ACE/CD143 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 ...read more
ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ACE/CD143 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ACE/CD143
Leu35-Gln1264
Accession # P09470
Specificity
Detects mouse ACE in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human ACE is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACE
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Applications/Dilutions

Dilutions
  • Western Blot 0.05 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
AF1513 in the following applications:

Publications
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AF1513 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE/CD143 Antibody [Unconjugated]

  • ACE
  • ACE1angiotensin converting enzyme, somatic isoform
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
  • carboxycathepsin
  • CD143 antigen
  • CD143
  • DCP
  • DCP1
  • DCP1angiotensin-converting enzyme
  • dipeptidyl carboxypeptidase 1
  • Dipeptidyl carboxypeptidase I
  • EC 3.2.1.-
  • EC 3.4.15.1
  • Kininase II
  • MGC26566
  • MVCD3
  • peptidase P
  • testicular ECA

Background

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (1). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (2).

ACE is a type I membrane protein and exists in two isoforms (1). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar catalytic domains called N- and C-domains. Germinal ACE, found exclusively in the testes, comprises a single catalytic domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (3). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (1). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (4, 5).

  1. Corvol, P. et al. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.) p. 332, Academic Press, San Diego.
  2. Hu, J. et al. (2001) J. Biol. Chem. 276:47863.
  3. Kessler, S.P. et al. (2000) J. Biol. Chem. 275:26259.
  4. Eyries, M. et al. (2001) J. Biol. Chem. 276:5525.
  5. Alfalah, M. et al. (2001) J. Biol. Chem. 276:21105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ACE/CD143 Antibody (AF1513)(3)

Review for ACE/CD143 Antibody (AF1513) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Opsanus beta.

Reviews using AF1513:
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Western Blot ACE/CD143 AF1513
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reviewed by:
Kevin Schauer
WB Opsanus beta 02/15/2017
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Summary

ApplicationWestern Blot
Sample TestedAdult small intestine
SpeciesOpsanus beta
LotJWD011608A

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Recent Reviews

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Kevin Schauer
02/15/2017
Application: WB
Species: Opsanus beta

Bioinformatics

Gene Symbol ACE
Entrez
Uniprot