cIAP-1/HIAP-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 (lane 1), human cIAP-2 (lane 2), or non-transfected (lane 3). PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of ...read more
cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-HumancIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 10 µg/mL ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 ...read more
Genetic Strategies: Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP‑1/HIAP‑2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific ...read more
cIAP‑1/HIAP‑2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human cIAP‑1/HIAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8181) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

cIAP-1/HIAP-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human cIAP-1/HIAP-2
His2-Ser618
Accession # Q13490
Specificity
Detects human cIAP-1/HIAP-2. Does not cross-react with recombinant human cIAP-2 or XIAP.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BIRC2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control
cIAP-1/HIAP-2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4
using
AF8181 in the following applications:

Publications
Read Publications using
AF8181 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cIAP-1/HIAP-2 Antibody [Unconjugated]

  • API1Hiap-2
  • apoptosis inhibitor 1
  • baculoviral IAP repeat containing 2
  • baculoviral IAP repeat-containing 2
  • baculoviral IAP repeat-containing protein 2
  • BIRC2
  • cIAP1
  • c-IAP1
  • cIAP-1
  • hIAP2
  • HIAP-2
  • IAP homolog B
  • IAP2
  • IAP-2
  • Inhibitor of apoptosis protein 2
  • MIHB
  • MIHBHIAP2
  • NFR2-TRAF signalling complex protein
  • RING finger protein 48
  • RNF48hiap-2
  • TNFR2-TRAF-signaling complex protein 2

Background

cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.

  1. Roy, N. et al. (1997) EMBO J. 23:6914.
  2. Deveraux, Q. et al. (1997) Nature 388:300.
  3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
  4. Herrera, B. et al. (2002) FEBS Letters 520:93.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF8181
Species: Hu
Applications: WB, Simple Western, IHC, KO

Publications for cIAP-1/HIAP-2 Antibody (AF8181)(38)

We have publications tested in 2 confirmed species: Human, Feline.

We have publications tested in 3 applications: Flow Cytometry, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
Immunoprecipitation
(3)
Western Blot
(33)
All Applications
Filter By Species
Human
(37)
Feline
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using AF8181 Applications Species
J Dittmann, T Haydn, P Metzger, GA Ward, M Boerries, M Vogler, S Fulda Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways Cell Death Differ., 2019;0(0):. 2019 [PMID: 31831875] (Western Blot, Human) Western Blot Human
LGT Lemos, GMDC Longo, BDS Mendonça, MC Robaina, MCM Brum, CA Cirilo, ERP Gimba, PRR Costa, CD Buarque, G Nestal de, RC Maia The LQB-223 Compound Modulates Antiapoptotic Proteins and Impairs Breast Cancer Cell Growth and Migration Int J Mol Sci, 2019;20(20):. 2019 [PMID: 31614718] (Western Blot, Human) Western Blot Human
S Lee, JY Lee, EW Lee, S Park, DH Kang, C Min, DJ Lee, D Kang, J Song, J Kwon, SW Kang Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-?-Induced Apoptosis via Different Routes Cell Rep, 2019;26(8):2194-2211.e6. 2019 [PMID: 30784599] (Immunoprecipitation, Human) Immunoprecipitation Human
J Allègre, J Cartier, V Glorian, N Droin, B Dumetier, C Kayaci, J Berthelet, S Gemble, C Vuillier, L Maillet, C Garrido, L Dubrez E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding PLoS ONE, 2018;13(10):e0206253. 2018 [PMID: 30359437] (Immunoprecipitation, Human) Immunoprecipitation Human
N Shibata, K Shimokawa, K Nagai, N Ohoka, T Hattori, N Miyamoto, O Ujikawa, T Sameshima, H Nara, N Cho, M Naito Pharmacological difference between degrader and inhibitor against oncogenic BCR-ABL kinase Sci Rep, 2018;8(1):13549. 2018 [PMID: 30202081] (Western Blot, Human) Western Blot Human
GA Ward, EJ Lewis, JS Ahn, CN Johnson, JF Lyons, V Martins, JM Munck, SJ Rich, T Smyth, NT Thompson, PA Williams, NE Wilsher, NG Wallis, G Chessari ASTX660, a novel non-peptidomimetic antagonist of cIAP1/2 and XIAP, potently induces TNF-? dependent apoptosis in cancer cell lines and inhibits tumor growth Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29695633] (Western Blot, Human) Western Blot Human
N Ohoka, Y Morita, K Nagai, K Shimokawa, O Ujikawa, I Fujimori, M Ito, Y Hayase, K Okuhira, N Shibata, T Hattori, T Sameshima, O Sano, R Koyama, Y Imaeda, H Nara, N Cho, M Naito Derivatization of inhibitor of apoptosis protein (IAP) ligands yields improved inducers of estrogen receptor ? degradation J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29545311] (Western Blot, Human) Western Blot Human
A Mohr, L Deedigan, S Jencz, Y Mehrabadi, L Houlden, SM Albarenque, RM Zwacka Caspase-10: a molecular switch from cell-autonomous apoptosis to communal cell death in response to chemotherapeutic drug treatment Cell Death Differ., 2017;0(0):. 2017 [PMID: 29099485] (Western Blot, Human) Western Blot Human
H Takaki, Y Akazawa, Y Kido, M Morishita, T Honda, H Shibata, S Miuma, H Miyaaki, N Taura, H Kondo, K Nakao Hepatitis C Virus Infection Increases c-Jun N-Terminal Kinase (JNK) Phosphorylation and Accentuates Hepatocyte Lipoapoptosis Med. Sci. Monit., 2017;23(0):4526-4532. 2017 [PMID: 28931802] (Western Blot, Human) Western Blot Human
V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma Cancer Med, 2017;0(0):. 2017 [PMID: 28799237] (Western Blot, Human) Western Blot Human
Show All 38 Publications.

Reviews for cIAP-1/HIAP-2 Antibody (AF8181) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using AF8181:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22553342
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22553342

Details

Detection NotesSee PMID 22553342

Comments

CommentsPublished: See PMID 22553342Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22212761
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22212761

Details

Detection NotesSee PMID 22212761

Comments

CommentsPublished: See PMID 22212761Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20074295
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20074295

Details

Detection NotesSee PMID 20074295

Comments

CommentsPublished: See PMID 20074295Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
WB Human 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22711539
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22711539

Details

Detection NotesSee PMID 22711539

Comments

CommentsPublished: See PMID 22711539Independent review from antibodyresource.com

Product General Protocols

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FAQs for cIAP-1/HIAP-2 Antibody (AF8181) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on cIAP-1/HIAP-2.

cIAP1 - An apoptotic regulator with implications in drug resistant cancers
Cellular inhibitor of apoptosis protein-1 (cIAP-1) is an anti-apoptotic protein that is able to bind to caspases and inhibit their activity. Additionally cIAP-1 contains a RING domain with E3 ubiquitin ligase activity that is able to mediate the r...  Read full blog post.

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Recent Reviews

4
5
0
4
4
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: WB
Species: Human

 
Anonymous
01/09/2015
Application: WB
Species: Human

 
Anonymous
01/09/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol BIRC2
Uniprot