NLRP3/NALP3 Antibody - BSA Free

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - C-AR inhibited NLRP3 inflammasome activation in synovial tissue. NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining and Western blot and expressed as the mean +/- SD. *p < 0.05 vs. the model; #p < 0.05 vs. the indicated treatment. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2016.00532/full), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Western blot image submitted by a verified customer review.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/mL respectively. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/mL.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis in mouse cell lysate.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Regular aerobic exercise decreased myocardial inflammation in HFD rats. Representative Western blot analysis of NLRP3 and caspase-1. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2019.01286/full), licensed under a CC-BY licence.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NLRP3/NALP3 Antibody - BSA Free Summary

• Immunogen: This NLRP3/NALP3 antibody was raised against a portion of amino acids 1-50 of human NLRP3/NALP3.
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:50
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Immunomicroscopy
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 2 - 5 ug/mL
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID:34993560) Use in IP reported in scientific literature (PMID:). Use in IHC-Fr reported in scientific literature (PMID:35792172).
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34455059). Use in Rat reported in scientific literature (PMID:33814920).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody - BSA Free

• AGTAVPRL
• AII
• AII/AVP
• Angiotensin/vasopressin receptor AII/AVP-like
• anti-mouse NLRP3
• anti-NLRP3
• AVP
• C1orf7
• Caterpiller protein 1.1
• CIAS1
• CLR1.1
• Cold autoinflammatory syndrome 1 protein
• Cold autoinflammatory syndrome 1
• Cryopyrin
• Familial cold autoinflammatory syndrome
• FCAS
• FCU
• Leucine-rich repeat-, and PYD-containing protein 3
• Muckle-Wells syndrome
• MWS
• NACHT
• NACHT, LRR and PYD containing protein 3
• NACHT, LRR and PYD domains-containing protein 3
• NALP3
• NLR family, pyrin domain containing 3
• NLRP3 mouse
• NLRP3 polyclonal
• NLRP3 rat
• NLRP3
• Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
• PYPAF1
• PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP3/NALP3 Antibody (NBP2-12446)(166)

Publications using NBP2-12446

• Perez-Cremades D, Bueno-Beti C, Garcia-Gimenez JL et al. Extracellular histones trigger oxidative stress-dependent induction of the NF-kB/CAM pathway via TLR4 in endothelial cells Journal of physiology and biochemistry 2022-12-05 [PMID: 36464762] (WB, Human)
• Liu Y, Wang Y, Chen L et al. Effect of electroacupuncture on NF- kappa B and NLRP3 inflammasome in uterine tissues of rats with primary dysmenorrhea J. Acupunct. Tuina. Sci. 2019-06-18 (Rat)
• Wu M, Ding H, Tang X et al. Efficiency of a novel thermosensitive enema in situ hydrogel carrying Periplaneta americana extracts for the treatment of ulcerative colitis Frontiers in pharmacology 2023-02-08 [PMID: 36843930] (WB)
• Wang Y, Sadike D, Huang B et al. Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-?B pathway in LPC-induced demyelination Journal of neuroinflammation 2023-02-18 [PMID: 36803990] (IHC, Mouse)
• Lin W, Li Z, Liang G et al. TNEA therapy promotes the autophagic degradation of NLRP3 inflammasome in a transgenic mouse model of Alzheimer's disease via TFEB/TFE3 activation Journal of neuroinflammation 2023-02-02 [PMID: 36732771] (IHC-Fr, Mouse)
• Xiao Q, Kang Z, Liu C et al. Panax notoginseng Saponins Attenuate Cerebral Ischemia-Reperfusion Injury via Mitophagy-Induced Inhibition of NLRP3 Inflammasome in Rats Frontiers in Bioscience-Landmark 2022-11-03 [PMID: 36472098] (WB, Rat)
  
  Details:
  Dilution used 1:500
• Lin J, Lai X, Fan X et al. Oridonin Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting GSDMD-Mediated Pyroptosis Genes 2022-11-17 [PMID: 36421808] (WB, Mouse)
  
