FIH-1/HIF-1AN Antibody

Images

 
Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Analysis of astrocytes lysate from Rat brain using FIH-1/HIF-1AN antibody at 1:500 dilution with ECL detection. The antibody detected a major band at ~40 kDa position ...read more
Immunohistochemistry: FIH-1/HIF-1AN Antibody [NB100-428] - Detection of FIH-1 in rat brain tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FIH-1/HIF-1AN Antibody Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIF1AN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FIH-1/HIF-1AN Lysate (NBL1-11541)
Control Peptide
FIH-1/HIF-1AN Peptide (NB100-428PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-428 in the following applications:

Publications
Read Publications using
NB100-428 in the following applications:

  • EM
    1 publication
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    9 publications

Reactivity Notes

Human, rat and mouse (PMID 19720742).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FIH-1/HIF-1AN Antibody

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor inhibiting HIF1 (FIH1) is an asparaginyl hydroxylase enzyme that regulates HIF's transcriptional activity by hydroxylating HIF-1 alpha at 'Asp-803' in the CAD (C-terminal transactivation domain). Hydroxylation via PHDs followed by proteosome degradation is another mechanism that regulates HIF1, however, FIH1 is able to exercise the control even under severe hypoxic conditions, when PHD enzymes fail to do so. Localized mainly in cytoplasm, FIH1 functions as oxygen sensor and FIH1 interaction with NOTCH1 results in its nuclear localization whereas FIH1-ABPA3 interaction leads to its perinuclear localization. During normoxic conditions, FIH1 hydroxylates a conserved asparaginyl residue within CTAD of HIF-1 alpha/HIF-2 alpha, leading to steric clash that prevents the recruitment of the co-activators p300 and CBP. On the other hand, under hypoxic conditions, FIH1 is inactive, which results in activation of HIF-alpha signaling. FIH1 is involved in transcriptional repression through interaction with HIF1A, VHL and HDAC and hydroxylates specific Asn residues within ARD (ankyrin repeat domains) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A as well as other ARD-containing proteins. FIH1 also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. FIH1 has the ability to negatively regulate NOTCH1 activity thus accelerating myogenic differentiation, and positively regulates ASB4 activity which promotes vascular differentiation. FIH1-Mint3 binding affects FIH1's ability to modify HIF-1 alpha in an oxygen-independent manner and FIH1-Bax interaction plays inhibitory role in apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for FIH-1/HIF-1AN Antibody (NB100-428)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: EM, ICC/IF, IHC-P, IP, WB.


Filter By Application
EM
(1)
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(9)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-428 Applications Species
Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016 May 31 [PMID: 27245613] (WB, Human) WB Human
Nakaoka HJ, Hara T, Yoshino S et al. NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells Sci Rep Mar 7 2016 [PMID: 26948053] (IP, Human) IP Human
Kiriakidis S, Henze AT, Kruszynska-Ziaja I et al. Factor-inhibiting HIF-1 (FIH-1) is required for human vascular endothelial cell survival FASEB J. 2015 Apr 02 [PMID: 25837583] (WB, Human) WB Human
Karttunen S, Duffield M, Scrimgeour Nr et al. Oxygen-dependent hydroxylation by Factor Inhibiting HIF (FIH) regulates the TRPV3 ion channel. J. Cell. Sci. 2014 Nov 20 [PMID: 25413349] (WB) WB
Rawluszko AA, Bujnicka KE, Horbacka K et al. Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer. BMC Cancer. 2013 Nov 6 [PMID: 24195777] (WB, Human) WB Human
Janke K, Brockmeier U, Kuhlmann K et al. Factor Inhibiting HIF-1 (FIH-1) modulates protein interactions of Apoptosis-Stimulating p53 binding Protein 2 (ASPP2). J Cell Sci 2013 Apr 19 [PMID: 23606740] (WB, Human) WB Human
Nishida C, Kusubata K, Tashiro Y et al. MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells Blood 2012 Jun 7 [PMID: 22544701]
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Increased activation of the hypoxia-inducible factor pathway in varicose veins. J Vasc Surg;55(5):1427-1439e1. 2012 May. [PMID: 22277691] (WB, Human) WB Human
Wilkins SE, Karttunen S, Hampton-Smith RJ et al. Factor inhibiting HIF (FIH) recognises distinct molecular features within hypoxia inducible factor (HIF)-[alpha] versus ankyrin repeat substrates. The Journal of Biological Chemistry. 2012 Jan 23. [PMID: 22270367]
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell 2011 Nov [PMID: 21900490] (Human) Human
Show All 20 Publications.

Reviews for FIH-1/HIF-1AN Antibody (NB100-428) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-428:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot FIH-1/HIF-1AN NB100-428
Enlarge
reviewed by:
Anonymous
WB Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedCell lysates from prostate cancer cell lines
SpeciesHuman
LotA2

Comments

CommentsSample: Whole cell lysates of prostate cancer cell lines (20ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22277691
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22277691

Details

Detection NotesSee PMID 22277691

Comments

CommentsPublished in PMID 22277691

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NB100-428) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-428AF405
Alexa Fluor 488 Labeled NB100-428AF488
Alexa Fluor 647 Labeled NB100-428AF647
Alexa Fluor 700 Labeled NB100-428AF700
Biotin Labeled NB100-428B
DyLight 405 Labeled NB100-428V
DyLight 488 Labeled NB100-428G
DyLight 550 Labeled NB100-428R
DyLight 650 Labeled NB100-428C
FITC Labeled NB100-428F
HRP Labeled NB100-428H

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
11/12/2017
Application: WB
Species: Human

 
Anonymous
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIF1AN
Entrez
Uniprot