Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.
Immunocytochemistry/ Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in mouse kidney using DAB with hematoxylin counterstain.
This Angiotensinogen antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~53 kDa representing Angiotensinogen. In ICC/IF, cytoplasmic staining was observed in HepG2 cells. In IHC-P, secreted and cytoplasmic staining was observed in mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-30027 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Serpin A8/Angiotensinogen Antibody
alpha-1 antiproteinase, antitrypsin
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
serine (or cysteine) proteinase inhibitor
Angiotensin is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and functions also as a neurotransmitter. Angiotensin II is formed by catalytic action of the hydrolase called peptidyl dipeptidase A on C terminal oligopeptide (angiotensin I). In case of the cleavage of angiotensin I to the activated angiotensin II, it is called angiotensin converting enzyme (ACE); in case of the cleavage and inactivation of kinins, it is called kininase II (also called dipeptidyl carboxypeptidase I). The renin-angiotensin system plays an important role in the regulation of blood volume and blood pressure, stimulates antidiuretic hormone and aldosterone production, and the hypothalamus to activate the thirst reflex, but is crucial in the pathogenesis of hypertension patients due to blood vessels constrict, retention of fluid and raising blood pressure.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Serpin A8/Angiotensinogen Antibody (NBP1-30027). (Showing 1 - 2 of 2 FAQs).
Can NBP1-30027 be used in brain sections from perfused rats? Do you have sample size to test it in brain sections?
This antibody is guaranteed for use on human, mouse and rat samples in Western blot, ICC and IHC on paraffin embedded tissues. We actually do have a sample size available for purchase.
We have previously bought your NBP1-30027(anti-angiotensin II). Now it says Anti-Angiotensinogen as description when entering your web. Please confirm that it is still the same antibody.
I have checked our internal notes regarding NBP1-30027 and can see that we updated the common name of the protein in May of this year. This is still the same antibody that you bought previously, and all that has changed is the common name. The Uniprot reference for the protein, for your information, remains as follows: [UniProt# P01019].
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.