CCR7 Antibody (150503) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CCR7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CCR7 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Recombinant Human CCL19/MIP‑3 beta (Catalog # 361‑MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197). The ND50 is typically 1-5 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-reported, ICC/IF
• Clone: 150503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR7 Antibody (150503) [Unconjugated] Summary

• Additional Information: Clone 150503 was used by HLDA to establish CD designation
• Immunogen: Human CCR7 transfectants
  Met1-Pro378
  Accession # AAA58615
• Specificity: Detects human CCR7.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization 1-5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR7 Antibody (150503) [Unconjugated]

• BLR2
• BLR2C-C chemokine receptor type 7
• C-C CKR-7
• CC-CKR-7
• CCR7
• CCR-7
• CD197 antigen
• CD197
• CDw197
• CDw197CC chemokine receptor 7
• chemokine (C-C motif) receptor 7
• CMKBR7
• CMKBR7chemokine (C-C) receptor 7
• EBI1
• EBI1EBV-induced G protein-coupled receptor 1
• EBV-induced G-protein coupled receptor 1
• Epstein-Barr virus induced gene 1
• Epstein-Barr virus induced G-protein coupled receptor
• Epstein-Barr virus-induced G-protein coupled receptor 1
• EVI1
• lymphocyte-specific G protein-coupled peptide receptor
• MIP-3 beta receptor

Background

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR7 Antibody (MAB197)(84)

Publications using MAB197

• SA Rahman, J Billinglsl, AA Sharma, TM Styles, S Govindaraj, U Shanmugasu, H Babu, SP Ribeiro, SA Ali, GK Tharp, C Ibegbu, S Waggoner, RP Johnson, RP Sékaly, F Villinger, SE Bosinger, RR Amara, V Velu Lymph node CXCR5+ NK cells associate with control of chronic SHIV infection JCI Insight, 2022;0(0):. 2022 [PMID: 35271506] (Flow Cytometry, Human)
• AA Okoye, R Fromentin, H Takata, JH Brehm, Y Fukazawa, B Randall, M Pardons, V Tai, J Tang, J Smedley, M Axthelm, JD Lifson, LJ Picker, D Favre, L Trautmann, N Chomont The ingenol-based protein kinase C agonist GSK445A is a potent inducer of HIV and SIV RNA transcription PloS Pathogens, 2022;18(1):e1010245. 2022 [PMID: 35041707] (ICC, Human)
• I Del Molino, A Meeson, K Cooke, MI Malki, B Barron-Mil, JA Kirby, S Ali Contribution of Heparan Sulphate Binding in CCL21-Mediated Migration of Breast Cancer Cells Cancers, 2021;13(14):. 2021 [PMID: 34298676] (ICC, Human)
• FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&amp;alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 33676046] (Neutralization, Human)
• A Jerram, TV Guy, L Beutler, B Gunasegara, R Sluyter, B Fazekas de, HM McGuire Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Bioscience Reports, 2021;41(2):. 2021 [PMID: 33600563] (CyTof, Human)
• JA van Ipenbu, NE de Waard, NC Naus, MJ Jager, D Paridaens, RM Verdijk Chemokine Receptor Expression Pattern Correlates to Progression of Conjunctival Melanocytic Lesions Invest. Ophthalmol. Vis. Sci., 2019;60(8):2950-2957. 2019 [PMID: 31305861] (IHC-P, Human)
• Q Haas, K Frias Boli, C Jandus, C Schneider, C Simillion, MA Stanczak, M Haubitz, SM Seyed Jafa, A Zippelius, GM Baerlocher, H Läubli, RE Hunger, P Romero, HU Simon, S von Gunten Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 30988027] (Flow Cytometry, Human)
