ROBO1 Antibody

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ROBO1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
ROBO1 was detected in immersion fixed frozen sections of rat embryo (neural tube) using 5 µg/mL Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ROBO1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ROBO1
Lys19-Ile560
Accession # O55005
Specificity
Detects rat ROBO1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROBO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF1749 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO1 Antibody

  • DUTT1
  • DUTT1Drosophila) homolog 1
  • H-Robo-1
  • MGC131599
  • MGC133277
  • ROBO1
  • roundabout, axon guidance receptor, homolog 1 (Drosophila)

Background

Rat ROBO1 (also DUTT1) is a 170-200 kDa member of the four molecule ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Rat ROBO1 is a type I transmembrane (TM) glycoprotein that is synthesized as a 1651 amino acid (aa) precursor. It contains an 18 aa signal sequence, an 879 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 733 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 68-541) and three fibronectin (FN) type III domains (aa 561-864). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C3) (7, 8). Rat ROBO1 is likely to have at least one isoform. Based on the human and mouse gene, rat ROBO1 will utilize an alternate start site, creating an A (long) and B (short) isoform. The difference is the presence of a 32 aa extension at the N-terminus of the mature molecule (occurs in the A form) (9-12). Based on ROBO3 studies, this extension will impact the ability of ROBO1 to bind Slit (10). Rat ROBO1 ECD is 98% and 96% aa identical to the ECD in mouse and human ROBO1, respectively. ROBO1 serves as a repulsing molecule for axons that cross the midline. Initially, ROBO3 allows outgrowing axons to traverse the midline/floorplate. Once crossed, axons express ROBO1 which deflects neurites attempting to recross to the ipsilateral side (13). The chemorepulsant activity of ROBO1 is dependent on ROBO1 binding to SLIT1/2. Inhibition of ROBO1 is likely due to ROBO1-ROBO3 heterophilic binding (10, 13-15).

  1. Guthrie, S. (2001) Curr. Biol. 11:R300.
  2. Guthrie, S. (2004) Curr. Biol. 14:R632.
  3. Park, K.W. et al. (2003) Dev. Biol. 261:251.
  4. Seeger, M. et al. (1993) Neuron 10:409.
  5. Kidd, T. et al. (1998) Cell 92:205.
  6. Marillat, V. et al. (2002) J. Comp. Neurol. 442:130.
  7. Bashaw, G.J. et al. (2000) Cell 101:703.
  8. Jen, J.C. et al. (2004) Science 304:1509.
  9. Clark, K. et al. (2002) FEBS Lett. 523:12.
  10. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
  11. Sundaresan, V. et al. (1998) Mol. Cell. Neurosci. 11:29.
  12. GenBank Accession # O55005.
  13. Sabatier, C. et al. (2004) Cell 117:157.
  14. Liu, Z. et al. (2004) Mol. Cell. Neurosci. 26:232.
  15. Brose, K. et al. (1999) Cell 96:795.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROBO1 Antibody (AF1749)(7)

We have publications tested in 2 confirmed species: Mouse, Chicken.

We have publications tested in 4 applications: IHC, IHC Fresh, IHC Frozen, WB.


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IHC
(4)
IHC Fresh
(1)
IHC Frozen
(1)
WB
(1)
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Mouse
(6)
Chicken
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF1749 Applications Species
C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal Floor-plate-derived netrin-1 is dispensable for commissural axon guidance Nature, 2017;0(0):. 2017 [PMID: 28445456] (IHC,, Mouse) IHC Mouse
Mire E, Mezzera C, Leyva-Diaz E, Paternain AV, Squarzoni P, Bluy L, Castillo-Paterna M, Lopez MJ, Peregrin S, Tessier-Lavigne M, Garel S, Galceran J, Lerma J, Lopez-Bendito G Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth. Nat. Neurosci., 2012;15(8):1134-43. 2012 [PMID: 22772332] (IHC,, Mouse) IHC Mouse
Hernandez-Miranda LR, Cariboni A, Faux C, Ruhrberg C, Cho JH, Cloutier JF, Eickholt BJ, Parnavelas JG, Andrews WD Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain. J. Neurosci., 2011;31(16):6174-87. 2011 [PMID: 21508241] (WB,, Mouse) WB Mouse
Farmer WT, Altick AL, Nural HF, Dugan JP, Kidd T, Charron F, Mastick GS Pioneer longitudinal axons navigate using floor plate and Slit/Robo signals. Development, 2008;135(22):3643-53. 2008 [PMID: 18842816] (IHC Fresh,, Mouse) IHC Fresh Mouse
Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J. Neurosci., 2008;28(35):8698-708. 2008 [PMID: 18753371] (IHC Frozen,, Chicken) IHC Frozen Chicken
Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons. J. Neurosci., 2008;28(16):4244-9. 2008 [PMID: 18417704] (IHC,, Mouse) IHC Mouse
Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons. Dev. Biol., 2007;313(2):648-58. 2007 [PMID: 18054781] (IHC,, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol ROBO1
Uniprot