MCG10 Antibody - BSA Free

Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.

Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - HeLa cells with MCG10 antibody at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - Hela cells.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MCG10 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10. The immunogen is located within the last 50 amino acids of MCG10. Amino Acid Squence: AAANGSKKAERQKFSPY
• Predicted Species: Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCBP4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1-20 ug/ml
  • Immunoprecipitation
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID: 27302059).
• Control Peptide:

  MCG10 Peptide (NBP1-76729PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MCG10 Antibody - BSA Free

• alphaCP-4 protein
• alpha-CP4
• CBP
• LIP4
• LYST-interacting protein
• MCG10
• poly(rC) binding protein 4
• poly(rC)-binding protein 4
• RNA binding protein MCG10

Background

MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCG10 Antibody (NBP1-76729)(2)

Publications using NBP1-76729

• Yanatori I, Richardson DR, Toyokuni S, Kishi F. The Iron Chaperone Poly (rC)-binding Protein 2 Forms a Metabolon with the Heme Oxygenase 1/Cytochrome P450 Reductase Complex for Heme Catabolism and Iron Transfer J. Biol. Chem. 2017-06-27 [PMID: 28655775] (Human)
• Yanatori I, Richardson DR, Imada K, Kishi F. Iron Export through the Transporter Ferroportin 1 Is Modulated by the Iron Chaperone PCBP2. J Biol Chem 2016-08-12 [PMID: 27302059] (IP)

Bioinformatics

• Gene Symbol: PCBP4
• Uniprot:
  • EAW65166(Human)