Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of ...read more
Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. WB image submitted by a verified customer ...read more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade ...read more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex ...read more
55.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The caspase family of cysteine proteases has been shown to play a key role in apoptosis. Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases.Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Caspase-8 Antibody (NB100-56116). (Showing 1 - 3 of 3 FAQs).
May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
Hello,I want to perform a Western using this antibody and be able to see both inactive and active forms of caspase-8 using mouse cell line. Can I see an example of such experiment at least with human cell line?
I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.
We would like to know if any antigen retrieval is required for this antibody (NB100-56116)? And if antigen retrieval is required, could you let us know the recommended protocol?
The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.
Caspase 3, the executioner of apoptosis Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru... Read full blog post.
Caspase 8 - a key mediator of apoptosis Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a... Read full blog post.
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