Serpin A8/Angiotensinogen Antibody

Images

 
Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.
Immunocytochemistry/ Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Serpin A8/Angiotensinogen Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human Angiotensinogen protein (between residues 1-50) [UniProt P01019]
Localization
Secreted (Cytoplasm).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AGT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
  • Immunohistochemistry 1:40-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
This Angiotensinogen antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~53 kDa representing Angiotensinogen. In ICC/IF, cytoplasmic staining was observed in HepG2 cells. In IHC-P, secreted and cytoplasmic staining was observed in mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-30027 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin A8/Angiotensinogen Antibody

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

Angiotensin is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and functions also as a neurotransmitter. Angiotensin II is formed by catalytic action of the hydrolase called peptidyl dipeptidase A on C terminal oligopeptide (angiotensin I). In case of the cleavage of angiotensin I to the activated angiotensin II, it is called angiotensin converting enzyme (ACE); in case of the cleavage and inactivation of kinins, it is called kininase II (also called dipeptidyl carboxypeptidase I). The renin-angiotensin system plays an important role in the regulation of blood volume and blood pressure, stimulates antidiuretic hormone and aldosterone production, and the hypothalamus to activate the thirst reflex, but is crucial in the pathogenesis of hypertension patients due to blood vessels constrict, retention of fluid and raising blood pressure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A8/Angiotensinogen Antibody (NBP1-30027)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP1-30027 Applications Species
Kim YJ, Kim Yn, Kang B- et al. Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models J Tissue Eng Regen Med Sep 15 2016 [PMID: 27717202] (WB, Mouse) WB Mouse
Wu Y, Ma KL, Zhang Y et al. Lipid disorder and intrahepatic renin-angiotensin system activation synergistically contributes to non-alcoholic fatty liver disease. Liver Int. Mar 30 2016 [PMID: 27028410] (WB, Human) WB Human
Ishigami T, Kino T, Chen L et al. Identification of Bona Fide Alternative Renin Transcripts Expressed Along Cortical Tubules and Potential Roles in Promoting Insulin Resistance In Vivo Without Significant Plasma Renin Activity Elevation. Hypertension. 2014 Apr 28 [PMID: 24777979] (IHC-P, Mouse) IHC-P Mouse
Ahnstedt H, Cao L, Krause DN et al. Male-female differences in upregulation of vasoconstrictor responses in Human cerebral arteries. PLoS One 2013 Apr 29 [PMID: 23658641] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Shababi M, Habibi J, Ma L, Glascock JJ, Sowers JR, Lorson CL. Partial restoration of cardio-vascular defects in a rescued severe model of spinal muscular atrophy. J Mol Cell Cardiol;52(5):1074-82. 2012 May. [PMID: 22285962] (WB, Mouse) WB Mouse
Dimitrijevic I, Rissler P, Luts L, Edvinsson L. Reduced expression of angiotensin II and angiotensin receptor type 1 and type 2 in resistance arteries from nasal lesions in granulomatosis with polyangiitis (Wegener's granulomatosis). Scand J Rheumatol;40(6):448-52. 2011 Nov. [PMID: 21936613] (IHC, ICC/IF, Human) IHC, ICC/IF Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Serpin A8/Angiotensinogen Antibody (NBP1-30027). (Showing 1 - 2 of 2 FAQs).

  1. Can NBP1-30027 be used in brain sections from perfused rats? Do you have sample size to test it in brain sections?
    • This antibody is guaranteed for use on human, mouse and rat samples in Western blot, ICC and IHC on paraffin embedded tissues. We actually do have a sample size available for purchase.
  2. We have previously bought your NBP1-30027(anti-angiotensin II). Now it says Anti-Angiotensinogen as description when entering your web. Please confirm that it is still the same antibody.
    • I have checked our internal notes regarding NBP1-30027 and can see that we updated the common name of the protein in May of this year. This is still the same antibody that you bought previously, and all that has changed is the common name. The Uniprot reference for the protein, for your information, remains as follows: [UniProt# P01019].

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30027AF405
Alexa Fluor 488 Labeled NBP1-30027AF488
Alexa Fluor 647 Labeled NBP1-30027AF647
Alexa Fluor 700 Labeled NBP1-30027AF700
Biotin Labeled NBP1-30027B
DyLight 405 Labeled NBP1-30027V
DyLight 488 Labeled NBP1-30027G
DyLight 550 Labeled NBP1-30027R
DyLight 650 Labeled NBP1-30027C
FITC Labeled NBP1-30027F
HRP Labeled NBP1-30027H

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Bioinformatics

Gene Symbol AGT
Entrez
Uniprot