Recombinant Human Slit1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Slit1 Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well plates that had been pre-coated with 50 μL/well of Recombinant Human Slit1 at 2.5 ‑ 10 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Slit1 protein
Met1-Gln915, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Cys34
Protein/Peptide Type
Recombinant Proteins
Gene
SLIT1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
98.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-125 kDa, reducing conditions
Publications
Read Publications using
6514-SL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Slit1 Protein, CF

  • KIAA0813
  • MEGF4
  • MEGF4slit (Drosophila) homolog 1
  • MGC164811
  • Multiple EGF-like domains protein 4
  • Multiple epidermal growth factor-like domains protein 4
  • SLIL1
  • slit homolog 1 (Drosophila)
  • slit homolog 1 protein
  • Slit1
  • Slit-1slit1
  • SLIT3

Background

Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1, 2). Like other mammalian family members, the 1534 amino acid (aa), ~200 kDa human Slit1 contains a 33 aa signal sequence followed by 23 leucine-rich repeats (LRR, aa 34‑900) and 9 EGF-like sequences (aa 930‑1454) (2). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7 (aa 1166‑1339), and a C‑terminal cysteine-rich domain (cysteine knot; aa 1459‑1534) (2). Heparin sulfates are required for interaction of Robo with Slit LRR domains (2, 3). Within the LRR domains, human Slit1 shares 96% aa identity with mouse, rat, and bovine, 98% with equine and 91% with canine Slit1. Human Slits 1, 2 and 3 share 68-74% aa identity within the LRR domains. One potential isoform  of 1641 aa has 10 aa inserted after aa 338 within LRR9 and lacks aa 793‑816 within LRR20-21 (4). Two more potential isoforms of 1520 and 1461 aa diverge at the C‑terminus (aa 1453 and 1411, respectively); these isoforms lack the cysteine knot, which may mediate interaction with other proteins (5). Slit1 has been found mainly, but not exclusively, in the fetal and adult brain (2). Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary locations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hippocampal and motor axons (1, 6‑11). Deletion of either Slit1 or Slit2 has less effect than deletion of both, which allows axons to wander from tracts and inappropriately cross or recross the midline (6, 7, 9‑11). Expression of Slit1 by new neurons influences astrocytes to form and maintain tunnels that guide neuronal migration (12). In the injured spinal cord, presence of Slit1 along with Slit3 and Netrin-1 may be responsible for failure of axons to regenerate in the adult CNS (13). Slit1 also promotes dendrite growth and branching of cortical neurons indicating it may exert important influence on the final morphology of cortical neurons (14).

  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Hohenester, E. (2008) Biochem. Soc. Trans. 36:251.
  3. Hussain, S-A. et al. (2006) J. Biol. Chem. 281:39693.
  4. Accession # EAW49957.
  5. Accession # EAW49956 and CAH70917.
  6. Thompson, H. et al. (2006) Dev. Biol. 296:476.
  7. Plump, A. S. et al. (2002) Neuron 33:219.
  8. Cho, J. H. et al. (2007) J. Neurosci. 27:9094.
  9. Nguyen-Ba-Charvet, K. T. et al. (2002) J. Neurosci. 22:5473.
  10. Bagri, A. et al. (2002) Neuron 33:233.
  11. DiMeglio, T. et al. (2008) J. Neurosci. 28:6285.
  12. Kaneko, N. et al. (2010) Neuron 67:213.
  13. Wehrle, R. et al. (2005) Eur. J. Neurosci. 22:2134.
  14. Whitford, K. L. et al. (2002) Neuron 33:47.

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Publications for Slit1 (6514-SL)(2)

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Bioinformatics

Gene Symbol SLIT1
Entrez
Uniprot