• AA Okoye, SG Hansen, M Vaidya, Y Fukazawa, H Park, DM Duell, R Lum, CM Hughes, AB Ventura, E Ainslie, JC Ford, D Morrow, RM Gilbride, AW Legasse, J Hesselgess, R Geleziunas, Y Li, K Oswald, R Shoemaker, R Fast, WJ Bosche, BR Borate, PT Edlefsen, MK Axthelm, LJ Picker, JD Lifson Early antiretroviral therapy limits SIV reservoir establishment to delay or prevent post-treatment viral rebound Nat. Med., 2018;0(0):. 2018 [PMID: 30082858] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• T Alexander, Q Cheng, J Klotsche, L Khodadadi, A Waka, R Biesen, BF Hoyer, GR Burmester, A Radbruch, F Hiepe Proteasome inhibition with bortezomib induces a therapeutically relevant depletion of plasma cells in SLE but does not target their precursors Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29979809] (Flow Cytometry, Human)
• N Jouand, C Bressollet, N Gérard, M Giral, P Guérif, A Rodallec, R Oger, T Parrot, M Allard, A Cesbron-Ga, N Gervois, B Charreau HCMV triggers frequent and persistent UL40-specific unconventional HLA-E-restricted CD8 T-cell responses with potential autologous and allogeneic peptide recognition PLoS Pathog., 2018;14(4):e1007041. 2018 [PMID: 29709038] (Flow Cytometry, Human)
• D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (ICC, Human)
• M Mata, C Gerken, P Nguyen, G Krenciute, DM Spencer, S Gottschalk Inducible activation of MyD88 and CD40 in CAR T-cells results in controllable and potent antitumor activity in preclinical solid tumor models Cancer Discov, 2017;0(0):. 2017 [PMID: 28801306] (Flow Cytometry, Human)
• JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use J Immunol Res, 2017;2017(0):1975902. 2017 [PMID: 28785596] (Flow Cytometry, Human)
• F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28701512] (Flow Cytometry, Human)
• L Huang, NHR Litjens, NM Kannegiete, M Klepper, CC Baan, MGH Betjes pERK-dependent defective TCR-mediated activation of CD4(+) T cells in end-stage renal disease patients Immun Ageing, 2017;14(0):14. 2017 [PMID: 28642802] (Flow Cytometry, Human)
• SM Nguyen, KM Antony, DM Dudley, S Kohn, HA Simmons, B Wolfe, MS Salamat, LBC Teixeira, GJ Wiepz, TH Thoong, MT Aliota, AM Weiler, GL Barry, KL Weisgrau, LJ Vosler, MS Mohns, ME Breitbach, LM Stewart, MN Rasheed, CM Newman, ME Graham, OE Wieben, PA Turski, KM Johnson, J Post, JM Hayes, N Schultz-Da, ML Schotzko, JA Eudailey, SR Permar, EG Rakasz, EL Mohr, S Capuano, AF Tarantal, JE Osorio, SL O'Connor, TC Friedrich, DH O'Connor, TG Golos Highly efficient maternal-fetal Zika virus transmission in pregnant rhesus macaques PLoS Pathog., 2017;13(5):e1006378. 2017 [PMID: 28542585] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Z Zhang, F Liu, Z Li, D Wang, R Li, C Sun Jak3 is involved in CCR7-dependent migration and invasion in metastatic squamous cell carcinoma of the head and neck Oncol Lett, 2017;13(5):3191-3197. 2017 [PMID: 28521425] (Neutralization, Human)
• FG Karnell, D Lin, S Motley, T Duhen, N Lim, DJ Campbell, LA Turka, HT Maecker, KM Harris Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation Clin. Exp. Immunol., 2017;0(0):. 2017 [PMID: 28498568] (CyTof, Human)
• J Dunham, N van Driel, BJ Eggen, C Paul, BA 't Hart, JD Laman, YS Kap Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset Clin Transl Immunology, 2017;6(2):e127. 2017 [PMID: 28243437] (Flow Cytometry, Primate)
