cIAP-1/HIAP-2 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, lystates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line were included. A specific band for cIAP‑1/HIAP‑2 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-HumancIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for cIAP‑1/HIAP‑2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) in the parental HeLa cell line, but is notdetectable in knockout HeLa cell line. Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) was used at 5 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

cIAP-1/HIAP-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human cIAP-1/HIAP-2
  His2-Ser618
  Accession # Q13490
• Specificity: Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BIRC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP-1/HIAP-2 Antibody [Unconjugated]

• API1Hiap-2
• apoptosis inhibitor 1
• baculoviral IAP repeat containing 2
• baculoviral IAP repeat-containing 2
• baculoviral IAP repeat-containing protein 2
• BIRC2
• cIAP1
• c-IAP1
• cIAP-1
• hIAP2
• HIAP-2
• IAP homolog B
• IAP2
• IAP-2
• Inhibitor of apoptosis protein 2
• MIHB
• MIHBHIAP2
• NFR2-TRAF signalling complex protein
• RING finger protein 48
• RNF48hiap-2
• TNFR2-TRAF-signaling complex protein 2

Background

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta .

1. Roy, N. et al. (1997) EMBO J. 23:6914.
2. Deveraux, Q. et al. (1997) Nature 388:300.
3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
4. Herrera, B. et al. (2002) FEBS Letters 520:93.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for cIAP-1/HIAP-2 Antibody (AF8181)(45)

