NLRP3/NALP3 Antibody

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Lane A) human NALP3 transfected cell lysate this antibody, B) mouse NALP3 transfected cell lysate this antibody, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/ml.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Image from verified customer review.

Western Blot: NLRP3/NALP3 Antibody [NBP2-12446] - Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NLRP3/NALP3 Antibody Summary

• Immunogen: This antibody was raised against a portion of amino acids 1-50 of human NALP3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 - 5 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
• Application Notes: Use in IHC-P reported in scientific literature (PMID 25437532), IHC (PMID 25602171), ICC (PMID 27867451).
• Theoretical MW: 118 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with New World Monkey. The amino acid sequence used as immunogen is 100% homologous in Human and Monkey and 86% homologous in Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for NLRP3/NALP3 Antibody

• AGTAVPRL
• AII
• AII/AVP
• Angiotensin/vasopressin receptor AII/AVP-like
• anti-mouse NLRP3
• anti-NLRP3
• AVP
• C1orf7
• Caterpiller protein 1.1
• CIAS1
• CLR1.1
• Cold autoinflammatory syndrome 1 protein
• Cold autoinflammatory syndrome 1
• Cryopyrin
• Familial cold autoinflammatory syndrome
• FCAS
• FCU
• Leucine-rich repeat-, and PYD-containing protein 3
• Muckle-Wells syndrome
• MWS
• NACHT
• NACHT, LRR and PYD containing protein 3
• NACHT, LRR and PYD domains-containing protein 3
• NALP3
• NLR family, pyrin domain containing 3
• NLRP3 mouse
• NLRP3 polyclonal
• NLRP3 rat
• NLRP3
• Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
• PYPAF1
• PYRIN-containing APAF1-like protein 1

Background

NALP3 (NACHT, LRR and PYD domains-containing protein 3, or NLRP3) belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. The NALP3 complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NALP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NALP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP3/NALP3 Antibody (NBP2-12446)(39)

Publications using NBP2-12446

• Li Y, Wu J, Niu Y et al. Milk Fat Globule Membrane Inhibits NLRP3 Inflammasome Activation and Enhances Intestinal Barrier Function in a Rat Model of Short Bowel JPEN J Parenter Enteral Nutr Aug 24 2018 [PMID: 30144105] (WB, Rat)
• Jiao H, Rutar M, Fernando N et al. Subretinal macrophages produce classical complement activator C1q leading to the progression of focal retinal degeneration Mol Neurodegener Aug 20 2018 [PMID: 30126455] (ICC/IF, Mouse)
• Yichen S, Ruoxi S, Zhouye J et al. Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats Life Sci. May 22 2018 [PMID: 29800538] (Rat)
• Yang Q, Fu C, Xiao J, Ye Z. Uric acid upregulates the adiponectin-adiponectin receptor 1 pathway in renal proximal tubule epithelial cells. Molecular Medicine Reports 2017 Dec 19 [PMID: 29359786] (Rat)
• Kosmidou C, Efstathiou NE, Hoang MV et al. Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration Sci Rep 2018 Jan 11 [PMID: 29323137] (WB, Mouse)
• Szpigel A, Hainault I, Carlier A et al. Lipid environment induces ER stress, TXNIP expression and inflammation in immune cells of individuals with type 2 diabetes. Diabetologia. 2017 Oct 07 [PMID: 28988346] (Human)
• Wang Y, Yu B, Wang L et al. Pioglitazone ameliorates glomerular NLRP3 inflammasome activation in apolipoprotein E knockout mice with diabetes mellitus PLoS ONE 2017 Jul 14 [PMID: 28708885] (WB, IHC-P, Mouse)
• Xu X, Chen Y, Song J et al. Mangiferin suppresses endoplasmic reticulum stress in perivascular adipose tissue and prevents insulin resistance in the endothelium. Eur J Nutr. Mar 27 2017 [PMID: 28349253] (WB)
• Yin J, Zhao F, Chojnacki JE et al. NLRP3 Inflammasome Inhibitor Ameliorates Amyloid Pathology in a Mouse Model of Alzheimer's Disease. Mol. Neurobiol. Mar 2 2017 [PMID: 28255908] (Mouse)
• Beckerman P, Bi-Karchin J, Park AS et al. Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice. Nat. Med. 2017 Feb 20 [PMID: 28218918]
• Li M, Li H, Fang F et al. Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms. Neurosci Lett 2017 Feb 03 [PMID: 28011393] (Mouse)
• Li Y, Zheng JY, Liu JQ et al. Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis. Front Immunol. Dec 22 2016 [PMID: 28003810] (IHC, Rat)
• Xiao YD, Huang YY, Wang HX et al. Thioredoxin-interacting protein (TXNIP) mediates NLRP3 inflammasome activation involved in the susceptibility to ischemic acute kidney injury in diabetes Oxid Med Cell Longev Sep 19 2016 [PMID: 27867451] (WB, ICC/IF, Human)
• Yeh WJ, Yang HY, Pai MH et al. Long-term administration of advanced glycation end product stimulates the activation of NLRP3 inflammasome and sparking the development of renal injury. The Journal of Nutritional Biochemistry Oct 10 2016 [PMID: 27816762] (WB, IHC, Mouse)
• Chen W, Wang J, Luo Y et al. Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue. J Ginseng Res 2016 Oct [PMID: 27746687]
• Billant O, Leon A, Le Guellec S et al. The dominant-negative interplay between p53, p63 and p73: A family affair. Oncotarget Aug 31 2016 [PMID: 27589690] (WB)
• Cinar R, Iyer MR, Liu Z et al. Hybrid inhibitor of peripheral cannabinoid-1 receptors and inducible nitric oxide synthase mitigates liver fibrosis JCI insight 2016 Jul 21 [PMID: 27525312] (IHC)
• Kadono K, Uchida Y, Hirao H et al. Thrombomodulin attenuates inflammatory damage due to liver ischemia and reperfusion injury in mice in TLR4-dependent manner. Am. J. Transplant. Jul 28 2016 [PMID: 27467205] (WB)
• Lampropoulou V, Sergushichev A, Bambouskova M et al. Itaconate Links Inhibition of Succinate Dehydrogenase with Macrophage Metabolic Remodeling and Regulation of Inflammation Cell Metab. 2016 Jul 12 [PMID: 27374498] (WB, Mouse)
• Li Y, Zhou ZH, Chen MH et al. Inhibition of Mitochondrial Fission and NOX2 Expression Prevent NLRP3 Inflammasome Activation in the Endothelium: The Role of Corosolic Acid Action in the. Antioxid Redox Signal. 2016 Mar 22 [PMID: 26869350]
• Ge CX, Yu R, Xu MX et al. Betaine prevented fructose-induced NAFLD by regulating LXRa/PPARa pathway and alleviating ER stress in rats. Eur. J. Pharmacol. 2015 Nov 21 [PMID: 26593707] (WB, Rat)
  
