p62/SQSTM1 Antibody (1478)

Images

 
Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p62/SQSTM1 antibody (1478) diluted at 1:500. HRP-conjugated ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of Samples: HepG2 cells treated with 3uM thapsigargin 16 hrs (rigtht) and mock (left) were fixed ...read more
Immunohistochemistry-Frozen: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Frozen sectioned adult mouse retina. Green: SQSTM1 protein stained by SQSTM1 antibody [1478] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by ...read more
Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - An intracellular stain was performed on SK-MEL-28 cells with p62/SQSTM1 Antibody (1478) NBP2-43663APC (blue) and a matched isotype control (orange). Cells were ...read more
Biological Strategies: Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hr and 24hr. HepG2 whole cell extracts (30 ug) were ...read more
Biological Strategies: Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hr and 24hr. Huh-7 whole cell extracts (30 ug) were ...read more
Genetic Strategies: Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed ...read more
Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of HeLa cell. Brown: Unlabelled sample was also used as a control. Blue: SQSTM1 antibody [1478] dilution: 1:100.
Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - An intracellular stain was performed on U-937 cells with p62/SQSTM1 Antibody (1478) NBP2-43663AF647 (blue) and a matched isotype control (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, KO
Clone
1478
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

p62/SQSTM1 Antibody (1478) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SQSTM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH7, 20% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for p62/SQSTM1 Antibody (1478)

  • A170
  • Autophagy Receptor P62
  • DMRV
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • FTDALS3
  • NADGP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

p62/SQSTM1 (ubiquitin-binding protein p62/Sequestrosome-1) is an intracellular protein (theoretical molecular weight 47.7 kDa) which localizes to the cytoplasm, nucleus, autophagosomes and lysosomes in all tissues (1). Structurally, p62/SQSTM1 consists of multiple domains (e.g., Phox1 and Bem1p-PB1, zinc finger-ZZ, TRAF6 binding domain-TB, Keap1-interacting region-KIR, LC3 interacting region-LIR and ubiquitin-associated domain-UBA) for interaction with various protein targets. Additional domains include nuclear export (NES) and nuclear localization signals (NLS1 and NLS2). p62/SQSTM1 is a multifunctional scaffold protein which forms oligomers with itself or with other proteins through interactions with PB1 domains.

Abnormal function of p62/SQSTM1 is associated with a range of disease states such as neurodegeneration, cancer, and metabolic disorders (2). Mutations in the p62/SQSTM1 sequence have been linked to Paget's disease of the bone, amyotrophic lateral sclerosis, and frontotemporal lobar degeneration. In Parkinson's disease, p62/SQSTM1 has been linked to microglia activation and subsequent neuroinflammation (3). Functionally, p62/SQSTM1 is involved in a broad range of cellular processes such as amino acid sensing by interaction with mTORC1, oxidative stress response through interaction with Keap1, and targeting cargo for autophagy by interacting with ubiquitin labeled proteins (1).

To induce selective autophagy, p62/SQSTM1 forms long oligomers or helical filaments which interact with LC3 and ubiquitin labeled proteins and lead to the initiation of the autophagosome formation (2). p62/SQSTM1 is not only a selective autophagy receptor but also an autophagy substrate, as its engulfed by the autophagosome and degraded by the autophagolysosome. Monitoring LC3 levels is the standard for assessing autophagic flux, however monitoring p62/SQSTM1 levels by Western blot in the presence and absence of autophagy inhibitors (e.g., Chloroquine) is also a common practice (4). Besides its activity as a selective autophagy receptor, p62/SQSTM1 also plays a role as an adaptor in signaling cascades leading to NFkB activation downstream of TNF-R, IL-1 beta R, TrkA and p75NTR. Briefly, for NFkB signaling downstream of the TNF-R activation, p62/SQSTM1 engages RIP1 kinase and PCK lambda/iota through the ZZ and PB1 domains, respectively (5).

References

1.Katsuragi, Y., Ichimura, Y., & Komatsu, M. (2015). P62/SQSTM1 functions as a signaling hub and an autophagy adaptor. FEBS Journal. https://doi.org/10.1111/febs.13540

2. Sanchez-Martin, P., & Komatsu, M. (2018). p62/SQSTM1 - Steering the cell through health and disease. Journal of Cell Science. https://doi.org/10.1242/jcs.222836

3. Yao, L., Zhu, Z., Wu, J., Zhang, Y., Zhang, H., Sun, X., ... Lu, G. (2019). MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease. The FASEB Journal. https://doi.org/10.1096/fj.201900363r

4 Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Arozena, A. A., ... Zughaier, S. M. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. https://doi.org/10.1080/15548627.2015.1100356

5. Bitto, A., Lerner, C. A., Nacarelli, T., Crowe, E., Torres, C., & Sell, C. (2014). p62/SQSTM1 at the interface of aging, autophagy, and disease. Age. https://doi.org/10.1007/s11357-014-9626-3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SQSTM1