PAG1 Antibody (PAG-C1) - BSA Free

Images

 
Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody ...read more
Immunoprecipitation: PAG1 Antibody (PAG-C1) [NB500-487] - Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IP
Clone
PAG-C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

PAG1 Antibody (PAG-C1) - BSA Free Summary

Description
Novus Biologicals Mouse PAG1 Antibody (PAG-C1) - BSA Free (NB500-487) is a monoclonal antibody validated for use in IHC, WB and IP. Anti-PAG1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Epitope
Located in the C-terminal domain.
Specificity
The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PAG1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:100-1:2000
Application Notes
Immunohistochemistry-Paraffin sections: heat-mediated antigen retrieval in citrate buffer pH 6.1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB500-487 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate buffered saline (PBS), pH 7.4
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for PAG1 Antibody (PAG-C1) - BSA Free

  • CBP
  • CBPMGC138364
  • Csk-binding protein
  • PAG1
  • PAGFLJ37858
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein associated with glycosphingolipid-enriched microdomains 1
  • Transmembrane adapter protein PAG
  • Transmembrane phosphoprotein Cbp

Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAG1 Antibody (NB500-487)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

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Bioinformatics

Gene Symbol PAG1
Entrez