SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

Staining of human placenta shows weak membranous positivity in trophoblastic cells.

Staining of rat cerebellum shows strong positivity in noradrenergic fibers.

Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.

Staining of mouse brain shows strong positivity in noradrenergic fibers in the hippocampus.

Staining of human prostate shows strong positivity in sympathetic peripheral nerves.

Staining of mouse brain shows strong positivity in noradrenergic fibers in the cerebral cortex.

Staining of rat pons shows strong positivity in noradrenaline neurons in the locus coeruleus.

Staining of human locus coeruleus shows strong cytoplasmic positivity in noradrenaline neurons.

• Reactivity: Hu
• Applications: IHC
• Clone: CL3063
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SLC6A2/NET/Noradrenaline transporter Antibody (CL3063) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was developed using a recombinant protein derived from P23975, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC6A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SLC6A2/NET/Noradrenaline transporter Antibody (CL3063)

• NAT1
• NAT1neurotransmitter transporter
• NET
• NET1sodium-dependent noradrenaline transporter
• Noradrenalin Transporter
• Norepinephrine Transporter
• SLC6A2
• SLC6A5
• solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2
• Solute carrier family 6 member 2
• solute carrier family 6 member 5

Background

Norepinephrine Transporter [NET] (or noradrenaline transporter (NAT)) is a monoamine transporter that transports the neurotransmitter noradrenaline from the synapse back to its vesicles for storage until later use. It also appears to transport the neurotransmitter dopamine in the same way, but to a lesser degree. Studies have shown a decrease in NET levels in the locus coeruleus in patients diagnosed with major depression (Klimek et al., 1997). Cocaine, amphetamines and many therapeutic antidepressants, such as the SNRIs (Serotonin-norepinephrine reuptake inhibitors) and the tricyclic antidepressants (TCAs) act to raise noradrenaline. Furthermore, deficits in the NET gene have been associated with ADHD (Kim et al., 2006).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC6A2