ACE-2 Antibody [Unconjugated]

Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was stained with (A) Goat Anti-Human ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) or (B) Goat IgG control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, Simple Western, Flow, IHC, IP, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ACE-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Blockade of Receptor-ligand Interaction

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Unconjugated]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
4. Li, W. et al. (2003) Nature 426:450.
5. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (AF933)(26)

Publications using AF933

• R Rathnasing, S Strohmeier, F Amanat, VL Gillespie, F Krammer, A García-Sas, L Coughlan, M Schotsaert, M Uccellini Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection bioRxiv, 2020;0(0):. 2020 [PMID: 32676603] (Flow Cytometry, Human)
• J Zhou, C Li, X Liu, MC Chiu, X Zhao, D Wang, Y Wei, A Lee, AJ Zhang, H Chu, JP Cai, CC Yip, IH Chan, KK Wong, OT Tsang, KH Chan, JF Chan, KK To, H Chen, KY Yuen Infection of bat and human intestinal organoids by SARS-CoV-2 Nat. Med., 2020;0(0):. 2020 [PMID: 32405028] (ICC, Bat)
• KHD Crawford, R Eguia, AS Dingens, AN Loes, KD Malone, CR Wolf, HY Chu, MA Tortorici, D Veesler, M Murphy, D Pettie, NP King, AB Balazs, JD Bloom Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays Viruses, 2020;12(5):. 2020 [PMID: 32384820] (Flow Cytometry, Human)
• M Yang, X Ma, X Xuan, H Deng, Q Chen, L Yuan Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System Front Pharmacol, 2020;11(0):432. 2020 [PMID: 32322207] (Western Blot, Human)
• Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen X, Chen J, Hu K, Jin Q, Wang J, Qian Z Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun, 0;11(1):1620. 0 [PMID: 32221306] (Flow Cytometry, Human)
• Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S, Schiergens T, Herrler G, Wu N, Nitsche A, Muller M, Drosten C, Pohlmann S SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(0):1-10. 2020 [PMID: 32142651] (Neutralization, Primate - Cercopithecus aethiops (African Green Monkey))
• D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa Protective effects of the Francisella tularensis δpdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 2019;9(1):9193. 2019 [PMID: 31235714] (Rhesus Macaca (Macaque))
• E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 2017;7(0):350-365. 2017 [PMID: 28624211] (Western Blot, Human)
• Fengxia Xiao, Joseph Zimpelman, Dylan Burger, Christopher Kennedy, Richard L Hébert, Kevin D Burns Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 2016;0(0):1663-9812. 2016 [PMID: 27313531] (Western Blot, Mouse)
• Aleksandra Milewska HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 2016;11(6):e0156552. 2016 [PMID: 27249425] (IHC, Primate - Macaca mulatta (Rhesus Macaque))
• Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 2016;23(1):14. 2016 [PMID: 26801988] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 2014;9(1):e85958. 2014 [PMID: 24454948] (Western Blot, Mouse)
• Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013;10(0):359. 2013 [PMID: 24364985] (IHC, Human)
• Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 2014;88(2):1293-307. 2014 [PMID: 24227843] (Flow Cytometry, Human)
• Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature, 2013;503(7477):535-8. 2013 [PMID: 24172901] (IHC, Bat)
• Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 2012;7(4):e35876. 2012 [PMID: 22558251] (IHC-P, Human)
• Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010;285(53):41935-46. 2010 [PMID: 20966072] (Western Blot, Mouse)
• Glende J, Schwegmann-Wessels C, Al-Falah M, Pfefferle S, Qu X, Deng H, Drosten C, Naim HY, Herrler G Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology, 2008;381(2):215-21. 2008 [PMID: 18814896] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Epelman S, Tang WH, Chen SY, Van Lente F, Francis GS, Sen S Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 2008;52(9):750-4. 2008 [PMID: 18718423] (Neutralization, Human)
• Lu Y, Liu DX, Tam JP Lipid rafts are involved in SARS-CoV entry into Vero E6 cells. Biochem. Biophys. Res. Commun., 2008;369(2):344-9. 2008 [PMID: 18279660] (Flow Cytometry, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Ren W, Qu X, Li W, Han Z, Yu M, Zhou P, Zhang SY, Wang LF, Deng H, Shi Z Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin. J. Virol., 2007;82(4):1899-907. 2007 [PMID: 18077725] (Western Blot, Human)
• Inoue Y, Tanaka N, Tanaka Y, Inoue S, Morita K, Zhuang M, Hattori T, Sugamura K Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted. J. Virol., 2007;81(16):8722-9. 2007 [PMID: 17522231] (Western Blot, Primate - Macaca mulatta (Rhesus Macaque))
• Rockx B, Sheahan T, Donaldson E, Harkema J, Sims A, Heise M, Pickles R, Cameron M, Kelvin D, Baric R Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice. J. Virol., 2007;81(14):7410-23. 2007 [PMID: 17507479] (Neutralization, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• de Lang A, Osterhaus AD, Haagmans BL Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 2006;353(2):474-81. 2006 [PMID: 16860835] (ICC, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Tseng CT, Tseng J, Perrone L, Worthy M, Popov V, Peters CJ Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J. Virol., 2005;79(15):9470-9. 2005 [PMID: 16014910] (ICC, Human)
• Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor. Biochem. Biophys. Res. Commun., 2004;319(4):1216-21. 2004 [PMID: 15194496] (Flow Cytometry, Human)

Review for ACE-2 Antibody (AF933) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IF Human 01/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23232719
• Species: Human

Blocking

• Blocking Details: See PMID 23232719

Details

• Detection Notes: See PMID 23232719

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23232719.

Bioinformatics

• Gene Symbol: ACE2
• Uniprot:
  • Q9BYF1()