Slit3 Antibody (782619)

Slit3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Slit3 Monoclonal Antibody (Catalog # MAB3629) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 782619
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Slit3 Antibody (782619) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Slit3
  Ser27-His901
  Accession # Q9WVB4
• Specificity: Detects mouse Slit3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Slit1, rmSlit2, or recombinant human Slit3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: SLIT3
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Slit3 Antibody (782619)

• KIAA0814
• MEGF5
• MEGF5Multiple epidermal growth factor-like domains protein 5
• Multiple EGF-like domains protein 5
• SLIL2
• SLIL2FLJ10764
• slit homolog 3 (Drosophila)
• slit homolog 3 protein
• SLIT1
• slit2
• Slit3
• Slit-3slit (Drosophila) homolog 3

Background

Slit3 is a member of the slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1). Like other mammalian family members, the 1523 amino acid (aa), 200 kDa Slit3 contains a signal sequence followed by 23 leucine-rich repeats (LRR) and nine EGF-like sequences (1). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7, and a C-terminal cysteine-rich domain (1). In Drosophila Slit, specific LRR are sites of ROBO interaction and homodimerization (2). Drosophila Slit shows equal similarities with all three mouse slit proteins, which are 59‑66% identical with each other (1). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in function (3). Slit3 is also expressed in the lung, kidney, skeletal muscle and heart, both during development and postnatally (1, 4‑6). ROBO2 is often found in complementary areas (4). Mice with genetically disrupted Slit3 have thin diaphragms with disorganized collagen fibrils and frequently develop diaphragmatic hernias (5, 6). Abnormalities in kidney development may also occur (5). Although Slit proteins are generally considered to be secreted, significant amounts of Slit3 may be retained in the mitochondria because of features in the signal sequence that indicate a high probability of mitochondrial targeting (7). Secreted Slit is often membrane-associated (7). Mouse Slit3 shows 98% and 94% amino acid identity with rat and human Slit3, respectively.

1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
2. Howitt, J. A. et al. (2004) EMBO J. 23:4406.
3. Long, H. et al. (2004) Neuron 42:213.
4. Greenberg, J. M. et al. (2004) Dev. Dyn. 230:350.
5. Liu, J. et al. (2003) Mech. Dev. 120:1059.
6. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
7. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLIT3
• Uniprot:
  • Q9WVB4()