RIPK3/RIP3 Antibody

Images

 
Western Blot: RIPK3/RIP3 Antibody [NBP1-77299] - Analysis of RIP3 in HeLa cell lysate with RIP3 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: RIPK3/RIP3 Antibody [NBP1-77299] - Immunofluorescence of RIP3 in Rat Kidney cells with RIP3 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: RIPK3/RIP3 Antibody [NBP1-77299] - Rat kidney tissue with RIPK3 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RIPK3/RIP3 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RIPK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This antibody can be used for detection of RIP3 by Western blot at 0.5 to 1 mg/ml. An approximately 57 kDa band can be detected.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
RIPK3/RIP3 Peptide (NBP1-77299PEP)
Publications
Read Publications using
NBP1-77299 in the following applications:

  • GS
    1 publication
  • IP
    2 publications
  • WB
    17 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26109138).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RIPK3/RIP3 Antibody

  • EC 2.7.11.1
  • Receptor-interacting protein 3
  • receptor-interacting serine/threonine-protein kinase 3
  • receptor-interacting serine-threonine kinase 3
  • RIP3
  • RIP-3
  • RIP3receptor interacting protein 3
  • RIPK3
  • RIP-like protein kinase 3

Background

Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kappaB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RIPK3/RIP3 Antibody (NBP1-77299)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: GS, IP, WB.


Filter By Application
GS
(1)
IP
(2)
WB
(17)
All Applications
Filter By Species
Human
(4)
Mouse
(16)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-77299 Applications Species
Martens S, Jeong M, Tonnus W et al. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury Cell Death Dis 2017 Jun 29 [PMID: 28661484] (WB, Mouse) WB Mouse
Sridharan H, Ragan KB, Guo H et al. Murine cytomegalovirus IE3-dependent transcription is required for DAI/ZBP1-mediated necroptosis EMBO Rep. 2017 Jun 12 [PMID: 28607035] (Mouse) Mouse
Gong YN, Guy C, Olauson H et al. ESCRT-III Acts Downstream of MLKL to Regulate Necroptotic Cell Death and Its Consequences. Cell. Apr 6 2017 [PMID: 28388412] (Mouse) Mouse
Ros U, Pena-Blanco A, Hanggi K et al. Necroptosis Execution Is Mediated by Plasma Membrane Nanopores Independent of Calcium. Cell Rep 2017 Apr 04 [PMID: 28380356] (WB, Mouse) WB Mouse
Yang SH, Lee DK, Shin J et al. Nec-1 alleviates cognitive impairment with reduction of Abeta and tau abnormalities in APP/PS1 mice. EMBO Mol Med. Nov 17 2016 [PMID: 27861127] (IP, WB, Mouse) IP, WB Mouse
Shindo R, Yamazaki S, Ohmuraya M et al. Short form FLICE-inhibitory protein promotes TNFalpha-induced necroptosis in fibroblasts derived from CFLARs transgenic mice. Biochem. Biophys. Res. Commun. Nov 4 2016 [PMID: 27721066] (GS, Mouse) GS Mouse
Qin D, Wang X, Li Y et al. MiR-223-5p and -3p Cooperatively Suppress Necroptosis in Ischemic/Reperfused Hearts. J. Biol. Chem. Aug 8 2016 [PMID: 27502281] (WB, Mouse) WB Mouse
Seo J, Lee EW, Sung H et al. CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3 Nat. Cell Biol. Mar 1 2016 [PMID: 26900751] (WB, IP, Human, Mouse) WB, IP Human, Mouse
Wertz IE, Newton K, Seshasayee D et al. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature. 2015 Dec 17 [PMID: 26649818] (WB, Mouse) WB Mouse
Yoon S, Bogdanov K, Kovalenko A, Wallach D et al. Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it Cell Death Differ. 2015 Jul 17 [PMID: 26184911] (WB, Mouse) WB Mouse
Show All 22 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RIPK3/RIP3 Antibody (NBP1-77299). (Showing 1 - 1 of 1 FAQs).

  1. I am currently looking for a good antibody working as an ELISA capture antibody for mouse RIP3. Would you let me know if this application was tested during the development of your NovusBio (NBP1-77299) antibody.
    • Our product NBP1-77299 has been validated for use in ELISA. We tested this product in a direct ELISA format; it has not yet been tested in a sandwich ELISA format. The immunogen used to make this product falls between amino acids 473 to 486 of the mouse protein. If your detection antibody is outside of this range, it is possible that this combination will yield positive results.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol RIPK3
Entrez
Uniprot