PAG1 Antibody (PAG-C1) - BSA Free

Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.

Immunoprecipitation: PAG1 Antibody (PAG-C1) [NB500-487] - Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255). Note: Csk-binding protein (Cbp/PAG) is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-Cbp/PAG (PAG-C1) Lane 3: immunoprecipitation with anti-Cbp/PAG (polyclonal antibody).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC, IP
• Clone: PAG-C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PAG1 Antibody (PAG-C1) - BSA Free Summary

• Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
• Epitope: Located in the C-terminal domain.
• Specificity: The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:100-1:2000
• Application Notes: Immunohistochemistry-Paraffin sections: heat-mediated antigen retrieval in citrate buffer pH 6.1
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for PAG1 Antibody (PAG-C1) - BSA Free

• CBP
• CBPMGC138364
• Csk-binding protein
• PAG1
• PAGFLJ37858
• phosphoprotein associated with glycosphingolipid microdomains 1
• phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• Transmembrane adapter protein PAG
• Transmembrane phosphoprotein Cbp

Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAG1 Antibody (NB500-487)(1)

Publication using NB500-487

• Yamashita M, Chattopadhyay S, Fensterl V et al. A TRIF-Independent Branch of TLR3 Signaling J Immunol 2012-03-15 [PMID: 22323545] (WB, Mouse)

Bioinformatics

• Gene Symbol: PAG1
• Entrez:
  • 55824(Human)
  • 94212(Mouse)
  • 64019(Rat)