  Details:
  Dilutions: 1:1000
• Zhou J, Zhang C, Fang X et al. Activation of autophagy inhibits the activation of NLRP3 inflammasome and alleviates sevoflurane induced cognitive dysfunction in elderly rats Research Square 2022-08-10 (WB, Rat)
• Park E, Choi H, Truong CS, Jun HS The Inhibition of Autophagy and Pyroptosis by an Ethanol Extract of Nelumbo nucifera Leaf Contributes to the Amelioration of Dexamethasone-Induced Muscle Atrophy Nutrients 2023-02-04 [PMID: 36839161] (WB, Mouse)
• Chen MB, Li MH, Wu LY et al. Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation Biochemical pharmacology 2023-02-16 [PMID: 36806583] (IHC-Fr, WB, Mouse)
• Muela-Zarzuela IS, Suarez-Rivero JM, Gallardo-Orihuela A et al. NLRP1 inflammasome modulates senescence and senescence-associated secretory phenotype bioRxiv : the preprint server for biology 2023-02-10 [PMID: 36798300] (WB, Human)
• Zhang H, Xie Z, Peng Y et al. PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration Ecotoxicology and environmental safety 2023-03-01 [PMID: 36764072] (WB, IHC, Mouse)
• Yue RZ, Li YJ, Su BH et al. Atorvastatin reduces contrast media-induced pyroptosis of renal tubular epithelial cells by inhibiting the TLR4/MyD88/NF-?B signaling pathway BMC nephrology 2023-02-02 [PMID: 36732683] (WB)
• Liu J, Liu B, He X et al. Dendorbium Nobile Lindl. Alkaloids Suppress NF-kappa B and NLRP3 Signaling Pathways to Attenuate Lipopolysaccharide-induced Neuroinflammation scientificarchives.com 2020-01-01 (ICC/IF, Mouse)
• Li S, Li H, Zhangdi H et al. Hair follicle-MSC-derived small extracellular vesicles as a novel remedy for acute pancreatitis Journal of controlled release : official journal of the Controlled Release Society 2022-11-19 [PMID: 36402231] (IHC-Fr, Mouse)
• Zheng L, Xu H, Zheng F et al. Intervention time decides the status of autophagy, NLRP3 activity and apoptosis in macrophages induced by ox-LDL Lipids in health and disease 2022-10-25 [PMID: 36284323] (ICC/IF, Human)
• Yamashita S, Takasu C, Morine Y et al. Characteristic submucosal alteration in biliary carcinogenesis of pancreaticobiliary maljunction with a focus on inflammasome activation Journal of hepato-biliary-pancreatic sciences 2022-10-19 [PMID: 36259178]
• Li M, Wu L, Chen M et al. Co-activation of Caspase-1 and Caspase-8 in CMV-induced SGN death by inflammasome-associated pyroptosis and apoptosis International immunopharmacology 2022-10-14 [PMID: 36244217] (IF/IHC, Mouse)
• Zhou Z, Ying C, Zhou X et al. Aerobic exercise training alleviates renal injury in db/db mice through inhibiting Nox4-mediated NLRP3 inflammasome activation Experimental gerontology 2022-08-22 [PMID: 36007721] (WB, Mouse)
• Eggelbusch M, Shi A, Broeksma BC et al. The NLRP3 inflammasome contributes to inflammation-induced morphological and metabolic alterations in skeletal muscle Journal of cachexia, sarcopenia and muscle 2022-08-17 [PMID: 35978267] (ICC/IF, WB, Human)
• Wu J, Chen S, Wu P et al. Cathepsin B/HSP70 complex induced by Ilexsaponin I suppresses NLRP3 inflammasome activation in myocardial ischemia/reperfusion injury Phytomedicine 2022-07-01 [PMID: 35952578] (WB, Rat)
• Yoon B, Yun Y, Kim KB, Kim DE Inhibition of immunoproteasome attenuates NLRP3 inflammasome formation in tumor necrosis factor alpha-stimulated intestinal epithelial cell Biochemical and biophysical research communications 2022-10-08 [PMID: 35944388]
• Tabandeh MR, Jozaie S, Ghotbedin Z, Gorani S Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfk beta, NLRP3, JNK signaling pathways Research in veterinary science 2022-08-01 [PMID: 35933803] (WB, Bovine)
• Beltran-Garcia J, Osca-Verdegal R, Perez-Cremades D et al. Extracellular Histones Activate Endothelial NLRP3 Inflammasome and are Associated with a Severe Sepsis Phenotype Journal of inflammation research 2022-07-25 [PMID: 35915852] (WB, Human)
• Luo M, Hu Y, Lv D et al. Recurrent Hypoglycemia Impaired Vascular Function in Advanced T2DM Rats by Inducing Pyroptosis Oxidative medicine and cellular longevity 2022-07-23 [PMID: 35915611] (IF/IHC, WB, Rat)
  
  Details:
  WB Dilution: 1:1000, IHC Dilution: 1:200
• Zhou L, Zhang J, Han X et al. CysLT2R Antagonist HAMI 3379 Ameliorates Post-Stroke Depression through NLRP3 Inflammasome/Pyroptosis Pathway in Gerbils Brain sciences 2022-07-24 [PMID: 35892417]
• Ge C, Zhao Y, Liang Y, He Y Silencing of TLR4 Inhibits Atrial Fibrosis and Susceptibility to Atrial Fibrillation via Downregulation of NLRP3-TGF-Beta in Spontaneously Hypertensive Rats Disease markers 2022-07-11 [PMID: 35860690] (WB, Rat)
  
