CCR3 Antibody (61828)

Images

 
CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat ...read more
Recombinant Human CCL11/Eotaxin (Catalog # 320‑EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
61828
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR3 Antibody (61828) Summary

Additional Information
Clone 61828 was used by HLDA to establish CD designation
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCR3
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.05-0.25 ug/mL
Publications
Read Publications using
MAB155 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR3 Antibody (61828)

  • b-chemokine receptor
  • CC chemokine receptor 3
  • C-C chemokine receptor type 3
  • C-C CKR-3
  • CCR3
  • CCR-3
  • CD193 antigen
  • CD193
  • chemokine (C-C motif) receptor 3
  • CKR3CC-CKR-3CMKBR3CD193
  • eosinophil CC chemokine receptor 3
  • Eosinophil eotaxin receptor
  • MGC102841

Background

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR3 Antibody (MAB155)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Flow Cytometry
(8)
ICC
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(3)
Neutralization
(7)
All Applications
Filter By Species
Human
(18)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using MAB155 Applications Species
Y Kaibori, D Nagakubo CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines Cancer Gene Therapy, 2022-09-19;0(0):. 2022-09-19 [PMID: 36123391] (ICC, Human) ICC Human
MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells MBio, 2021-08-17;12(4):e0196221. 2021-08-17 [PMID: 34399621] (Neutralization, Human) Neutralization Human
Michal Segal-Salto, Neta Barashi, Avi Katav, Vicktoria Edelshtein, Arnon Aharon, Sharon Hashmueli et al. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage JHEP Reports 2020-02-01 [PMID: 32039405]
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016-08-05;0(0):. 2016-08-05 [PMID: 27496101] (Flow Cytometry, Human) Flow Cytometry Human
Guichun Huang Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells Sci Rep, 2016-06-02;6(0):27152. 2016-06-02 [PMID: 27250766] (Neutralization, Human) Neutralization Human
Expression and prognostic significance of CCL11/CCR3 in glioblastoma Oncotarget, 2016-05-31;7(22):32617-27. 2016-05-31 [PMID: 27119233] (IHC-P, Human) IHC-P Human
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (Neutralization, Mouse) Neutralization Mouse
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010-07-15;127(2):332-44. 2010-07-15 [PMID: 19937793] (Neutralization, Human) Neutralization Human
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, ICC, IHC-P, Neutralization, Human) Flow Cytometry, ICC, IHC-P, Neutralization Human
Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-03-29;25(7):1737-45. 2007-03-29 [PMID: 17395768] (Flow Cytometry, Human) Flow Cytometry Human
Show All 20 Publications.

Reviews for CCR3 Antibody (MAB155) (0)

There are no reviews for CCR3 Antibody (MAB155). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCR3 Antibody (MAB155) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCR3 Antibody (61828) and receive a gift card or discount.

Bioinformatics

Gene Symbol CCR3
Uniprot