• T Koya, R Yanagisawa, Y Higuchi, K Sano, S Shimodaira Interferon-?-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity Sci Rep, 2017;7(0):42145. 2017 [PMID: 28191816] (Flow Cytometry, Human)
• S Pichyangku, MD Spring, K Yongvanitc, U Kum-Arb, A Limsalakpe, R Im-Erbsin, R Ubalee, P Vanachayan, EJ Remarque, E Angov, PL Smith, DL Saunders Chemoprophylaxis with sporozoite immunization in P. knowlesi rhesus monkeys confers protection and elicits sporozoite-specific memory T cells in the liver PLoS ONE, 2017;12(2):e0171826. 2017 [PMID: 28182750] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Fas/CD95 prevents autoimmunity independently of lipid raft localization and efficient apoptosis induction Nat Commun, 2016;7(0):13895. 2016 [PMID: 28008916] (Flow Cytometry, Human)
• Vassilios Lougaris NFKB1 regulates human NK cell maturation and effector functions Clin. Immunol, 2016;0(0):. 2016 [PMID: 27923702] (Flow Cytometry, Human)
• Evan W Newell Optimization of mass cytometry sample cryopreservation after staining Cytometry A, 2016;0(0):. 2016 [PMID: 27798817] (CyTof, Human)
• Compartmentalization of Total and Virus-Specific Tissue-Resident Memory CD8+ T Cells in Human Lymphoid Organs PLoS Pathog, 2016;12(8):e1005799. 2016 [PMID: 27540722] (Flow Cytometry, Human)
• Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016;6(0):24530. 2016 [PMID: 27456172] (Flow Cytometry, Human)
• Heightened Immune Activation in Fetuses with Gastroschisis May Be Blocked by Targeting IL-5 J Immunol, 2016;0(0):. 2016 [PMID: 27183609] (Flow Cytometry, Human)
• CF Kelley, L Lai, C Ibegbu, ES Rosenberg, S Kaur, K Patel, MJ Mulligan, VC Marconi, PS Sullivan, RR Amara Differences in expression of gut-homing receptors on CD4+ T cells in black and white HIV-negative men who have sex with men AIDS, 2016;30(8):1305-8. 2016 [PMID: 26891038] (Flow Cytometry, Human)
• Okoye A, Rohankhedkar M, Konfe A, Abana C, Reyes M, Clock J, Duell D, Sylwester A, Sammader P, Legasse A, Park B, Axthelm M, Nikolich-Zugich J, Picker L Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques. J Immunol, 2015;195(9):4292-305. 2015 [PMID: 26416281] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Pichyangkul S, Yongvanitchit K, Limsalakpetch A, Kum-Arb U, Im-Erbsin R, Boonnak K, Thitithayanont A, Jongkaewwattana A, Wiboon-ut S, Mongkolsirichaikul D, Mahanonda R, Spring M, Chuang I, Mason C, Saunders D Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection. J Immunol, 2015;195(9):4378-86. 2015 [PMID: 26408671] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• White M, Gomer R Trypsin, Tryptase, and Thrombin Polarize Macrophages towards a Pro-Fibrotic M2a Phenotype. PLoS ONE, 2015;10(9):e0138748. 2015 [PMID: 26407067] (IHC, Human)
• Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Cancer Res, 2015;75(19):4153-63. 2015 [PMID: 26282174] (Flow Cytometry, Human)
• Mei H, Leipold M, Schulz A, Chester C, Maecker H Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. J Immunol, 2015;194(4):2022-31. 2015 [PMID: 25609839] (CyTof, Human)
• Takakura K, Koido S, Kan S, Yoshida K, Mori M, Hirano Y, Ito Z, Kobayashi H, Takami S, Matsumoto Y, Kajihara M, Misawa T, Okamoto M, Sugiyama H, Homma S, Ohkusa T, Tajiri H Prognostic markers for patient outcome following vaccination with multiple MHC Class I/II-restricted WT1 peptide-pulsed dendritic cells plus chemotherapy for pancreatic cancer. Anticancer Res, 2015;35(1):555-62. 2015 [PMID: 25550602] (Flow Cytometry, Human)
