EGLN3/PHD3 Antibody


Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Analysis of HeLa lysates using NB100-139. Image courtesy of Dr Gregg Semenza (The Johns Hopkins University School of Medicine, Baltimore, MD USA)
Immunocytochemistry/ Immunofluorescence: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight more
Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB more
Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. Primary antibody PHD3 NB100-139 was used at a dilution of more
Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P, IP
1 mg/ml

EGLN3/PHD3 Antibody Summary

Synthetic peptide corresponding to residues between 50-100 of human PHD3/HIF Prolyl Hydroxylase 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
In WB, PHD3 band can be seen at 27-30 kDa molecular weight range. Use of this antibody in Electron Microscopy and ChIP is reported in scientific literature - PMIDs 17003483and 24367580 respectively. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
28 kDa
Reviewed Applications
Read 2 Reviews rated 5
NB100-139 in the following application:

Read Publications using
NB100-139 in the following applications:

  • EM
    1 publication
  • 3 publications
  • 2 publications
  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24037093)

Alternate Names for EGLN3/PHD3 Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3


HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitination complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, TCS, LA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, EM, ICC/IF, IHC, IHC-P, IP, ICC
Species: Hu
Applications: WB, IP
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICFlow
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP

Publications for EGLN3/PHD3 Antibody (NB100-139)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: EM, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB100-139 Applications Species
Adam MG, Matt S, Christian S et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle. 2015 Dec 02 [PMID:26654769] (WB, Human) WB Human
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 2015 Jul 30 [PMID:26223520] (WB, ICC/IF, Human) WB, ICC/IF Human
Li J, Yuan W, Jiang S et al. Prolyl-4-hydroxylase Domain Protein 2 Controls NF-kB/p65 Transactivation and Enhances the Catabolic Effects of Inflammatory Cytokines on cells of the Nucleus Pulposus J. Biol. Chem. 2015 Jan 29 [PMID:25635047] (WB, Rat, Human) WB Rat, Human
Vogler M, Zieseniss A, Hesse AR et al. Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult Pflugers Arch. 2015 Jan 13 [PMID:25578858] (IHC-P, Mouse) IHC-P Mouse
Place TL, Nauseef JT, Peterson MK et al. Prolyl-4-Hydroxylase 3 (PHD3) Expression Is Downregulated during Epithelial-to-Mesenchymal Transition. PLoS One 2013 Dec 18 [PMID:24367580] (WB, IP, Human) WB, IP Human
Rawluszko AA, Bujnicka KE, Horbacka K et al. Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer. BMC Cancer. 2013 Nov 6 [PMID:24195777] (WB, Human) WB Human
Taniguchi CM, Finger EC, Krieg AJ et al. Cross-talk between hypoxia and insulin signaling through Phd3 regulates hepatic glucose and lipid metabolism and ameliorates diabetes. Nat Med. 2013 Oct [PMID:24037093] (WB, Mouse) WB Mouse
Galan-Cobo A, Sanchez-Silva R, Serna A et al. Cellular overexpression of aquaporins slows down the natural HIF-2a degradation during prolonged hypoxia. Gene. 2013 Jul 13 [PMID:23545307] (WB, Rat) WB Rat
Fujita N, Markova D, Anderson DG, Chiba K, Toyama Y, Shapiro IM, Risbud MV. Expression of Prolyl Hydroxylases (PHDs) Is Selectively Controlled by HIF-1 and HIF-2 Proteins in Nucleus Pulposus Cells of the Intervertebral Disc: DISTINCT ROLES OF PHD2 AND PHD3 PROTEINS IN CONTROLLING HIF-1? ACTIVITY IN HYPOXIA. J Biol Chem;287(20):16975-86. 2012 May 11. [PMID:22451659] (ICC/IF, WB, Human, Rat) ICC/IF, WB Human, Rat
Hogel H, Rantanen K, Jokilehto T et al. Prolyl hydroxylase PHD3 enhances the hypoxic survival and G1 to S transition of carcinoma cells. PLoS One. 2011 [PMID:22087251] (WB, Human) WB Human
Show All 31 Publications.

Reviews for EGLN3/PHD3 Antibody (NB100-139) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1 application: WB.

Reviews using NB100-139:
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Images Ratings Applications Species Date Details
Western Blot EGLN3/PHD3 NB100-139
reviewed by:
WB Mouse 05/08/2016


ApplicationWestern Blot
FileView PDF
reviewed by:
WB Human 06/24/2010


ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug


Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP


Detection NotesDetection Method: ECL Plus, Specific Bands: 27 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGLN3/PHD3 Antibody (NB100-139) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-139AF405
Alexa Fluor (R) 488 Labeled NB100-139AF488
Alexa Fluor (R) 647 Labeled NB100-139AF647
Alexa Fluor (R) 700 Labeled NB100-139AF700
Biotin Labeled NB100-139B
DyLight 405 Labeled NB100-139V
DyLight 488 Labeled NB100-139G
DyLight 550 Labeled NB100-139R
DyLight 650 Labeled NB100-139C
FITC Labeled NB100-139F
HRP Labeled NB100-139H

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Contact Information

Product PDFs


Gene Symbol EGLN3

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