Understanding CXCR4 and SDF1

Fri, 07/26/2013 - 08:04

CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand increases intracellular calcium ion levels and enhances activation of the MAPK1/MAPK3 cascade. CXCR4 also acts as a receptor for extracellular ubiquitin, which physiologically results in enhanced intracellular calcium and reduced cellular cAMP levels. CXCR4 is involved in a wide variety of functions ranging from hematopoiesis to cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract by regulating vascular branching and remodeling processes in endothelial cells.

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract. Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.

Researchers used the CXCR4 antibody to profile events occurring during interneuron migration in cerebrocortical development (1). They were able to show that the chemoattractant stromal cell-derived factor-1 (SDF-1) is responsible for regulating the migration and placement of CSCR4-expressing neurons. CXCR4 antibody also allowed the same group in a later study to establish a dual role for SDF-1 in the adult brain in an isoform-specific modulation of SDF-1 transcripts (2). Studies with CXCR4 antibody in malignant peripheral nerve sheath tumors (MPNSTs) found that suppression of CXCR4 via agent inhibition and/or shRNA shut down tumorigenesis and identify a very promising therapeutic target for a highly aggressive and fatal disease (3). Stem cell work using the CXCR4 antibody shows that adrenaline can synergize with granulocyte colony stimulating factor (GSF) to mobilize hematppoietic stem and progenitor cells (HSPC) in a safe and efficient manner without adverse side effects (4). CXCR4 antibody has also been used to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on the homing of circulating osteogenic stem cells to fracture sites in parabiotic mouse models (5).

  1. PMID: 12832536
  2. PMID: 12122049
  3. PMID: 23432321
  4. PMID: 23224606
  5. PMID: 22419401

Novus Biologicals offers various CXCR4 reagents for your research needs including:

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