CXCR4 Antibody - BSA Free

Western Blot: CXCR4 Antibody [NBP1-77560] - Analysis of CXCR4 in (A) HeLa and (B) A431 cell lysate.

Simple Western: CXCR4 Antibody [NBP1-77560] - Lane view shows a specific band for CXCR4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CXCR4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CXCR4 Antibody - BSA Free (NBP1-77560) is a polyclonal antibody validated for use in WB and Simple Western. Anti-CXCR4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal portion of the human CXCR4 protein (between residues 300-352) [UniProt# P61073]
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:25
  • Western Blot 2 ug/ml
• Application Notes: This CXCR4 antibody is useful for Western blot, where a band is seen ~45 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 51 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCR4 Antibody - BSA Free

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediate hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (NBP1-77560)(2)

Publications using NBP1-77560

• Zhang F, Zheng L, Wu Y et al. E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses. Cellular and Molecular Life Sciences: CMLS 2025-06-24 [PMID: 40549002]
• Farhan MA, Azad AK, Touret N, Murray AG. FGD5 (Faciogenital Dysplasia-5) Regulates VEGF (Vascular Endothelial Growth Factor-A) Receptor-2 Coupling to PI3 (Phosphoinositide-3) Kinase and Receptor Recycling. Arterioscler Thromb Vasc Biol. 2017-10-19 [PMID: 29051140] (WB, Human)
  
  Details:
  This citation used the HRP form of this antibody.

FAQs for CXCR4 Antibody (NBP1-77560). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 7852(Human)
• Uniprot:
  • P61073()