Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha

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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Product Details

Summary
Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
Source
E. coli-derived CXCL12/SDF-1 alpha protein
Lys22-Lys89
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL12
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing conditions.
Publications
Read Publications using
350-NS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha . The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

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Publications for CXCL12/SDF-1 alpha (350-NS)(177)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 12 applications: Binding Assay, Bioassay, Cell Culture, Control, ELISA (Standard), ELISA Control, Enzyme Assay, Functional Assay, In Vivo, Migration, Positive Control, Western Blot.


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(2)
Bioassay
(154)
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(4)
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(6)
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(1)
Enzyme Assay
(1)
Functional Assay
(1)
In Vivo
(11)
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(1)
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(1)
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(1)
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Human
(142)
Mouse
(23)
Rat
(5)
Feline
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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All Species
Showing Publications 1 - 10 of 177. Show All 177 Publications.
Publications using 350-NS Applications Species
Lyu, H;Shen, F;Ruan, S;Tan, C;Zhou, J;Thor, AD;Liu, B; HER3 functions as an effective therapeutic target in triple negative breast cancer to potentiate the antitumor activity of gefitinib and paclitaxel Cancer cell international 2023-09-16 [PMID: 37716943] (Bioassay, Human) Bioassay Human
Lim, HJ;Jang, WB;Rethineswaran, VK;Choi, J;Lee, EJ;Park, S;Jeong, Y;Ha, JS;Yun, J;Choi, YJ;Hong, YJ;Kwon, SM; StemRegenin-1 Attenuates Endothelial Progenitor Cell Senescence by Regulating the AhR Pathway-Mediated CYP1A1 and ROS Generation Cells 2023-08-05 [PMID: 37566085] (Bioassay, Human) Bioassay Human
Toffali, L;D'Ulivo, B;Giagulli, C;Montresor, A;Zenaro, E;Delledonne, M;Rossato, M;Iadarola, B;Sbarbati, A;Bernardi, P;Angelini, G;Rossi, B;Lopez, N;Linke, WA;Unger, A;Di Silvestre, D;Benazzi, L;De Palma, A;Motta, S;Constantin, G;Mauri, P;Laudanna, C; An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking Cell reports 2023-05-18 [PMID: 37204926] (Bioassay, Human) Bioassay Human
Shariatzadeh, M;Nagtzaam, NMA;van Vark-van der Zee, L;van Holten-Neelen, C;Verhoeven, AJM;Dehairs, J;Swinnen, JV;Leijten, F;Ten Berge, JC;Ciriano, JPM;Wong, KT;Mulder, M;Leenen, PJM;Dik, WA; Altered Functionality of Lipoprotein(a) Impacts on Angiogenesis in Diabetic Retinopathy Investigative ophthalmology & visual science 2023-05-01 [PMID: 37159194] (Bioassay, Human) Bioassay Human