  Details:
  Sprague-Dawley (SD) neonatal rats (1–3 days old), total protein was extracted from left atrial tissue and atrium fibroblasts, antibody used at 1:250
• Xie D, Hu G, Chen C et al. Loss of sphingosine kinase 2 protects against cisplatin induced-kidney injury American journal of physiology. Renal physiology 2022-07-14 [PMID: 35834271]
• Mavrogiannis E, Hagdorn Q, Bazioti V et al. Pirfenidone ameliorates pulmonary arterial pressure and neointimal remodeling in experimental pulmonary arterial hypertension by suppressing NLRP3 inflammasome activation Pulmonary Circulation 2022-06-17 [PMID: 35833096] (IF/IHC, Rat)
• Zhou H, Zhang W, Qin D et al. Activation of NLRP3 inflammasome contributes to the inflammatory response to allergic rhinitis via macrophage pyroptosis International immunopharmacology 2022-07-06 [PMID: 35809382] (IHC-P, WB, Human, Mouse)
• Liu B, Wang D, Cao Y et al. MitoTEMPO protects against podocyte injury by inhibiting NLRP3 inflammasome via PINK1/Parkin pathway-mediated mitophagy European journal of pharmacology 2022-08-15 [PMID: 35792172] (IHC-Fr, Rat)
• Liu X, Fan T, Guan J et al. Dopamine relieves inflammatory responses through the D2 receptor after electroacupuncture at ST36 in a mouse model of chronic obstructive pulmonary disease Acupuncture in medicine : journal of the British Medical Acupuncture Society 2022-07-01 [PMID: 35775581]
• Saieva S, Taglialatela G Near-infrared light reduces glia activation and modulates neuroinflammation in the brains of diet-induced obese mice Scientific reports 2022-06-27 [PMID: 35761012] (ICC/IF, Mouse)
• Al-Qazazi R, Lima PDA, Prisco SZ Et al. Macrophage-NLRP3 Activation Promotes Right Ventricle Failure in Pulmonary Arterial Hypertension Am J Respir Crit Care Med 2022-06-14 [PMID: 35699679] (WB, ICC/IF, FLOW, Human, Rat)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Lian H, Fang X, Li Q et al. NLRP3 Inflammasome-Mediated Pyroptosis Pathway Contributes to the Pathogenesis of Candida albicans Keratitis Frontiers in medicine 2022-04-06 [PMID: 35463001] (ICC/IF, Mouse)
• Wu Z, Zhou G, Wang H, Yao P Inhibition of KIF23 Alleviates IPAH by Targeting Pyroptosis and Proliferation of PASMCs International journal of molecular sciences [PMID: 35457254]
• Fang J, Ouyang M, Qu Y et al. Advanced glycation end products promote melanogenesis via activating NLRP3 in ammasome in human dermal fibroblasts The Journal of investigative dermatology 2022-04-11 [PMID: 35421403]
• Zhang C, Huang Y, Ouyang F et al. Extracellular vesicles derived from mesenchymal stem cells alleviate neuroinflammation and mechanical allodynia in interstitial cystitis rats by inhibiting NLRP3 inflammasome activation Journal of neuroinflammation 2022-04-06 [PMID: 35387668] (WB, Rat)
• Zou R, Nie C, Pan S et al. Co-administration of hydrogen and metformin exerts cardioprotective effects by inhibiting pyroptosis and fibrosis in diabetic cardiomyopathy Free radical biology & medicine 2022-03-15 [PMID: 35304269] (WB, IHC-P, Mouse)
• Shiau AL, Fang SY, Hsu CH et al. Prothymosin alpha Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury Acta Cardiologica Sinica 2022-03-01 [PMID: 35273440] (WB, Rat)
• Liu H, Wang LL, Xu QH et al. UHRF1 shapes both the trophoblast invasion and decidual macrophage differentiation in early pregnancy FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-04-01 [PMID: 35262949]
• Li, Y;Shi, D;Zhang, H;Yao, X;Ren, K; Magnetic Resonance Imaging of Contrast-Induced Acute Renal Injury and Related Pathological Alterations In Vivo Analytical cellular pathology (Amsterdam) [PMID: 35256925] (IF/IHC, Mouse)
• ye y, Feng Z, Tian S et al. HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation Oxidative medicine and cellular longevity 2022-02-07 [PMID: 35178162] (WB, Mouse)
• Oh Y, Kang H, Seo E, Jun H TGF-Beta activates NLRP3 Inflammasome by an Autocrine Production of TGF- Beta in LX-2 Human Hepatic Stellate Cells Mol Cell Biochem 2022-02-09 [PMID: 35138513]
• Li C, Zhu Y, Wu Y et al. Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome via Activation of Autophagy Frontiers in medicine 2022-01-12 [PMID: 35096901] (IHC-P, Mouse)
• Chen X, Lin S, Dai S et al. Trimetazidine affects pyroptosis by targeting GSDMD in myocardial ischemia/reperfusion injury Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2022-02-01 [PMID: 34993560] (WB, FLOW)
• Zhu Y, Huang G, Yang Y et al. Chinese Herbal Medicine Suyin Detoxification Granule Inhibits Pyroptosis and Epithelial-Mesenchymal Transition by Downregulating MAVS/NLRP3 to Alleviate Renal Injury Journal of inflammation research 2021-12-07 [PMID: 34908861]
• Park M, Kim HM, Shin HA Et al. Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model International journal of molecular sciences 2021-11-20 [PMID: 34830410] (WB, Rat)
• Hosoya T, Uchida S, Shibata S Et al. Xanthine Oxidoreductase Inhibitors Suppress the Onset of Exercise-induced AKI in High HPRT Activity Urat1-Uox Double Knockout Mice Journal of the American Society of Nephrology : JASN 2021-11-19 [PMID: 34799437] (WB, Mouse)
• Liu Y, Shang L, Zhou J Et al. Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo Inflammation 2021-11-17 [PMID: 34787801] (WB, Rat)
• Lauro F, Giancotti L, Kolar G et al. Role of Adenosine Kinase In Sphingosine-1-Phosphate Receptor 1-Induced Mechano-Hypersensitivities Research Square 2021-09-21 [PMID: 34773542] (WB, Rat)
• Penolazzi L, Bergamin LS, Lambertini E Et al. The P2X7 purinergic receptor in intervertebral disc degeneration Journal of cellular physiology 2021-10-19 [PMID: 34668208] (ICC/IF, IHC-P, Human)
• Wu J, Raman A, Coffey NJ Et al. The key role of NLRP3 and STING in APOL1-associated podocytopathy The Journal of clinical investigation 2021-10-15 [PMID: 34651582] (IF/IHC, Mouse)
• Wei B, Gong Y, Yang H Et al. Role of tumor necrosis factor receptor associated factor 6 in pyroptosis during acute pancreatitis Molecular medicine reports 2021-12-01 [PMID: 34643253] (WB, Human)
• Luo D, Dai W, Feng X Et al. Suppression of lncRNA NLRP3 inhibits NLRP3-triggered inflammatory responses in early acute lung injury Cell death & disease 2021-10-01 [PMID: 34599154] (WB, Rat)
• Huang J, An Q, Ju BM et al. Role of vitamin D/VDR nuclear translocation in down-regulation of NF- kappa B/NLRP3/caspase-1 axis in lupus nephritis International immunopharmacology 2021-09-15 [PMID: 34536747] (WB, Mouse)
• Tang B, She X, Deng C Effect of the combination of astragaloside IV and Panax notoginseng saponins on pyroptosis and necroptosis in rat models of cerebral ischemia reperfusion Experimental and Therapeutic Medicine 2021-08-04 [PMID: 34504577] (WB, Rat)
• Guo S, Geng X, Lee H, Ding Y Phenothiazine Inhibits Neuroinflammation and Inflammasome Activation Independent of Hypothermia After Ischemic Stroke Research Square 2021-07-07 [PMID: 34455546]
• Feng X, Zhan F, Luo D Et al. LncRNA 4344 promotes NLRP3-related neuroinflammation and cognitive impairment by targeting miR-138-5p Brain, behavior, and immunity 2021-08-26 [PMID: 34455059] (WB, Rat)
