FIH-1/HIF-1AN Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

FIH-1/HIF-1AN Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIF1AN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Factor Inhibiting HIF-1 antibody is useful for Immunohistochemistry, Immunoprecipitation and Western Blot. In WB, a band at ~40.3 kDa is seen. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, rat and mouse (PMID 19720742).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FIH-1/HIF-1AN Antibody [DyLight 650]

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIH-1/HIF-1AN Antibody (NB100-428C) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-428B
DyLight 488 Labeled NB100-428G
DyLight 550 Labeled NB100-428R
HRP Labeled NB100-428H

Additional FIH-1/HIF-1AN Products

Bioinformatics Tool for FIH-1/HIF-1AN Antibody (NB100-428C)

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Diseases for FIH-1/HIF-1AN Antibody (NB100-428C)

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Research Areas for FIH-1/HIF-1AN Antibody (NB100-428C)

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Bioinformatics

Gene Symbol HIF1AN
Entrez