• Williams G, Pachnio A, Long H, Rauz S, Curnow S Cytokine production and antigen recognition by human mucosal homing conjunctival effector memory CD8+ T cells. Invest Ophthalmol Vis Sci, 2014;55(12):8523-30. 2014 [PMID: 25395484] (Flow Cytometry, Human)
• Xuan W, Qu Q, Zheng B, Xiong S, Fan G The chemotaxis of M1 and M2 macrophages is regulated by different chemokines. J Leukoc Biol, 2015;97(1):61-9. 2015 [PMID: 25359998] (Neutralization, Human)
• Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression. Placenta, 2014;35(12):1089-94. 2014 [PMID: 25293376] (Western Blot, Human)
• Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nat Commun, 2014;5(0):4715. 2014 [PMID: 25178650] (Blocking, Human)
• Antsiferova O, Muller A, Ramer P, Chijioke O, Chatterjee B, Raykova A, Planas R, Sospedra M, Shumilov A, Tsai M, Delecluse H, Munz C Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. PLoS Pathog, 2014;10(8):e1004333. 2014 [PMID: 25165855] (Flow Cytometry, Human)
• Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE, 2014;9(8):e106008. 2014 [PMID: 25162594] (Flow Cytometry, Human)
• Campion S, Brodie T, Fischer W, Korber B, Rossetti A, Goonetilleke N, McMichael A, Sallusto F Proteome-wide analysis of HIV-specific naive and memory CD4(+) T cells in unexposed blood donors. J Exp Med, 2014;211(7):1273-80. 2014 [PMID: 24958850] (Flow Cytometry, Human)
• Borgman K, van Zanten T, Manzo C, Cabezon R, Cambi A, Benitez-Ribas D, Garcia-Parajo M Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells. PLoS ONE, 2014;9(6):e99589. 2014 [PMID: 24945611] (Flow Cytometry, Human)
• Xu Y, Zhang M, Ramos C, Durett A, Liu E, Dakhova O, Liu H, Creighton C, Gee A, Heslop H, Rooney C, Savoldo B, Dotti G Closely related T-memory stem cells correlate with in vivo expansion of CAR.CD19-T cells and are preserved by IL-7 and IL-15. Blood, 2014;123(24):3750-9. 2014 [PMID: 24782509] (Flow Cytometry (CAR-T), Human)
• Kap , Yolanda, van Driel , Nikki, Laman , Jon D, Tak , Paul P, 't Hart , Bert A CD20+ B cell depletion alters T cell homing. J Immunol, 2014;192(9):4242-53. 2014 [PMID: 24696233] (Flow Cytometry, Marmoset)
• Carrega , Paolo, Bonaccorsi , Irene, Di Carlo , Emma, Morandi , Barbara, Paul , Petra, Rizzello , Valeria, Cipollone , Giuseppe, Navarra , Giuseppe, Mingari , Maria Cr, Moretta , Lorenzo, Ferlazzo , Guido CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph. J Immunol, 2014;192(8):3805-15. 2014 [PMID: 24646734] (Flow Cytometry, Human)
• Rongvaux , Anthony, Willinger , Tim, Martinek , Jan, Strowig , Till, Gearty , Sofia V, Teichmann , Lino L, Saito , Yasuyuki, Marches , Florenti, Halene , Stephani, Palucka , A Karoli, Manz , Markus G, Flavell , Richard Development and function of human innate immune cells in a humanized mouse model. Nat Biotechnol, 2014;32(4):364-72. 2014 [PMID: 24633240] (Flow Cytometry, Mouse)
• Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014;14(0):118. 2014 [PMID: 24559071] (ICC, Human)
• Ishikawa , Takeshi, Kokura , Satoshi, Enoki , Tatsuji, Sakamoto , Naoyuki, Okayama , Tetsuya, Ideno , Mitsuko, Mineno , Junichi, Uno , Kazuko, Yoshida , Naohisa, Kamada , Kazuhiro, Katada , Kazuhiro, Uchiyama , Kazuhiko, Handa , Osamu, Takagi , Tomohisa, Konishi , Hideyuki, Yagi , Nobuaki, Naito , Yuji, Itoh , Yoshito, Yoshikawa , Toshikaz Phase I clinical trial of fibronectin CH296-stimulated T cell therapy in patients with advanced cancer. PLoS ONE, 2014;9(1):e83786. 2014 [PMID: 24497917] (Flow Cytometry, Human)
