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MMP-14/MT1-MMP Antibody (3-F7)

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Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Human endometrial adenocarcinoma paraffin-embedded tissue section. Tissue section was fixed, deparaffinized and EDTA was used for antigen ...read more
Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). ...read more
Immunocytochemistry/ Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
3-F7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MMP-14/MT1-MMP Antibody (3-F7) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human MMP-14/MT1-MMP aa 149-200 / 582. (SwissProt: P50281 Human; SwissProt: P53690 Mouse; SwissProt: Q10739 Rat)
Localization
Membrane, Melanosome, Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MMP14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-67415 in the following applications:

Publications
Read Publication using NBP2-67415.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MMP-14/MT1-MMP Antibody (3-F7)

  • EC 3.4.24
  • EC 3.4.24.80
  • matrix metallopeptidase 14 (membrane-inserted)
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metalloproteinase-14
  • membrane type 1 metalloprotease
  • Membrane-type matrix metalloproteinase 1
  • Membrane-type-1 matrix metalloproteinase
  • MMP14
  • MMP-14
  • MMP-X1
  • MT1MMP
  • MT1-MMP
  • MT1-MMPMTMMP1
  • MT-MMP 1
  • MT-MMP1

Background

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-14/MT1-MMP Antibody (NBP2-67415)(1)


Review for MMP-14/MT1-MMP Antibody (NBP2-67415) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-67415:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMP-14/MT1-MMP NBP2-67415
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5
reviewed by:
Verified Customer
IHC-P Human 10/17/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedEndometrial adenocarcinoma
SpeciesHuman
Lot3-F7

Comments

CommentsFixed paraffin-embedded sections. Tissue sections were deparaffinized. EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-14/MT1-MMP Antibody (NBP2-67415) (0)

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Secondary Antibodies

 

Isotype Controls

Additional MMP-14/MT1-MMP Products

Research Areas for MMP-14/MT1-MMP Antibody (NBP2-67415)

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Blogs on MMP-14/MT1-MMP

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Recent Reviews

5
5
1
4
0
3
0
2
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1
0

Verified Customer
10/17/2021
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MMP14