Publications using AF8181

• EL Orth-He, HC Huang, SD Rao, Q Wang, Q Chen, CM O'Mara, AJ Chui, M Saoi, AR Griswold, A Bhattachar, DP Ball, JR Cross, DA Bachovchin Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation Cell Reports, 2023-01-16;0(0):111965. 2023-01-16 [PMID: 36649711] (Western Blot, Human)
• B Dumétier, A Zadoroznyj, J Berthelet, S Causse, J Allègre, P Bourgeois, F Cattin, C Racoeur, C Paul, C Garrido, L Dubrez cIAP1/TRAF2 interplay promotes tumor growth through the activation of STAT3 Oncogene, 2022-11-18;0(0):. 2022-11-18 [PMID: 36400972] (Western Blot, Human)
• Y Akizuki, M Morita, Y Mori, A Kaiho-Soma, S Dixit, A Endo, M Shimogawa, G Hayashi, M Naito, A Okamoto, K Tanaka, Y Saeki, F Ohtake cIAP1-based degraders induce degradation via branched ubiquitin architectures Nature Chemical Biology, 2022-10-31;0(0):. 2022-10-31 [PMID: 36316570] (Immunoprecipitation, Human)
• V Granqvist, C Holmgren, C Larsson Induction of interferon-&beta and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells PLoS ONE, 2021-03-26;16(3):e0248175. 2021-03-26 [PMID: 33770100] (Western Blot, Human)
• Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas Scientific Reports, 2021-03-18;11(1):6392. 2021-03-18 [PMID: 33737574] (Western Blot, Human)
• DE Muench, A Olsson, K Ferchen, G Pham, RA Serafin, S Chutipongt, P Dwivedi, B Song, S Hay, K Chetal, LR Trump-Durb, J Mookerjee-, K Zhang, JC Yu, C Lutzko, KC Myers, KL Nazor, KD Greis, DJ Kappes, SS Way, N Salomonis, HL Grimes Mouse models of neutropenia reveal progenitor-stage-specific defects Nature, 2020-04-22;582(7810):109-114. 2020-04-22 [PMID: 32494068] (IHC, Human)
• CC Nixon, M Mavigner, GC Sampey, AD Brooks, RA Spagnuolo, DM Irlbeck, C Mattingly, PT Ho, N Schoof, CG Cammon, GK Tharp, M Kanke, Z Wang, RA Cleary, AA Upadhyay, C De, SR Wills, SD Falcinelli, C Galardi, H Walum, NJ Schramm, J Deutsch, JD Lifson, CM Fennessey, BF Keele, S Jean, S Maguire, B Liao, EP Browne, RG Ferris, JH Brehm, D Favre, TH Vanderford, SE Bosinger, CD Jones, JP Routy, NM Archin, DM Margolis, A Wahl, RM Dunham, G Silvestri, A Chahroudi, JV Garcia Systemic HIV and SIV latency reversal via non-canonical NF-kappaB signalling in vivo Nature, 2020-01-22;578(7793):160-165. 2020-01-22 [PMID: 31969707] (IHC, Human)
• J Dittmann, T Haydn, P Metzger, GA Ward, M Boerries, M Vogler, S Fulda Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways Cell Death Differ., 2019-12-12;0(0):. 2019-12-12 [PMID: 31831875] (Western Blot, Human)
• LGT Lemos, GMDC Longo, BDS Mendonça, MC Robaina, MCM Brum, CA Cirilo, ERP Gimba, PRR Costa, CD Buarque, G Nestal de, RC Maia The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration Int J Mol Sci, 2019-10-12;20(20):. 2019-10-12 [PMID: 31614718] (Western Blot, Human)
• S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes Cell Rep, 2019-02-19;26(8):2194-2211.e6. 2019-02-19 [PMID: 30784599] (Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot, Human)
• J Allègre, J Cartier, V Glorian, N Droin, B Dumetier, C Kayaci, J Berthelet, S Gemble, C Vuillier, L Maillet, C Garrido, L Dubrez E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding PLoS ONE, 2018-10-25;13(10):e0206253. 2018-10-25 [PMID: 30359437] (Immunoprecipitation, Western Blot, Human)
• N Shibata, K Shimokawa, K Nagai, N Ohoka, T Hattori, N Miyamoto, O Ujikawa, T Sameshima, H Nara, N Cho, M Naito Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase Sci Rep, 2018-09-10;8(1):13549. 2018-09-10 [PMID: 30202081] (Western Blot, Human)
• GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695633] (Western Blot, Human)
• N Ohoka, Y Morita, K Nagai, K Shimokawa, O Ujikawa, I Fujimori, M Ito, Y Hayase, K Okuhira, N Shibata, T Hattori, T Sameshima, O Sano, R Koyama, Y Imaeda, H Nara, N Cho, M Naito Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation J. Biol. Chem., 2018-03-15;0(0):. 2018-03-15 [PMID: 29545311] (Western Blot, Human)
