CCR1 Antibody (53504) [Unconjugated]

PBMC Monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB145) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 53504
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR1 Antibody (53504) [Unconjugated] Summary

• Additional Information: Clone 53504 was used by HLDA to establish CD designation
• Immunogen: Swiss 3T3 mouse cell line transfected with human CCR1
  Accession # P32246
• Specificity: Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry.
  No cross‑reactivity with CCR5 transfectants is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody (53504) [Unconjugated]

• C-C chemokine receptor type 1
• CC-CKR-1
• CCR1
• CCR-1
• CD191 antigen
• CD191
• chemokine (C-C motif) receptor 1
• CKR1
• CKR-1
• CMKBR1SCYAR1
• CMKR1
• HM145C-C CKR-1
• LD78 receptor
• Macrophage inflammatory protein 1-alpha receptor
• MIP-1alpha-R
• MIP1aR
• RANTES receptor
• RANTES-R

Background

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR1 Antibody (MAB145)(26)

Publications using MAB145

• GA Enten, X Gao, HR Strzelinsk, M Weche, SB Liggett, M Majetschak alpha1B/D-adrenoceptors regulate chemokine receptor-mediated leukocyte migration via formation of heteromeric receptor complexes Proceedings of the National Academy of Sciences of the United States of America, 2022-05-10;119(20):e2123511119. 5/10/2022 [PMID: 35537053]
• Y Tian, G Seumois, LM De-Oliveir, J Mateus, S Herrera-de, C Kim, D Hinz, NDS Goonawardh, AD de Silva, S Premawansa, G Premawansa, A Wijewickra, A Balmaseda, A Grifoni, P Vijayanand, E Harris, B Peters, A Sette, D Weiskopf Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4�T Cells and Their Association with Dengue Disease Cell Rep, 2019-12-24;29(13):4482-4495.e4. 2019-12-24 [PMID: 31875555] (Flow Cytometry, Human)
• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 2019-04-30 [PMID: 31040288]
• JM Fox, F Kausar, A Day, M Osborne, K Hussain, A Mueller, J Lin, T Tsuchiya, S Kanegasaki, JE Pease CXCL4/Platelet Factor 4 is an agonist of CCR1 and drives human monocyte migration Sci Rep, 2018-06-21;8(1):9466. 2018-06-21 [PMID: 29930254] (Flow Cytometry, Mouse)
• K Vandyke, MN Zeissig, DR Hewett, SK Martin, KM Mrozik, CM Cheong, P Diamond, LB To, S Gronthos, DJ Peet, PI Croucher, ACW Zannettino HIF-2? promotes dissemination of plasma cells in multiple myeloma by regulating CXCL12/CXCR4 and CCR1 Cancer Res., 2017-08-30;0(0):. 2017-08-30 [PMID: 28855206] (Flow Cytometry, Human)
• D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017-08-17;7(1):8536. 2017-08-17 [PMID: 28819198] (ICC, Human)
• F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages J. Immunol., 2017-07-12;0(0):. 2017-07-12 [PMID: 28701512] (Flow Cytometry, Human)
• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016-08-05;0(0):. 2016-08-05 [PMID: 27496101] (Flow Cytometry, Human)
• Gilliland C, Salanga C, Kawamura T, Trejo J, Handel T The chemokine receptor CCR1 is constitutively active, which leads to G protein-independent, beta-arrestin-mediated internalization. J Biol Chem, 2013-09-20;288(45):32194-210. 2013-09-20 [PMID: 24056371] (Flow Cytometry, Human)
• Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors Experimental and Molecular Medicine 2011-10-31 [PMID: 21847008]
• Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur. J. Immunol., 2011-03-07;41(4):963-73. 2011-03-07 [PMID: 21381021] (Neutralization, Human)
• Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010-07-15;127(2):332-44. 2010-07-15 [PMID: 19937793] (Neutralization, Human)
• Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells. Int. J. Cancer, 2010-03-01;126(5):1095-108. 2010-03-01 [PMID: 19642141] (Neutralization, Human)
• Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009-07-10;21(8):991-1001. 2009-07-10 [PMID: 19592420] (Flow Cytometry, Western Blot, Mouse)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, ICC, IHC-P, Neutralization, Human)
• Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation. Am. J. Transplant., 2008-07-01;8(7):1512-22. 2008-07-01 [PMID: 18513272] (IHC-P, Human)
• Joubert P, Lajoie-Kadoch S, Welman M, Dragon S, Letuvee S, Tolloczko B, Halayko AJ, Gounni AS, Maghni K, Hamid Q Expression and regulation of CCR1 by airway smooth muscle cells in asthma. J. Immunol., 2008-01-15;180(2):1268-75. 2008-01-15 [PMID: 18178867] (IHC-P, Human)
• Gupta S, Fuchs B, Schulz-Maronde S, Heitland A, Escher SE, Mack M, Tillmann HC, Braun A, Forssmann WG, Elsner J, Forssmann U Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation. J. Leukoc. Biol., 2007-12-06;83(3):765-73. 2007-12-06 [PMID: 18063698] (Flow Cytometry, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (Flow Cytometry, Human)
• Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-03-29;25(7):1737-45. 2007-03-29 [PMID: 17395768] (Flow Cytometry, Human)
• Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2005-08-17;65(3):294-300. 2005-08-17 [PMID: 16107514] (IHC-Fr, Human)
• Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Eur. J. Immunol., 2004-12-01;34(12):3485-96. 2004-12-01 [PMID: 15484187] (Flow Cytometry, Human)
• Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003-12-04;103(7):2806-8. 2003-12-04 [PMID: 14656893] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Neutralization, Human)
• Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol, 2001-06-01;166(11):6477-82. 2001-06-01 [PMID: 11359797] (Flow Cytometry, Human)

Review for CCR1 Antibody (MAB145) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer ICC Other 01/29/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: rabbit spleen primary isolated cells
• Species: Other
• Lot: AXD0414021

Bioinformatics

• Gene Symbol: CCR1
• Uniprot:
  • P32246()