ACE-2 Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ACE‑2 was detected in immersion fixed frozen sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in eputhelial cells in bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, Simple Western, Flow, IHC, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ACE-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
4. Li, W. et al. (2003) Nature 426:450.
5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (AF933)(114)

Publications using AF933

• Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA; Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface The Journal of biological chemistry 2023-05-13 [PMID: 37187291] (Flow Cytometry, Human)
• Barreto, EA;Cruz, AS;Veras, FP;Martins, R;Bernardelli, RS;Paiva, IM;Lima, TM;Singh, Y;Guimar�es, RC;Damasceno, S;Pereira, N;Alves, JM;Gon�alves, TT;Forato, J;Muraro, SP;Souza, GF;Batah, SS;Proenca-Modena, JL;Mori, MA;Cunha, FQ;Louzada-Junior, P;Cunha, TM;Nakaya, HI;Fabro, A;de Oliveira, RDR;Arruda, E;R�a, R;R�a Neto, �;Fernandes da Silva, MM;Leiria, LO; COVID-19-related hyperglycemia is associated with infection of hepatocytes and stimulation of gluconeogenesis Proceedings of the National Academy of Sciences of the United States of America 2023-05-23 [PMID: 37186819] (Neutralization, Human)
• M Ulinici, A Sulji?, M Poggianell, R Milan Bono, K Resman Rus, A Paraschiv, AM Bonetti, M Todiras, A Corlateanu, S Groppa, E Ceban, M Petrovec, A Marcello Characterisation of the Antibody Response in Sinopharm (BBIBP-CorV) Recipients and COVID-19 Convalescent Sera from the Republic of Moldova Vaccines, 2023-03-13;11(3):. 2023-03-13 [PMID: 36992221] (FLOW, Human)
• AT Tang, DW Buchholz, KM Szigety, B Imbiakha, S Gao, M Frankfurte, M Wang, J Yang, P Hewins, P Mericko-Is, NA Leu, S Sterling, IA Monreal, J Sahler, A August, X Zhu, KA Jurado, M Xu, EE Morrisey, SE Millar, HC Aguilar, ML Kahn Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection PloS Biology, 2023-02-06;21(2):e3001989. 2023-02-06 [PMID: 36745682] (IHC, Western Blot, Mouse)
• N Tailor, BM Warner, BD Griffin, K Tierney, E Moffat, K Frost, R Vendramell, A Leung, M Willman, SP Thomas, Y Pei, SA Booth, C Embury-Hya, SK Wootton, D Kobasa Generation and Characterization of a SARS-CoV-2-Susceptible Mouse Model Using Adeno-Associated Virus (AAV6.2FF)-Mediated Respiratory Delivery of the Human ACE2 Gene Viruses, 2022-12-28;15(1):. 2022-12-28 [PMID: 36680125] (Western Blot, Human)
• Y Kato, K Nishiyama, J Man Lee, Y Ibuki, Y Imai, T Noda, N Kamiya, T Kusakabe, Y Kanda, M Nishida TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation International Journal of Molecular Sciences, 2022-12-21;24(1):. 2022-12-21 [PMID: 36613540] (Western Blot, Mouse)
