MMP-14/MT1-MMP Antibody (3-F7)

Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Human endometrial adenocarcinoma paraffin-embedded tissue section. Tissue section was fixed, deparaffinized and EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody. IHC-P image submitted by a verified customer review.

Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: 3-F7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MMP-14/MT1-MMP Antibody (3-F7) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MMP-14/MT1-MMP aa 149-200 / 582. (SwissProt: P50281 Human; SwissProt: P53690 Mouse; SwissProt: Q10739 Rat)
• Localization: Membrane, Melanosome, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MMP14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MMP-14/MT1-MMP Antibody (3-F7)

• EC 3.4.24
• EC 3.4.24.80
• matrix metallopeptidase 14 (membrane-inserted)
• matrix metalloproteinase 14 (membrane-inserted)
• matrix metalloproteinase-14
• membrane type 1 metalloprotease
• Membrane-type matrix metalloproteinase 1
• Membrane-type-1 matrix metalloproteinase
• MMP14
• MMP-14
• MMP-X1
• MT1MMP
• MT1-MMP
• MT1-MMPMTMMP1
• MT-MMP 1
• MT-MMP1

Background

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-14/MT1-MMP Antibody (NBP2-67415)(2)

Publications using NBP2-67415

• Danilo Pellin, Natalie Claudio, Zihan Guo, Tahereh Ziglari, Ferdinando Pucci Gene Expression Profiling of Lymph Node Sub-Capsular Sinus Macrophages in Cancer Frontiers in Immunology 2021-06-08 [PMID: 34168645]
• Feng Y, Cai H, Huang X et al. Active MT1-MMP is tethered to collagen fibers in DDR2-containing remnants Gene 2021-04-18 [PMID: 33882324]

Reviews for MMP-14/MT1-MMP Antibody (NBP2-67415) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Shinford Sun WB Human 09/16/2024

Summary

• Application: Western Blot
• Sample Tested: Endothelial
• Species: Human
• Lot: H661919013

reviewed by: Verified Customer IHC-P Human 10/17/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Endometrial adenocarcinoma
• Species: Human
• Lot: 3-F7

Comments

• Comments: Fixed paraffin-embedded sections. Tissue sections were deparaffinized. EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody.

Bioinformatics

• Gene Symbol: MMP14