UNC5H2/UNC5B Antibody [Unconjugated]

Images

 
Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated ...read more
Expression of UNC5B-D, DCC, neogenin, and A2b in retinas of normal and OIR mice on P12, P17, and P21. (a) Expression of UNC5B-D, DCC, neogenin, and A2b in normal and OIR mice. (b) Relative grayscale of UNC5B-D, DCC, ...read more
RT-PCR analysis of netrin-1 receptor mRNA in retinas of OIR mice. The expressions of UNC5B, UNC5C, UNC5D, neogenin, A2b, and DCC are all detected in retinas of OIR mice, while UNC5A is not detected. Image collected and ...read more
Western blot analysis of the effects of UNC5B shRNA on UNC5B expression in murine retinas. Representative western blot (a) and relative expression of UNC5B in murine retinas (b). UNC5B expression is significantly ...read more
UNC5B expression outside the CNS.A) Relative UNC5B mRNA expression in brain, heart, intestine, blood, lung, kidney, liver and spleen in C57Bl/6 mice. B)–E) Flowcytometry of murine leukocytes gated and stained for the ...read more
UNC5B expression outside the CNS.A) Relative UNC5B mRNA expression in brain, heart, intestine, blood, lung, kidney, liver and spleen in C57Bl/6 mice. B)–E) Flowcytometry of murine leukocytes gated and stained for the ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

UNC5H2/UNC5B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat UNC5H2
Gly27-Asp373
Accession # O08722
Specificity
Detects rat UNC5H2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UNC5B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC5H2/UNC5B Antibody [Unconjugated]

  • p53RDL1
  • p53-regulated receptor for death and life protein 1
  • Protein unc-5 homolog 2
  • Protein unc-5 homolog B
  • transmembrane receptor Unc5H2
  • unc5 (C.elegans homolog) b
  • unc-5 homolog 2
  • unc-5 homolog B (C. elegans)
  • UNC5B
  • UNC5H2
  • UNC5H2netrin receptor UNC5B

Background

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2).

UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

  1. Hong, K. et al. (1999) Cell 97:927.
  2. Leonardo, E.D. et al. (1997) Nature 386:833.
  3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
  4. Corset, V. (2000) Nature 407:747.
  5. Merz, D.C. (2001) Genetics 158:1071.
  6. Llambi, F. et al. (2001) EMBO J. 20:2715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC5H2/UNC5B Antibody (AF1006)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Cell Migration, Functional Assay, ICC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Cell Migration
(1)
Functional Assay
(1)
ICC
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Neutralization
(6)
Western Blot
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF1006 Applications Species
W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis Scientific Reports, 2022-03-14;12(1):4352. 2022-03-14 [PMID: 35288626] (Cell Migration, Neutralization, Western Blot, Human, Mouse) Cell Migration, Neutralization, Western Blot Human, Mouse
K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann Endothelial Unc5B controls blood-brain barrier integrity Nature Communications, 2022-03-04;13(1):1169. 2022-03-04 [PMID: 35246514] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Sonia Taïb, Noël Lamandé, Sabrina Martin, Fanny Coulpier, Piotr Topilko, Isabelle Brunet Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve eLife 2022-01-12 [PMID: 35019839]
T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells Nat Commun, 2018-11-27;9(1):5022. 2018-11-27 [PMID: 30479344] (Neutralization, Mouse) Neutralization Mouse
Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2 Circulation 2018-11-20 [PMID: 29976569]
K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 2017-04-01;119(0):26-39. 2017-04-01 [PMID: 28373074] (Functional Assay, Mouse) Functional Assay Mouse
Dan Liu, Si-Qi Xiong, Lei Shang, Xiao-feng Tian, Jing Yang, Xiao-Bo Xia Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice BMC Ophthalmology 2014-12-01 [PMID: 25149138]
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014-03-02;20(4):377-84. 2014-03-02 [PMID: 24584118] (Western Blot, Mouse) Western Blot Mouse
van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9. 2013-02-21 [PMID: 23430612] (Neutralization, Human) Neutralization Human
Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013-01-08;32(5):629-44. 2013-01-08 [PMID: 23299940] (ICC, Mouse) ICC Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol UNC5B
Uniprot