• A Miluzio, A Cuomo, C Cordiglier, L Donnici, E Pesce, M Bombaci, M Conti, A Fasciani, L Terraccian, L Manganaro, M Toccafondi, A Scagliola, S Oliveto, S Ricciardi, R Grifantini, R De Frances, S Abrignani, N Manfrini, S Biffo Mapping of functional SARS-CoV-2 receptors in human lungs establishes differences in variant binding and SLC1A5 as a viral entry modulator of hACE2 EBioMedicine, 2022-12-28;87(0):104390. 2022-12-28 [PMID: 36584595] (Co-Immunoprecipitation, Flow Cytometry, IHC, Western Blot, Human)
• X Guo, J Cao, JP Cai, J Wu, J Huang, P Asthana, SKK Wong, ZW Ye, S Gurung, Y Zhang, S Wang, Z Wang, X Ge, HY Kwan, A Lyu, KM Chan, N Wong, J Huang, Z Zhou, ZX Bian, S Yuan, HLX Wong Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2 Nature Communications, 2022-12-23;13(1):7907. 2022-12-23 [PMID: 36564389] (ICC, Human)
• DR Adney, J Lovaglio, JE Schulz, CK Yinda, VA Avanzato, E Haddock, JR Port, MG Holbrook, PW Hanley, G Saturday, D Scott, C Shaia, AM Nelson, JR Spengler, C Tansey, CM Cossaboom, NM Wendling, C Martens, J Easley, SW Yap, SN Seifert, VJ Munster Severe acute respiratory disease in American mink experimentally infected with SARS-CoV-2 JCI Insight, 2022-11-22;7(22):. 2022-11-22 [PMID: 36509288] (IHC, Mink)
• SB Biering, FT Gomes de S, LV Tjang, F Pahmeier, C Zhu, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, DM Fox, V Ortega, AM Näär, RS Baric, SA Stanley, HC Aguilar, JD Esko, CY Chiu, JE Pak, PR Beatty, E Harris SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling Nature Communications, 2022-12-09;13(1):7630. 2022-12-09 [PMID: 36494335] (Western Blot, Human)
• H Guo, A Li, TY Dong, J Su, YL Yao, Y Zhu, ZL Shi, M Letko ACE2-Independent Bat Sarbecovirus Entry and Replication in Human and Bat Cells MBio, 2022-11-21;0(0):e0256622. 2022-11-21 [PMID: 36409074] (Neutralization, Bat, Human)
• JV Harte, SL Wakerlin, AJ Lindsay, JV McCarthy, C Coleman-Va Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2 Viruses, 2022-09-21;14(10):. 2022-09-21 [PMID: 36298651]
• J Song, RD Chow, M Pena-Herna, L Zhang, SA Loeb, EY So, OD Liang, P Ren, S Chen, CB Wilen, S Lee LRRC15 inhibits SARS-CoV-2 cellular entry in trans PloS Biology, 2022-10-13;20(10):e3001805. 2022-10-13 [PMID: 36228039] (Flow Cytometry, Human)
• K Shilagardi, ED Spear, R Abraham, DE Griffin, S Michaelis The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia Formation MBio, 2022-10-05;0(0):e0254322. 2022-10-05 [PMID: 36197088] (Western Blot, Human)
• TD Saccon, F Mousovich-, RG Ludwig, VC Carregari, AB Dos Anjos, ASC Dos Passos, MC Martini, PP Barbosa, GF de Souza, SP Muraro, J Forato, MR Amorim, RE Marques, FP Veras, E Barreto, TT Gonçalves, IM Paiva, NPB Fazolini, CMK Onodera, RB Martins Ju, PHC de Araújo, SS Batah, RMM Viana, DM de Melo, AT Fabro, E Arruda, F Queiroz Cu, TM Cunha, MAM Pretti, BJ Smith, H Marques-So, TL Knittel, GP Ruiz, GS Profeta, TCM Fontes-Cal, M Boroni, MAR Vinolo, AS Farias, PMM Moraes-Vie, JMA Bizzacchi, T Teesalu, FDM Chaim, E Cazzo, EA Chaim, JL Proença-Mó, D Martins-de, MK Osako, LO Leiria, MA Mori SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner Nature Communications, 2022-09-29;13(1):5722. 2022-09-29 [PMID: 36175400] (ICC, African Green Monkey)
