NLRP3/NALP3 Antibody - BSA Free

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C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry ...read more
Lycium barbarum polysaccharides lessened the changes in expression of pyroptosis markers (NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N)) in Abeta1-40 ...read more
Hippocampal AMPK/Sirt1 and NFkappaB/NLRP3/IL-1beta signaling pathways in diabetic rats and the effects of aerobic exercise. Type 2 diabetes significantly decreases the activation of hippocampal AMPK (a) and the level of ...read more
Exercise training prevents HFD-induced upregulation of cardiac pyroptosis. Western blotting was performed to determine the components of inflammasome complex and pyroptosis in the left ventricle of mice hearts. (A) ...read more
NLRP3 deficiency in non-hematopoietic cells exacerbates GVHD and is not rescued by propionate. B6 WT and Gpr43-/- mice received BMT from either syngeneic B6 or allogeneic BALB/c donors. a) Representative immunoblots and ...read more
The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation ...read more
Cellular and embryonic localisation of CL-L1.A. Immunostaining of ATDC5 cells with the golgi marker 58K and CL-L1. CL-L1 shows localisation with golgi apparatus (white arrow). B. Laminin and CL-L1 coimmunolocalisation. ...read more
Role of NF-kappa B in glucose fluctuation-induced inflammasome-related myocardial fibrosis. (A) Immunofluorescence staining of NF-kappa B/p65 nucleation (n = 3 per group). (B,C) The phosphorylation level of NF-kappa ...read more
Effects of oltipraz on the expression of CD11b/c, PI3K, p-Akt, and p-IkB alpha in the hippocampus of the CCI-injured mice. The relative protein levels of (A) CD11b/c, (B) PI3K, (D) p-Akt, and (E) p-IKB alpha in the ...read more
LRRK2 regulation on alpha -synuclein levels. a Western blot analysis of basal alpha -synuclein and b p62 levels in neurons during mid and late differentiations. Blots show the alpha -synuclein soluble monomer of ...read more
ER stress is induced by acidosis activated OGR1-mediated signalling. Caco-2 cells were subjected to different pH medium, following 4–6 h incubation in pH 7.6 serum free medium. (A) Vector control Caco-2 (VC) and ...read more
Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified and gated as ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

NLRP3/NALP3 Antibody - BSA Free Summary

Immunogen
This NLRP3/NALP3 antibody was raised against a portion of amino acids 1-50 of human NLRP3/NALP3.
Predicted Species
Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 34993560)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:50
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 35792172)
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Immunomicroscopy
  • Immunoprecipitation reported in scientific literature (PMID 37029500)
  • Knockdown Validated
  • Western Blot 2 - 5 ug/mL
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP2-12446 in the following applications:

Publications
Read Publications using
NBP2-12446 in the following applications:

  • B/N
    1 publication
  • 1 publication
  • 2 publications
  • 35 publications
  • 18 publications
  • IHC
    7 publications
  • 5 publications
  • 17 publications
  • IM
    1 publication
  • IP
    1 publication
  • KD
    2 publications
  • KO
    1 publication
  • WB
    111 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34455059). Use in Rat reported in scientific literature (PMID:33814920).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody - BSA Free

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • anti-mouse NLRP3
  • anti-NLRP3
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3 mouse
  • NLRP3 polyclonal
  • NLRP3 rat
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP3/NALP3 Antibody (NBP2-12446)(222)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 13 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IM, IP, KD, KO, WB.


Filter By Application
B/N
(1)
ELISA
(1)
FLOW
(2)
ICC/IF
(35)
IF/IHC
(18)
IHC
(7)
IHC-Fr
(5)
IHC-P
(17)
IM
(1)
IP
(1)
KD
(2)
KO
(1)
WB
(111)
All Applications
Filter By Species
Human
(29)
Mouse
(61)
Rat
(65)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 222. Show All 222 Publications.
Publications using NBP2-12446 Applications Species
Sguizzato M, Ferrara F, Baraldo N et al. Bilosomes and Biloparticles for the Delivery of Lipophilic Drugs: A Preliminary Study Antioxidants 2023-11-21 [PMID: 38136145] (ICC/IF, Human) ICC/IF Human
Luo M, Peng Y, Lv D et al. LncRNA GAS5 downregulates NLRP3 inflammasome activation-mediated pyroptosis in sepsis-induced myocardial injury by targeting SIRT3/AMPK? Heliyon 2023-12-01 [PMID: 38076153] (IHC-P, Mouse)

