ACE-2 Antibody [Unconjugated]


Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen more
HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was stained with (A) Goat Anti-Human ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) or (B) Goat IgG control antibody (Catalog more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue more
ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue more

Product Details

Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, B/N

ACE-2 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human ACE-2
Accession # Q9BYF1
Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 1 Review rated 5
AF933 in the following applications:

Read Publications using
AF933 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

  • ACE2
  • ACE-2
  • ACEH
  • ACEHangiotensin I converting enzyme 2
  • ACE-related carboxypeptidase
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin-converting enzyme 2
  • Angiotensin-converting enzyme homolog
  • DKFZp434A014
  • EC 3.4.17
  • EC
  • Metalloprotease MPROT15


ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower,  M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Li, W. et al. (2003) Nature 426:450.
  5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ha
Applications: WB, ELISA, ICC/IF, RNAi
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Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
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Applications: WB, Flow, ICC/IF, PEP-ELISA, IHC-FrFl

Publications for ACE-2 Antibody (AF933)(26)

We have publications tested in 7 confirmed species: Human, Mouse, Bat, Primate - Cercopithecus aethiops (African Green Monkey), Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Rhesus Macaca (Macaque).

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-P, Neutralization, Western Blot.

Filter By Application
Flow Cytometry
Western Blot
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Filter By Species
Primate - Cercopithecus aethiops (African Green Monkey)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca mulatta (Rhesus Macaque)
Rhesus Macaca (Macaque)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF933 Applications Species
R Rathnasing, S Strohmeier, F Amanat, VL Gillespie, F Krammer, A García-Sas, L Coughlan, M Schotsaert, M Uccellini Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection bioRxiv, 2020;0(0):. 2020 [PMID: 32676603] (Flow Cytometry, Human) Flow Cytometry Human
J Zhou, C Li, X Liu, MC Chiu, X Zhao, D Wang, Y Wei, A Lee, AJ Zhang, H Chu, JP Cai, CC Yip, IH Chan, KK Wong, OT Tsang, KH Chan, JF Chan, KK To, H Chen, KY Yuen Infection of bat and human intestinal organoids by SARS-CoV-2 Nat. Med., 2020;0(0):. 2020 [PMID: 32405028] (ICC, Bat) ICC Bat
KHD Crawford, R Eguia, AS Dingens, AN Loes, KD Malone, CR Wolf, HY Chu, MA Tortorici, D Veesler, M Murphy, D Pettie, NP King, AB Balazs, JD Bloom Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays Viruses, 2020;12(5):. 2020 [PMID: 32384820] (Flow Cytometry, Human) Flow Cytometry Human
M Yang, X Ma, X Xuan, H Deng, Q Chen, L Yuan Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System Front Pharmacol, 2020;11(0):432. 2020 [PMID: 32322207] (Western Blot, Human) Western Blot Human
Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun, 0;11(1):1620. 0 [PMID: 32221306] (Flow Cytometry, Human) Flow Cytometry Human
Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(0):1-10. 2020 [PMID: 32142651] (Neutralization, Primate - Cercopithecus aethiops (African Green Monkey)) Neutralization Primate - Cercopithecus aethiops (African Green Monkey)
D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 2019;9(1):9193. 2019 [PMID: 31235714] (Rhesus Macaca (Macaque)) Rhesus Macaca (Macaque)
E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 2017;7(0):350-365. 2017 [PMID: 28624211] (Western Blot, Human) Western Blot Human
Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 2016;0(0):1663-9812. 2016 [PMID: 27313531] (Western Blot, Mouse) Western Blot Mouse
Aleksandra Milewska HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016;11(6):e0156552. 2016 [PMID: 27249425] (IHC, Primate - Macaca mulatta (Rhesus Macaque)) IHC Primate - Macaca mulatta (Rhesus Macaque)
Show All 26 Publications.

Review for ACE-2 Antibody (AF933) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF933:
Filter by Applications
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All Species
Images Ratings Applications Species Date Details
reviewed by:
IF Human 01/12/2015


Sample TestedSee PMID 23232719


Blocking DetailsSee PMID 23232719


Detection NotesSee PMID 23232719


CommentsIndependent review from antibodyresource.comPublished in PMID 23232719.

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Blogs on ACE-2.

COVID-19 and the Cardiovascular System: Observed complications and potential mechanisms
By Victoria OsinskiThe outbreak of COVID-19 resulting from the transmission of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in many cases of illness typically manifesting in mi...  Read full blog post.

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Tiny Antibodies (VHHs) from Llama Neutralize Respiratory Coronaviruses
By Jamshed Arslan, Pharm. D., PhD. VHH Single Domain Antibodies vs Conventional AntibodiesThe immune system protects living organisms against harmful substances. B cells ward off infections by producing antibodies t...  Read full blog post.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Application: IF
Species: Human


Gene Symbol ACE2