RIPK3/RIP3 Antibody

Images

 
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIPK3/RIP3 antibody [NBP1-77299] at (A) 1 and (B) 2 ug/mL. Observed molecular weight ~60 kDa. Theoretical molecular weight 53 ...read more
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in Mouse Cell lines. 15 ug of lysates per lane.Antibodies: RIPK3/RIP3 Antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. ...read more
Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Immunohistochemistry analysis of a paraffin embedded mouse kidney tissue section using RIPK3/RIP3 Antibody [NBP1-77299] at 2.5 ug/mL concentration.
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis using RIPK3/RIP3 antibody [NBP1-77299]. L929 cell line (mouse). 20 ug whole cell lysate. Primary antibody: 1:2000. Secondary antibody: 1:20000. Western blot ...read more
Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 Antibody [NBP1-77299] - ICC/IF analysis of Rat's kidney cell culture using RIPK3/RIP3 Antibody [NBP1-77299] at 20 ug/mL concentration.
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in C2C12 Cells. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat ...read more
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Western Blot Validation in Human Cell Lines. 15 ug of lysates per lane. Antibodies: RIPK3/RIP3 antibody [NBP1-77299], (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. ...read more
Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Rat kidney tissue with RIPK3/RIP3 Antibody [NBP1-77299] at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, GS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RIPK3/RIP3 Antibody Summary

Immunogen
RIPK3/RIP3 Antibody was made to a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3. Amino Acid Squence: AQFGRGRGWQPFHK
Specificity
Mouse RIPK3/RIP3 Antibody has one isoform (486aa, 53 kDa). Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57 kDa), isoform 2 (252aa, 28 kDa) and isoform 3 (231aa, 25 kDa). Rat RIP3 also has one isoform (478aa, 52 kDa). NBP1-77299 can detect can detect isoforms of mouse and rat as well as human isoform 1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.5 ug/mL
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Immunoprecipitation 20 ug/mL
  • Gel Supershift Assay
  • Knockdown Validated
  • Knockout Validated
Application Notes
Gel supershift assay reported in scientific literature [PMID: 27721066]. Immunoprecipitation reported in scientific literature [PMID: 27861127]. RIPK3/RIP3 antibody validated for WB from a verified customer review. Knockout validation reported in scientific literature [PMID: 32246911]. Knockdown validation reported in scientific literature [PMID: 31655343]. ICC/IF, Immunohistochemistry, and Western Blot reported in multiple pieces of scientific literature.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
RIPK3/RIP3 Protein (NBP1-77299PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77299 in the following applications:

Publications
Read Publications using
NBP1-77299 in the following applications:

  • GS
    1 publication
  • 2 publications
  • IHC
    2 publications
  • IP
    4 publications
  • KD
    1 publication
  • KO
    1 publication
  • WB
    35 publications

Reactivity Notes

Human, mouse and rat reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RIPK3/RIP3 Antibody

  • EC 2.7.11.1
  • Receptor Interacting Serine/Threonine Kinase 3
  • Receptor-interacting protein 3
  • receptor-interacting serine/threonine-protein kinase 3
  • receptor-interacting serine-threonine kinase 3
  • RIP3
  • RIP-3
  • RIPK3
  • RIP-like protein kinase 3

Background

RIPK3, Receptor interacting serine/threonine kinase 3 or RIP-like protein kinase 3 (human RIPK3 isoform1 theoretical molecular weight 57kDa) is a cytosolic protein with an amino terminal active kinase domain. RIP kinases, RIPK1 and RIPK3 play a central role in the induction of necroptosis, a form of programmed and inflammatory cell death that is caspase-independent (1). Necroptosis is a form of programmed necrosis which is initiated through the activation of RIPK3 by various ligands such as Fas, LPS and TNF. Formation of the necrosome results from the interaction between RIPK1 and RIPK3 mediated through their RIP homotypic interaction motifs (RHIMs) (1, 2). Following necrosome formation, RIPK3 activation leads to the phosphorylation of mixed lineage kinase domain-like protein (MLKL) which induces its oligomerization and translocation to the cell membrane where it alters plasma membrane integrity (2). Loss of membrane integrity results in lytic cell death, release of damage associated molecular patterns (DAMPs) and inflammation (2, 3). Therefore, the interaction of RIPK3 with other RHIM proteins is necessary for the initiation of necroptosis, while the execution phase is dependent on the activation of MLKL (4).

RIPK3 has several phosphorylation sites that are required for its role in necroptosis. For example, serine204 in mouse, which is conserved in the human (serine199), is necessary for necroptosis while serine residues 232 and 227 are both required for RIPK3s interaction with MLKL (2). The core necroptosis proteins RIPK1/RIPK3 and MLKL are implicated in several disease states such as neurodegeneration, cardiovascular, hepatic and pulmonary disease (3).

