Caspase-8 Antibody - (active/cleaved)


Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of more
Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. WB image submitted by a verified customer more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade more
Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex more

Product Details

Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

Caspase-8 Antibody - (active/cleaved) Summary

Recombinant catalytically active human Caspase-8 protein.
This is specific for the active (cleaved) Caspase 8 protein, both large (17-21 kDa) and small (10-13 kDa) subunits.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Theoretical MW
55.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Caspase-8 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
NB100-56116 in the following applications:

Read Publications using
NB100-56116 in the following applications:

Reactivity Notes

Possible reactivity with canine species.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Whole antisera
0.05% Sodium Azide

Alternate Names for Caspase-8 Antibody - (active/cleaved)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • Dihydrotestosterone receptorHYSP1
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • spinal and bulbar muscular atrophy


The caspase family of cysteine proteases has been shown to play a key role in apoptosis. Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases.Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
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Publications for Caspase-8 Antibody (NB100-56116)(62)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB, WB/IP.

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Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using NB100-56116 Applications Species
Kearon Je, Kocherry Sc, Zoumboulakis D Et Al. GDNF requires HIF-1 alpha and RET activation for suppression of programmed cell death of enteric neurons by metabolic challenge Molecular and cellular neurosciences Jul 15 2021 [PMID: 34273501] (ICC/IF) ICC/IF
Li Y, Li Z, Quan Y et al. Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis iScience Jun 1 2021 [PMID: 34142044] (Mouse) Mouse
Fasolato S, Ruvoletto M, Nardo G et al. Low P66shc with High SerpinB3 Levels Favors Necroptosis and Better Survival in Hepatocellular Carcinoma Biology Apr 23 2021 [PMID: 33922660] (WB, Human) WB Human
Nishio A, Bolte FJ, Takeda K et al. Clearance of pegylated interferon by Kupffer cells limits NK cell activation and therapy response of patients with HBV infection Science translational medicine Mar 31 2021 [PMID: 33790025]
Arman T, Lynch KD, Goedken M, Clarke JD Sub-chronic microcystin-LR renal toxicity in rats fed a high fat/high cholesterol diet Chemosphere Oct 27 2020 [PMID: 33143886] (IHC-P, Rat) IHC-P Rat
Ruera CN, Miculan E, Perez F et al. Sterile inflammation drives multiple programmed cell death pathways in the gut J. Leukoc. Biol. Sep 18 2020 [PMID: 32946645] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Doganlar O, Doganlar ZB, Ovali MA et al. Melatonin regulates oxidative stress and apoptosis in fetal hearts of pinealectomised RUPP rats Hypertens Pregnancy Aug 14 2020 [PMID: 32791955]
Tummers B, Mari L, Guy CS et al. Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis Immunity May 10 2020 [PMID: 32428502]
Ando Y, Ohuchida K, Otsubo Y et al. Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5 PLoS ONE Jan 30 2020 [PMID: 31999765] (WB, Human) WB Human
Riegger J, Huber-Lang M, Brenner RE Crucial role of the terminal complement complex in chondrocyte death and hypertrophy after cartilage trauma Osteoarthr. Cartil. Jan 22 2020 [PMID: 31981738] (IHC-P, Human) IHC-P Human
Show All 62 Publications.

Reviews for Caspase-8 Antibody (NB100-56116) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB100-56116:
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Images Ratings Applications Species Date Details
Western Blot Caspase-8 NB100-56116
reviewed by:
Verified Customer
WB Mouse 08/16/2021


ApplicationWestern Blot
Sample TestedMouse Colon,Mouse whole cell lysate
Western Blot Caspase-8 NB100-56116
reviewed by:
Verified Customer
WB Rat 05/22/2015


ApplicationWestern Blot
Sample TestedNRK whole cell lysate


Blocking Details5% NFDM for 1 h

Primary Anitbody

Dilution RatioCaspase 8 Antibody [NB100-56116] 1:1000, overnight, 4 degree

Secondary Antibody

Secondary DescriptionPeroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG Secondary Antibody
Secondary Manufacturer Cat#Jackson ImmunoReasearch #95093
Secondary Concentration1:20000


Detection NotesECL using the Femto ECL ( Pierce #34095) and image on the Chemdoc. Exposure time: 20s

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-8 Antibody (NB100-56116). (Showing 1 - 3 of 3 FAQs).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
  2. Hello,I want to perform a Western using this antibody and be able to see both inactive and active forms of caspase-8 using mouse cell line. Can I see an example of such experiment at least with human cell line?
    • I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.
  3. We would like to know if any antigen retrieval is required for this antibody (NB100-56116)? And if antigen retrieval is required, could you let us know the recommended protocol?
    • The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Caspase-8.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Mouse

Verified Customer
Application: WB
Species: Rat


Gene Symbol CASP8