• N Brasu, I Elia, V Russo, G Montacchie, SA Stabile, C De Intinis, F Fesi, K Gizzi, M Macagno, M Montone, B Mussolin, A Grifoni, S Faravelli, S Marchese, F Forneris, R De Frances, A Sette, V Barnaba, A Sottile, A Sapino, L Pace Memory CD8+ T cell diversity and B cell responses correlate with protection against SARS-CoV-2 following mRNA vaccination Nature Immunology, 2022-09-22;0(0):. 2022-09-22 [PMID: 36138186] (Flow Cytometry, Human)
• M Montano, AR Victor, DK Griffin, T Duong, N Bolduc, A Farmer, V Garg, AK Hadjantona, A Coates, FL Barnes, CG Zouves, WC Greene, M Viotti SARS-CoV-2 can infect human embryos Scientific Reports, 2022-09-14;12(1):15451. 2022-09-14 [PMID: 36104397] (Control, ICC, Human)
• Y Yao, K Subedi, T Liu, N Khalasawi, CD Pretto-Ker, JW Wotring, J Wang, C Yin, A Jiang, C Fu, P Dimitrion, J Li, J Veenstra, Q Yi, K McKinnon, JE McKinnon, JZ Sexton, L Zhou, QS Mi Surface translocation of ACE2 and TMPRSS2 upon TLR4/7/8 activation is required for SARS-CoV-2 infection in circulating monocytes Cell Discovery, 2022-09-09;8(1):89. 2022-09-09 [PMID: 36085197] (Western Blot, Human)
• J Hou, Y Wei, J Zou, R Jaffery, S Liang, C Zheng, K Chen, PY Shi, Y Chen, X Xie, W Peng Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection Research square, 2022-08-15;0(0):. 2022-08-15 [PMID: 36032971] (Western Blot, Human)
• B Meng, R Datir, J Choi, CITIID-NIH, JR Bradley, KGC Smith, JH Lee, RK Gupta SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicity Cell Reports, 2022-08-03;40(7):111220. 2022-08-03 [PMID: 35963244] (Neutralization, Human)
• P Su, D Zhai, AHC Wong, F Liu Development of a novel peptide to prevent entry of SARS-CoV-2 into lung and olfactory bulb cells of hACE2 expressing mice Molecular Brain, 2022-08-09;15(1):71. 2022-08-09 [PMID: 35945596] (Co-Immunoprecipitation, IHC, Western Blot, Human, Mouse)
• Y Zhu, KY Chew, M Wu, AC Karawita, G McCallum, LE Steele, A Yamamoto, LI Labzin, T Yarlagadda, AA Khromykh, X Wang, JDJ Sng, CJ Stocks, Y Xia, TR Kollmann, D Martino, M Joensuu, FA Meunier, G Balistreri, H Bielefeldt, AC Bowen, A Kicic, PD Sly, KM Spann, KR Short Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells PloS Biology, 2022-08-01;20(8):e3001728. 2022-08-01 [PMID: 35913989] (ICC, Western Blot, Human)
• CW Chang, KM Parsi, M Somasundar, E Vanderleed, P Liu, J Cruz, A Cousineau, RW Finberg, EA Kurt-Jones A Newly Engineered A549 Cell Line Expressing ACE2 and TMPRSS2 Is Highly Permissive to SARS-CoV-2, Including the Delta and Omicron Variants Viruses, 2022-06-23;14(7):. 2022-06-23 [PMID: 35891350] (Flow Cytometry, Human)
• H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1 Mucosal Immunology, 2022-07-15;0(0):. 2022-07-15 [PMID: 35840680] (IHC, Human)
• M Ramal-Sanc, C Castellini, C Cimini, A Taraschi, L Valbonetti, A Barbonetti, N Bernabò, B Barboni ACE2 Receptor and Its Isoform Short-ACE2 Are Expressed on Human Spermatozoa International Journal of Molecular Sciences, 2022-03-28;23(7):. 2022-03-28 [PMID: 35409054] (Flow Cytometry, Human)