  Details:
  NLRP3/NALP3 was used for WB analysis of liver tissue lysates from Sprague-Dawley rats that were fed with fructose and/or Betaine (a naturally occurring compound in wheat bran, wheat germ, spinach, pretzels, shrimp and wheat bread). Fructose enhanced the expression of NLRP3 while Betaine reversed this effect (Fig. 8E).
• Kim SK, Joe Y, Chen Y et al. Carbon monoxide decreases interleukin-1B levels in the lung through the induction of pyrin. Cell. Mol. Immunol. Apr 1 2017 [PMID: 26435068] (WB, Human)
• Lu C, Zhang F, Xu W et al. Curcumin attenuates ethanol-induced hepatic steatosis through modulating Nrf2/FXR signaling in hepatocytes. IUBMB Life. 2015 Aug 01 [PMID: 26305715] (WB, Rat)
  
  Details:
  NLRP3 antibody was used for WB on lysates of liver tissues from Rats treated with vehicle, ethanol and/or curcumin (Figure 1E). NLRP3 was considered as a proinflammatory signal molecules in this study.
• Li J, Wang Y, Wang Y et al. Pharmacological activation of AMPK prevents Drp1-mediated mitochondrial fission and alleviates endoplasmic reticulum stress-associated endothelial dysfunction J. Mol. Cell. Cardiol. 2015 Jul 18 [PMID: 26196303] (WB, Rat)
• Wang X, Li R, Wang X et al. Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome. Neurosci Lett 2015 Jul 23 [PMID: 26071904] (Rat)
• Li Y, Yang J, Chen MH et al. Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner. Pharmacol Res 2015 Jun 6 [PMID: 26054569]
• Zhao Y, Li Q, Zhao W et al. Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium J Ethnopharmacol 2015 Apr 25 [PMID: 25922268] (WB, Human)
• Li Y, Li J, Li S et al. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol. Appl. Pharmacol. 2015 Mar 16 [PMID: 25791922] (WB, Mouse)
• Gabrielli E, Pericolini E, Luciano E et al. Aspartyl proteinases of Candida albicans induce caspase-11: implication in promoting inflammatory response Infect. Immun. 2015 Feb 23 [PMID: 25712931] (WB, Mouse)
  
