Caspase-8 Antibody - (active/cleaved)

Images

 
Western Blot: active/cleaved Caspase 8 Antibody [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. Image from verified customer review.
Immunohistochemistry: active/cleaved Caspase 8 Antibody [NB100-56116] - Formalin-fixed, paraffin-embedded sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain ...read more
Immunohistochemistry: active/cleaved Caspase 8 Antibody [NB100-56116] - Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caspase-8 Antibody - (active/cleaved) Summary

Immunogen
Recombinant catalytically active human Caspase-8 protein was used as immunogen.
Specificity
This is specific for the active (cleaved) Caspase 8 protein, both large (17-21 kDa) and small (10-13 kDa) subunits.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Caspase-8 Lysate (NBL1-08717)
Reviewed Applications
Read 1 Review rated 5
using
NB100-56116 in the following applications:

Publications
Read Publications using
NB100-56116 in the following applications:

Reactivity Notes

May react with dog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-8 Antibody - (active/cleaved)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-8 Antibody (NB100-56116)(34)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB, WB/IP.


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ICC/IF
(4)
IHC
(1)
IHC-Fr
(2)
IHC-P
(7)
WB
(10)
WB/IP
(1)
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Human
(10)
Mouse
(10)
Rat
(4)
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-56116 Applications Species
Li F, Wang T, Wang Z et al. Histone deacetylase inhibitor quisinostat activates caspase signaling and upregulates p53 acetylation to inhibit the proliferation of HepG2 cells Mol Med Rep 2017 Aug 24 [PMID: 28849080] (Human) Human
Liu PF, Hu YC, Kang BH et al. Expression levels of cleaved caspase-3 and caspase-3 in tumorigenesis and prognosis of oral tongue squamous cell carcinoma PLoS ONE 2017 Jul 12 [PMID: 28700659] (IHC-P, Human) IHC-P Human
Xiao J, Yao J, Jia L et al. Protective Effect of Met12, a Small Peptide Inhibitor of Fas, on the Retinal Pigment Epithelium and Photoreceptor After Sodium Iodate Injury. Invest. Ophthalmol. Vis. Sci. Mar 1 2017 [PMID: 28346613] (WB, Rat) WB Rat
Chi W, Hua X, Chen X et al. Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye. J. Autoimmun. 2017 Feb 23 [PMID: 28238526] (ICC/IF, Mouse) ICC/IF Mouse
Gao Y, Yang H, Fan Y et al. Hydrogen-Rich Saline Attenuates Cardiac and Hepatic Injury in Doxorubicin Rat Model by Inhibiting Inflammation and Apoptosis. Mediators of Inflammation. Dec 1 2016 [PMID: 28104928] (WB, Rat) WB Rat
Wu CC, Cheng CH, Lee YH et al. Ursolic Acid Triggers Apoptosis in Human Osteosarcoma Cells via Caspase Activation and the ERk1/2 MAPk Pathway. J. Agric. Food Chem. Jun 1 2016 [PMID: 27171502] (WB, Human) WB Human
Magarin M, Pohl T, Lill A et al. Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress. J. Mol. Cell. Cardiol. 2016 Apr 19 [PMID: 27106802] (IHC-P, Mouse) IHC-P Mouse
Fan W, Li X, Yao H et al. Neural differentiation and synaptogenesis in retinal development. Neural Regeneration Research. 2016 Mar 16 [PMID: 27073386] (IHC-P, Mouse) IHC-P Mouse
Cheng CH, Cheng YP, Chang IL et al. Dodecyl gallate induces apoptosis by upregulating the caspase-dependent apoptotic pathway and inhibiting the expression of anti-apoptotic Bcl-2 family proteins in human osteosarcoma cells. Mol Med Rep. 2015 Dec 24 [PMID: 26707422] (WB, Human)

Details:
Cleaved Caspase 8 antibody used for WB on dodecyl gallate (DG) induced MG-63 cells (human osteosarcoma cell line) at a dilution of 1:1000. Cells were treated with various concentration of DG (Figure 5A).
WB Human
Matzke-Ogi A, Jannasch K, Shatirishvili M et al. Inhibition of Tumor Growth and Metastasis in Pancreatic Cancer Models by interference with CD44v6 Signaling. Gastroenterology. 2015 Oct 24 [PMID: 26597578]
Show All 34 Publications.

Review for Caspase-8 Antibody (NB100-56116) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56116:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Caspase-8 NB100-56116
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reviewed by:
Anonymous
WB Rat 05/22/2015
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Summary

ApplicationWestern Blot
Sample TestedNRK whole cell lysate
SpeciesRat

Blocking

Blocking Details5% NFDM for 1 h

Primary Anitbody

Dilution RatioCaspase 8 Antibody [NB100-56116] 1:1000, overnight, 4 degree

Secondary Antibody

Secondary DescriptionPeroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG Secondary Antibody
Secondary Manufacturer Cat#Jackson ImmunoReasearch #95093
Secondary Concentration1:20000

Details

Detection NotesECL using the Femto ECL ( Pierce #34095) and image on the Chemdoc. Exposure time: 20s

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-8 Antibody (NB100-56116). (Showing 1 - 3 of 3 FAQs).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
  2. Hello,I want to perform a Western using this antibody and be able to see both inactive and active forms of caspase-8 using mouse cell line. Can I see an example of such experiment at least with human cell line?
    • I apologize but I do not have an example of a western blot using NB100-56116 to send you. However, we will guarantee it 100% in western blotting using human, mouse and rat samples.
  3. We would like to know if any antigen retrieval is required for this antibody (NB100-56116)? And if antigen retrieval is required, could you let us know the recommended protocol?
    • The requirement of antigen retrieval mainly depends upon for how long the samples were kept in the fixative solution. This step is generally optional if the fixation is done for a brief period, however, I would recommend you include retrieval step to be on the safer side. Different antigen retrieval methods can be found at our Support webpage. In our lab, we generally employ Heat Induced Epitope Retrieval (HIER) method and the protocol for that would be: 1.) Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0): 1.92 g Citric acid (anhydrous) + 1000 ml Distilled water; Mix to dissolve. Adjust pH to 6.0 with 1N NaOH and add 0.5 ml of Tween 20, mix well. 2.) Pre-heat steamer or water bath with staining dish containing Citrate Buffer until temperature reaches 95-100C. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. 3.) Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Caspase-8.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Recent Reviews

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Anonymous
05/22/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol CASP8
Entrez
Uniprot