• Somovilla-Crespo B, Alfonso-Perez M, Cuesta-Mateos C, Carballo-de Dios C, Beltran A, Terron F, Perez-Villar J, Gamallo-Amat C, Perez-Chacon G, Fernandez-Ruiz E, Zapata J, Munoz-Calleja C Anti-CCR7 therapy exerts a potent anti-tumor activity in a xenograft model of human mantle cell lymphoma. J Hematol Oncol, 2013;6(0):89. 2013 [PMID: 24305507] (Neutralization, Human)
• Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells. J Immunol, 2013;190(10):5321-8. 2013 [PMID: 23576682] (Flow Cytometry, Human)
• Manes , Thomas D, Pober , Jordan S TCR-driven transendothelial migration of human effector memory CD4 T cells involves Vav, Rac, and myosin IIA. J Immunol, 2013;190(7):3079-88. 2013 [PMID: 23420881] (Functional Assay, Human)
• Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013;131(4):1146-56, 1156. 2013 [PMID: 23403044] (Flow Cytometry, Human)
• Santegoets S, Turksma A, Suhoski M, Stam A, Albelda S, Hooijberg E, Scheper R, van den Eertwegh A, Gerritsen W, Powell D, June C, de Gruijl T IL-21 promotes the expansion of CD27+ CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells. J Transl Med, 2015;11(0):37. 2015 [PMID: 23402380] (Flow Cytometry, Human)
• Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation. Eur J Immunol, 2013;43(2):550-61. 2013 [PMID: 23192659] (Flow Cytometry, Human)
• Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2013;6(3):626-38. 2013 [PMID: 23131784] (Flow Cytometry, Human)
• Accelerated heterologous adenovirus prime-boost SIV vaccine in neonatal rhesus monkeys. Oncogene, 2012;86(15):7829-35. 2012 [PMID: 22593160] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012;484(7395):514-8. 2012 [PMID: 22466287] (Flow Cytometry, Human)
• Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA Identification of human NK17/NK1 cells. PLoS ONE, 2011;6(10):e26780. 2011 [PMID: 22039549] (Flow Cytometry, Human)
• Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection. J. Immunol., 2011;186(9):5151-61. 2011 [PMID: 21441446] (Flow Cytometry, Primate - Cercocebus torquatus (Sooty Mangabey))
• Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011;12(2):167-77. 2011 [PMID: 21217759] (Flow Cytometry, Human)
• Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, Villinger F Blocking of alpha4beta7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques. J. Immunol., 2011;186(2):1044-59. 2011 [PMID: 21149598] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection. J. Immunol., 2010;184(1):315-26. 2010 [PMID: 19949091] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, O'Connor D, Lifson JD, Roederer M, Ohlen C Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. J. Immunol., 2010;184(1):303-14. 2010 [PMID: 19949089] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets. Blood, 2010;115(2):274-81. 2010 [PMID: 19897577] (Flow Cytometry, Human)
• Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia. Ann. Neurol., 2009;66(4):521-31. 2009 [PMID: 19847900] (Flow Cytometry, Human)
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Review for CCR7 Antibody (MAB197) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: cynthia morimoto IF Human 02/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23731727
• Species: Human

Bioinformatics

• Gene Symbol: CCR7
• Uniprot:
  • AAA58615()