• P Spitalieri, F Centofanti, M Murdocca, MG Scioli, A Latini, S Di Cesare, G Citro, A Rossi, A Orlandi, S Miersch, SS Sidhu, PP Pandolfi, A Botta, F Sangiuolo, G Novelli Two Different Therapeutic Approaches for SARS-CoV-2 in hiPSCs-Derived Lung Organoids Cells, 2022-04-05;11(7):. 2022-04-05 [PMID: 35406799] (IHC, Human)
• C Junqueira, Â Crespo, S Ranjbar, LB de Lacerda, M Lewandrows, J Ingber, B Parry, S Ravid, S Clark, MR Schrimpf, F Ho, C Beakes, J Margolin, N Russell, K Kays, J Boucau, U Das Adhika, SM Vora, V Leger, L Gehrke, L Henderson, E Janssen, D Kwon, C Sander, J Abraham, MB Goldberg, H Wu, G Mehta, S Bell, AE Goldfeld, MR Filbin, J Lieberman FcgammaR-mediated SARS-CoV-2 infection of monocytes activates inflammation Nature, 2022-04-06;0(0):. 2022-04-06 [PMID: 35385861] (Flow Cytometry, Neutralization, Human)
• L Casciola-R, DR Thiemann, F Andrade, MI Trejo-Zamb, EK Leonard, JB Spangler, NE Skinner, J Bailey, S Yegnasubra, R Wang, AM Vaghasia, A Gupta, AL Cox, SC Ray, RM Linville, Z Guo, PC Searson, CE Machamer, S Desiderio, LM Sauer, O Laeyendeck, BT Garibaldi, L Gao, M Damarla, PM Hassoun, JE Hooper, CA Mecoli, L Christophe, L Gutierrez-, Q Yang, D Hines, WA Clarke, RE Rothman, A Pekosz, KZ Fenstermac, Z Wang, SL Zeger, A Rosen IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function JCI Insight, 2022-05-09;7(9):. 2022-05-09 [PMID: 35349483] (IHC, Human)
• Y Menuchin-L, A Schreiber, A Lecanda, A Mecate-Zam, L Brunotte, OE Psathaki, S Ludwig, T Rauen, HR Schöler SARS-CoV-2 infects and replicates in photoreceptor and retinal ganglion cells of human retinal organoids Stem Cell Reports, 2022-03-24;0(0):. 2022-03-24 [PMID: 35334213] (Neutralization, Human)
• X Wang, J Luo, Z Wen, L Shuai, C Wang, G Zhong, X He, H Cao, R Liu, J Ge, R Hua, Z Sun, X Wang, J Wang, Z Bu Diltiazem inhibits SARS-CoV-2 cell attachment and internalization and decreases the viral infection in mouse lung PloS Pathogens, 2022-02-17;18(2):e1010343. 2022-02-17 [PMID: 35176124] (Flow Cytometry, Human)
• S Krasemann, U Haferkamp, S Pfefferle, MS Woo, F Heinrich, M Schweizer, A Appelt-Men, A Cubukova, J Barenberg, J Leu, K Hartmann, E Thies, JL Littau, D Sepulveda-, L Zhang, K Ton, Y Liang, J Matschke, F Ricklefs, T Sauvigny, J Sperhake, A Fitzek, A Gerhartl, A Brachner, N Geiger, EM König, J Bodem, S Franzenbur, A Franke, S Moese, FJ Müller, G Geisslinge, C Claussen, A Kannt, A Zaliani, P Gribbon, B Ondruschka, W Neuhaus, MA Friese, M Glatzel, O Pless The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2 Stem Cell Reports, 2022-01-20;0(0):. 2022-01-20 [PMID: 35063125] (Neutralization, Human)
• L El-Shennaw, AD Hoffmann, NK Dashzeveg, KM McAndrews, PJ Mehl, D Cornish, Z Yu, VL Tokars, V Nicolaescu, A Tomatsidou, C Mao, CJ Felicelli, CF Tsai, C Ostiguin, Y Jia, L Li, K Furlong, J Wysocki, X Luo, CF Ruivo, D Batlle, TJ Hope, Y Shen, YK Chae, H Zhang, VS LeBleu, T Shi, S Swaminatha, Y Luo, D Missiakas, GC Randall, AR Demonbreun, MG Ison, R Kalluri, D Fang, H Liu Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2 Nature Communications, 2022-01-20;13(1):405. 2022-01-20 [PMID: 35058437] (Western Blot, Human)