• Nie C, Zou R, Pan S Et al. Hydrogen gas inhalation ameliorates cardiac remodelling and fibrosis by regulating NLRP3 inflammasome in myocardial infarction rats Journal of cellular and molecular medicine 2021-08-16 [PMID: 34402164] (IHC-P, WB, Rat)
• ZHOU X, Yin K, Jiang W et al. Jiangzhi Ligan Decoction Alleviated Nonalcoholic Fatty Liver Disease Induced by High-Fat-Diet in Rats via GSDMD-mediated Canonical/Non-canonical Pyroptosis Pathway Dis Markers 2021-07-09 [PMID: 34239622]
• Dai S, Ye B, Chen L et al. Emodin alleviates LPS induced myocardial injury through inhibition of NLRP3 inflammasome activation Phytotherapy Research 2021-06-16 [PMID: 34131970]
• Lin X, Zhan J, Jiang J, Ren Y Upregulation of Neuronal Cylindromatosis Expression is Essential for Electroacupuncture-Mediated Alleviation of Neuroinflammatory Injury by Regulating Microglial Polarization in Rats Subjected to Focal Cerebral Ischemia/Reperfusion Journal of inflammation research 2021-05-20 [PMID: 34045881] (IF/IHC, Rat)
• Song X, Cui Z, He J et al. k-opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in APP/PS1 mice Molecular medicine reports 2021-07-01 [PMID: 34036389] (IHC-P, Mouse)
• Feng X, Hu J, Zhan F et al. MicroRNA-138-5p Regulates Hippocampal Neuroinflammation and Cognitive Impairment by NLRP3/Caspase-1 Signaling Pathway in Rats Journal of Inflammation Research 2021-03-01 [PMID: 33814920] (WB, Rat)
• Wang W, Cao Z, Liang H et al. Effect of low-dose ethanol on NLRP3 inflammasome in diabetes-induced lung injury Experimental animals 2021-04-02 [PMID: 33814530]
• Mauro A, Bonaventura A, VecchiE A et al. The Role of NLRP3 Inflammasome in Pericarditis JACC: Basic to Translational Science 2021-02-06 [PMID: 33665514] (IF/IHC, Human)
• Wu M, Ma Y, Chen X et al. Hyperuricemia causes kidney damage by promoting autophagy and NLRP3-mediated inflammation in rats with urate oxidase deficiency Disease models & mechanisms 2021-03-24 [PMID: 33648977] (WB, Rat)
• Nie C, Ding X, A R et al. Hydrogen gas inhalation alleviates myocardial ischemia-reperfusion injury by the inhibition of oxidative stress and NLRP3-mediated pyroptosis in rats Life sciences 2021-02-20 [PMID: 33621592] (WB, IHC-P, Rat)
• Liang H, Matei N, Devin W et al. TGR5 activation attenuates neuroinflammation via Pellino3 inhibition of Caspase-8/NLRP3 after middle cerebral artery occlusion in rats Research Square 2020-12-01 [PMID: 33531049] (WB, Rat)
• Yang G, Tang X, Tan L, et al. Upregulation of miR-144-3p protects myocardial function from ischemia-reperfusion injury through inhibition of TMEM16A Ca2+-activated chloride channel Human cell 2021-01-15 [PMID: 33452670]
• Lliberos C, Liew SH, Zareie P, et al. Evaluation of inflammation and follicle depletion during ovarian ageing in mice Scientific reports 2021-01-11 [PMID: 33432051] (WB, Mouse)
• Wang, M, Liu, Z Et al. Taohong Siwu Decoction Ameliorates Ischemic Stroke Injury Via Suppressing Pyroptosis. Front Pharmacol 2021-01-12 [PMID: 33424599] (ICC/IF, Human)
• Ma DC, Zhang NN, Zhang YN, Chen HS Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro Journal of ethnopharmacology 2021-01-07 [PMID: 33421597]
• Cheng Y, Cheng L, Gao X, et al. Covalent modification of Keap1 at Cys77 and Cys434 by pubescenoside a suppresses oxidative stress-induced NLRP3 inflammasome activation in myocardial ischemia-reperfusion injury Theranostics 2021-01-01 [PMID: 33391509]
• Wu D, Zhang H, Wu Q et al. Sestrin 2 protects against LPS-induced acute lung injury by inducing mitophagy in alveolar macrophages Life sciences 2020-12-23 [PMID: 33359748] (WB, IF/IHC, ICC/IF, Mouse)
• Li J, Tian S, Wang H et al. Protection of hUC-MSCs against neuronal complement C3a receptor-mediated NLRP3 activation in CUMS-induced mice Neurosci Lett 2020-11-05 [PMID: 33161108]
• Sukhanov S, Higashi Y, Yoshida T et al. The SGLT2 inhibitor Empagliflozin attenuates interleukin-17A-induced human aortic smooth muscle cell proliferation and migration by targeting TRAF3IP2/ROS/NLRP3/Caspase-1-dependent IL-1beta and IL-18 secretion J Virol 2020-11-04 [PMID: 33160017]
• Ghoneim M, Abdallah D, Shebl A, El-Abhar H The interrupted cross-talk of inflammatory and oxidative stress trajectories signifies the effect of artesunate against hepatic ischemia/reperfusion-induced inflammasomopathy Toxicology and Applied Pharmacology 2020-10-01 [PMID: 33130049]
• Lu Q, Zhang Y, Kasetti RB et al. Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway Invest Ophthalmol Vis Sci 2020-10-08 [PMID: 33085740] (WB, Rat)
• Bustos F, Segarra-Fas A, Nardocci G et al. Functional Diversification of SRSF Protein Kinase to Control Ubiquitin-Dependent Neurodevelopmental Signaling Dev Cell 2020-10-10 [PMID: 33080171] (ICC/IF, Rat)
• Gao P, Zha Y, Gong X et al. The role of maternal foetal interface inflammation mediated by NLRP3 inflammasome in the pathogenesis of recurrent spontaneous abortion Placenta 2020-10-06 [PMID: 33022545]
• Ma DC, Zhang NN, Zhang YN, Chen HS Kv1.3 channel blockade alleviates cerebral ischemia/reperfusion injury by reshaping M1/M2 phenotypes and compromising the activation of NLRP3 inflammasome in microglia Exp. Neurol. 2020-07-08 [PMID: 32652099] (WB, ICC/IF, Rat)
• Yang M, So KF, Lo ACY, Lam WC The Effect of Lycium barbarum Polysaccharides on Pyroptosis-Associated Amyloid beta1-40 Oligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage Int J Mol Sci 2020-06-30 [PMID: 32629957] (ICC/IF, Human)
• Zhang X, Fan X, Li F et al. Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats Mol Med Report 2020-06-16 [PMID: 32626997] (ICC/IF, Rat)
• Wang Y, Ji N, Gong X et al. Thioredoxin-1 attenuates atherosclerosis development through inhibiting NLRP3 inflammasome Endocrine 2020-06-30 [PMID: 32607763] (WB)
• Tang B, Liu D, Chen L, Liu Y NLRP3 inflammasome inhibitor MCC950 attenuates primary dysmenorrhea in mice via the NF-kappaB/COX-2/PG pathway J Inflamm (Lond) 2020-06-24 [PMID: 32595419] (IF/IHC, Human)
• Zhou X, Liu Z, Ying K et al. WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling Oxid Med Cell Longev 2020-05-30 [PMID: 32566101] (WB, Rat)
• Cornelius DC, Travis OK, Tramel RW et al. NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture PLoS ONE 2020-06-17 [PMID: 32555710] (ICC/IF, Rat)
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.
• Chen R, Yin C, Hu Q et al. Expression profiling of spinal cord dorsal horn in a rat model of complex regional pain syndrome type-I uncovers potential mechanisms mediating pain and neuroinflammation responses J Neuroinflammation 2020-05-23 [PMID: 32446302] (WB, Rat)
• Lalitha N, Sadashivaiah B, Ramaprasad TR, Singh SA Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein PLoS ONE 2020-04-13 [PMID: 32282828] (WB, Rat)
• Arita Y, Yoshinaga Y, Kaneko T et Al. Glyburide inhibits the bone resorption induced by traumatic occlusion in rats J. Periodont. Res. 2020-03-10 [PMID: 32153049] (IF/IHC, Rat)
  