References

1. Orozco, S., & Oberst, A. (2017). RIPK3 in cell death and inflammation: the good, the bad, and the ugly. Immunological Reviews. https://doi.org/10.1111/imr.12536

2. Dhuriya, Y. K., & Sharma, D. (2018). Necroptosis: A regulated inflammatory mode of cell death. Journal of Neuroinflammation. https://doi.org/10.1186/s12974-018-1235-0

3. Choi, M. E., Price, D. R., Ryter, S. W., & Choi, A. M. K. (2019). Necroptosis: A crucial pathogenic mediator of human disease. JCI Insight. https://doi.org/10.1172/jci.insight.128834

4. Lee, K.-H., & Kang, T.-B. (2019). The Molecular Links between Cell Death and Inflammasome. Cells. https://doi.org/10.3390/cells8091057

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77299
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, GS, KD, KO

Publications for RIPK3/RIP3 Antibody (NBP1-77299)(46)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: GS, ICC/IF, IHC, IP, KD, KO, WB.


Filter By Application
GS
(1)
ICC/IF
(2)
IHC
(2)
IP
(4)
KD
(1)
KO
(1)
WB
(35)
All Applications
Filter By Species
Human
(10)
Mouse
(30)
Rat
(6)
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Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NBP1-77299 Applications Species
Dai Q, Zhang Y, Liao X et al. Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway Frontiers in pharmacology Dec 16 2020 [PMID: 33390935] (WB, Mouse) WB Mouse
LingHu HR, Luo H, Gang L Bufalin Induces Glioma Cell Death by Apoptosis or Necroptosis Onco Targets Ther May 27 2020 [PMID: 32581545] (WB, IP, Human) WB, IP Human
Seo J, Seong D, Nam YW et al. Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex Cell Death Differ. May 26 2020 [PMID: 32457484] (WB, Human, Mouse) WB Human, Mouse
Lyu AR, Kim TH, Park SJ et al. Mitochondrial Damage and Necroptosis in Aging Cochlea Int J Mol Sci Apr 3 2020 [PMID: 32260310] (IHC, Mouse) IHC Mouse
Zhou H, Xu C, Lee H et al. Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing Mol Metab Mar 4 2020 [PMID: 32246911] (WB, KO, Mouse) WB, KO Mouse
Colijn S, Muthukumar V, Xie J et al. Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis Dis Model Mech Jan 17 2020 [PMID: 31953345] (WB, Mouse) WB Mouse
Popper B, Rammer MT, Gasparitsch M, et al. Neonatal obstructive nephropathy induces necroptosis and necroinflammation Sci Rep Dec 9 2019 [PMID: 31819111] (WB, Mouse) WB Mouse
Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019 Oct 23 [PMID: 31655343] (WB, KD, Mouse) WB, KD Mouse
Takezaki A, Tsukumo S I et al. A homozygous SFTPA1 mutation drives necroptosis of type II alveolar epithelial cells in patients with idiopathic pulmonary fibrosis. J Exp Med Feb 12 2019 [PMID: 31601679] (IP, Mouse) IP Mouse
RIPK3 Activation Leads to Cytokine Synthesis That Continues After Loss of Cell Membrane Integrity Orozco SL, Daniels BP, Yatim N et al. Cell Rep [PMID: 31461645] (WB, Mouse) WB Mouse
Show All 46 Publications.

Review for RIPK3/RIP3 Antibody (NBP1-77299) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: L929 cells.

Reviews using NBP1-77299:
Filter by Applications
WB
(1)
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Filter by Species
L929 cells
(1)
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Images Ratings Applications Species Date Details
Western Blot RIPK3/RIP3 NBP1-77299
Enlarge
5
reviewed by:
Anonymous
WB L929 cells 05/04/2020
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesL929 cells
Lot8337-1701

Comments

CommentsL929 cell line
Primary antibody: 1:2000,
Secondary antibody: 1:20000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RIPK3/RIP3 Antibody (NBP1-77299). (Showing 1 - 5 of 5 FAQs).

  1. What research areas can this product be used in?
    • All RIPK3/RIP3 products can be used in: Cancer, Cell Biology, Immunology, Necroptosis, Protein Kinase, Signal Transduction.
  2. What is the theoretical molecular weight for your RIPK3/RIP3 antibodies?
    • This depends on the immunogen. We have one RIPK3/RIP3 antibody [NBP1-77299] that is raised against murine RIP3. The theoretical molecular weight for this product is 53 kDa. The rest of our RIPK3/RIP3 antibodies are developed against human RIP3 and have a theoretical molecular weight of 56.7 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I am currently looking for a good antibody working as an ELISA capture antibody for mouse RIP3. Would you let me know if this application was tested during the development of your NovusBio (NBP1-77299) antibody.
    • Our product NBP1-77299 has been validated for use in ELISA. We tested this product in a direct ELISA format; it has not yet been tested in a sandwich ELISA format. The immunogen used to make this product falls between amino acids 473 to 486 of the mouse protein. If your detection antibody is outside of this range, it is possible that this combination will yield positive results.
  5. What human isoforms can be detected by this antibody?
    • RIPK3/RIP3 Antibody [NBP1-77299] can detect human isoform 1. This antibody can also detect isoforms of mouse and rat.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
05/04/2020
Application: WB
Species: L929 cells

Bioinformatics

Gene Symbol RIPK3
Entrez
Uniprot