Recombinant Mouse Slit2 (aa 26-900) Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Slit2 (aa 26-900) Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Recombinant Mouse Slit2 immobilized at 3-12 μg/mL, is able to significantly induce neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Slit2 protein
Gln26-Gln900, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained; Gln26 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
Slit2
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
99.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-115 kDa, reducing conditions
Publications
Read Publications using
5444-SL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Slit2 (aa 26-900) Protein, CF

  • FLJ14420
  • SLIL3
  • slit (Drosophila) homolog 2
  • slit homolog 2 (Drosophila)
  • slit homolog 2 protein
  • Slit2
  • Slit-2SLIL3

Background

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including nine EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature mouse Slit2 shares 96% and 98% amino acid sequence identity with the human and rat orthologs, respectively.

Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican-1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to
Glypican-1 and promotes motor axon migration (5). Outside the nervous system, Slit2 plays a role in a wide range of biological processes including cell adhesion and migration, tumor progression and metastasis, angiogenesis, lymphangiogenesis, HIV-1 replication, platelet function and thrombus formation, and stem cell senescence (1, 8, 17-30).

  1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
  2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
  3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
  4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
  5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  6. Nguyen Ba-Charvet, K.T. et al. (2001) Mol. Cell. Neurosci. 17:1048.
  7. Hagino, S. et al. (2003) Glia 42:130.
  8. Chen, B. et al. (2004) J. Immunol. 173:5914.
  9. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  10. Erskine, L. et al. (2000) J. Neurosci. 20:4975.
  11. Shu, T. et al. (2003) J. Neurosci. 23:8176.
  12. Kim, M. et al. (2014) Neural Dev. 9:17.
  13. Wang, K.H. et al. (1999) Cell 96:771.
  14. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
  15. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
  16. Jaworski, A. et al. (2012) Nat. Neurosci. 15:367.
  17. Guan, H. et al. (2003) J. Immunol. 171:6519.
  18. Liu, D. et al. (2006) Circ. Res. 98:480.
  19. Prasad, A. et al. (2007) J. Leukoc. Biol. 82:465.
  20. Tole, S. et al. (2009) J. Leukoc. Biol. 86:1403.
  21. Kim, H.K. et al. (2008) Neoplasia 10:1411.
  22. Prasad, A. et al. (2008) J. Biol. Chem. 283:26624.
  23. Tseng, R.C. et al. (2010) Cancer Res. 70:543.
  24. Alajez, N.M. et al. (2011) Cancer Res. 71:2381.
  25. Zhang, Q.Q. et al. (2015) Oncotarget 6:3123.
  26. Dunaway, C.M. et al. (2011) Mol. Cell. Biol. 31:404.
  27. Yang, X.M. et al. (2010) Biochem. Biophys. Res. Commun. 396:571.
  28. Anand, A.R. et al. (2011) AIDS 25:2105.
  29. Patel, S. et al. (2012) Circulation 126:1385.
  30. Harburg, G. et al. (2014) Stem Cell Reports 3:385.

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Publications for Slit2 (5444-SL)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 5 applications: Adhesion Assay, Bioassay, Cell Culture, In Vivo, Proximity Ligation Assay.


Filter By Application
Adhesion Assay
(1)
Bioassay
(6)
Cell Culture
(1)
In Vivo
(3)
Proximity Ligation Assay
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(5)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 5444-SL Applications Species
G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019;10(1):2350. 2019 [PMID: 31138815] (Cell Culture, Human) Cell Culture Human
D Pilling, LE Chinea, KM Consalvo, RH Gomer Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils J. Immunol., 2018;0(0):. 2018 [PMID: 30510066] (Bioassay, Mouse) Bioassay Mouse
S Escot, D Willnow, H Naumann, S Di Frances, FM Spagnoli Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors Nat Commun, 2018;9(1):5082. 2018 [PMID: 30504829] (Bioassay, Human) Bioassay Human
N Aleksandro, I Gutsche, E Kandiah, SV Avilov, MV Petoukhov, E Seiradake, AA McCarthy Robo1 Forms a Compact Dimer-of-Dimers Assembly Structure, 2017;0(0):. 2017 [PMID: 29307485] (Proximity Ligation Assay, Human) Proximity Ligation Assay Human
B Erdogan, GM Cammarata, EJ Lee, BC Pratt, AF Francl, EL Rutherford, LA Lowery The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development Neural Dev, 2017;12(1):3. 2017 [PMID: 28202041] (Bioassay, Xenopus) Bioassay Xenopus
The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization Nat Commun, 2016;7(0):13517. 2016 [PMID: 27882935] (Bioassay, Human) Bioassay Human
Pilling D, Zheng Z, Vakil V, Gomer R Fibroblasts secrete Slit2 to inhibit fibrocyte differentiation and fibrosis. Proc Natl Acad Sci U S A, 2014;111(51):18291-6. 2014 [PMID: 25489114] (Bioassay, Human) Bioassay Human
Yeh M, Gonda Y, Mommersteeg M, Barber M, Ypsilanti A, Hanashima C, Parnavelas J, Andrews W Robo1 modulates proliferation and neurogenesis in the developing neocortex. J Neurosci, 2014;34(16):5717-31. 2014 [PMID: 24741061] (Bioassay, Mouse) Bioassay Mouse
Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis. Exp. Cell Res., 2012;318(4):371-8. 2012 [PMID: 22198087] (Adhesion Assay, Human) Adhesion Assay Human
Mulik S, Sharma S, Suryawanshi A Activation of endothelial roundabout receptor 4 reduces the severity of virus-induced keratitis. J. Immunol., 2011;186(12):7195-204. 2011 [PMID: 21572022] (In Vivo, Mouse) In Vivo Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol Slit2
Uniprot
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