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MCG10 Antibody

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Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.
Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.
Immunocytochemistry/ Immunofluorescence: MCG10 Antibody [NBP1-76729] - HeLa cells with MCG10 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: MCG10 Antibody [NBP1-76729] - Hela cells.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MCG10 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10. The immunogen is located within the last 50 amino acids of MCG10. Amino Acid Squence: AAANGSKKAERQKFSPY
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCBP4
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/mL
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1-20 ug/ml
  • Immunoprecipitation
Application Notes
This antibody can be used for detection of MCG10 by Western blot at 0.5 - 1 mg/ml. Use in immunoprecipitation reported in scientific literature (PMID: 27302059).
Control Peptide
MCG10 Peptide (NBP1-76729PEP)
Publications
Read Publications using
NBP1-76729 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Peptide affinity purified

Alternate Names for MCG10 Antibody

  • alphaCP-4 protein
  • alpha-CP4
  • CBP
  • LIP4
  • LYST-interacting protein
  • MCG10
  • poly(rC) binding protein 4
  • poly(rC)-binding protein 4
  • RNA binding protein MCG10

Background

MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the PE format of this antibody.
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Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-382. For blotting immunoprecipitated CBP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.
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MCG10 Antibody
NBP1-76729
MCG10 Antibody
Species: Hu, Mu, Bv
Applications: WB, ELISA, ICC/IF, IP

Publications for MCG10 Antibody (NBP1-76729)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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Bioinformatics

Gene Symbol PCBP4
Uniprot