  Details:
  Sprague-Dawley
• Yin, C, Liu, B Et al. Eucalyptol alleviates inflammation and pain responses in a mouse model of gout arthritis. Br J Pharmacol 2020-05-01 [PMID: 31883118] (ICC/IF, Mouse)
• Kim D, Ban K, Lee G, Jun H Lysophosphatidic Acid Induces Podocyte Pyroptosis in Diabetic Nephropathy by an Increase of Egr1 Expression Via Downregulation of EzH2 SSRN Electronic Journal 2023-01-27
• Yadav Sk, Kambis Tn, Kar S et Al. MMP9 mediates acute hyperglycemia-induced human cardiac stem cell death by upregulating apoptosis and pyroptosis in vitro Cell Death Dis 2020-03-13 [PMID: 32170070] (WB)
• Thakur V, Sadanandan J, Chattopadhyay M High-Mobility Group Box 1 Protein Signaling in Painful Diabetic Neuropathy Int J Mol Sci 2020-01-30 [PMID: 32019145] (WB, Rat)
• Huang M, Wang Q, Long F et al. Jmjd3 regulates inflammasome activation and aggravates DSS-induced colitis in mice FASEB J. 2020-01-23 [PMID: 31971317] (WB, Mouse)
• Abraham S, Mencarelli A, Bist P et al. Mobilization of Inflammasome Components forAnaphylactic Degranulation by Mast Cells Research Square 2023-01-31 (ICC/IF, WB, WB, ICC/IF)
• Wei H, Bu R, Yang Q Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway J Diabetes Res 2019-12-09 [PMID: 31886288] (IF/IHC, KD, WB, Mouse)
• Nie S, Wang X, Wang H NLRP3 Inflammasome Mediated Interleukin-1 beta Production in Cancer-Associated Fibroblast Contributes to ALA-PDT for Cutaneous Squamous Cell Carcinoma Cancer Manag Res 2019-12-11 [PMID: 31849516] (WB, Mouse)
• Kar S, Shahshahan HR, Hackfort BT Exercise Training Promotes Cardiac Hydrogen Sulfide Biosynthesis and Mitigates Pyroptosis to Prevent High-Fat Diet-Induced Diabetic Cardiomyopathy Antioxidants (Basel) 2019-12-11 [PMID: 31835893] (WB, Mouse)
• Zhang H, Gong X, Ni S et al. C1q/TNF-related protein-9 attenuates atherosclerosis through AMPK-NLRP3 inflammasome singling pathway Int. Immunopharmacol. 2019-11-11 [PMID: 31727560] (WB, Mouse)
• Chen X, Li H et al. Aerobic Exercise Ameliorates Myocardial Inflammation, Fibrosis and Apoptosis in High-Fat-Diet Rats by Inhibiting P2X7 Purinergic Receptors. Front Physiol 2019-05-11 [PMID: 31681001] (WB, Rat)
• Bao Z, Fan L, Zhao L et al. Silencing of A20 Aggravates Neuronal Death and Inflammation After Traumatic Brain Injury: A Potential Trigger of Necroptosis Front. Mol. Neurosci. 2019-09-19 [PMID: 31607859] (WB, Rat)
• Kron J, Mauro AG, Bonaventura A et al. Inflammasome Formation in Granulomas in Cardiac Sarcoidosis Circ Arrhythm Electrophysiol 2019-09-01 [PMID: 31522533] (Human)
• Yoshida S, Hagiwara Y, Tsuchiya M et al. Involvement of inflammasome activation via elevation of uric acid level in nociception in a mouse model of muscle pain Mol Pain 2019-06-04 [PMID: 31161887] (IF/IHC, Mouse)
• Yang XL, Wang X, Shao L et al. TRPV1 mediates astrocyte activation and interleukin-1 beta release induced by hypoxic ischemia (HI) J Neuroinflammation 2019-05-29 [PMID: 31142341] (IHC-P, ICC/IF, Mouse)
• Zhao X, Ren Y, Lawlor M et al. Involvement of the microglial NLRP3 inflammasome in the anti-inflammatory effect of the antidepressant clomipramine J Affect Disord 2019-05-05 [PMID: 31082627] (WB, Mouse)
• Cornelius DC, Baik CH, Travis OK, et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep. 2019-05-03 [PMID: 31054188] (IM, Rat)
• Chiozzi P, Sarti AC, Sanz JM et al. Amyloid beta-dependent mitochondrial toxicity in mouse microglia requires P2X7 receptor expression and is prevented by nimodipine Sci Rep 2019-04-24 [PMID: 31019207] (WB, Mouse)
• Liu B, Lu R, Li H et al. Zhen-wu-tang ameliorates membranous nephropathy rats through inhibiting NF-kappa B pathway and NLRP3 inflammasome Phytomedicine 2019-04-17 [PMID: 30991182] (IHC-P, Rat)
• Ye, B;Chen, X;Dai, S;Han, J;Liang, X;Lin, S;Cai, X;Huang, Z;Huang, W; Emodin alleviates myocardial ischemia/reperfusion injury by inhibiting gasdermin D-mediated pyroptosis in cardiomyocytes Drug Des Devel Ther 2019-03-25 [PMID: 30988600] (WB, Rat)
• Li J, Liu Y et al. Mechanisms of Aerobic Exercise Upregulating the Expression of Hippocampal Synaptic Plasticity-Associated Proteins in Diabetic Rats. Neural Plast 2019-03-27 [PMID: 30911292] (WB, Rat)
• Doyle TM, Chen Z, Durante M, Salvemini D. Activation of sphingosine-1-phosphate receptor 1 in the spinal cord produces mechano-hypersensitivity through the activation of inflammasome and IL-1b pathway J Pain 2019-02-22 [PMID: 30802544] (WB, Rat)
• Lyu C, Zhang Y, Gu M et al. IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice. Front Cell Neurosci 2018-12-21 [PMID: 30622459] (WB, Mouse)
• Zhang D, Qian J, Zhang P et al. Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro. J. Neurosci. Res. 2019-01-02 [PMID: 30600840] (WB, Rat)
• Hou XX, Dong HR, Sun LJ et al. Purinergic 2X7 Receptor is Involved in the Podocyte Damage of Obesity-Related Glomerulopathy via Activating Nucleotide-Binding and Oligomerization Domain-Like Receptor Protein 3 Inflammasome. Chin. Med. J. 2018-11-20 [PMID: 30425198] (ICC/IF, Mouse)
• Ma X, Wen J, Gao S et al. Paeonia lactiflora Pall. regulates the NF-kB-NLRP3 inflammasome pathway to alleviate cholestasis in rats. J Pharm Pharmacol 2018-09-14 [PMID: 30277564] (WB, Rat)
• Li X, Wang T, Zhang D et al. Andrographolide ameliorates intracerebral hemorrhage induced secondary brain injury by inhibiting neuroinflammation induction. Neuropharmacology 2018-09-12 [PMID: 30218674] (ICC/IF, Rat)
• Fujiwara H, Docampo MD, Riwes M et al. Microbial metabolite sensor GPR43 controls severity of experimental GVHD. Nat Commun 2018-09-10 [PMID: 30201970] (WB, Mouse)
• Li D, Ren W, Jiang Z, Zhu L. Regulation of the NLRP3 inflammasome and macrophage pyroptosis by the p38 MAPK signaling pathway in a mouse model of acute lung injury. Mol Med Rep 2018-08-24 [PMID: 30152849] (ICC/IF, WB, Mouse)
• Li Y, Wu J, Niu Y et al. Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel JPEN J Parenter Enteral Nutr 2018-08-24 [PMID: 30144105] (WB, Rat)
• Jiao H, Rutar M, Fernando N et al. Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration Mol Neurodegener 2018-08-20 [PMID: 30126455] (ICC/IF, Mouse)
• Yichen S, Ruoxi S, Zhouye J et al. Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats Life Sci. 2018-05-22 [PMID: 29800538] (Rat)
• Yang Q, Fu C, Xiao J, Ye Z. Uric acid upregulates the adiponectin-adiponectin receptor 1 pathway in renal proximal tubule epithelial cells. Molecular Medicine Reports 2017-12-19 [PMID: 29359786] (Rat)
• Wu Y, He F et al. Effects of Shizhifang on NLRP3 Inflammasome Activation and Renal Tubular Injury in Hyperuricemic Rats. Evid Based Complement Alternat Med 2018-01-24 [PMID: 29358971] (WB, IF/IHC, Rat)
• Liang Y, Liang S, Zhang Y et al. Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats. Probiotics Antimicrob Proteins 2018-01-20 [PMID: 29353414]
• Kosmidou C, Efstathiou NE, Hoang MV et al. Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration Sci Rep 2018-01-11 [PMID: 29323137] (WB, Mouse)
• Qiu Z, Lei S et al. NLRP3 Inflammasome Activation-Mediated Pyroptosis Aggravates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats. Oxid Med Cell Longev 2017-10-25 [PMID: 29062465] (WB, Rat)
• Szpigel A, Hainault I, Carlier A et al. Lipid environment induces ER stress, TXNIP expression and inflammation in immune cells of individuals with type 2 diabetes. Diabetologia. 2017-10-07 [PMID: 28988346] (Human)
• Wang Y, Yu B, Wang L et al. Pioglitazone ameliorates glomerular NLRP3 inflammasome activation in apolipoprotein E knockout mice with diabetes mellitus PLoS ONE 2017-07-14 [PMID: 28708885] (IHC-P, WB, Mouse)
• Xu X, Chen Y, Song J et al. Mangiferin suppresses endoplasmic reticulum stress in perivascular adipose tissue and prevents insulin resistance in the endothelium. Eur J Nutr. 2017-03-27 [PMID: 28349253] (WB)
• Yin J, Zhao F, Chojnacki JE et al. NLRP3 Inflammasome Inhibitor Ameliorates Amyloid Pathology in a Mouse Model of Alzheimer's Disease. Mol. Neurobiol. 2017-03-02 [PMID: 28255908] (Mouse)
• Beckerman P, Bi-Karchin J, Park AS et al. Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice. Nat. Med. 2017-02-20 [PMID: 28218918]
• Lin C, Chao H, Li Z et al. Omega-3 fatty acids regulate NLRP3 inflammasome activation and prevent behavior deficits after traumatic brain injury Exp Neurol. 2017-04-01 [PMID: 28077337] (IF/IHC, KD, WB, Rat, Human)
• Li M, Li H, Fang F et al. Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms. Neurosci Lett 2017-02-03 [PMID: 28011393] (Mouse)
• Li Y, Zheng JY, Liu JQ et al. Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis. Front Immunol. 2016-12-22 [PMID: 28003810] (IF/IHC, Rat)
• Xiao YD, Huang YY, Wang HX et al. Thioredoxin-interacting protein (TXNIP) mediates NLRP3 inflammasome activation involved in the susceptibility to ischemic acute kidney injury in diabetes Oxid Med Cell Longev 2016-09-19 [PMID: 27867451] (WB, ICC/IF, Human)
• Yeh WJ, Yang HY, Pai MH et al. Long-term administration of advanced glycation end product stimulates the activation of NLRP3 inflammasome and sparking the development of renal injury. The Journal of Nutritional Biochemistry 2016-10-10 [PMID: 27816762] (WB, IF/IHC, Mouse)
• Chen W, Wang J, Luo Y et al. Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue. J Ginseng Res 2016-10-01 [PMID: 27746687]
• Billant O, Leon A, Le Guellec S et al. The dominant-negative interplay between p53, p63 and p73: A family affair. Oncotarget 2016-08-31 [PMID: 27589690] (WB)
• Cinar R, Iyer MR, Liu Z et al. Hybrid inhibitor of peripheral cannabinoid-1 receptors and inducible nitric oxide synthase mitigates liver fibrosis JCI insight 2016-07-21 [PMID: 27525312] (IF/IHC)
• Kadono K, Uchida Y, Hirao H et al. Thrombomodulin attenuates inflammatory damage due to liver ischemia and reperfusion injury in mice in TLR4-dependent manner. Am. J. Transplant. 2016-07-28 [PMID: 27467205] (WB)
• Lampropoulou V, Sergushichev A, Bambouskova M et al. Itaconate Links Inhibition of Succinate Dehydrogenase with Macrophage Metabolic Remodeling and Regulation of Inflammation Cell Metab. 2016-07-12 [PMID: 27374498] (WB, Mouse)
• Li Y, Zhou ZH, Chen MH et al. Inhibition of Mitochondrial Fission and NOX2 Expression Prevent NLRP3 Inflammasome Activation in the Endothelium: The Role of Corosolic Acid Action in the. Antioxid Redox Signal. 2016-03-22 [PMID: 26869350]
• Ge CX, Yu R, Xu MX et al. Betaine prevented fructose-induced NAFLD by regulating LXRa/PPARa pathway and alleviating ER stress in rats. Eur. J. Pharmacol. 2015-11-21 [PMID: 26593707] (WB, Rat)
  
