NLRP3/NALP3 Antibody

Images

 
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from ...read more
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat ...read more
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Western blot image submitted by a verified customer review.
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody ...read more
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with ...read more
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis in mouse cell lysate.
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Regular aerobic exercise decreased myocardial inflammation in HFD rats. Representative Western blot analysis of NLRP3 and caspase-1. Image collected and cropped by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IM, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NLRP3/NALP3 Antibody Summary

Immunogen
This NLRP3/NALP3 antibody was raised against a portion of amino acids 1-50 of human NLRP3/NALP3.
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Immunomicroscopy
  • Immunoprecipitation
  • Knockdown Validated
Application Notes
Use in IP reported in scientific literature (PMID:).
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP2-12446 in the following applications:

Publications
Read Publications using
NBP2-12446 in the following applications:

  • 13 publications
  • IHC
    12 publications
  • 5 publications
  • IM
    1 publication
  • KD
    2 publications
  • WB
    57 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • anti-mouse NLRP3
  • anti-NLRP3
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3 mouse
  • NLRP3 polyclonal
  • NLRP3 rat
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1betaand IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (NBP2-12446)(97)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-P, IM, KD, WB.


Filter By Application
ICC/IF
(13)
IHC
(12)
IHC-P
(5)
IM
(1)
KD
(2)
WB
(57)
All Applications
Filter By Species
Human
(15)
Mouse
(25)
Rat
(39)
All Species
Showing Publications 1 - 10 of 97. Show All 97 Publications.
Publications using NBP2-12446 Applications Species
Mauro A, Bonaventura A, VecchiE A et al. The Role of NLRP3 Inflammasome in Pericarditis JACC: Basic to Translational Science Feb 6 2021 [PMID: 33665514] (IHC, Human) IHC Human
Nie C, Ding X, A R et al. Hydrogen gas inhalation alleviates myocardial ischemia-reperfusion injury by the inhibition of oxidative stress and NLRP3-mediated pyroptosis in rats Life sciences Feb 20 2021 [PMID: 33621592] (IHC-P, WB, Rat) IHC-P, WB Rat
Liang H, Matei N, Devin W et al. TGR5 activation attenuates neuroinflammation via Pellino3 inhibition of Caspase-8/NLRP3 after middle cerebral artery occlusion in rats Research Square Dec 1 2020 [PMID: 33531049] (WB, Rat) WB Rat
Yang G, Tang X, Tan L, et al. Upregulation of miR-144-3p protects myocardial function from ischemia-reperfusion injury through inhibition of TMEM16A Ca2+-activated chloride channel Human cell Jan 15 2021 [PMID: 33452670]
Lliberos C, Liew SH, Zareie P, et al. Evaluation of inflammation and follicle depletion during ovarian ageing in mice Scientific reports Jan 11 2021 [PMID: 33432051] (WB, Mouse) WB Mouse
Ma DC, Zhang NN, Zhang YN, Chen HS Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro Journal of ethnopharmacology Jan 7 2021 [PMID: 33421597]
Cheng Y, Cheng L, Gao X, et al. Covalent modification of Keap1 at Cys77 and Cys434 by pubescenoside a suppresses oxidative stress-induced NLRP3 inflammasome activation in myocardial ischemia-reperfusion injury Theranostics Jan 1 2021 [PMID: 33391509]
Wu D, Zhang H, Wu Q et al. Sestrin 2 protects against LPS-induced acute lung injury by inducing mitophagy in alveolar macrophages Life sciences Dec 23 2020 [PMID: 33359748] (WB, ICC/IF, IHC, Mouse) WB, ICC/IF, IHC Mouse
Li J, Tian S, Wang H et al. Protection of hUC-MSCs against neuronal complement C3a receptor-mediated NLRP3 activation in CUMS-induced mice Neurosci Lett Nov 5 2020 [PMID: 33161108]
Sukhanov S, Higashi Y, Yoshida T et al. The SGLT2 inhibitor Empagliflozin attenuates interleukin-17A-induced human aortic smooth muscle cell proliferation and migration by targeting TRAF3IP2/ROS/NLRP3/Caspase-1-dependent IL-1beta and IL-18 secretion J Virol Nov 4 2020 [PMID: 33160017]
Show All 97 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP2-12446) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP2-12446:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot NLRP3/NALP3 NBP2-12446
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3
reviewed by:
Anonymous
WB Rat 10/05/2020
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Summary

ApplicationWestern Blot
Sample TestedPlacental tissue
SpeciesRat
LotG

Comments

CommentsRat placenta lysates, reduced and denatured; 100 ug total protein resolved on 10% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Blocking with Rockland blocking reagent. Incubate membrane overnight at 4C with 1:500 dilution of NLRP3/NALP3 antibody. Washes with PBS-0.1% tween 20. Incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-rabbit IgG. Images taken with Odyssey V3.0.
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
4
reviewed by:
Anonymous
WB Mouse 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotPO number; E0496223
FileView PDF
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
4
reviewed by:
Chunhui Sun
WB Human 03/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBSA RT 1h

Details

Detection NotesECL
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
5
reviewed by:
Chunhui Sun
WB Human 01/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBSA, 5%, 1 hour, RT

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG
Secondary Concentration1:5000

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP2-12446). (Showing 1 - 5 of 5 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence corresponding to amino acids 1 - 50 of human NLRP3/NALP3.
  5. What epitopes are recognized by IMG-6668A (NBP2-12446) antibody?
    • Unfortunately we don't map the epitopes for any of our antibodies, and thus we don't know where this antibody binds to. However since it is a rabbit polyclonal antibody, we anticipate that the IgG molecules should cover the entire immunogen sequence, spanning from a.a. 1 - 50.

Secondary Antibodies

 

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Anonymous
10/05/2020
Application: WB
Species: Rat

Anonymous
08/06/2016
Application: WB
Species: Mouse

Chunhui Sun
03/26/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot