IFN-alpha/beta R1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFN‑ alpha / beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IFN‑ alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFN-alpha/beta R1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
  Lys28-Lys436
  Accession # AAA52730
• Specificity: Detects human IFN‑ alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IFN‑ alpha / beta RI is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNAR1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha/beta R1 Antibody [Unconjugated]

• alpha-type antiviral protein
• AVP
• beta-type antiviral protein
• CRF2-1
• Cytokine receptor class-II member 1
• Cytokine receptor family 2 member 1
• human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC
• IFN-alpha/beta R1
• IFN-alpha/beta receptor 1
• IFN-alpha-REC
• IFNAR
• IFNAR1
• IFN-aR1
• IFNBR
• IFNbR1
• IFN-bR1
• IFN-R-1
• interferon (alpha, beta and omega) receptor 1
• interferon alpha/beta receptor 1
• interferon-alpha/beta receptor alpha chain
• interferon-beta receptor 1
• Type I interferon receptor 1

Background

Type I interferons (IFN-alpha , IFN-beta , IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha/beta R1 Antibody (AF245)(9)

Publications using AF245

• Y Han, L Tan, T Zhou, L Yang, L Carrau, LA Lacko, M Saeed, J Zhu, Z Zhao, BE Nilsson-Pa, FT Lira Neto, C Cahir, AM Giani, JC Chai, Y Li, X Dong, D Moroziewic, NYSCF Glob, D Paull, T Zhang, S Koo, C Tan, R Danziger, Q Ba, L Feng, Z Chen, A Zhong, GJ Wise, JZ Xiang, H Wang, RE Schwartz, BR tenOever, SA Noggle, CM Rice, Q Qi, T Evans, S Chen A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants Cell Stem Cell, 2022-10-06;29(10):1475-1490.e6. 2022-10-06 [PMID: 36206731] (ICC, Human)
• Benjamin Krämer, Rainer Knoll, Lorenzo Bonaguro, Michael ToVinh, Jan Raabe, Rosario Astaburuaga-García et al. Early IFN-alpha signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19 Immunity 11/1/2021 [PMID: 34592166]
• Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity Frontiers in Immunology 1/14/2021 [PMID: 33584648]
• Trisomy 21 consistently activates the interferon response Elife, 2016-07-29;5(0):. 2016-07-29 [PMID: 27472900] (Western Blot, Human)
• Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis. PLoS Pathog, 2014-03-06;10(3):e1003976. 2014-03-06 [PMID: 24603698] (Flow Cytometry, Human)
• Suto A, Nakajima H, Tokumasa N, Takatori H, Kagami S, Suzuki K, Iwamoto I Murine plasmacytoid dendritic cells produce IFN-gamma upon IL-4 stimulation. J. Immunol., 2005-11-01;175(9):5681-9. 2005-11-01 [PMID: 16237058] (Neutralization, Mouse)
• Sakamoto E, Hato F, Kato T, Sakamoto C, Akahori M, Hino M, Kitagawa S Type I and type II interferons delay human neutrophil apoptosis via activation of STAT3 and up-regulation of cellular inhibitor of apoptosis 2. J. Leukoc. Biol., 2005-04-21;78(1):301-9. 2005-04-21 [PMID: 15845643] (Neutralization, Human)
• Lin RJ, Liao CL, Lin E, Lin YL Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection. J. Virol., 2004-09-01;78(17):9285-94. 2004-09-01 [PMID: 15308723] (Flow Cytometry, Human)
• Qu C, Moran TM, Randolph GJ Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC. J. Immunol., 2003-01-15;170(2):1010-8. 2003-01-15 [PMID: 12517968] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IFNAR1
• Uniprot:
  • AAA52730()