Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha

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1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.

Product Details

Summary
Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
Source
E. coli-derived CXCL12/SDF-1 alpha protein
Lys22-Lys89
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL12
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing conditions.
Publications
Read Publications using
350-NS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

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Publications for CXCL12/SDF-1 alpha (350-NS)(144)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), Enzyme Assay, Functional Assay, In Vivo.


Filter By Application
Binding Assay
(2)
Bioassay
(119)
Cell Culture
(2)
ELISA (Standard)
(5)
Enzyme Assay
(1)
Functional Assay
(1)
In Vivo
(12)
All Applications
Filter By Species
Human
(110)
Mouse
(19)
Rat
(4)
Feline
(1)
N/A
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 144. Show All 144 Publications.
Publications using 350-NS Applications Species
H Levy-Baraz, L Shachnai-P, G Rodionov, A Saar, M Rosenzwaig, L Gez, A Rodin, N Marelly, M Abraham, I Mishalian, H Wildbaum, T Katz, Y Baar, S Yarkoni, R Bakimer-Kl, A Peled, T Zuckerman, J Stein Brief ex vivo Fas-ligand incubation attenuates GvHD without compromising stem cell graft performance Bone Marrow Transplant., 2020;0(0):. 2020 [PMID: 32433499] (Bioassay, Mouse) Bioassay Mouse
LA Callender, EC Carroll, EA Bober, AN Akbar, E Solito, SM Henson Mitochondrial mass governs the extent of human T cell senescence Aging Cell, 2019;0(0):e13067. 2019 [PMID: 31788930] (Bioassay, Human) Bioassay Human
PD Fromm, PA Silveira, JL Hsu, MS Papadimitr, TH Lo, X Ju, F Kupresanin, A Romano, WH Hsu, CE Bryant, B Kong, E Abadir, A Mekkawy, H M McGuire, BFS Groth, I Cunningham, E Newman, J Gibson, PM Hogarth, DNJ Hart, GJ Clark Distinguishing human peripheral blood CD16+ myeloid cells based on phenotypic characteristics J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31749181] (Bioassay, Human) Bioassay Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019;20(21):. 2019 [PMID: 31653015] (Bioassay, Human) Bioassay Human
NM Belle, Y Ji, K Herbine, Y Wei, J Park, K Zullo, LY Hung, S Srivatsa, T Young, T Oniskey, C Pastore, W Nieves, M Somsouk, DR Herbert TFF3 interacts with LINGO2 to regulate EGFR activation for protection against colitis and gastrointestinal helminths Nat Commun, 2019;10(1):4408. 2019 [PMID: 31562318] (Bioassay, Human) Bioassay Human
J Yang, L Zhang, Z Jiang, C Ge, F Zhao, J Jiang, H Tian, T Chen, H Xie, Y Cui, M Yao, H Li, J Li TCF12 promotes the tumorigenesis and metastasis of hepatocellular carcinoma via upregulation of CXCR4 expression Theranostics, 2019;9(20):5810-5827. 2019 [PMID: 31534521] (Bioassay, Human) Bioassay Human
L Chen, J Ouyang, K Wienand, K Bojarczuk, Y Hao, B Chapuy, D Neuberg, P Juszczynsk, LN Lawton, SJ Rodig, S Monti, MA Shipp CXCR4 upregulation is an indicator of sensitivity to B-cell receptor/PI3K blockade and a potential resistance mechanism in B-cell receptor-dependent diffuse large B-cell lymphomas Haematologica, 2020;105(5):1361-1368. 2020 [PMID: 31471373] (Functional Assay, Human) Functional Assay Human
C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019;10(8):562. 2019 [PMID: 31332163] (In Vivo, Mouse) In Vivo Mouse
ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019;130(0):2555-2570. 2019 [PMID: 31107242] (Bioassay, Mouse) Bioassay Mouse
W Liu, X Wang, S Wang, X Ba, T Xu, X Wang, X Zeng RhoGDI2 positively regulates the Rho GTPases activation in response to the ?2 outside-in signaling in T cells adhesion and migration on ICAM-1 J. Leukoc. Biol., 2019;0(0):. 2019 [PMID: 31075185] (Bioassay, Human) Bioassay Human
Show All 144 Publications.

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Bioinformatics

Gene Symbol CXCL12
Uniprot