  Details:
  NLRP3/NALP3 was used for WB analysis of liver tissue lysates from Sprague-Dawley rats that were fed with fructose and/or Betaine (a naturally occurring compound in wheat bran, wheat germ, spinach, pretzels, shrimp and wheat bread). Fructose enhanced the expression of NLRP3 while Betaine reversed this effect (Fig. 8E).
• Kim SK, Joe Y, Chen Y et al. Carbon monoxide decreases interleukin-1B levels in the lung through the induction of pyrin. Cell. Mol. Immunol. 2017-04-01 [PMID: 26435068] (WB, Human)
• Lu C, Zhang F, Xu W et al. Curcumin attenuates ethanol-induced hepatic steatosis through modulating Nrf2/FXR signaling in hepatocytes. IUBMB Life. 2015-08-01 [PMID: 26305715] (WB, Rat)
  
  Details:
  NLRP3 antibody was used for WB on lysates of liver tissues from Rats treated with vehicle, ethanol and/or curcumin (Figure 1E). NLRP3 was considered as a proinflammatory signal molecules in this study.
• Li J, Wang Y, Wang Y et al. Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction J. Mol. Cell. Cardiol. 2015-07-18 [PMID: 26196303] (WB, Rat)
• Wang X, Li R, Wang X, Fu Q, Ma. Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome. Neurosci Lett. 2015-07-23 [PMID: 26071904] (Rat)
• Li Y, Yang J, Chen MH et al. Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner. Pharmacol Res. 2015-09-01 [PMID: 26054569]
• Zhao Y, Li Q, Zhao W et al. Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium. J Ethnopharmacol. 2015-07-01 [PMID: 25922268] (WB, Human)
• Li Y, Li J, Li S et al. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol. Appl. Pharmacol. 2015-03-16 [PMID: 25791922] (WB, Mouse)
• Gabrielli E, Pericolini E, Luciano E et al. Aspartyl proteinases of Candida albicans induce caspase-11: implication in promoting inflammatory response Infect. Immun. 2015-02-23 [PMID: 25712931] (WB, Mouse)
  
