MCG10 Antibody

Images

 
Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.
Immunocytochemistry/ Immunofluorescence: MCG10 Antibody [NBP1-76729] - HeLa cells with MCG10 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: MCG10 Antibody [NBP1-76729] - Hela cells.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MCG10 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10. The immunogen is located within the last 50 amino acids of MCG10.
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCBP4
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation
Application Notes
This antibody can be used for detection of MCG10 by Western blot at 0.5 - 1 mg/ml. Use in immunoprecipitation reported in scientific literature (PMID: 27302059).
Control Peptide
Publications
Read Publications using
NBP1-76729 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Peptide affinity purified

Alternate Names for MCG10 Antibody

  • alphaCP-4 protein
  • alpha-CP4
  • CBP
  • LIP4
  • LYST-interacting protein
  • MCG10
  • poly(rC) binding protein 4
  • poly(rC)-binding protein 4
  • RNA binding protein MCG10

Background

MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCG10 Antibody (NBP1-76729)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


Filter By Application
IP
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MCG10 Antibody (NBP1-76729) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCBP4
Uniprot