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer Oncology Letters, 2022-09-30;24(5):417. 2022-09-30 [PMID: 36245829] (Bioassay, Human) Bioassay Human
X Yuan, H Wu, X Li, L Chen, Y Xiao, Z Chen, G Liu, P Lu SDF?1alpha/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathways in�vitro Molecular Medicine Reports, 2022-08-10;26(4):. 2022-08-10 [PMID: 35946444] (Bioassay, Human) Bioassay Human
H Namkoong, R Edahiro, T Takano, H Nishihara, Y Shirai, K Sonehara, H Tanaka, S Azekawa, Y Mikami, H Lee, T Hasegawa, K Okudela, D Okuzaki, D Motooka, M Kanai, T Naito, K Yamamoto, QS Wang, R Saiki, R Ishihara, Y Matsubara, J Hamamoto, H Hayashi, Y Yoshimura, N Tachikawa, E Yanagita, T Hyugaji, E Shimizu, K Katayama, Y Kato, T Morita, K Takahashi, N Harada, T Naito, M Hiki, Y Matsushita, H Takagi, R Aoki, A Nakamura, S Harada, H Sasano, H Kabata, K Masaki, H Kamata, S Ikemura, S Chubachi, S Okamori, H Terai, A Morita, T Asakura, J Sasaki, H Morisaki, Y Uwamino, K Nanki, S Uchida, S Uno, T Nishimura, T Ishiguro, T Isono, S Shibata, Y Matsui, C Hosoda, K Takano, T Nishida, Y Kobayashi, Y Takaku, N Takayanagi, S Ueda, A Tada, M Miyawaki, M Yamamoto, E Yoshida, R Hayashi, T Nagasaka, S Arai, Y Kaneko, K Sasaki, E Tagaya, M Kawana, K Arimura, K Takahashi, T Anzai, S Ito, A Endo, Y Uchimura, Y Miyazaki, T Honda, T Tateishi, S Tohda, N Ichimura, K Sonobe, CT Sassa, J Nakajima, Y Nakano, Y Nakajima, R Anan, R Arai, Y Kurihara, Y Harada, K Nishio, T Ueda, M Azuma, R Saito, T Sado, Y Miyazaki, R Sato, Y Haruta, T Nagasaki, Y Yasui, Y Hasegawa, Y Mutoh, T Kimura, T Sato, R Takei, S Hagimoto, Y Noguchi, Y Yamano, H Sasano, S Ota, Y Nakamori, K Yoshiya, F Saito, T Yoshihara, D Wada, H Iwamura, S Kanayama, S Maruyama, T Yoshiyama, K Ohta, H Kokuto, H Ogata, Y Tanaka, K Arakawa, M Shimoda, T Osawa, H Tateno, I Hase, S Yoshida, S Suzuki, M Kawada, H Horinouchi, F Saito, K Mitamura, M Hagihara, J Ochi, T Uchida, R Baba, D Arai, T Ogura, H Takahashi, S Hagiwara, G Nagao, S Konishi, I Nakachi, K Murakami, M Yamada, H Sugiura, H Sano, S Matsumoto, N Kimura, Y Ono, H Baba, Y Suzuki, S Nakayama, K Masuzawa, S Namba, K Suzuki, Y Naito, YC Liu, A Takuwa, F Sugihara, JB Wing, S Sakakibara, N Hizawa, T Shiroyama, S Miyawaki, Y Kawamura, A Nakayama, H Matsuo, Y Maeda, T Nii, Y Noda, T Niitsu, Y Adachi, T Enomoto, S Amiya, R Hara, Y Yamaguchi, T Murakami, T Kuge, K Matsumoto, Y Yamamoto, M Yamamoto, M Yoneda, T Kishikawa, S Yamada, S Kawabata, N Kijima, M Takagaki, N Sasa, Y Ueno, M Suzuki, N Takemoto, H Eguchi, T Fukusumi, T Imai, M Fukushima, H Kishima, H Inohara, K Tomono, K Kato, M Takahashi, F Matsuda, H Hirata, Y Takeda, H Koh, T Manabe, Y Funatsu, F Ito, T Fukui, K Shinozuka, S Kohashi, M Miyazaki, T Shoko, M Kojima, T Adachi, M Ishikawa, K Takahashi, T Inoue, T Hirano, K Kobayashi, H Takaoka, K Watanabe, N Miyazawa, Y Kimura, R Sado, H Sugimoto, A Kamiya, N Kuwahara, A Fujiwara, T Matsunaga, Y Sato, T Okada, Y Hirai, H Kawashima, A Narita, K Niwa, Y Sekikawa, K Nishi, M Nishitsuji, M Tani, J Suzuki, H Nakatsumi, T Ogura, H Kitamura, E Hagiwara, K Murohashi, H Okabayashi, T Mochimaru, S Nukaga, R Satomi, Y Oyamada, N Mori, T Baba, Y Fukui, M Odate, S Mashimo, Y Makino, K Yagi, M