Atrial Natriuretic Peptide/ANP Antibody [Unconjugated]

Images

 
Western blot shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyoctyes (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Atrial Natriuretic ...read more
Atrial Natriuretic Peptide/ANP was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Atrial Natriuretic Peptide/ANP was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Goat Anti-Human Atrial Natriuretic Peptide/ANP Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for Atrial ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Atrial Natriuretic Peptide/ANP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Atrial Natriuretic Peptide/ANP
Asn26-Tyr151
Accession # P01160
Specificity
Detects human Atrial Natriuretic Peptide/ANP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BNP, rhANF, and rhPTCH-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPPA
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Atrial Natriuretic Peptide/ANP Antibody [Unconjugated]

  • ANF
  • ANP
  • ANPatriopeptin
  • ATFB6
  • atrial natriuretic factor
  • Atrial Natriuretic Peptide
  • Cardionatrin
  • CDD-ANF
  • natriuretic peptide A
  • natriuretic peptide precursor A
  • NPPA
  • PND
  • PNDcardionatrin
  • prepronatriodilatin

Background

ANP (Atril Natriuetic Peptide; also known as gamma -ANP, CDP/cardiodilatin-related peptide and CDD-ANF) is a secreted member of the natriuretic peptide family of molecules. It is expressed in multiple cell types, including atrial myocardiocytes, macrophages and select hypothalamic neurons. The C-terminus of ANP (or ANF) is reported to have a wide range of activities. It is best known to promote sodium and water excretion, but is also known to inhibit aldosterone and vasopressin secretion, while promoting testosterone and LH release. Human (pro)ANP is synthesized as a 153 amino acid (aa) preproprecursor that contains a 25 aa signal sequence, cardiodilatin-related peptide (aa 26-55), a prosequence (aa 56-123) and a C-terminal atrial natriuretic factor motif (aa 124-151) that may or may not be accompanied by a dibasic Arg fragment. Although multiple enzymes are reported to be active on proANP, transmembrane Corin would appear to be central to normal extracellular proteolytic processing and the generation of ANP. Notably, and depending upon the tissue, proANP can also be cleaved at alternate sites, including Ala120 and Arg126. There is at least one isoform variant that contains a 12 aa addition to the C-terminus of the proprecursor, resulting in an ANP that is 40 aa in length. Over aa 26-151 (proANP), human proANP shares 84% aa sequence identity with mouse proANP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NPPA
Entrez
Uniprot