FXR/NR1H4 Antibody - Azide Free

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody- Mouse tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin-NBP2-16550-FXR/NR1H4 Antibody-Rat tissues.NR1H4 stained by NR1H4 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Biochemical measurements & the mRNA & protein expression of top 5 NRs. A, Results of biochemical parameter measurements. Among the serum indicators, ALT in HFD group higher than it in NC group (P = .0004) & CSP group (P = .0222); the AST in HFD group higher than it in NC group (P = .0006) & CSP group (P = .0149); ALP in HFD group higher than it in NC group (P < .0001) & CSP group (P = .0013), & it lower in NC group than in CSP group (P = .0017); in HFD group, the TC level higher than it in NC group (P < .0001) & CSP group (P = .0003), & in NC group, it lower than it in CSP group (P < .0001); in HFD group, the TG level higher than it in NC group (P = .0011) & CSP group (P = .0476); in HFD group, the GLU level higher than it in NC group (P < .0001) & CSP group (P = .0002); & in HFD group, the HDL‐c level higher than it in NC group (P < .0001) & CSP group (P = .0022), & in NC group, it lower than it in CSP group (P < .0001) (n = 10). Among the liver indicators, in NC group, the TC level lower than it in HFD group (P = .0276) & CSP group (P = .0427); in NC group, the TG level lower than it in HFD group (P = .0427) & CSP group (P = .0448) (n = 6). B, The mRNA expression of top 5 NRs in liver. PPAR gamma in HFD group expressed at a higher level than it in NC group (P = .0072) & CSP group (P = .0489); FXR in HFD group expressed at a lower level than it in NC group (P = .0222) & CSP group (P = .0331); PPAR alpha in HFD group expressed lower than NC group (P = .0143) & CSP group (P = .0082); & RAR alpha in HFD group expressed at a higher level than it in NC group (P = .0001) & CSP group (P = .0012) (n = 10). C, Expression of top 5 NRs in liver measured by automatic WB analysis Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32189432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

FXR/NR1H4 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit FXR/NR1H4 Antibody - Azide Free (NBP2-16550) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-FXR/NR1H4 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FXR/NR1H4. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Porcine (92%), Bovine (94%), Rabbit (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1H4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Simple Western
  • Western Blot 1:500-1:3000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: separated by Size
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FXR/NR1H4 Antibody - Azide Free

• BAR
• bile acid receptor
• farnesoid X receptor
• Farnesoid X-activated receptor
• Farnesol receptor HRR-1
• FXR
• FXRBAR
• HRR-1
• HRR1MGC163445
• NR1H4
• Nuclear receptor subfamily 1 group H member 4
• nuclear receptor subfamily 1, group H, member 4
• Retinoid X receptor-interacting protein 14
• RIP14
• RIP14RXR-interacting protein 14

Background

FXR and LXR fall into a category of proteins termed orphan receptors; because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, as well as several novel receptors including LXR (also referred to as RLD-1) and FXR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FXR/NR1H4 Antibody (NBP2-16550)(6)

Publications using NBP2-16550

• Xue C, Zeng P, Gong K et Al. Nogo-B inhibition facilitates cholesterol metabolism to reduce hypercholesterolemia Cell Rep 2024-09-03 [PMID: 39235944]
• Jinjing Chen, Ruoyu Wang, Feng Xiong, Hao Sun, Byron Kemper, Wenbo Li, Jongsook Kemper, Pramod K Mistry, Pramod K Mistry Hammerhead-type FXR agonists induce an enhancer RNA Fincor that ameliorates nonalcoholic steatohepatitis in mice eLife 2024-04-15 [PMID: 38619504]
• Wang X, Yang Y, Chen Y et al. MEK1/2 inhibitors induce class I alcohol dehydrogenase (ADH1) expression by regulating farnesoid X receptor in hepatic cell lines and C57BL/6J mouse Molecular biology reports 2022-03-25 [PMID: 35338439]
• Nie H, Deng Y, Zheng C et Al. A network pharmacology-based approach to explore the effects of Chaihu Shugan powder on a non-alcoholic fatty liver rat model through nuclear receptors J. Cell. Mol. Med. 2020-03-18 [PMID: 32189432] (Simple Western, Rat)
• Zhao Q, Liu F, Cheng Y et al. Celastrol protects from cholestatic liver injury though modulation of SIRT1-FXR signaling. Mol. Cell Proteomics 2019-01-07 [PMID: 30617157] (WB, Mouse)
• Lian F, Wang Y, Xiao Y et al. Activated farnesoid X receptor attenuates apoptosis and liver injury in autoimmune hepatitis. Mol Med Rep 2015-10-01 [PMID: 26238153] (WB)

Bioinformatics

• Gene Symbol: NR1H4