Details:
Mouse hearts: Dilution 1:200
IHC-P Mouse
McElwain CJ, Musumeci A, Manna S et al. L-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitus Journal of reproductive immunology 2023-11-24 [PMID: 38029485] (WB, Human) WB Human
Chen T, Du H, Zhou H et al. Yinhuapinggan granule ameliorates lung injury caused by multidrug-resistant Acinetobacter baumannii via inhibiting NF-?B/NLRP3 pathway Heliyon 2023-11-01 [PMID: 38027639] (IHC-P, WB, Mouse)

Details:
IHC-P Dilution 1:50, WB Dilution 1:500
IHC-P, WB Mouse
Gao Q, Gao Z, Su M et al. Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation Stem cells international 2023-11-14 [PMID: 38020203]
Wei J, Leng L, Sui Y et al. Phenolic acids from Prunella vulgaris alleviate cardiac remodeling following myocardial infarction partially by suppressing NLRP3 activation Phytotherapy research : PTR 2023-11-22 [PMID: 37992723]
Song G, Wang J, Liu J, Ruan Y Dimethyl fumarate ameliorates erectile dysfunction in bilateral cavernous nerve injury rats by inhibiting oxidative stress and NLRP3 inflammasome-mediated pyroptosis of nerve via activation of Nrf2/HO-1 signaling pathway Redox Biology 2023-10-01 [PMID: 37931471] (IHC-P, Rat)

Details:
IHC-P dilution 1:50; WB Dilution 1:500
IHC-P Rat
Hitomi M, Venegas J, Kang SC, Eng C Differential cell cycle checkpoint evasion by PTEN germline mutations associated with dichotomous phenotypes of cancer versus autism spectrum disorder Oncogene 2023-11-01 [PMID: 37907589]
Liang Y, Zhou Y, Wang J, He Y Downregulation of fibromodulin attenuates inflammatory signaling and atrial fibrosis in spontaneously hypertensive rats with atrial fibrillation via inhibiting TLR4/NLRP3 signaling pathway Immunity, inflammation and disease 2023-10-01 [PMID: 37904680] (WB, Rat)

Details:
Dilutions 1:250
WB Rat
Yang L, Lu P, Qi X et al. Metformin inhibits inflammatory response and endoplasmic reticulum stress to improve hypothalamic aging in obese mice iScience 2023-09-01 [PMID: 37860765] (WB, Mouse) WB Mouse
Show All 222 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP2-12446) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NBP2-12446:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NLRP3/NALP3 NBP2-12446
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3
reviewed by:
Verified Customer
WB Rat 10/05/2020
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Summary

ApplicationWestern Blot
Sample TestedPlacental tissue
SpeciesRat
LotG

Comments

CommentsRat placenta lysates, reduced and denatured; 100 ug total protein resolved on 10% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Blocking with Rockland blocking reagent. Incubate membrane overnight at 4C with 1:500 dilution of NLRP3/NALP3 antibody. Washes with PBS-0.1% tween 20. Incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-rabbit IgG. Images taken with Odyssey V3.0.
Western Blot NLRP3/NALP3 NBP2-12446
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4
reviewed by:
Verified Customer
WB Mouse 08/06/2016
View

Summary

ApplicationWestern Blot
SpeciesMouse
LotPO number; E0496223
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
4
reviewed by:
Chunhui Sun
WB Human 03/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
Western Blot NLRP3/NALP3 NBP2-12446
Enlarge
5
reviewed by:
Chunhui Sun
WB Human 01/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP2-12446). (Showing 1 - 5 of 5 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence corresponding to amino acids 1 - 50 of human NLRP3/NALP3.
  5. What epitopes are recognized by IMG-6668A (NBP2-12446) antibody?
    • Unfortunately we don't map the epitopes for any of our antibodies, and thus we don't know where this antibody binds to. However since it is a rabbit polyclonal antibody, we anticipate that the IgG molecules should cover the entire immunogen sequence, spanning from a.a. 1 - 50.

Secondary Antibodies

 

Isotype Controls

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Research Areas for NLRP3/NALP3 Antibody (NBP2-12446)

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Blogs on NLRP3/NALP3.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

mFluor Violet Conjugated Antibodies

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Verified Customer
10/05/2020
Application: WB
Species: Rat

Verified Customer
08/06/2016
Application: WB
Species: Mouse

Chunhui Sun
03/26/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot