CXCR3 Antibody (49801) [Unconjugated]

CXCR3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human CXCR3 Monoclonal Antibody (Catalog # MAB160) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A).View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND50 is typically 0.3-1.5 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 49801
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR3 Antibody (49801) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CXCR3
  Met1-Leu368
  Accession # P49682
• Specificity: Detects human CXCR3.
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR3
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR3 Antibody (49801) [Unconjugated]

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR3 Antibody (MAB160)(40)

Publications using MAB160

• F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28701512] (Flow,, Human)
• H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells Oncol Lett, 2017;13(6):4224-4230. 2017 [PMID: 28599423] (WB,, Human)
• N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence Nat Commun, 2017;8(0):15380. 2017 [PMID: 28541275] (Functional Assay,, Human)
• C Windmüller, D Zech, S Avril, M Boxberg, T Dawidek, B Schmalfeld, M Schmitt, M Kiechle, H Bronger CXCR3 mediates ascites-directed tumor cell migration and predicts poor outcome in ovarian cancer patients Oncogenesis, 2017;6(5):e331. 2017 [PMID: 28504691] (WB,, Human)
• JH Han, CH Suh, JY Jung, MH Ahn, MH Han, JE Kwon, H Yim, HA Kim Elevated circulating levels of the interferon-?-induced chemokines are associated with disease activity and cutaneous manifestations in adult-onset Still&#039;s disease Sci Rep, 2017;7(0):46652. 2017 [PMID: 28436448] (IHC,, Human)
• W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia? Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28155222] (Flow,, Human)
• Kuang-Hui Sun TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma J Cell Mol Med, 2016;0(0):. 2016 [PMID: 27297979] (WB,, Human)
• Karjalainen M, Ojaniemi M, Haapalainen A, Mahlman M, Salminen A, Huusko J, Maatta T, Kaukola T, Anttonen J, Ulvila J, Haataja R, Teramo K, Kingsmore S, Palotie A, Muglia L, Ramet M, Hallman M CXCR3 Polymorphism and Expression Associate with Spontaneous Preterm Birth. J Immunol, 2015;195(5):2187-98. 2015 [PMID: 26209629] (WB,, Human)
• McCully M, Collins P, Hughes T, Thomas C, Billen J, O'Donnell V, Moser B Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. J Immunol, 2015;195(1):96-104. 2015 [PMID: 26002980] (Flow,, Human)
• Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res, 2015;21(12):2840-50. 2015 [PMID: 25712684]
• Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T Dysregulation of CXCR3 expression on peripheral blood leukocytes in patients with neovascular age-related macular degeneration. Invest Ophthalmol Vis Sci, 2014;55(7):4050-6. 2014 [PMID: 24812555] (Flow,, Human)
• Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol, 2014;192(8):3805-15. 2014 [PMID: 24646734] (Flow,, Human)
• Bondar C, Araya R, Guzman L, Rua E, Chopita N, Chirdo F Role of CXCR3/CXCL10 axis in immune cell recruitment into the small intestine in celiac disease. PLoS ONE, 2014;9(2):e89068. 2014 [PMID: 24586509] (IF,, Human)
• Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014;14(0):118. 2014 [PMID: 24559071] (ICC Fresh,, Human)
• Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells. J Immunol, 2013;190(10):5321-8. 2013 [PMID: 23576682] (Flow,, Human)
• Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer. Clin Cancer Res, 2013;19(8):2224-31. 2013 [PMID: 23479678] (Flow,, Human)
• Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis. J Clin Invest, 2013;123(1):189-205. 2013 [PMID: 23318994] (WB,, Human)
• Sela H, Goldman-Wohl D, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, Revel A, Lavy Y, Singer O, Yachimovich-Cohen N, Turetsky T, Mandelboim O, Reubinoff B, Yagel S Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation. Placenta, 2013;34(3):222-30. 2013 [PMID: 23306067] (IHC,, Human)
• Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R Ubiquitination of CXCR7 controls receptor trafficking. PLoS ONE, 2012;7(3):e34192. 2012 [PMID: 22457824] (Internalization Assay,, Human)
• Wu Q, Dhir R, Wells A Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion. Mol. Cancer, 2012;11(0):3. 2012 [PMID: 22236567] (WB,, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011;6(10):e26780. 2011 [PMID: 22039549] (Flow,, Human)
• Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009;69(15):6249-55. 2009 [PMID: 19638592] (Flow,, Human)
• Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis. Eur. Respir. J., 2009;34(3):676-86. 2009 [PMID: 19386686] (IHC Paraffin-embedded,, Human)
• Zhu Y, Vergote D, Pardo C, Noorbakhsh F, McArthur JC, Hollenberg MD, Overall CM, Power C CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy. FASEB J., 2009;23(9):2928-41. 2009 [PMID: 19380511] (Functional Assay,, Feline)
• Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. J. Immunol., 2009;182(6):3919-27. 2009 [PMID: 19265171] (IHC Frozen,, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Flow,, Human)
• Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor. J. Immunol., 2008;180(10):6713-24. 2008 [PMID: 18453591] (Flow,, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007;179(6):4272-82. 2007 [PMID: 17785868] (ICC,, Human)
• Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007;179(6):3724-33. 2007 [PMID: 17785809] (Flow,, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neut,, Human)
• Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Chemokine receptor expression in rat adjuvant-induced arthritis. Arthritis Rheum., 2005;52(12):3718-30. 2005 [PMID: 16320322] (IHC,, Rat)
• Srivastava BI, Srivastava MD Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma. Leuk. Res., 2005;29(7):771-83. 2005 [PMID: 15927673] (ICC,, Human)
• Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11. J. Neuroimmunol., 2005;163(1):128-34. 2005 [PMID: 15885315] (Flow,, Human)
• Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus. Arthritis Rheum., 2005;52(5):1504-16. 2005 [PMID: 15880822] (IHC Fresh/Frozen,, Human)
• Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma. Cancer, 2005;103(2):258-67. 2005 [PMID: 15578685] (IHC Paraffin-embedded,, Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004;114(11):1640-9. 2004 [PMID: 15578096] (ICC,, Human)
• van Marle G, Henry S, Todoruk T, Sullivan A, Silva C, Rourke SB, Holden J, McArthur JC, Gill MJ, Power C Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10. Virology, 2004;329(2):302-18. 2004 [PMID: 15518810] (ICC,, Human)
• Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Int. J. Oncol., 2004;25(3):605-13. 2004 [PMID: 15289861] (ICC,, Human)
• Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002;168(12):6244-52. 2002 [PMID: 12055238] (IHC Frozen,, Human)
• de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 2001;31(4):1037-46. 2001 [PMID: 11298328] (Flow,, Human)

Bioinformatics

• Gene Symbol: CXCR3
• Entrez:
  • 2833(Human)
  • 12766(Mouse)
  • 84475(Rat)
• Uniprot:
  • P49682()