Hashiguchi, J Kagyo, T Shiomi, S Fuke, H Saito, T Tsuchida, S Fujitani, M Takita, D Morikawa, T Yoshida, T Izumo, M Inomata, N Kuse, N Awano, M Tone, A Ito, Y Nakamura, K Hoshino, J Maruyama, H Ishikura, T Takata, T Odani, M Amishima, T Hattori, Y Shichinohe, T Kagaya, T Kita, K Ohta, S Sakagami, K Koshida, K Hayashi, T Shimizu, Y Kozu, H Hiranuma, Y Gon, N Izumi, K Nagata, K Ueda, R Taki, S Hanada, K Kawamura, K Ichikado, K Nishiyama, H Muranaka, K Nakamura, N Hashimoto, K Wakahara, K Sakamoto, N Omote, A Ando, N Kodama, Y Kaneyama, S Maeda, T Kuraki, T Matsumoto, K Yokote, TA Nakada, R Abe, T Oshima, T Shimada, M Harada, T Takahashi, H Ono, T Sakurai, T Shibusawa, Y Kimizuka, A Kawana, T Sano, C Watanabe, R Suematsu, H Sageshima, A Yoshifuji, K Ito, S Takahashi, K Ishioka, M Nakamura, M Masuda, A Wakabayash, H Watanabe, S Ueda, M Nishikawa, Y Chihara, M Takeuchi, K Onoi, J Shinozuka, A Sueyoshi, Y Nagasaki, M Okamoto, S Ishihara, M Shimo, Y Tokunaga, Y Kusaka, T Ohba, S Isogai, A Ogawa, T Inoue, S Fukuyama, Y Eriguchi, A Yonekawa, K Kan-O, K Matsumoto, K Kanaoka, S Ihara, K Komuta, Y Inoue, S Chiba, K Yamagata, Y Hiramatsu, H Kai, K Asano, T Oguma, Y Ito, S Hashimoto, M Yamasaki, Y Kasamatsu, Y Komase, N Hida, T Tsuburai, B Oyama, M Takada, H Kanda, Y Kitagawa, T Fukuta, T Miyake, S Yoshida, S Ogura, S Abe, Y Kono, Y Togashi, H Takoi, R Kikuchi, S Ogawa, T Ogata, S Ishihara, A Kanehiro, S Ozaki, Y Fuchimoto, S Wada, N Fujimoto, K Nishiyama, M Terashima, S Beppu, K Yoshida, O Narumoto, H Nagai, N Ooshima, M Motegi, A Umeda, K Miyagawa, H Shimada, M Endo, Y Ohira, M Watanabe, S Inoue, A Igarashi, M Sato, H Sagara, A Tanaka, S Ohta, T Kimura, Y Shibata, Y Tanino, T Nikaido, H Minemura, Y Sato, Y Yamada, T Hashino, M Shinoki, H Iwagoe, H Takahashi, K Fujii, H Kishi, M Kanai, T Imamura, T Yamashita, M Yatomi, T Maeno, S Hayashi, M Takahashi, M Kuramochi, I Kamimaki, Y Tominaga, T Ishii, M Utsugi, A Ono, T Tanaka, T Kashiwada, K Fujita, Y Saito, M Seike, H Watanabe, H Matsuse, N Kodaka, C Nakano, T Oshio, T Hirouchi, S Makino, M Egi, Biobank Ja, Y Omae, Y Nannya, T Ueno, K Katayama, M Ai, Y Fukui, A Kumanogoh, T Sato, N Hasegawa, K Tokunaga, M Ishii, R Koike, Y Kitagawa, A Kimura, S Imoto, S Miyano, S Ogawa, T Kanai, K Fukunaga, Y Okada DOCK2 is involved in the host genetics and biology of severe COVID-19 Nature, 2022-08-08;609(7928):754-760. 2022-08-08 [PMID: 35940203] (Bioassay, Human) Bioassay Human
S Leng, L Liu, W Xu, F Yang, J Du, L Ye, D Huang, L Zhang Inflammation down regulates stromal cell-derived factor 1alpha in the early phase of pulpitis Cytokine, 2022-08-02;158(0):155983. 2022-08-02 [PMID: 35930964] (Bioassay, Human) Bioassay Human
M Mastrogiov, P Vargas, T Rose, C Cuche, E Esposito, M Juzans, H Laude, A Schneider, M Bernard, S Goyard, C Renaudat, MN Ungeheuer, J Delon, A Alcover, V Di Bartolo The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration Science Advances, 2022-04-13;8(15):eabl5942. 2022-04-13 [PMID: 35417240] (Bioassay, Human) Bioassay Human
Show All 177 Publications.

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Bioinformatics

Gene Symbol CXCL12
Uniprot