NLRP3/NALP3 Antibody

Images

 
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) human NALP3 transfected cell lysate this antibody, B) mouse NALP3 transfected cell lysate this antibody, and C) HEK293 ...read more
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of formalin fixed paraffin-embedded (FFPE) human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Image from verified customer review.
Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

NLRP3/NALP3 Antibody Summary

Immunogen
This antibody was raised against a portion of amino acids 1-50 of human NALP3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Application Notes
Use in IHC-P reported in scientific literature (PMID 25437532), IHC (PMID 25602171), ICC (PMID 27867451).
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP2-12446 in the following applications:

Publications
Read Publications using
NBP2-12446 in the following applications:

  • 1 publication
  • IHC
    4 publications
  • 2 publications
  • WB
    22 publications

Reactivity Notes

Reacts with New World Monkey. The amino acid sequence used as immunogen is 100% homologous in Human and Monkey and 86% homologous in Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for NLRP3/NALP3 Antibody

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • anti-mouse NLRP3
  • anti-NLRP3
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3 mouse
  • NLRP3 polyclonal
  • NLRP3 rat
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NALP3 (NACHT, LRR and PYD domains-containing protein 3, or NLRP3) belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. The NALP3 complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NALP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NALP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P
Supplier Logo

Publications for NLRP3/NALP3 Antibody (NBP2-12446)(37)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-P
(2)
WB
(22)
All Applications
Filter By Species
Human
(8)
Mouse
(8)
Rat
(12)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NBP2-12446 Applications Species
Yichen S, Ruoxi S, Zhouye J et al. Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats Life Sci. May 22 2018 [PMID: 29800538] (Rat) Rat
Yang Q, Fu C, Xiao J, Ye Z. Uric acid upregulates the adiponectin-adiponectin receptor 1 pathway in renal proximal tubule epithelial cells. Molecular Medicine Reports 2017 Dec 19 [PMID: 29359786] (Rat) Rat
Kosmidou C, Efstathiou NE, Hoang MV et al. Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration Sci Rep 2018 Jan 11 [PMID: 29323137] (WB, Mouse) WB Mouse
Szpigel A, Hainault I, Carlier A et al. Lipid environment induces ER stress, TXNIP expression and inflammation in immune cells of individuals with type 2 diabetes. Diabetologia. 2017 Oct 07 [PMID: 28988346] (Human) Human
Wang Y, Yu B, Wang L et al. Pioglitazone ameliorates glomerular NLRP3 inflammasome activation in apolipoprotein E knockout mice with diabetes mellitus PLoS ONE 2017 Jul 14 [PMID: 28708885] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Xu X, Chen Y, Song J et al. Mangiferin suppresses endoplasmic reticulum stress in perivascular adipose tissue and prevents insulin resistance in the endothelium. Eur J Nutr. Mar 27 2017 [PMID: 28349253] (WB) WB
Yin J, Zhao F, Chojnacki JE et al. NLRP3 Inflammasome Inhibitor Ameliorates Amyloid Pathology in a Mouse Model of Alzheimer's Disease. Mol. Neurobiol. Mar 2 2017 [PMID: 28255908] (Mouse) Mouse
Beckerman P, Bi-Karchin J, Park AS et al. Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice. Nat. Med. 2017 Feb 20 [PMID: 28218918]
Li M, Li H, Fang F et al. Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms. Neurosci Lett 2017 Feb 03 [PMID: 28011393] (Mouse) Mouse
Li Y, Zheng JY, Liu JQ et al. Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis. Front Immunol. Dec 22 2016 [PMID: 28003810] (IHC, Rat) IHC Rat
Show All 37 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP2-12446) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-12446:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
reviewed by:
Anonymous
WB Mouse 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotPO number; E0496223
FileView PDF
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
reviewed by:
Chunhui Sun
WB Human 03/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBSA RT 1h

Details

Detection NotesECL
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
reviewed by:
Chunhui Sun
WB Human 01/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBSA, 5%, 1 hour, RT

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG
Secondary Concentration1:5000

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP2-12446). (Showing 1 - 5 of 5 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence corresponding to amino acids 1 - 50 of human NLRP3/NALP3.
  5. What epitopes are recognized by IMG-6668A (NBP2-12446) antibody?
    • Unfortunately we don't map the epitopes for any of our antibodies, and thus we don't know where this antibody binds to. However since it is a rabbit polyclonal antibody, we anticipate that the IgG molecules should cover the entire immunogen sequence, spanning from a.a. 1 - 50.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NLRP3/NALP3 Antibody (NBP2-12446)

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
08/06/2016
Application: WB
Species: Mouse

Chunhui Sun
03/26/2015
Application: WB
Species: Human

Chunhui Sun
01/29/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot