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CXCR4 Antibody

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Western Blot: CXCR4 Antibody [NBP1-77560] - Analysis of CXCR4 in (A) HeLa and (B) A431 cell lysate.
Western Blot: CXCR4 Antibody [NBP1-77560] - Analysis of CXCR4 in (A) HeLa and (B) A431 cell lysate.
Simple Western: CXCR4 Antibody [NBP1-77560] - Lane view shows a specific band for CXCR4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77560AF350
Alexa Fluor 405
NBP1-77560AF405
Alexa Fluor 488
NBP1-77560AF488
Alexa Fluor 532
NBP1-77560AF532
Alexa Fluor 594
NBP1-77560AF594
Alexa Fluor 647
NBP1-77560AF647
Alexa Fluor 700
NBP1-77560AF700
Alexa Fluor 750
NBP1-77560AF750
Biotin
NBP1-77560B
DyLight 350
NBP1-77560UV
DyLight 405
NBP1-77560V
DyLight 488
NBP1-77560G
DyLight 550
NBP1-77560R
DyLight 594
NBP1-77560DL594
DyLight 650
NBP1-77560C
DyLight 680
NBP1-77560FR
DyLight 755
NBP1-77560IR
HRP
NBP1-77560H
Janelia Fluor 549
NBP1-77560JF549
Janelia Fluor 646
NBP1-77560JF646
100% Guarantee on every product

CXCR4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human CXCR4 protein (between residues 300-352) [UniProt# P61073]
Localization
Cell membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXCR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:25
Application Notes
This CXCR4 antibody is useful for Western blot, where a band is seen ~45 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Whole Cell Lysate
CXCR4 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP1-77560 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CXCR4 Antibody

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediate hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha  was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
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Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
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Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
(19 Publications)
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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NBP2-22203
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Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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CXCR3 Antibody
(49801) [Unconjugated
MAB160
Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A).View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND50 is typically 0.3-1.5 µg/mL.
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CCR1 Antibody
(53504) [Unconjugated
MAB145
CCR1 Antibody (53504) [Unconjugated]
(1 Review)
(22 Publications)
Species: Hu
Applications: Flow, CyTOF-ready

Publications for CXCR4 Antibody (NBP1-77560)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for CXCR4 Antibody (NBP1-77560): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CXCR4 Antibody (NBP1-77560). (Showing 1 - 1 of 1 FAQ).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Control Lysate(s)

HeLa Whole Cell Lysate
CXCR4 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77560AF350
Alexa Fluor 405 NBP1-77560AF405
Alexa Fluor 488 NBP1-77560AF488
Alexa Fluor 532 NBP1-77560AF532
Alexa Fluor 594 NBP1-77560AF594
Alexa Fluor 647 NBP1-77560AF647
Alexa Fluor 700 NBP1-77560AF700
Alexa Fluor 750 NBP1-77560AF750
Biotin NBP1-77560B
DyLight 350 NBP1-77560UV
DyLight 405 NBP1-77560V
DyLight 488 NBP1-77560G
DyLight 550 NBP1-77560R
DyLight 594 NBP1-77560DL594
DyLight 650 NBP1-77560C
DyLight 680 NBP1-77560FR
DyLight 755 NBP1-77560IR
HRP NBP1-77560H
Janelia Fluor 549 NBP1-77560JF549
Janelia Fluor 646 NBP1-77560JF646
Learn about Custom Antibody Labeling

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Blogs on CXCR4.

Check out the latest blog posts on CXCR4.
Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.
LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.
CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.
Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.
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Bioinformatics

Gene Symbol CXCR4
Entrez
Uniprot