  Details:
  NLRP3/NALP3 antibody used for WB analysis of lysates from RAW 264.7 cells that were pretreated for 1 hours in the presence or absence of 40 uM IC-11 and then cultured for 30 minutes - 4 hours in the presence or absence of 10 ug LPS with 5 uM ATP, 20ug/ml Sap2 or 20ug/ml Sap6 (Figure 2A).
• Zhang X, Zhang JH, Chen XY et al. ROS-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation Antioxid. Redox Signal. 2015 Jan 20 [PMID: 25602171] (IHC, Rat)
• Song J, Li J, Hou F et al. Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells. Metab. Clin. Exp. 2014 Nov 28 [PMID: 25499441] (WB, Human)
• Wu Jianjun, Xu Xiaoshan, Li Yi et al. Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells. Eur J Pharmacol. 2014 Dec 15 [PMID: 25446924]
• Joshi S, Wang W, Peck AB, Khan SR. Activation of the NLRP3 Inflammasome in association with Calcium Oxalate crystal-induced Reactive Oxygen Species in kidneys. J. Urol. 2014 Nov 28 [PMID: 25437532] (IHC-P, Rat)
  
  Details:
  NLRP3/NALP3 antibody used for IHC-P on kidneys from male rats that were given hydroxy-l-proline to induce hyperoxaluria followed by apocynin (an antioxidant and nonspecific inhibitor of NADPH oxidase) - formalin fixation, paraffin embedding, xylene-ethanol processing, overnight 4C primary antibody incubation, rabbit IgG isotype controls (Figure 3)
• Chattergoon MA, Latanich R, Quinn J et al. HIV and HCV Activate the Inflammasome in Monocytes and Macrophages via Endosomal Toll-Like Receptors without Induction of Type 1 Interferon. PLoS Pathog. 2014 May 01 [PMID: 24788318] (WB, Human)
  
  Details:
  Primary human monocytes derived from PBMC, Fig S6A. NALP3 was detected at ~120 kDa. The specificity of the NALP3 pAb was validated with NALP3 siRNA.
• Doi T, Doi S, Nakashima A et al. Mizoribine Ameliorates Renal Injury and Hypertension along with the Attenuation of Renal Caspase-1 Expression in Aldosterone-Salt-Treated Rats. PLoS ONE 2014 Apr 03 [PMID: 24695748] (WB, Rat)
• Serti E, Werner JM, Chattergoon M et al. Monocytes Activate Natural killer Cells via Inflammasome-Induced Interleukin 18 in Response to Hepatitis C Virus Replication. Gastroenterology. 2014 Mar 28 [PMID: 24685721] (WB, Human)
  
  Details:
  Human PBMC, Fig 6A. NALP3 was detected between the 100 and 120 kDa markers. Note the specificity of the NALP3 pAb was validated with NALP3-siNRA.
• Fan CY, Wang MX, Ge CX et al. Betaine supplementation protects against high-fructose-induced renal injury in rats. J Nutr Biochem 2013 Dec 4 [PMID: 24456735] (WB, Rat)
• Pietrella D, Pandey N, Gabrielli E et al. Secreted aspartic proteases of Candida albicans activate the NLRP3 inflammasome. Eur J Immunol. 2013 Mar [PMID: 23280543] (WB, Human)
  
  Details:
  WB: Fig 5A & B (human primary monocytes), Fig 5C & D (human primary monocytes transfected with siNALP3 or siScrambled Control). Note: The specificity of the NALP3 antibody was validated by WB in human monocytes by siNALP3 knockdown (Fig 5C & D).
• Wang C, Pan Y, Zhang QY, Wang FM, Kong LD. Quercetin and Allopurinol Ameliorate Kidney Injury in STZ-Treated Rats with Regulation of Renal NLRP3 Inflammasome Activation and Lipid Accumulation PLoS One 2012 [PMID: 22701621] (WB, Rat)

Reviews for NLRP3/NALP3 Antibody (NBP2-12446) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous WB Mouse 08/06/2016

Summary

• Application: Western Blot
• Species: Mouse
• Lot: PO number； E0496223
• File: View PDF

reviewed by: Chunhui Sun WB Human 03/26/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human

Blocking

• Blocking Details: BSA RT 1h

Details

• Detection Notes: ECL

reviewed by: Chunhui Sun WB Human 01/29/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human

Blocking

• Blocking Details: BSA, 5%, 1 hour, RT

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG
• Secondary Concentration: 1:5000

Details

• Detection Notes: ECL

FAQs for NLRP3/NALP3 Antibody (NBP2-12446). (Showing 1 - 5 of 5 FAQs).

1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
   • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
2. Does NLRP3/NALP3 antibodies come in lyophilized form?
   • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
   • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
   • A sequence corresponding to amino acids 1 - 50 of human NLRP3/NALP3.
5. What epitopes are recognized by IMG-6668A (NBP2-12446) antibody?
   • Unfortunately we don't map the epitopes for any of our antibodies, and thus we don't know where this antibody binds to. However since it is a rabbit polyclonal antibody, we anticipate that the IgG molecules should cover the entire immunogen sequence, spanning from a.a. 1 - 50.

Bioinformatics

• Gene Symbol: NLRP3
• Entrez:
  • 114548(Human)
  • 216799(Mouse)
  • 287362(Rat)
• Uniprot:
  • Q96P20(Human)