EGLN3/PHD3 Antibody


Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Analysis of HeLa lysates using NB100-139. Image courtesy of Dr Gregg Semenza (The Johns Hopkins University School of Medicine, Baltimore, MD USA)
Immunocytochemistry/ Immunofluorescence: EGLN3/PHD3 Antibody [NB100-139] - EGLN3/PHD3 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight more
Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB more
Western Blot: EGLN3/PHD3 Antibody [NB100-139] - Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. Primary antibody PHD3 NB100-139 was used at a dilution of more
Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [NB100-139] - Analysis of a formalin fixed tissue section of human renal cell carcinoma (clear cell type) using rabbit polyclonal EGLN3/PHD3 antibody with HRP-DAB more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P, IP
1 mg/ml

EGLN3/PHD3 Antibody Summary

Synthetic peptide corresponding to residues between 50-100 of human PHD3/HIF Prolyl Hydroxylase 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000-1:2000
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:250-1:1000
  • Immunohistochemistry-Paraffin 1:250-1:1000
  • Immunoprecipitation
Application Notes
In WB, PHD3 band can be seen at 27-30 kDa molecular weight range. Use of this antibody in Electron Microscopy and ChIP is reported in scientific literature - PMIDs 17003483and 24367580 respectively. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
EGLN3/PHD3 Lysate (NBL1-10156)
Reviewed Applications
Read 2 Reviews rated 5
NB100-139 in the following applications:

Read Publications using
NB100-139 in the following applications:

  • EM
    1 publication
  • 3 publications
  • 2 publications
  • IP
    1 publication
  • WB
    16 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24037093)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for EGLN3/PHD3 Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3


HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitination complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
Species: Hu
Applications: WB, IP
Species: Hu
Applications: WB, IP
Species: Hu
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
Applications: WB, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: CyTOF-ready, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP

Publications for EGLN3/PHD3 Antibody (NB100-139)(37)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: EM, ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB100-139 Applications Species
Bryant JD, Brown MC, Dobrikov MI et al. Regulation of HIF-1A during Hypoxia by DAP5-Induced Translation of PHD2. Mol. Cell. Biol. Mar 12 2018 [PMID: 29530922] (WB) WB
Miikkulainen P, Hogel H, Rantanen K et al. HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma Cancer Metab 2017 Jul 06 [PMID: 28680592] (WB, Human) WB Human
Bruno T, Valerio M, Casadei L et al. Che-1 sustains hypoxic response of colorectal cancer cells by affecting Hif-1alpha stabilization. J. Exp. Clin. Cancer Res. 2017 Feb 18 [PMID: 28214471] (WB, Human) WB Human
Oh ET, Kim JW, Kim JM et al. NQO1 inhibits proteasome-mediated degradation of HIF-1a. Nat Commun. Dec 14 2016 [PMID: 27966538] (Mouse) Mouse
Barnes EA, Chen CH, Sedan O, Cornfield DN. Loss of smooth muscle cell hypoxia inducible factor-1a underlies increased vascular contractility in pulmonary hypertension FASEB J. 2017 Feb 01 [PMID: 27811062] (WB, Human) WB Human
Adam MG, Matt S, Christian S et al. SIAH ubiquitin ligases regulate breast cancer cell migration and invasion independent of the oxygen status. Cell Cycle. 2015 Dec 02 [PMID: 26654769] (WB, Human) WB Human
Iriondo O, Rabano M, Domenici G et al. Distinct breast cancer stem/progenitor cell populations require either HIF1alpha or loss of PHD3 to expand under hypoxic conditions. Oncotarget 2015 Oct 13 [PMID: 26372732] (WB) WB
Hogel H, Miikkulainen P, Bino L, Jaakkola PM. Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27. Mol. Cancer. 2015 Jul 30 [PMID: 26223520] (WB, ICC/IF, Human)

PHD3 antibody used for WB and ICC/IF on human samples. ICC/IF performed on siPHD3-depleted HeLa cells (Figure 2A). WB carried out on HeLa and 786-O cells that were transfected with PHD3 siRNA followed by synchronization at G0 and 24-h hypoxic exposure (Figure 1).
WB, ICC/IF Human
Li J, Yuan W, Jiang S et al. Prolyl-4-hydroxylase Domain Protein 2 Controls NF-kB/p65 Transactivation and Enhances the Catabolic Effects of Inflammatory Cytokines on cells of the Nucleus Pulposus J. Biol. Chem. 2015 Jan 29 [PMID: 25635047] (WB, Rat, Human)

EGLN3/PHD3 antibody used for WB on lysates from nucleus pulposus/NP cells (rat) that were transduced with Sh-control or Sh-PHD2 with or without TNF-alpha treatment (FIGURE 1-I). Antibody also used for WB analysis of immunoprecipitates obtained from HEK293T cells that were transfected with plasmids of FLAG-p65, YFP-PHD2 or PHD3 (FIGURE 6B)
WB Rat, Human
Vogler M, Zieseniss A, Hesse AR et al. Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult Pflugers Arch. 2015 Jan 13 [PMID: 25578858] (IHC-P, Mouse)

PHD3/HIF Prolyl Hydroxylase 3 antibody used at 1:10,000 dilution for IHC-P application on heart sections from C57BL/6 mice after 1, 6, and 24 h of treatment (40-fold magnification) with ICA, a specific PHD inhibitor 2-(1-chloro-4-hydroxyisoquinoline-3-carboxamido) acetate - two doses of 40 mg/kg BW ICA or vehicle i.p., either 6 and 1 h before myocardial infarction or 1 and 5 h after myocardial infarction (Fig 3).
IHC-P Mouse
Show All 37 Publications.

Reviews for EGLN3/PHD3 Antibody (NB100-139) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-139:
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Images Ratings Applications Species Date Details
Western Blot EGLN3/PHD3 NB100-139
reviewed by:
WB Mouse 05/08/2016


ApplicationWestern Blot
FileView PDF
reviewed by:
WB Human 06/24/2010


ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 20 ug


Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit IgG-HRP


Detection NotesDetection Method: ECL Plus, Specific Bands: 27 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EGLN3/PHD3 Antibody (NB100-139) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-139AF405
Alexa Fluor 488 Labeled NB100-139AF488
Alexa Fluor 647 Labeled NB100-139AF647
Alexa Fluor 700 Labeled NB100-139AF700
Biotin Labeled NB100-139B
DyLight 405 Labeled NB100-139V
DyLight 488 Labeled NB100-139G
DyLight 550 Labeled NB100-139R
DyLight 650 Labeled NB100-139C
FITC Labeled NB100-139F
HRP Labeled NB100-139H

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Recent Reviews


Application: WB
Species: Mouse

Application: WB
Species: Human


Gene Symbol EGLN3