CCR7 Antibody (150503) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # ...read more
CCR7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) for 3 hours at room temperature. Cells were stained ...read more
CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation ...read more
Recombinant Human CCL19/MIP‑3 beta (Catalog # 361‑MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-reported, ICC/IF
Clone
150503
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CCR7 Antibody (150503) [Unconjugated] Summary

Additional Information
Clone 150503 was used by HLDA to establish CD designation
Immunogen
Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615
Specificity
Detects human CCR7.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CCR7
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB197 in the following applications:

Publications
Read Publications using
MAB197 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR7 Antibody (150503) [Unconjugated]

  • BLR2C-C chemokine receptor type 7
  • C-C CKR-7
  • CC-CKR-7
  • CCR7
  • CCR-7
  • CD197 antigen
  • CD197
  • CDw197CC chemokine receptor 7
  • chemokine (C-C motif) receptor 7
  • CMKBR7chemokine (C-C) receptor 7
  • EBI1EBV-induced G protein-coupled receptor 1
  • EBV-induced G-protein coupled receptor 1
  • Epstein-Barr virus induced gene 1
  • Epstein-Barr virus induced G-protein coupled receptor
  • Epstein-Barr virus-induced G-protein coupled receptor 1
  • EVI1
  • lymphocyte-specific G protein-coupled peptide receptor
  • MIP-3 beta receptor

Background

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR7 Antibody (MAB197)(76)

We have publications tested in 10 confirmed species: Human, Mouse, Canine, Complex, Marmoset, Primate, Primate - Cercocebus torquatus (Sooty Mangabey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 11 applications: Blocking, CyTof, Flow, Functional Assay, ICC, ICC Fresh, IHC, IHC Not Specified, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Blocking
(1)
CyTof
(3)
Flow
(58)
Functional Assay
(1)
ICC
(2)
ICC Fresh
(1)
IHC
(1)
IHC Not Specified
(1)
IHC Paraffin-embedded
(1)
Neut
(4)
WB
(3)
All Applications
Filter By Species
Human
(58)
Mouse
(1)
Canine
(1)
Complex
(1)
Marmoset
(1)
Primate
(1)
Primate - Cercocebus torquatus (Sooty Mangabey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(10)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using MAB197 Applications Species
T Alexander, Q Cheng, J Klotsche, L Khodadadi, A Waka, R Biesen, BF Hoyer, GR Burmester, A Radbruch, F Hiepe Proteasome inhibition with bortezomib induces a therapeutically relevant depletion of plasma cells in SLE but does not target their precursors Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29979809] (Flow,, Human) Flow Human
N Jouand, C Bressollet, N Gérard, M Giral, P Guérif, A Rodallec, R Oger, T Parrot, M Allard, A Cesbron-Ga, N Gervois, B Charreau HCMV triggers frequent and persistent UL40-specific unconventional HLA-E-restricted CD8 T-cell responses with potential autologous and allogeneic peptide recognition PLoS Pathog., 2018;14(4):e1007041. 2018 [PMID: 29709038] (Flow,, Human) Flow Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (ICC,, Human) ICC Human
M Mata, C Gerken, P Nguyen, G Krenciute, DM Spencer, S Gottschalk Inducible activation of MyD88 and CD40 in CAR T-cells results in controllable and potent antitumor activity in preclinical solid tumor models Cancer Discov, 2017;0(0):. 2017 [PMID: 28801306] (Flow,, Human) Flow Human
JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017;2017(0):1975902. 2017 [PMID: 28785596] (Flow,, Human) Flow Human
F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28701512] (Flow,, Human) Flow Human
L Huang, NHR Litjens, NM Kannegiete, M Klepper, CC Baan, MGH Betjes pERK-dependent defective TCR-mediated activation of CD4(+) T cells in end-stage renal disease patients Immun Ageing, 2017;14(0):14. 2017 [PMID: 28642802] (Flow,, Human) Flow Human
SM Nguyen, KM Antony, DM Dudley, S Kohn, HA Simmons, B Wolfe, MS Salamat, LBC Teixeira, GJ Wiepz, TH Thoong, MT Aliota, AM Weiler, GL Barry, KL Weisgrau, LJ Vosler, MS Mohns, ME Breitbach, LM Stewart, MN Rasheed, CM Newman, ME Graham, OE Wieben, PA Turski, KM Johnson, J Post, JM Hayes, N Schultz-Da, ML Schotzko, JA Eudailey, SR Permar, EG Rakasz, EL Mohr, S Capuano, AF Tarantal, JE Osorio, SL O'Connor, TC Friedrich, DH O'Connor, TG Golos Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques PLoS Pathog., 2017;13(5):e1006378. 2017 [PMID: 28542585] (Flow,, Primate - Macaca mulatta (Rhesus Macaque)) Flow Primate - Macaca mulatta (Rhesus Macaque)
Z Zhang, F Liu, Z Li, D Wang, R Li, C Sun Jak3 is involved in CCR7-dependent migration and invasion in metastatic squamous cell carcinoma of the head and neck Oncol Lett, 2017;13(5):3191-3197. 2017 [PMID: 28521425] (Neut,, Human) Neut Human
FG Karnell, D Lin, S Motley, T Duhen, N Lim, DJ Campbell, LA Turka, HT Maecker, KM Harris Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation Clin. Exp. Immunol., 2017;0(0):. 2017 [PMID: 28498568] (CyTof,, Human) CyTof Human
Show All 76 Publications.

Review for CCR7 Antibody (MAB197) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB197:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 02/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23731727
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23731727

Details

Detection NotesSee PMID 23731727

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23731727

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Recent Reviews

5
5
1
4
0
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1
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Anonymous
02/12/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol CCR7
Entrez
Uniprot