ROBO3 Antibody

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ROBO3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO3
  Leu40-Ser545
  Accession # Q96MS0
• Specificity: Detects ROBO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse ROBO3 is observed, 5% cross-reactivity with recombinant human ROBO2 and recombinant rat ROBO1 is observed, and less than 1% cross-reactivity with recombinant human ROBO4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO3 Antibody

• FLJ21044
• HGPPS
• HGPS
• RBIG1horizontal gaze palsy with progressive scoliosis
• retinoblastoma inhibiting gene 1
• RIG1
• ROBO3
• roundabout (axon guidance receptor, Drosophila) homolog 3
• roundabout homolog 3
• roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Roundabout-like protein 3

Background

Human ROBO3 is a 200 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO3 is a type I transmembrane glycoprotein that is synthesized as a 1386 amino acid (aa) precursor. It contains a 20 aa signal sequence, an 871 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 474 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 64-531) and three fibronectin (FN) type III domains (aa 555-863). The cytoplasmic region contains three of four possible 15-20 aa long CC (conserved cytoplasmic) motifs that are found in ROBO1 (7, 8). Human ROBO3 has multiple isoforms. An alternate start site generates a 1365 aa A isoform and a 1341 aa B isoform. These two mature forms differ only over the first 26 and 2 amino acids of the N-terminus, respectively. There are multiple point mutations and insertions in the ROBO3 gene. Three result in truncated forms. One is 456 aa in length and ends after the Ig-like domain # 4. A second is 770 aa in length and ends in the third FN domain. A third isoform is truncated after aa 1108 in the cytoplasmic region after CC2. At least one alternate splice form is also reported. It shows a 10 aa substitution between aa 1025-1034, followed by truncation. Human ROBO3 ECD is 84% and 91% aa identical to the ECD in mouse and canine ROBO3, respectively. Normally, axons originating on one side of the spinal cord are inhibited from crossing to the other side by a SLIT2-ROBO1 interaction at the midline. ROBO3 is permissive for this event. It is unclear how this is accomplished. One possibility is that it binds directly to ROBO1, blocking SLIT activation. A second possibility involves ROBO3 binding to SLIT2 in a nonproductive interaction. However, only ROBO3 Form B is known to bind to SLIT2 (9-11).

1. Rajagopalan, S. et al. (2000) Neuron 28:767.
2. Guthrie, S. (2004) Curr. Biol. 14:R632.
3. Guthrie, S. (2001) Curr. Biol. 11:R300.
4. Seeger, M. et al. (1993) Neuron 10:409.
5. Jen, J.C. et al. (2004) Science 304:1509.
6. Swiss-Prot. Accession # Q96MS0.
7. Bashaw, G.J. et al. (2000) Cell 101:703.
8. Kidd, T. et al. (1998) Cell 92:205.
9. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
10. Sabatier, C. et al. (2004) Cell 117:157.
11. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO3 Antibody (AF3076)(26)