  Details:
  NLRP3/NALP3 antibody used for WB analysis of lysates from RAW 264.7 cells that were pretreated for 1 hours in the presence or absence of 40 uM IC-11 and then cultured for 30 minutes - 4 hours in the presence or absence of 10 ug LPS with 5 uM ATP, 20ug/ml Sap2 or 20ug/ml Sap6 (Figure 2A).
• Zhang X, Zhang JH, Chen XY et al. ROS-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation Antioxid. Redox Signal. 2015-01-20 [PMID: 25602171] (IF/IHC, Rat)
• Song J, Li J, Hou F et al. Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells. Metab. Clin. Exp. 2014-11-28 [PMID: 25499441] (WB, Human)
• Wu J, Xu X, Li Y et al. Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells. Eur J Pharmacol. 2014-12-15 [PMID: 25446924]
• Joshi S, Wang W, Peck AB, Khan SR. Activation of the NLRP3 Inflammasome in Association with Calcium Oxalate Crystal Induced Reactive Oxygen Species in Kidneys. J Urol. 2015-05-01 [PMID: 25437532] (IHC-P, Rat)
• Chattergoon MA, Latanich R, Quinn J et al. HIV and HCV Activate the Inflammasome in Monocytes and Macrophages via Endosomal Toll-Like Receptors without Induction of Type 1 Interferon. PLoS Pathog. 2014-05-01 [PMID: 24788318] (WB, Human)
  
