ROBO2 Antibody

Images

 
Western Blot: ROBO2 Antibody [NBP1-81399] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: ROBO2 Antibody [NBP1-81399] - Staining of human cell line SH-SY5Y shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of mouse embryo E14 shows strong positivity in developing olfactory bulb and olfactory nerve.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in neuropil.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human kidney shows negative to very weak cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ROBO2 Antibody Summary

Immunogen
This antibody was developed against Recombinant ROBO2 Protein corresponding to amino acids: PVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWA
Specificity
Specificity of human ROBO2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROBO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockout Validated
Application Notes
IHC reported in scientific literature (PMID: 24597571). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockout validation (PMID: 32220420).
Control Peptide
ROBO2 Protein (NBP1-81399PEP)
Publications
Read Publications using
NBP1-81399 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • KO
    1 publication
  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24597571). Mouse, rat and monkey (PMID: 32220420).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ROBO2 Antibody

  • Drosophila) homolog 2
  • ROBO2
  • roundabout, axon guidance receptor, homolog 2 (Drosophila)
  • SAX3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, KO

Publications for ROBO2 Antibody (NBP1-81399)(2)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Monkey.

We have publications tested in 4 applications: ICC/IF, IHC, KO, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
KO
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(1)
Monkey
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROBO2 Antibody (NBP1-81399) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ROBO2 Antibody (NBP1-81399)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol ROBO2