UNC5H2/UNC5B Antibody [Unconjugated]

Images

 
Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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UNC5H2/UNC5B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat UNC5H2
Gly27-Asp373
Accession # O08722
Specificity
Detects rat UNC5H2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) UNC5H3 and recombinant rat UNC5H1 is observed, and less than 1% cross-reactivity with rhUNC5H4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UNC5B
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC5H2/UNC5B Antibody [Unconjugated]

  • p53RDL1
  • p53-regulated receptor for death and life protein 1
  • Protein unc-5 homolog 2
  • Protein unc-5 homolog B
  • transmembrane receptor Unc5H2
  • unc5 (C.elegans homolog) b
  • unc-5 homolog 2
  • unc-5 homolog B (C. elegans)
  • UNC5B
  • UNC5H2
  • UNC5H2netrin receptor UNC5B

Background

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2).

UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

  1. Hong, K. et al. (1999) Cell 97:927.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO J. 20:2715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UNC5H2/UNC5B Antibody (AF1006)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Functional Assay, ICC, IHC Frozen, IHC Paraffin-embedded, In vivo, Neut, WB.


Filter By Application
Functional Assay
(1)
ICC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
In vivo
(2)
Neut
(2)
WB
(1)
All Applications
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Human
(2)
Mouse
(7)
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Showing Publications 1 - 9 of 9.
Publications using AF1006 Applications Species
K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 2017;119(0):26-39. 2017 [PMID: 28373074] (Functional Assay,, Mouse) Functional Assay Mouse
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014;20(4):377-84. 2014 [PMID: 24584118] (WB,, Mouse) WB Mouse
van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013;33(5):911-9. 2013 [PMID: 23430612] (Neut,, Human) Neut Human
Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013;32(5):629-44. 2013 [PMID: 23299940] (ICC,, Mouse) ICC Mouse
Konig K, Kohler D, Granja T, Jennewein C, Tran N, Mirakaj V, Krohnert F, Rosenberger P The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury. PLoS ONE, 2012;7(7):e41085. 2012 [PMID: 22848430] (In vivo,, Mouse) In vivo Mouse
Tadagavadi RK, Wang W Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury. J. Immunol., 2010;185(0):3750-8. 2010 [PMID: 20693423] (In vivo,, Mouse) In vivo Mouse
Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. Am. J. Pathol., 2009;175(3):1010-8. 2009 [PMID: 19700747] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Hoang S, Liauw J, Choi M Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 2009;29(2):385-97. 2009 [PMID: 18985053] (IHC Frozen,, Mouse) IHC Frozen Mouse
Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB Netrin-1 inhibits leukocyte migration in vitro and in vivo. Proc. Natl. Acad. Sci. U.S.A., 2005;102(41):14729-34. 2005 [PMID: 16203981] (Neut,, Human) Neut Human

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Bioinformatics

Gene Symbol UNC5B
Entrez
Uniprot