Caspase-8 Antibody

Images

 
Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of ...read more
Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. WB image submitted by a verified customer ...read more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade ...read more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex ...read more
Upstream signaling mechanisms induced by secreted eIF5A in cardiac myocytes.(a) Secreted re-eIF5A (10 ug/ml) activated initiator caspases, including caspase-8 (IMG-5703; IMGENEX), caspase-10 (ab25045; Abcam), caspase-2 ...read more
Regressing tumors display enrichment of a type I interferon response, p53, and TNF/death receptor signaling networks. i) Example photograph of IHC staining for cleaved caspase 8 (above) and quantification of IHC ...read more
Analysis of the cell type susceptible to apoptotic cell death in the hypothalamus of IRS2-/- mice. (D) Colocalization of cleaved caspase-8 and GFAP, and of cleaved caspase-8 and NeuN, in the hypothalamus of WT, ND ...read more
TRAM is required for the pro-inflammatory polarization of macrophages by SLD-LPS. (A,B) Flow cytometry analysis of Ly6C (A) or CD200R (B) expression in WT or TRAM−/− live-cell-gated macrophages with geometric MFI ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caspase-8 Antibody Summary

Immunogen
Recombinant catalytically active human Caspase-8 protein.
Specificity
Detects Caspase-8 and cleavage products.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Simple Western reported by internal validation
  • Western Blot 1:1000-1:2000
Application Notes
In Simple Western internal validation: Cell lysates as sample type; separated by size; antibody dilution of 1:500; matrix was 12-230 kDa; detected by Chemiluminescence.
Theoretical MW
55.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Caspase-8 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-56116 in the following applications:

Publications
Read Publications using
NB100-56116 in the following applications:

Reactivity Notes

Possible reactivity with canine species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-8 Antibody

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

The caspase family of cysteine proteases has been shown to play a key role in apoptosis. Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases.Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Caspase-8 Antibody (NB100-56116)(72)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB, WB/IP.


Filter By Application
FLOW
(1)
ICC/IF
(8)
IF/IHC
(2)
IHC
(1)
IHC-Fr
(3)
IHC-P
(12)
WB
(22)
WB/IP
(1)
All Applications
Filter By Species
Human
(21)
Mouse
(18)
Rat
(10)
All Species
Showing Publications 1 - 10 of 72. Show All 72 Publications.
Publications using NB100-56116 Applications Species
Wang S, Chang CW, Huang J et al. Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models The Journal of clinical investigation 2023-10-26 [PMID: 37883181] (WB, Human) WB Human
Luk CT, Chan CK, Chiu F et al. Dual role of caspase 8 in adipocyte apoptosis and metabolic inflammation Diabetes 2023-09-12 [PMID: 37699387]
Subramanya S, Fernando R, Goswami M et al. Flow cytometric method for the detection and quantification of retinal cell death and oxidative stress Experimental eye research 2023-06-29 [PMID: 37393050] (FLOW, Mouse) FLOW Mouse
Zhang L, Zhu D, Jiang J et al. The ubiquitin E3 ligase MDM2 induces chemoresistance in colorectal cancer by degradation of ING3 Carcinogenesis 2023-06-06 [PMID: 37279970]
Zhou J, Xiang W, Zhang K et al. IL1RAP Knockdown in LPS-Stimulated Normal Human Astrocytes Suppresses LPS-Induced Reactive Astrogliosis and Promotes Neuronal Cell Proliferation Neurochemical research 2022-12-11 [PMID: 36502418]
Lee SH, Ok SH, Ahn SH et al. Lipid emulsion inhibits the cardiac toxicity caused by chloroquine via inhibition of reactive oxygen species production Korean journal of anesthesiology 2022-11-15 [PMID: 36377332] (WB, Rat) WB Rat
Crudele A, Smeriglio A, Ingegneri M et al. Hydroxytyrosol Recovers SARS-CoV-2-PLpro-Dependent Impairment of Interferon Related Genes in Polarized Human Airway, Intestinal and Liver Epithelial Cells Antioxidants 2022-07-27 [PMID: 36009185] (WB, Human) WB Human
Ok SH, Ahn SH, Lee SH et al. Lipid emulsion attenuates propranolol-induced early apoptosis in rat cardiomyoblasts Human & experimental toxicology 2022-06-23 [PMID: 35738838]
Montecillo-Aguado M, Tirado-Rodriguez B, Antonio-Andres G et al. Omega-6 Polyunsaturated Fatty Acids Enhance Tumor Aggressiveness in Experimental Lung Cancer Model: Important Role of Oxylipins International Journal of Molecular Sciences 2022-05-31 [PMID: 35682855] (IHC) IHC
WU S, HU S, FAN W et al. Nitrite exposure may induce infertility in mice J Toxicol Pathol 2022-02-28 [PMID: 35221497]
Show All 72 Publications.

Reviews for Caspase-8 Antibody (NB100-56116) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-56116:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Caspase-8 NB100-56116
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3
reviewed by:
Verified Customer
WB Mouse 08/16/2021
View

Summary

ApplicationWestern Blot
Sample TestedMouse Colon,Mouse whole cell lysate
SpeciesMouse
LotAR-17/CIQ-17
Western Blot Caspase-8 NB100-56116
Enlarge
5
reviewed by:
Verified Customer
WB Rat 05/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedNRK whole cell lysate
SpeciesRat

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-8 Antibody (NB100-56116). (Showing 1 - 3 of 3 FAQs).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
  2. Hello,I want to perform a Western using this antibody and be able to see both inactive and active forms of caspase-8 using mouse cell line. Can I see an example of such experiment at least with human cell line?
    • I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.
  3. We would like to know if any antigen retrieval is required for this antibody (NB100-56116)? And if antigen retrieval is required, could you let us know the recommended protocol?
    • The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for Caspase-8 Antibody (NB100-56116)

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Research Areas for Caspase-8 Antibody (NB100-56116)

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Blogs on Caspase-8.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Verified Customer
08/16/2021
Application: WB
Species: Mouse

Verified Customer
05/22/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol CASP8
Entrez
Uniprot