This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Partial Recombinant Protein
>80% by SDS-PAGE and Coomassie blue staining
37.84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using H00004843-Q01 in the following application:
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
>80% by SDS-PAGE and Coomassie blue staining
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human iNOS GST (N-Term) Protein
Inducible NO synthase
nitric oxide synthase 2, inducible
nitric oxide synthase 2A (inducible, hepatocytes)
nitric oxide synthase, inducible
nitric oxide synthase, macrophage
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).
The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).
1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489
2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174
3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
Cells were blocked with 5% milk in PBST 1 hour at RT
Used anti-iNOS antibody from Abcam, diluted 1/500 in TBST, incubated for an hour at RT
goat anti-rabbit HRP diluted 1/2000 in TBST
Secondary Manufacturer Cat#
Used Western blotting reagents and film to detect. Film was exposed for 2 minutes.
We got 1 dark band at the appropriate size for this iNOS recombinant protein when using it as positive control in Western blotting assay. We got another band at around 25kD but it was faint. For our samples, we got 2 bands around 45kD, each on one side of the band for the iNOS protein. Still not sure what it means but I think this protein worked well in the assay anyhow...
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FAQs for iNOS Partial Recombinant Protein (H00004843-Q01). (Showing 1 - 1 of 1 FAQ).
We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
iNOS: The Nitric Oxide Boss Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti... Read full blog post.
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