  Details:
  Primary human monocytes derived from PBMC, Fig S6A. NALP3 was detected at ~120 kDa. The specificity of the NALP3 pAb was validated with NALP3 siRNA.
• Doi T, Doi S, Nakashima A et al. Mizoribine Ameliorates Renal Injury and Hypertension along with the Attenuation of Renal Caspase-1 Expression in Aldosterone-Salt-Treated Rats. PLoS ONE 2014-04-03 [PMID: 24695748] (WB, Rat)
• Serti E, Werner JM, Chattergoon M et al. Monocytes Activate Natural killer Cells via Inflammasome-Induced Interleukin 18 in Response to Hepatitis C Virus Replication. Gastroenterology. 2014-03-28 [PMID: 24685721] (WB, Human)
  
  Details:
  Human PBMC, Fig 6A. NALP3 was detected between the 100 and 120 kDa markers. Note the specificity of the NALP3 pAb was validated with NALP3-siNRA.
• Fan S, Wang Y, Lu J Luteoloside suppresses proliferation and metastasis of hepatocellular carcinoma cells by inhibition of NLRP3 inflammasome PLoS One 2014-02-26 [PMID: 24587153] (WB, Human)
• Fan CY, Wang MX, Ge CX et al. Betaine supplementation protects against high-fructose-induced renal injury in rats. J Nutr Biochem 2013-12-04 [PMID: 24456735] (WB, Rat)
• Pietrella D, Pandey N, Gabrielli E et al. Secreted aspartic proteases of Candida albicans activate the NLRP3 inflammasome. Eur J Immunol. 2013-03-01 [PMID: 23280543] (WB, Human)
  
  Details:
  WB: Fig 5A & B (human primary monocytes), Fig 5C & D (human primary monocytes transfected with siNALP3 or siScrambled Control). Note: The specificity of the NALP3 antibody was validated by WB in human monocytes by siNALP3 knockdown (Fig 5C & D).
• Wang C, Pan Y, Zhang QY, Wang FM, Kong LD. Quercetin and Allopurinol Ameliorate Kidney Injury in STZ-Treated Rats with Regulation of Renal NLRP3 Inflammasome Activation and Lipid Accumulation PLoS One 2012-01-01 [PMID: 22701621] (WB, Rat)

Reviews for NLRP3/NALP3 Antibody (NBP2-12446) (4)

Average Rating: 4

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: anita quon WB Rat 10/05/2020

Summary

• Application: Western Blot
• Sample Tested: Placental tissue
• Species: Rat
• Lot: G

Comments

• Comments: Rat placenta lysates, reduced and denatured; 100 ug total protein resolved on 10% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Blocking with Rockland blocking reagent. Incubate membrane overnight at 4C with 1:500 dilution of NLRP3/NALP3 antibody. Washes with PBS-0.1% tween 20. Incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-rabbit IgG. Images taken with Odyssey V3.0.

reviewed by: Liyong Zhang WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse
• Lot: PO number； E0496223

reviewed by: Verified Customer WB Human 03/26/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human

reviewed by: Verified Customer WB Human 01/29/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human

FAQs for NLRP3/NALP3 Antibody (NBP2-12446). (Showing 1 - 5 of 5 FAQs).

1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
   • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
2. Does NLRP3/NALP3 antibodies come in lyophilized form?
   • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
   • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
   • A sequence corresponding to amino acids 1 - 50 of human NLRP3/NALP3.
5. What epitopes are recognized by IMG-6668A (NBP2-12446) antibody?
   • Unfortunately we don't map the epitopes for any of our antibodies, and thus we don't know where this antibody binds to. However since it is a rabbit polyclonal antibody, we anticipate that the IgG molecules should cover the entire immunogen sequence, spanning from a.a. 1 - 50.

Bioinformatics

• Gene Symbol: NLRP3
• Entrez:
  • 114548(Human)
• Uniprot:
  • Q96P20(Human)