Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike bioRxiv : the preprint server for biology, 2020;0(0):. 2020 [PMID: 32577637] (ICC, Human)
• IT Lee, T Nakayama, CT Wu, Y Goltsev, S Jiang, PA Gall, CK Liao, LC Shih, CM Schurch, DR McIlwain, P Chu, NA Borchard, D Zarabanda, SS Dholakia, A Yang, D Kim, T Kanie, CD Lin, MH Tsai, KM Phillips, R Kim, JB Overdevest, MA Tyler, CH Yan, CF Lin, YT Lin, DT Bau, GJ Tsay, ZM Patel, YA Tsou, CJ Tai, TH Yeh, PH Hwang, GP Nolan, JV Nayak, PK Jackson Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers medRxiv, 2020;0(0):. 2020 [PMID: 32511516] (IHC, Human)
• Y Han, L Yang, X Duan, F Duan, BE Nilsson-Pa, TM Yaron, P Wang, X Tang, T Zhang, Z Zhao, Y Bram, D Redmond, S Houghton, D Nguyen, D Xu, X Wang, S Uhl, Y Huang, JL Johnson, J Xiang, H Wang, FC Pan, LC Cantley, BR tenOever, DD Ho, T Evans, RE Schwartz, HJ Chen, S Chen Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids bioRxiv, 2020;0(0):. 2020 [PMID: 32511403] (ICC, Human)
• J Collin, R Queen, D Zerti, B Dorgau, M Georgiou, I Djidrovski, R Hussain, JM Coxhead, A Joseph, P Rooney, S Lisgo, F Figueiredo, L Armstrong, M Lako Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface Ocul Surf, 2020;0(0):. 2020 [PMID: 32502616] (Western Blot, Human)
• J Zhou, C Li, X Liu, MC Chiu, X Zhao, D Wang, Y Wei, A Lee, AJ Zhang, H Chu, JP Cai, CC Yip, IH Chan, KK Wong, OT Tsang, KH Chan, JF Chan, KK To, H Chen, KY Yuen Infection of bat and human intestinal organoids by SARS-CoV-2 Nat. Med., 2020;0(0):. 2020 [PMID: 32405028] (ICC, Bat)
• KHD Crawford, R Eguia, AS Dingens, AN Loes, KD Malone, CR Wolf, HY Chu, MA Tortorici, D Veesler, M Murphy, D Pettie, NP King, AB Balazs, JD Bloom Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays Viruses, 2020;12(5):. 2020 [PMID: 32384820] (Flow Cytometry, Human)
• M Yang, X Ma, X Xuan, H Deng, Q Chen, L Yuan Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System Front Pharmacol, 2020;11(0):432. 2020 [PMID: 32322207] (Western Blot, Human)
• Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun, 0;11(1):1620. 0 [PMID: 32221306] (Flow Cytometry, Human)
• Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(0):1-10. 2020 [PMID: 32142651] (Neutralization, Primate - Cercopithecus aethiops (African Green Monkey))
• D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 2019;9(1):9193. 2019 [PMID: 31235714] (Rhesus Macaca (Macaque))
• E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 2017;7(0):350-365. 2017 [PMID: 28624211] (Western Blot, Human)
• Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 2016;0(0):1663-9812. 2016 [PMID: 27313531] (Western Blot, Mouse)
• Aleksandra Milewska HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016;11(6):e0156552. 2016 [PMID: 27249425] (IHC, Primate - Macaca mulatta (Rhesus Macaque))
• Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 2016;23(1):14. 2016 [PMID: 26801988] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 2014;9(1):e85958. 2014 [PMID: 24454948] (Western Blot, Mouse)
• Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013;10(0):359. 2013 [PMID: 24364985] (IHC, Human)
• Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Flow Cytometry, Human)
• Ge , Xing-Yi, Li , Jia-Lu, Yang , Xing-Lou, Chmura , Aleksei, Zhu , Guangjia, Epstein , Jonathan, Mazet , Jonna K, Hu , Ben, Zhang , Wei, Peng , Cheng, Zhang , Yu-Ji, Luo , Chu-Ming, Tan , Bing, Wang , Ning, Zhu , Yan, Crameri , Gary, Zhang , Shu-Yi, Wang , Lin-Fa, Daszak , Peter, Shi , Zheng-Li Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature, 2013;503(7477):535-8. 2013 [PMID: 24172901] (IHC, Bat)
• Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 2012;7(4):e35876. 2012 [PMID: 22558251] (IHC-P, Human)
• Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010;285(53):41935-46. 2010 [PMID: 20966072] (Western Blot, Mouse)
• Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology, 2008;381(2):215-21. 2008 [PMID: 18814896] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 2008;52(9):750-4. 2008 [PMID: 18718423] (Neutralization, Human)
• Lu Y, Liu DX, Tam JP Lipid rafts are involved in SARS-CoV entry into Vero E6 cells. Biochem. Biophys. Res. Commun., 2008;369(2):344-9. 2008 [PMID: 18279660] (Flow Cytometry, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, Zhang SY, Wang LF, Deng H, Shi Z Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin. J. Virol., 2007;82(4):1899-907. 2007 [PMID: 18077725] (Western Blot, Human)
• Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted. J. Virol., 2007;81(16):8722-9. 2007 [PMID: 17522231] (Western Blot, Primate - Macaca mulatta (Rhesus Macaque))
• Rockx B, Sheahan T, Donaldson E, Harkema J, Sims A, Heise M, Pickles R, Cameron M, Kelvin D, Baric R Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice. J. Virol., 2007;81(14):7410-23. 2007 [PMID: 17507479] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• de Lang A, Osterhaus AD, Haagmans BL Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 2006;353(2):474-81. 2006 [PMID: 16860835] (ICC, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Tseng CT, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J. Virol., 2005;79(15):9470-9. 2005 [PMID: 16014910] (ICC, Human)
• Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor. Biochem. Biophys. Res. Commun., 2004;319(4):1216-21. 2004 [PMID: 15194496] (Flow Cytometry, Human)

Review for ACE-2 Antibody (AF933) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23232719
• Species: Human

Blocking

• Blocking Details: See PMID 23232719

Details

• Detection Notes: See PMID 23232719

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23232719.

Bioinformatics

• Gene Symbol: ACE2
• Uniprot:
  • Q9BYF1()