CCR1 Antibody (53504) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
53504
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CCR1 Antibody (53504) [Unconjugated] Summary

Additional Information
Clone 53504 was used by HLDA to establish CD designation
Immunogen
Swiss 3T3 mouse cell line transfected with human CCR1
Accession # P32246
Specificity
Detects human CCR1. Reacts specifically with human CCR1 transfectants and not the parental cell line in flow cytometry.
No cross‑reactivity with CCR5 transfectants is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCR1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 4
using
MAB145 in the following applications:

Publications
Read Publications using
MAB145 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR1 Antibody (53504) [Unconjugated]

  • C-C chemokine receptor type 1
  • CC-CKR-1
  • CCR1
  • CCR-1
  • CD191 antigen
  • CD191
  • chemokine (C-C motif) receptor 1
  • CKR1
  • CKR-1
  • CMKBR1SCYAR1
  • CMKR1
  • HM145C-C CKR-1
  • LD78 receptor
  • Macrophage inflammatory protein 1-alpha receptor
  • MIP-1alpha-R
  • MIP1aR
  • RANTES receptor
  • RANTES-R

Background

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR1 Antibody (MAB145-100)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Flow
(10)
ICC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(2)
Neut
(4)
WB
(1)
All Applications
Filter By Species
Human
(18)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB145 Applications Species
K Vandyke, MN Zeissig, DR Hewett, SK Martin, KM Mrozik, CM Cheong, P Diamond, LB To, S Gronthos, DJ Peet, PI Croucher, ACW Zannettino HIF-2? promotes dissemination of plasma cells in multiple myeloma by regulating CXCL12/CXCR4 and CCR1 Cancer Res., 2017;0(0):. 2017 [PMID: 28855206] (Flow,, Human) Flow Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (ICC,, Human) ICC Human
F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28701512] (Flow,, Human) Flow Human
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. 2016 [PMID: 27496101] (Flow,, Human) Flow Human
Gilliland C, Salanga C, Kawamura T, Trejo J, Handel T The chemokine receptor CCR1 is constitutively active, which leads to G protein-independent, beta-arrestin-mediated internalization. J Biol Chem, 2013;288(45):32194-210. 2013 [PMID: 24056371] (Flow,, Human) Flow Human
Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur. J. Immunol., 2011;41(4):963-73. 2011 [PMID: 21381021] (Neut,, Human) Neut Human
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010;127(2):332-44. 2010 [PMID: 19937793] (Neut,, Human) Neut Human
Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells. Int. J. Cancer, 2010;126(5):1095-108. 2010 [PMID: 19642141] (Neut,, Human) Neut Human
Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009;21(8):991-1001. 2009 [PMID: 19592420] (WB,, Mouse) WB Mouse
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Flow,, Human) Flow Human
Show All 19 Publications.

Review for CCR1 Antibody (MAB145-100) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using MAB145:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CCR1 MAB145
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reviewed by:
Anonymous
ICC Other 01/29/2015
View

Summary

ApplicationImmunocytochemistry
Sample Testedrabbit spleen primary isolated cells
SpeciesOther
LotAXD0414021

Blocking

Blocking Details5% of horse serum in PBS blocking for 1h at RT.

Primary Anitbody

Dilution Ratio1: 25 dilution. Working concentration is 20ug/ml.

Secondary Antibody

Secondary Descriptionbiotinylated horse anti-mouse IgG.
Secondary Manufacturer Cat#Vector. BA-2000
Secondary Concentration1:400 dilution.

Details

Detection NotesDAB as chromogenic agent. develop for 5min at RT. Hematoxylin as counterstain for 1min. Negative control is without primary ab.
Fixation Details4% paraformaldehyde fixed for 30min at RT. Use 10ug/ml proteinase K for antigen retrival at Rt for 20min.
Wash DescriptionWash with 0.01M PBS(PH 7.4) at RT. 3X10min washing after each step.

Comments

CommentsThis mouse anti-human CCR1 antibody crossreact with rabbit spleen primary cell.(please help me delete my review for NBP2-35334 submitted the same day. There is a mistake there, I put the wrong catalog number. Thank you)

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FAQs for CCR1 Antibody (MAB145-100) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on CCR1.

CCR1 (C-C chemokine receptor type 1)
Chemokines play a central role in inflammation and are crucial for recruitment of immune cells to sites of infection. The chemokine-dependent activation of leukocytes occurs through binding to G-protein coupled receptors. These chemokine receptor s...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
01/29/2015
Application: ICC
Species: Other

Bioinformatics

Gene Symbol CCR1
Entrez
Uniprot