iNOS Antibody

Images

 
Immunohistochemistry-Paraffin: iNOS Antibody [NBP1-50606] - Human adrenal formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval concentration 5 ug/ml.
Immunohistochemistry-Paraffin: iNOS Antibody [NBP1-50606] - Human spleen formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval concentration 5 ug/ml.
Immunohistochemistry-Paraffin: iNOS Antibody [NBP1-50606] - Human testis formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval concentration 5 ug/ml.
Immunohistochemistry-Paraffin: iNOS Antibody [NBP1-50606] - Human pancreas, nerve formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval concentration 5 ug/ml.
Immunohistochemistry-Paraffin: iNOS Antibody [NBP1-50606] - Human prostate, nerve formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

iNOS Antibody Summary

Immunogen
A synthetic peptide derived from the C-terminus of mouse iNOS and nNOS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Use in FLOW reported in scientific literature (PMID: 26857066). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30158522).
Publications
Read Publications using
NBP1-50606 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and BSA
Preservative
Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for iNOS Antibody

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-P

Publications for iNOS Antibody (NBP1-50606)(5)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, IHC-P.


Filter By Application
FLOW
(1)
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
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Filter By Species
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 5 of 5.
Publications using NBP1-50606 Applications Species
Schroter D, Neugart S, Schreiner M et al. Amaranth's 2-Caffeoylisocitric Acid-An Anti-Inflammatory Caffeic Acid Derivative That Impairs NF-kB Signaling in LPS-Challenged RAW 264.7 Macrophages Nutrients Mar 7 2019 [PMID: 30866427] (Mouse) Mouse
Baruteau J, Perocheau D, Hanley J et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer bioRxiv Jun 15 2018 [PMID: 30158522] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Baruteau J, Perocheau D, Hanley J et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer Nat Commun Jun 15 2018 [PMID: 30158522] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Kanazawa H, Tseliou E, Dawkins JF et al. Durable Benefits of Cellular Postconditioning: Long-Term Effects of Allogeneic Cardiosphere-Derived Cells Infused After Reperfusion in Pigs with Acute Myocardial Infarction. J Am Heart Assoc 2016 Feb 08 [PMID: 26857066] (FLOW) FLOW
Abdel-Daim MM, Farouk SM, Madkour FF, Azab SS. Anti-inflammatory and immunomodulatory effects of Spirulina platensis in comparison to Dunaliella salina in acetic acid-induced rat experimental colitis Immunopharmacol Immunotoxicol. 2015 Jan 08 [PMID: 25567297] (IHC-P, Rat)

Details:
iNOS antibody used for IHC-P on sections of colonic lesions from Rats subjected to acetic acid-induced experimental colitis followed by treatment or not with Spirulina platensis/SP, Dunaliella salina/DS extracts or Sulfasalazine/SSZ (standard drug used to treat bowel inflammation, diarrhea, rectal bleeding, and abdominal pain in patients with ulcerative colitis). The acetic acid-treated group as well as Dunaliella-treated/DS groups of rats showed severe immunoreactiivity of iNOS in mucosa, submucosa and muscularis, whereas, the control as well as the ones treated with SP or SSZ had weak to moderate iNOS expression. (Figure 6). Spirulina platensis exerted superior beneficial effects in alleviating acetic acid-induced colitis via anti-inflammatory actions which were even better than SSZ.
IHC-P Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for iNOS Antibody (NBP1-50606). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. wondering if you could tell me the exact protein concentration for the NBP1-50606-0.25ml Rabbit anti iNOS antibody ?lot number is 73084.
    • In regards to your inquiry about this iNOS Antibody NBP1-50606 lot 73084. The concentration is 0.38mg/ml.

Secondary Antibodies

 

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol NOS2