CBP/KAT3A Antibody

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Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) and Texs ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human RelA (NBP2-22297) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human CBP (NB100-381). Image merged from DAPI ...read more
Proximity Ligation Assay: CBP/KAT3A Antibody [NB100-381] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human CBP (NB100-381). Image merged from DAPI (2ms) and Texs ...read more
Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CBP/KAT3A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 975 and 1025 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CREBBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
This antibody immunoprecipitates CBP. It also recognizes a band at ~240 kDa in Western blot, representing CBP. It is strongly recommended that the investigator use nuclear extracts for optimal results. ChIP - See customer review
Theoretical MW
265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-381 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-1567, NB200-171, NB100-97831, NB100-58826

Alternate Names for CBP/KAT3A Antibody

  • CBP
  • CBPRTS
  • CREB binding protein
  • CREB-binding protein
  • CREBBP
  • EC 2.3.1.48
  • KAT3A
  • RSTS
  • RTS
  • Rubinstein-Taybi syndrome

Background

CREB-binding protein (CBP) and p300 contain modular domains that mediate protein-protein interactions with a wide variety of nuclear factors. C-terminal domain is responsible for interaction with specific coactivators and other transcriptional regulators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CBP/KAT3A Antibody (NB100-381) (0)

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Reviews for CBP/KAT3A Antibody (NB100-381) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-381:
Filter by Applications
ChIP
(1)
IP
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation CBP/KAT3A NB100-381
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reviewed by:
Anonymous
ChIP Human 11/16/2010
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Summary

ApplicationChromatin Immunoprecipitation
Sample Testedhypoxic hela cell
SpeciesHuman

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 3 ug, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Method: Real-time PCR, ChIP Type: Cross-linking (X-ChIP)
Immunoprecipitation CBP/KAT3A NB100-381
Enlarge
reviewed by:
Anonymous
IP Human 11/17/2009
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa cell lysates
SpeciesHuman
LotA1
CommentsLysate Amount: 2mg, Ab Dilution Ratio: 3ug/ml, Protein Capture: Protein A

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Comments

CommentsLysate Amount: 2mg, Ab Dilution Ratio: 3ug/ml, Protein Capture: Protein A

Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for CBP/KAT3A Antibody (NB100-381) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
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1
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Anonymous
11/16/2010
Application: ChIP
Species: Human

Anonymous
11/17/2009
Application: IP
Species: Human

Bioinformatics

Gene Symbol CREBBP
Entrez
Uniprot