iNOS Antibody

Product Discontinued

iNOS Antibody Summary

• Immunogen: iNOS Antibody was made to a synthetic peptide derived from the C-terminus of mouse iNOS and nNOS.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot
• Application Notes: Use in FLOW reported in scientific literature (PMID: 26857066). Use in ICC/IF reported in scientific literature (PMID: 30158522).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for iNOS Antibody

• EC 1.14.13.39
• Hepatocyte NOS
• HEP-NOS
• Inducible NO synthase
• Inducible NOS
• iNOS
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, inducible
• nitric oxide synthase, macrophage
• NOS type II
• NOS
• NOS2
• NOS2A
• Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for iNOS Antibody (NBP1-50606)(6)

Publications using NBP1-50606

• Oizumi H, Miyamoto Y, Seiwa C et al. Lethal Adulthood Myelin Breakdown by Oligodendrocyte-specific Ddx54 Knockout iScience 2023-07-01 [PMID: 37720086] (ICC/IF, Mouse)
• Fitzpatrick Sf, Lambden S, Macias D et Al. 2-Hydroxyglutarate Metabolism Is Altered in an in vivo Model of LPS Induced Endotoxemia Front Physiol 2020-03-03 [PMID: 32194434] (WB, Human)
• Schroter D, Neugart S, Schreiner M et al. Amaranth's 2-Caffeoylisocitric Acid-An Anti-Inflammatory Caffeic Acid Derivative That Impairs NF-kB Signaling in LPS-Challenged RAW 264.7 Macrophages Nutrients 2019-03-07 [PMID: 30866427] (Mouse)
• Baruteau J, Perocheau D, Hanley J et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer Nat Commun. [PMID: 30158522] (IF/IHC, ICC/IF, Mouse)
• Kanazawa H, Tseliou E, Dawkins JF et al. Durable Benefits of Cellular Postconditioning: Long-Term Effects of Allogeneic Cardiosphere-Derived Cells Infused After Reperfusion in Pigs with Acute Myocardial Infarction. J Am Heart Assoc 2016-02-08 [PMID: 26857066] (FLOW)
• Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015-01-08 [PMID: 25567297] (IHC-P, Rat)
  
  Details:
  iNOS antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group as well as Dunaliella-treated/DS groups of rats showed severe immunoreactiivity of iNOS in mucosa, submucosa and muscularis, whereas, the control as well as the ones treated with SP or SSZ had weak to moderate iNOS expression. (Figure 6). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.

FAQs for iNOS Antibody (NBP1-50606). (Showing 1 - 2 of 2 FAQs).

1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
   • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
2. wondering if you could tell me the exact protein concentration for the NBP1-50606-0.25ml Rabbit anti iNOS antibody? Lot number is 73084.
   • In regards to your inquiry about this iNOS Antibody NBP1-50606 lot 73084, the concentration is 0.38mg/ml.

Bioinformatics

• Gene Symbol: NOS2