Renin Antibody [Unconjugated] - 1

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Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Renin Antibody [Unconjugated] - 1 Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
Leu22-Arg402
Accession # P06281
Specificity
Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant rat Renin is observed, and less than 0.2% cross-reactivity with recombinant human Renin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ren1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture:Mouse Renin 1 Antibody (Catalog # AF4277)
Standard: Recombinant Mouse Renin (Catalog # 4277-AS)
Publications
Read Publications using
AF4277 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Renin Antibody [Unconjugated] - 1

  • angiotensin-forming enzyme
  • Angiotensinogenase
  • EC 3.4.23
  • EC 3.4.23.15
  • FLJ10761
  • HNFJ2
  • Prorenin
  • REN
  • renin precursor, renal
  • Renin

Background

Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin 1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro Renin shares 70% and 85% aa sequence identity with human and rat pro Renin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Renin Antibody (AF4277)(7)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


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IHC
(1)
IHC-P
(1)
Western Blot
(5)
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Mouse
(6)
Transgenic Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF4277 Applications Species
Y Funato, D Yamazaki, D Okuzaki, N Yamamoto, H Miki Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure Nature Communications, 2021;12(1):3683. 2021 [PMID: 34140503] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
BL Phipps, U Suwannasua, J Lucero, NA Mitchell, AK Lund Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice Toxicology reports, 2021;8(0):846-862. 2021 [PMID: 33948438] (IHC, Mouse) IHC Mouse
K Miyauchi, T Nakai, S Saito, T Yamamoto, K Sato, K Kato, M Nezu, M Miyazaki, S Ito, M Yamamoto, N Suzuki Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions EBioMedicine, 2021;64(0):103209. 2021 [PMID: 33508746] (Western Blot, Mouse) Western Blot Mouse
NM Novielli-K, M Jelen, K Barr, LJ Delailo, Q Feng, BE Isakson, R Gros, DW Laird Ablation of both Cx40 and Panx1 results in similar cardiovascular phenotypes exhibited in Cx40 knockout mice Biosci. Rep., 2019;0(0):. 2019 [PMID: 30745457] (IHC-P, Mouse) IHC-P Mouse
Komers R, Xu B, Fu Y, McClelland A, Kantharidis P, Mittal A, Cohen H, Cohen D Transcriptome-based analysis of kidney gene expression changes associated with diabetes in OVE26 mice, in the presence and absence of losartan treatment. PLoS ONE, 2014;9(5):e96987. 2014 [PMID: 24827579] (Western Blot, Mouse) Western Blot Mouse
Mayer S, Roeser M, Lachmann P Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression. J. Biol. Chem., 2012;287(29):24483-91. 2012 [PMID: 22645148] (Western Blot, Mouse) Western Blot Mouse
Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 2010;285(53):41935-46. 2010 [PMID: 20966072] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol Ren1
Uniprot