CCR7 Antibody (150503) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # ...read more
CCR7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) for 3 hours at room temperature. Cells were stained ...read more
CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation ...read more
Recombinant Human CCL19/MIP‑3 beta (Catalog # 361‑MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated ...read more
HCMV strain-dependent variability of UL4015-23 sequences and HCMV strain-specific HLA-EUL40 T-cell response in hosts.(A, B) Genomic DNAs isolated from HCMV positive blood samples in HCMV+ transplant recipients (n = 25) ...read more
CXCR4 ligand binding facilitates CXCR4/CCR7 hetero-oligomer formation.(A) CCR7 homo-oligomer formation in H9 cells after treatment with or without 1 μg/ml recombinant gp120 was examined by in situ PLA. The number of in ...read more
Dynamics of CCR7 expression on CD1c+ mDC and pDC during primary SIVmac239 infection.(A) The dynamics of CCR7 expression on CD1c+ mDC; (B) The dynamics of CCR7 expression on pDC. Image collected and cropped by CiteAb ...read more
CCR7 homodimers are dissociated by the CCR7 TM4-derived peptide. (A) The levels of bioluminescence signals are presented for HEK293T cells transfected with the indicated combinations of CCR7-CGLuc, CCR7-NGLuc, CGLuc and ...read more
Two-color strategy for identifying CD1c+ mDCs and pDCs.(A) PBMCs (R1) were first gated on Forward-scatter/side-scatter (FSC/SSC) scattergram. Then, CD1c+ mDCs (CD1c+CD14−CD20−) were selected in the R2 region and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready, ICC/IF
Clone
150503
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR7 Antibody (150503) [Unconjugated] Summary

Additional Information
Clone 150503 was used by HLDA to establish CD designation
Immunogen
Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615
Specificity
Detects human CCR7.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CCR7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB197 in the following applications:

Publications
Read Publications using
MAB197 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR7 Antibody (150503) [Unconjugated]

  • BLR2
  • BLR2C-C chemokine receptor type 7
  • C-C CKR-7
  • CC-CKR-7
  • CCR7
  • CCR-7
  • CD197 antigen
  • CD197
  • CDw197
  • CDw197CC chemokine receptor 7
  • chemokine (C-C motif) receptor 7
  • CMKBR7
  • CMKBR7chemokine (C-C) receptor 7
  • EBI1
  • EBI1EBV-induced G protein-coupled receptor 1
  • EBV-induced G-protein coupled receptor 1
  • Epstein-Barr virus induced gene 1
  • Epstein-Barr virus induced G-protein coupled receptor
  • Epstein-Barr virus-induced G-protein coupled receptor 1
  • EVI1
  • lymphocyte-specific G protein-coupled peptide receptor
  • MIP-3 beta receptor

Background

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR7 Antibody (MAB197)(88)

We have publications tested in 9 confirmed species: Human, Mouse, Canine, Complex Species Category, Marmoset, Primate, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 10 applications: Blocking, CyTof, Flow Cytometry, Flow Cytometry (CAR-T), Functional Assay, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Blocking
(2)
CyTof
(4)
Flow Cytometry
(62)
Flow Cytometry (CAR-T)
(1)
Functional Assay
(1)
ICC
(6)
IHC
(5)
IHC-P
(1)
Neutralization
(7)
Western Blot
(3)
All Applications
Filter By Species
Human
(70)
Mouse
(1)
Canine
(1)
Complex Species Category
(1)
Marmoset
(1)
Primate
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(11)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 88. Show All 88 Publications.
Publications using MAB197 Applications Species
M Oba, Y Nakanishi, T Mitsuhashi, K Sasaki, KC Hatanaka, M Sasaki, A Nange, A Okumura, M Hayashi, Y Yoshida, T Nitta, T Ueno, T Yamada, M Ono, S Kuwabara, K Okamura, T Tsuchikawa, T Nakamura, T Noji, T Asano, K Tanaka, K Takayama, Y Hatanaka, S Hirano CCR7 Mediates Cell Invasion and Migration in Extrahepatic Cholangiocarcinoma by Inducing Epithelial-Mesenchymal Transition Cancers, 2023-03-21;15(6):. 2023-03-21 [PMID: 36980764] (Neutralization, Human) Neutralization Human
SA Rahman, J Billinglsl, AA Sharma, TM Styles, S Govindaraj, U Shanmugasu, H Babu, SP Ribeiro, SA Ali, GK Tharp, C Ibegbu, S Waggoner, RP Johnson, RP Sékaly, F Villinger, SE Bosinger, RR Amara, V Velu Lymph node CXCR5+ NK cells associate with control of chronic SHIV infection JCI Insight, 2022-04-22;0(0):. 2022-04-22 [PMID: 35271506] (Flow Cytometry, Human) Flow Cytometry Human
AA Okoye, R Fromentin, H Takata, JH Brehm, Y Fukazawa, B Randall, M Pardons, V Tai, J Tang, J Smedley, M Axthelm, JD Lifson, LJ Picker, D Favre, L Trautmann, N Chomont The ingenol-based protein kinase C agonist GSK445A is a potent inducer of HIV and SIV RNA transcription PloS Pathogens, 2022-01-18;18(1):e1010245. 2022-01-18 [PMID: 35041707] (ICC, Human) ICC Human
I Del Molino, A Meeson, K Cooke, MI Malki, B Barron-Mil, JA Kirby, S Ali Contribution of Heparan Sulphate Binding in CCL21-Mediated Migration of Breast Cancer Cells Cancers, 2021-07-10;13(14):. 2021-07-10 [PMID: 34298676] (ICC, IHC, Human) ICC, IHC Human
FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&amp;alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Neutralization, Human) Neutralization Human
A Jerram, TV Guy, L Beutler, B Gunasegara, R Sluyter, B Fazekas de, HM McGuire Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Bioscience Reports, 2021-02-26;41(2):. 2021-02-26 [PMID: 33600563] (CyTof, Human) CyTof Human
JA van Ipenbu, NE de Waard, NC Naus, MJ Jager, D Paridaens, RM Verdijk Chemokine Receptor Expression Pattern Correlates to Progression of Conjunctival Melanocytic Lesions Invest. Ophthalmol. Vis. Sci., 2019-07-01;60(8):2950-2957. 2019-07-01 [PMID: 31305861] (IHC-P, Human) IHC-P Human
Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment Cancer Immunol Res, 2019-04-15;0(0):. 2019-04-15 [PMID: 30988027] (Flow Cytometry, Human) Flow Cytometry Human
AA Okoye, SG Hansen, M Vaidya, Y Fukazawa, H Park, DM Duell, R Lum, CM Hughes, AB Ventura, E Ainslie, JC Ford, D Morrow, RM Gilbride, AW Legasse, J Hesselgess, R Geleziunas, Y Li, K Oswald, R Shoemaker, R Fast, WJ Bosche, BR Borate, PT Edlefsen, MK Axthelm, LJ Picker, JD Lifson Early antiretroviral therapy limits SIV reservoir establishment to delay or prevent post-treatment viral rebound Nat. Med., 2018-08-06;0(0):. 2018-08-06 [PMID: 30082858] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque)) Flow Cytometry Primate - Macaca mulatta (Rhesus Macaque)
T Alexander, Q Cheng, J Klotsche, L Khodadadi, A Waka, R Biesen, BF Hoyer, GR Burmester, A Radbruch, F Hiepe Proteasome inhibition with bortezomib induces a therapeutically relevant depletion of plasma cells in SLE but does not target their precursors Eur. J. Immunol., 2018-07-30;0(0):. 2018-07-30 [PMID: 29979809] (Flow Cytometry, Human) Flow Cytometry Human
Show All 88 Publications.

Review for CCR7 Antibody (MAB197) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB197:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Human 02/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23731727
SpeciesHuman

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Verified Customer
02/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol CCR7
Uniprot