ROBO3 Antibody

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ROBO3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO3
  Leu40-Ser545
  Accession # Q96MS0
• Specificity: Detects ROBO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse ROBO3 is observed, 5% cross-reactivity with recombinant human ROBO2 and recombinant rat ROBO1 is observed, and less than 1% cross‑reactivity with recombinant human ROBO4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO3 Antibody

• FLJ21044
• HGPPS
• HGPS
• RBIG1horizontal gaze palsy with progressive scoliosis
• retinoblastoma inhibiting gene 1
• RIG1
• ROBO3
• roundabout (axon guidance receptor, Drosophila) homolog 3
• roundabout homolog 3
• roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Roundabout-like protein 3

Background

Human ROBO3 is a 200 kDa member of the ROBO family of guidance molecules (1‑3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1386 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 474 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64‑531) and three fibronectin (FN) type III domains (aa 555‑863). The cytoplasmic region contains three of four possible 15‑20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO1 (7, 8). Human ROBO3 has multiple isoforms. An alternate start site generates a 1365 aa A isoform and a 1341 aa B isoform. These two mature forms differ only over the first 26 and 2 amino acids of the N-terminus, respectively. There are multiple point mutations and insertions in the ROBO3 gene. Three result in truncated forms. One is 456 aa in length and ends after the Ig-like domain # 4. A second is 770 aa in length and ends in the third FN domain. A third isoform is truncated after aa 1108 in the cytoplasmic region after CC2. At least one alternate splice form is also reported. It shows a 10 aa substitution between aa 1025‑1034, followed by truncation. Human ROBO3 ECD is 84% and 91% aa identical to the ECD in mouse and canine ROBO3, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. However, only ROBO3 Form B is known to bind to SLIT2 (9‑11).

1. Rajagopalan, S. et al. (2000) Neuron 28:767.
2. Guthrie, S. (2004) Curr. Biol. 14:R632.
3. Guthrie, S. (2001) Curr. Biol. 11:R300.
4. Seeger, M. et al. (1993) Neuron 10:409.
5. Jen, J.C. et al. (2004) Science 304:1509.
6. Swiss-Prot. Accession # Q96MS0.
7. Bashaw, G.J. et al. (2000) Cell 101:703.
8. Kidd, T. et al. (1998) Cell 92:205.
9. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
10. Sabatier, C. et al. (2004) Cell 117:157.
11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO3 Antibody (AF3076)(12)

Publications using AF3076

• Schlienger, S;Yam, PT;Balekoglu, N;Ducuing, H;Michaud, JF;Makihara, S;Kramer, DK;Chen, B;Fasano, A;Berardelli, A;Hamdan, FF;Rouleau, GA;Srour, M;Charron, F; Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control Science advances 2023-05-12 [PMID: 37172092] (IHC, Transgenic Mouse, Mouse)
• Y Zang, K Chaudhari, GJ Bashaw Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs Cell Reports, 2022-12-06;41(10):111785. 2022-12-06 [PMID: 36476876] (Immunoprecipitation, Western Blot, Human)
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020-11-24;33(8):108425. 2020-11-24 [PMID: 33238113] (IHC, Rat)
• Y Zhu, T Hirata, F Mackay, F Murakami Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation Sci Rep, 2020-07-16;10(1):11830. 2020-07-16 [PMID: 32678266] (IHC, Mouse)
• S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection Cell Rep, 2020-06-09;31(10):107741. 2020-06-09 [PMID: 32521266] (IHC, Mouse)
• WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies Cell Rep, 2019-12-10;29(11):3405-3420.e5. 2019-12-10 [PMID: 31825825] (IHC, Mouse)
• J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance Cell Rep, 2019-12-10;29(11):3356-3366.e3. 2019-12-10 [PMID: 31825820] (IHC, Mouse)
• M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord Cell Rep, 2019-03-19;26(12):3298-3312.e4. 2019-03-19 [PMID: 30893602] (Immunoprecipitation, Mouse)
• A Andreu-Cer, I Anselme, A Karam, C Laclef, M Catala, S Schneider- The ciliopathy gene ftm/rpgrip1l controls mouse forebrain patterning via region-specific modulation of hedgehog/gli signaling J. Neurosci., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692221] (IHC, Mouse)
• C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal Floor-plate-derived netrin-1 is dispensable for commissural axon guidance Nature, 2017-04-26;0(0):. 2017-04-26 [PMID: 28445456] (IHC, Mouse)
• Samelson B, Gore B, Whiting J, Nygren P, Purkey A, Colledge M, Langeberg L, Dell'Acqua M, Zweifel L, Scott J A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors. J Biol Chem, 2015-04-16;290(22):14107-19. 2015-04-16 [PMID: 25882844] (ICC, Human)
• Geutskens SB, Hordijk PL, van Hennik PB The chemorepellent Slit3 promotes monocyte migration. J. Immunol., 2010-11-15;185(12):7691-8. 2010-11-15 [PMID: 21078908] (Flow Cytometry, Human)

Review for ROBO3 Antibody (AF3076) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 05/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Embryonic spinal cord
• Species: Mouse
• Lot: WKJ0117041

Bioinformatics

• Gene Symbol: ROBO3
• Uniprot:
  • Q96MS0()