• J Grau-Expós, D Perea, M Suppi, N Massana, A Vergara, MJ Soler, B Trinite, J Blanco, J García-Pér, J Alcamí, A Serrano-Mo, J Rosado, V Falcó, M Genescà, MJ Buzon Evaluation of SARS-CoV-2 entry, inflammation and new therapeutics in human lung tissue cells PloS Pathogens, 2022-01-13;18(1):e1010171. 2022-01-13 [PMID: 35025963] (ICC, IHC, Human)
• J Shi, X Jin, Y Ding, X Liu, A Shen, Y Wu, M Peng, C Shen Receptor-Binding Domain Proteins of SARS-CoV-2 Variants Elicited Robust Antibody Responses Cross-Reacting with Wild-Type and Mutant Viruses in Mice Vaccines, 2021-11-24;9(12):. 2021-11-24 [PMID: 34960131] (ELISA Detection, Human)
• SB Biering, FTG de Sousa, LV Tjang, F Pahmeier, R Ruan, SF Blanc, TS Patel, CM Worthingto, DR Glasner, B Castillo-R, V Servellita, NTN Lo, MP Wong, CM Warnes, DR Sandoval, TM Clausen, YA Santos, V Ortega, HC Aguilar, JD Esko, CY Chui, JE Pak, PR Beatty, E Harris SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-beta signaling bioRxiv : the preprint server for biology, 2021-12-13;0(0):. 2021-12-13 [PMID: 34931188] (Western Blot, Human)
• J Wang, G Yang, X Wang, Z Wen, L Shuai, J Luo, C Wang, Z Sun, R Liu, J Ge, X He, R Hua, X Wang, X Yang, W Chen, G Zhong, Z Bu SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells Cell Discovery, 2021-12-14;7(1):119. 2021-12-14 [PMID: 34903715] (Flow Cytometry, Neutralization, Human, Primate - C. aethiops)
• L Helms, S Marchiano, IB Stanaway, TY Hsiang, BA Juliar, S Saini, YT Zhao, A Khanna, R Menon, F Alakwaa, C Mikacenic, ED Morrell, MM Wurfel, M Kretzler, JL Harder, CE Murry, J Himmelfarb, H Ruohola-Ba, PK Bhatraju, M Gale, BS Freedman Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations JCI Insight, 2021-12-22;0(0):. 2021-12-22 [PMID: 34767537] (IHC, Human)
• L Nguyen, KA McCord, DT Bui, KM Bouwman, EN Kitova, M Elaish, D Kumawat, GC Daskhan, I Tomris, L Han, P Chopra, TJ Yang, SD Willows, AL Mason, LK Mahal, TL Lowary, LJ West, SD Hsu, T Hobman, SM Tompkins, GJ Boons, RP de Vries, MS Macauley, JS Klassen Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2 Nature Chemical Biology, 2021-11-09;0(0):. 2021-11-09 [PMID: 34754101] (Flow Cytometry, Human)
• X Duan, X Tang, MS Nair, T Zhang, Y Qiu, W Zhang, P Wang, Y Huang, J Xiang, H Wang, RE Schwartz, DD Ho, T Evans, S Chen An airway organoid-based screen identifies a role for the HIF1alpha-glycolysis axis in SARS-CoV-2 infection Cell Reports, 2021-10-15;37(6):109920. 2021-10-15 [PMID: 34731648] (IHC, Human)
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Review for ACE-2 Antibody (AF933) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: cynthia morimoto IF Human 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23232719
• Species: Human

Bioinformatics

• Gene Symbol: ACE2
• Uniprot:
  • Q9BYF1()