Cells were blocked with 5% milk in PBST 1 hour at RT
Used anti-iNOS antibody from Abcam, diluted 1/500 in TBST, incubated for an hour at RT
goat anti-rabbit HRP diluted 1/2000 in TBST
Secondary Manufacturer Cat#
Used Western blotting reagents and film to detect. Film was exposed for 2 minutes.
We got 1 dark band at the appropriate size for this iNOS recombinant protein when using it as positive control in Western blotting assay. We got another band at around 25kD but it was faint. For our samples, we got 2 bands around 45kD, each on one side of the band for the iNOS protein. Still not sure what it means but I think this protein worked well in the assay anyhow...
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FAQs for iNOS Partial Recombinant Protein (H00004843-Q01). (Showing 1 - 1 of 1 FAQ).
We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
Additional iNOS Partial Recombinant Protein Products
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