• A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment Cell Death Differ., 2017-11-03;0(0):. 2017-11-03 [PMID: 29099485] (Western Blot, Human)
• H Takaki, Y Akazawa, Y Kido, M Morishita, T Honda, H Shibata, S Miuma, H Miyaaki, N Taura, H Kondo, K Nakao Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis Med. Sci. Monit., 2017-09-21;23(0):4526-4532. 2017-09-21 [PMID: 28931802] (Western Blot, Human)
• V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017-08-10;0(0):. 2017-08-10 [PMID: 28799237] (Western Blot, Human)
• N Shibata, N Miyamoto, K Nagai, K Shimokawa, T Sameshima, N Ohoka, T Hattori, Y Imaeda, H Nara, N Cho, M Naito Development of protein degradation inducers of oncogenic BCR-ABL protein by conjugation of ABL kinase inhibitors and IAP ligands Cancer Sci., 2017-06-19;108(8):1657-1666. 2017-06-19 [PMID: 28556300] (Western Blot, Human)
• V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues Cell Death Dis, 2017-05-25;8(5):e2816. 2017-05-25 [PMID: 28542143] (Immunoprecipitation, Western Blot, Human)
• S Biswas, JL Shisler Molluscum Contagiosum Virus MC159 abrogates cIAP1-NEMO interactions and inhibits NEMO polyubiquitination J. Virol., 2017-07-12;0(0):. 2017-07-12 [PMID: 28515292] (Western Blot, Human)
• L Zhao, G Yang, H Bai, M Zhang, D Mou NCTD promotes Birinapant-mediated anticancer activity in breast cancer cells by downregulation of c-FLIP Oncotarget, 2017-04-18;8(16):26886-26895. 2017-04-18 [PMID: 28460471] (Western Blot, Human)
• L Hernandez, MK Kim, AM Noonan, E Sagher, H Kohlhammer, G Wright, LT Lyle, PS Steeg, M Anver, DD Bowtell, CM Annunziata A dual role for Caspase8 and NF-?B interactions in regulating apoptosis and necroptosis of ovarian cancer, with correlation to patient survival Cell Death Discov, 2015-12-14;1(0):15053. 2015-12-14 [PMID: 28179987] (Western Blot, Human)
• ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice Cell Death Dis, 2017-01-05;8(1):e2535. 2017-01-05 [PMID: 28055006] (IHC, Western Blot, Human, Mouse)
• Keiichiro Okuhira Targeted degradation of proteins localized in subcellular compartments by hybrid small molecules Mol. Pharmacol, 2016-12-13;0(0):. 2016-12-13 [PMID: 27965304] (Western Blot, Human)
• Lee E, Seong D, Seo J, Jeong M, Lee H, Song J USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Cell Death Differ, 2015-01-23;22(9):1463-76. 2015-01-23 [PMID: 25613375] (Western Blot, Human)
• Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs). Anticancer Res, 2014-12-01;34(12):7007-20. 2014-12-01 [PMID: 25503127] (Western Blot, Human)
• Schoeneberger H, Belz K, Schenk B, Fulda S Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death. Oncogene, 2014-11-10;34(31):4032-43. 2014-11-10 [PMID: 25381820] (Western Blot, Human)
• Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair. J Hematol Oncol, 2014-08-20;7(0):62. 2014-08-20 [PMID: 25139395] (Western Blot, Human)
• Smolewski P, Witkowska M, Zwolinska M, Cebula-Obrzut B, Majchrzak A, Jeske A, Darzynkiewicz Z, Ardelt W, Ardelt B, Robak T Cytotoxic activity of the amphibian ribonucleases onconase and r-amphinase on tumor cells from B cell lymphoproliferative disorders. Int J Oncol, 2014-04-28;45(1):419-25. 2014-04-28 [PMID: 24789756] (Flow Cytometry, Human)
• Guicciardi , Maria Eu, Werneburg , Nathan W, Bronk , Steven F, Franke , Adrian, Yagita , Hideo, Thomas , Gary, Gores , Gregory Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE, 2014-03-14;9(3):e92124. 2014-03-14 [PMID: 24633224] (Western Blot, Human)
• Uno M, Saitoh Y, Mochida K, Tsuruyama E, Kiyono T, Imoto I, Inazawa J, Yuasa Y, Kubota T, Yamaoka S NF-kappaB inducing kinase, a central signaling component of the non-canonical pathway of NF-kappaB, contributes to ovarian cancer progression. PLoS ONE, 2014-02-12;9(2):e88347. 2014-02-12 [PMID: 24533079] (Western Blot, Human)
• Wang Q, Chen W, Bai L, Chen W, Padilla M, Lin A, Shi S, Wang X, Lin Y Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway. J Biol Chem, 2014-01-14;289(9):5654-63. 2014-01-14 [PMID: 24425875] (Immunoprecipitation, Western Blot, Human)
• Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation. FASEB J, 2013-12-26;28(4):1938-46. 2013-12-26 [PMID: 24371121] (Western Blot, Human)
• Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells. Clin Cancer Res, 2013-02-12;19(7):1784-94. 2013-02-12 [PMID: 23403634] (Western Blot, Human)
• Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma. PLoS ONE, 2012-09-20;7(9):e45492. 2012-09-20 [PMID: 23029050] (Western Blot, Human)
• Varfolomeev , Eugene, Moradi , Elham, Dynek , Jasmin N, Zha , Jiping, Fedorova , Anna V, Deshayes , Kurt, Fairbrother , Wayne J, Newton , Kim, Le Couter , Jennifer, Vucic , Domagoj Characterization of ML-IAP protein stability and physiological role in vivo. Biochem J, 2012-11-01;447(3):427-36. 2012-11-01 [PMID: 22853455] (Western Blot, Human)
• Oberoi-Khanuja , Tripat K, Karreman , Christia, Larisch , Sarit, Rapp , Ulf R, Rajalingam , Krishnar Role of melanoma inhibitor of apoptosis (ML-IAP) protein, a member of the baculoviral IAP repeat (BIR) domain family, in the regulation of C-RAF kinase and cell migration. J Biol Chem, 2012-06-18;287(34):28445-55. 2012-06-18 [PMID: 22711539] (Western Blot, Human)
• Role of Apollon in human melanoma resistance to antitumor agents that activate the intrinsic or the extrinsic apoptosis pathways. Clin. Cancer Res., 2012-05-02;18(12):3316-27. 2012-05-02 [PMID: 22553342] (Flow Cytometry, Western Blot, Human)
• Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin K, Fulda S Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner. Oncogene, 2012-04-02;32(8):988-97. 2012-04-02 [PMID: 22469979] (Western Blot, Human)
• Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis. Leukemia, 2011-12-16;26(5):1020-9. 2011-12-16 [PMID: 22173242] (Western Blot, Human)
• Ikner A, Ashkenazi A TWEAK Induces Apoptosis through a Death-signaling Complex Comprising Receptor-interacting Protein 1 (RIP1), Fas-associated Death Domain (FADD), and Caspase-8. J. Biol. Chem., 2011-04-27;286(24):21546-54. 2011-04-27 [PMID: 21525013] (Western Blot, Human)
• Reddy RM, Yeow WS, Chua A, Nguyen DM, Baras A, Ziauddin MF, Shamimi-Noori SM, Maxhimer JB, Schrump DS Rapid and profound potentiation of Apo2L/TRAIL-mediated cytotoxicity and apoptosis in thoracic cancer cells by the histone deacetylase inhibitor Trichostatin A: the essential role of the mitochondria-mediated caspase activation cascade. Apoptosis, 2007-01-01;12(1):55-71. 2007-01-01 [PMID: 17136498] (Western Blot, Human)
• Balasubramanian S, Mani S, Shiraishi H, Johnston RK, Yamane K, Willey CD, Cooper G, Tuxworth WJ, Kuppuswamy D Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases. J. Mol. Cell. Cardiol., 2006-08-22;41(4):669-79. 2006-08-22 [PMID: 16928382] (Western Blot, Feline)
• Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC cIAP1 Localizes to the nuclear compartment and modulates the cell cycle. Cancer Res., 2005-01-01;65(1):210-8. 2005-01-01 [PMID: 15665297] (ICC, Western Blot, Human)
• Mitsiades CS, Mitsiades N, Poulaki V, Schlossman R, Akiyama M, Chauhan D, Hideshima T, Treon SP, Munshi NC, Richardson PG, Anderson KC Activation of NF-kappaB and upregulation of intracellular anti-apoptotic proteins via the IGF-1/Akt signaling in human multiple myeloma cells: therapeutic implications. Oncogene, 2002-08-22;21(37):5673-83. 2002-08-22 [PMID: 12173037] (Western Blot, Human)

Reviews for cIAP-1/HIAP-2 Antibody (AF8181) (4)

Average Rating: 4

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Human.

reviewed by: Anonymous - WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22553342
• Species: Human

reviewed by: Anonymous - WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22212761
• Species: Human

reviewed by: Anonymous - WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20074295
• Species: Human

reviewed by: Anonymous - WB Human 01/09/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22711539
• Species: Human

Bioinformatics

• Gene Symbol: BIRC2
• Uniprot:
  • Q13490()