Publications using AF3076

• Antonio Herrera, Anghara Menendez, Andrea Ochoa, Lídia Bardia, Julien Colombelli, Sebastian Pons Neurogenesis changes beta -catenin shipping address from adherens junctions to nucleus to booster axonal growth Development 2023-08-15 [PMID: 37519286] (Immunohistochemistry, Avian - Chicken)
• Antonio Herrera, Anghara Menendez, Andrea Ochoa, Lídia Bardia, Julien Colombelli, Sebastian Pons Neurogenesis changes beta -catenin shipping address from adherens junctions to nucleus to booster axonal growth Development 8/15/2023 [PMID: 37519286]
• Schlienger, S;Yam, PT;Balekoglu, N;Ducuing, H;Michaud, JF;Makihara, S;Kramer, DK;Chen, B;Fasano, A;Berardelli, A;Hamdan, FF;Rouleau, GA;Srour, M;Charron, F; Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control Science advances 2023-05-12 [PMID: 37172092] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Y Zang, K Chaudhari, GJ Bashaw Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs Cell Reports, 2022-12-06;41(10):111785. 2022-12-06 [PMID: 36476876] (Immunoprecipitation, Western Blot, Human)
• Maud Wurmser, Mridula Muppavarapu, Christine Mary Tait, Christophe Laumonnerie, Luz María González-Castrillón, Sara Ivy Wilson Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin Frontiers in Cell and Developmental Biology 2021-06-23 [PMID: 34249921]
• Maud Wurmser, Mridula Muppavarapu, Christine Mary Tait, Christophe Laumonnerie, Luz María González-Castrillón, Sara Ivy Wilson Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin Frontiers in Cell and Developmental Biology 6/23/2021 [PMID: 34249921]
• Verónica Company, Abraham Andreu-Cervera, M. Pilar Madrigal, Belén Andrés, Francisca Almagro-García, Alain Chédotal et al. Netrin 1-Mediated Role of the Substantia Nigra Pars Compacta and Ventral Tegmental Area in the Guidance of the Medial Habenular Axons Frontiers in Cell and Developmental Biology 6/8/2021 [PMID: 34169076]
• Karina Chaudhari, Madhavi Gorla, Chao Chang, Artur Kania, Greg J Bashaw Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion eLife 4/12/2021 [PMID: 33843588]
• Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells STAR Protocols 3/4/2021 [PMID: 33659900]
• Hugo Ducuing, Thibault Gardette, Aurora Pignata, Karine Kindbeiter, Muriel Bozon, Olivier Thoumine et al. SlitC-PlexinA1 mediates iterative inhibition for orderly passage of spinal commissural axons through the floor plate eLife 12/21/2020 [PMID: 33345773]
• RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020-11-24;33(8):108425. 2020-11-24 [PMID: 33238113] (IHC, Rat)
• Y Zhu, T Hirata, F Mackay, F Murakami Chemokine receptor CXCR7 non-cell-autonomously controls pontine neuronal migration and nucleus formation Sci Rep, 2020-07-16;10(1):11830. 2020-07-16 [PMID: 32678266] (IHC, Mouse)
• S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection Cell Rep, 2020-06-09;31(10):107741. 2020-06-09 [PMID: 32521266] (IHC, Mouse)
• WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies Cell Rep, 2019-12-10;29(11):3405-3420.e5. 2019-12-10 [PMID: 31825825] (IHC, Mouse)
• J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance Cell Rep, 2019-12-10;29(11):3356-3366.e3. 2019-12-10 [PMID: 31825820] (IHC, Mouse)
• M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord Cell Rep, 2019-03-19;26(12):3298-3312.e4. 2019-03-19 [PMID: 30893602] (Immunoprecipitation, Mouse)
• A Andreu-Cer, I Anselme, A Karam, C Laclef, M Catala, S Schneider- The ciliopathy gene ftm/rpgrip1l controls mouse forebrain patterning via region-specific modulation of hedgehog/gli signaling J. Neurosci., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692221] (IHC, Mouse)
• Chloé Dominici, Quentin Rappeneau, Pavol Zelina, Stéphane Fouquet, Alain Chédotal Non-cell autonomous control of precerebellar neuron migration by Slit and Robo proteins Development 2/8/2017 [PMID: 29343636]
• Kenta Yamauchi, Maya Yamazaki, Manabu Abe, Kenji Sakimura, Heiko Lickert, Takahiko Kawasaki et al. Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline Scientific Reports 9/20/2017 [PMID: 28931893]
• Kenta Yamauchi, Maya Yamazaki, Manabu Abe, Kenji Sakimura, Heiko Lickert, Takahiko Kawasaki et al. Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline Scientific Reports 2017-09-20 [PMID: 28931893] (Immunohistochemistry, Mouse)
• C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal Floor-plate-derived netrin-1 is dispensable for commissural axon guidance Nature, 2017-04-26;0(0):. 2017-04-26 [PMID: 28445456] (IHC, Mouse)
• SG Varadaraja, JH Kong, KD Phan, TJ Kao, SC Panaitof, J Cardin, H Eltzschig, A Kania, BG Novitch, SJ Butler Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord Neuron, 2017-04-21;0(0):. 4/21/2017 [PMID: 28434801]
• Berenice Hernandez-Enriquez, Zhuhao Wu, Edward Martinez, Olav Olsen, Zaven Kaprielian, Patricia F. Maness et al. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing Genes & Development 12/15/2015 [PMID: 26680304]
• Samelson B, Gore B, Whiting J, Nygren P, Purkey A, Colledge M, Langeberg L, Dell'Acqua M, Zweifel L, Scott J A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors. J Biol Chem, 2015-04-16;290(22):14107-19. 2015-04-16 [PMID: 25882844] (ICC, Human)
• Carmen Ruiz de Almodovar, Pierre J. Fabre, Ellen Knevels, Cathy Coulon, Inmaculada Segura, Patrick C.G. Haddick et al. VEGF Mediates Commissural Axon Chemoattraction through Its Receptor Flk1 Neuron 6/9/2011 [PMID: 21658588]
• Geutskens SB, Hordijk PL, van Hennik PB The chemorepellent Slit3 promotes monocyte migration. J. Immunol., 2010-11-15;185(12):7691-8. 2010-11-15 [PMID: 21078908] (Flow Cytometry, Human)

Review for ROBO3 Antibody (AF3076) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Ricardo Vieira IHC-Fr Mouse 05/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Embryonic spinal cord
• Species: Mouse
• Lot: WKJ0117041

Bioinformatics

• Gene Symbol: ROBO3
• Uniprot:
  • Q96MS0()