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FIH-1/HIF-1AN Antibody

Images

 
Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Analysis of astrocytes lysate from Rat brain using FIH-1/HIF-1AN antibody at 1:500 dilution with ECL detection. The antibody detected a major band at ~40 kDa position ...read more
Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Representative picture of western blot in histopathologically unchanged tissue (N) and primary cancerous tissue (C) from patients with CRC. Immunodetection of bands was ...read more
Immunohistochemistry: FIH-1/HIF-1AN Antibody [NB100-428] - Detection of FIH-1 in rat brain tissue.
Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Rat VSMC (vascular smoot muscle) cells treated (siFIH) or not (CTL) with a specific siRNA against FIH-1. Western blot image submitted by a verified customer review.
Biological Strategies: Knockdown Validated: FIH-1/HIF-1AN Antibody [NB100-428] - (C) Cytoplasmic FIH-1 (lower band) and MMP14 expression levels in both aerobic and hypoxic conditions in the 3 cell lines. (E) ...read more
Lysyl Oxidase (LOX) expression in malignant and non-malignant human prostate tissue.(A) Representative immunohistochemical staining of LOX (brown) in sections of non-malignant prostate tissue specimens (TINT epithelium ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, MS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FIH-1/HIF-1AN Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIF1AN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated reported in scientific literature (PMID 31937588)
  • Western Blot 1:1000-1:5000
Theoretical MW
40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FIH-1/HIF-1AN Overexpression Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate
Control Peptide
FIH-1/HIF-1AN Peptide (NB100-428PEP)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-428 in the following applications:

Publications
Read Publications using
NB100-428 in the following applications:

  • EM
    1 publication
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • KO
    1 publication
  • WB
    14 publications

Reactivity Notes

Human, rat and mouse (PMID 19720742).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FIH-1/HIF-1AN Antibody

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor inhibiting HIF1 (FIH1) is an asparaginyl hydroxylase enzyme that regulates HIF's transcriptional activity by hydroxylating HIF-1 alpha at 'Asp-803' in the CAD (C-terminal transactivation domain). Hydroxylation via PHDs followed by proteosome degradation is another mechanism that regulates HIF1, however, FIH1 is able to exercise the control even under severe hypoxic conditions, when PHD enzymes fail to do so. Localized mainly in cytoplasm, FIH1 functions as oxygen sensor and FIH1 interaction with NOTCH1 results in its nuclear localization whereas FIH1-ABPA3 interaction leads to its perinuclear localization. During normoxic conditions, FIH1 hydroxylates a conserved asparaginyl residue within CTAD of HIF-1 alpha/HIF-2 alpha, leading to steric clash that prevents the recruitment of the co-activators p300 and CBP. On the other hand, under hypoxic conditions, FIH1 is inactive, which results in activation of HIF-alpha signaling. FIH1 is involved in transcriptional repression through interaction with HIF1A, VHL and HDAC and hydroxylates specific Asn residues within ARD (ankyrin repeat domains) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A as well as other ARD-containing proteins. FIH1 also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. FIH1 has the ability to negatively regulate NOTCH1 activity thus accelerating myogenic differentiation, and positively regulates ASB4 activity which promotes vascular differentiation. FIH1-Mint3 binding affects FIH1's ability to modify HIF-1 alpha in an oxygen-independent manner and FIH1-Bax interaction plays inhibitory role in apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-428
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP, MS, KD, KO

Publications for FIH-1/HIF-1AN Antibody (NB100-428)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, ICC/IF, IHC-P, IP, KO, WB.


Filter By Application
EM
(1)
ICC/IF
(1)
IHC-P
(1)
IP
(1)
KO
(1)
WB
(14)
All Applications
Filter By Species
Human
(10)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-428 Applications Species
E G, Sun B, Liu B et al. Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Brain research bulletin 2022-11-08 [PMID: 36334804] (WB, Human)

Details:
Dilution used in WB 1:1000
WB Human
Li ZL, Wang B, Lv LL et al. FIH-1-modulated HIF-1 alpha C-TAD promotes acute kidney injury to chronic kidney disease progression via regulating KLF5 signaling Acta pharmacologica Sinica 2021-03-03 [PMID: 33658705]
Chung Y, Saitoh Y, Hayashi T et al. Mint3 is dispensable for pancreatic and kidney functions in mice Biochemistry and biophysics reports 2020-12-01 [PMID: 33319072] (WB) WB
Mader J, Huber J, Bonn F et al. Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin binding J. Biol. Chem. 2020-01-14 [PMID: 31937588] (WB, KO, Human) WB, KO Human
Docherty C K, Nilsen M et al. Influence of 2-Methoxyestradiol and Sex on Hypoxia-Induced Pulmonary Hypertension and Hypoxia-Inducible Factor-1-alpha. J Am Heart Assoc 2019-05-03 [PMID: 30819028] (WB, Human) WB Human
Pillai MR, Mihi B, Ishiwata K et al. Myc-induced nuclear antigen constrains a latent intestinal epithelial cell-intrinsic anthelmintic pathway PLoS ONE 2019-02-26 [PMID: 30807587] (WB, Mouse) WB Mouse
Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016-05-31 [PMID: 27245613] (WB, Human) WB Human
Nakaoka HJ, Hara T, Yoshino S et al. NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells Sci Rep 2016-03-07 [PMID: 26948053] (IP, Human) IP Human
Kiriakidis S, Henze AT, Kruszynska-Ziaja I et al. Factor-inhibiting HIF-1 (FIH-1) is required for human vascular endothelial cell survival FASEB J. 2015-04-02 [PMID: 25837583] (WB, Human) WB Human
Karttunen S, Duffield M, Scrimgeour Nr et al. Oxygen-dependent hydroxylation by Factor Inhibiting HIF (FIH) regulates the TRPV3 ion channel. J. Cell. Sci. 2014-11-20 [PMID: 25413349] (WB) WB
Show All 26 Publications.

Reviews for FIH-1/HIF-1AN Antibody (NB100-428) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.

Reviews using NB100-428:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FIH-1/HIF-1AN NB100-428
Enlarge
5
reviewed by:
Darren Richard
WB Rat 06/05/2020
View

Summary

ApplicationWestern Blot
Sample TestedVascular Smooth Muscle Cells,Whole cell lysate
SpeciesRat
LotA3
Western Blot FIH-1/HIF-1AN NB100-428
Enlarge
4
reviewed by:
Verified Customer
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedCell lysates from prostate cancer cell lines
SpeciesHuman
LotA2

Comments

CommentsSample: Whole cell lysates of prostate cancer cell lines (20ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
  4
reviewed by:
Verified Customer
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22277691
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NB100-428) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Darren Richard
06/05/2020
Application: WB
Species: Rat

Verified Customer
11/12/2017
Application: WB
Species: Human

 
Verified Customer
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIF1AN
Entrez
Uniprot