CCR3 Antibody (61828)

CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human CCL11/Eotaxin (Catalog # 320‑EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 61828
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR3 Antibody (61828) Summary

• Additional Information: Clone 61828 was used by HLDA to establish CD designation
• Immunogen: Y3 rat myeloid cell line transfected with human CCR3
  Accession # P51677
• Specificity: Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR3
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.05-0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR3 Antibody (61828)

• b-chemokine receptor
• CC chemokine receptor 3
• C-C chemokine receptor type 3
• C-C CKR-3
• CCR3
• CCR-3
• CD193 antigen
• CD193
• chemokine (C-C motif) receptor 3
• CKR3CC-CKR-3CMKBR3CD193
• eosinophil CC chemokine receptor 3
• Eosinophil eotaxin receptor
• MGC102841

Background

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR3 Antibody (MAB155)(19)

Publications using MAB155

• Y Kaibori, D Nagakubo CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines Cancer Gene Therapy, 2022;0(0):. 2022 [PMID: 36123391] (ICC, Human)
• MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells MBio, 2021;12(4):e0196221. 2021 [PMID: 34399621] (Neutralization, Human)
• Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. 2016 [PMID: 27496101] (Flow Cytometry, Human)
• Guichun Huang Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells Sci Rep, 2016;6(0):27152. 2016 [PMID: 27250766] (Neutralization, Human)
• Expression and prognostic significance of CCL11/CCR3 in glioblastoma Oncotarget, 2016;7(22):32617-27. 2016 [PMID: 27119233] (IHC-P, Human)
• Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011;475(7355):226-30. 2011 [PMID: 21753853] (Neutralization, Mouse)
• Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010;127(2):332-44. 2010 [PMID: 19937793] (Neutralization, Human)
• Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Flow Cytometry, Human)
• Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007;25(7):1737-45. 2007 [PMID: 17395768] (Flow Cytometry, Human)
• Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells. Eur. J. Immunol., 2006;36(10):2700-14. 2006 [PMID: 16983721] (Neutralization, Human)
• Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C Functional analysis of the chemokine receptor CCR3 on airway epithelial cells. J. Immunol., 2006;177(5):3344-54. 2006 [PMID: 16920975] (IHC-P, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neutralization, Human)
• Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion. Proc. Natl. Acad. Sci. U.S.A., 2006;103(9):3333-8. 2006 [PMID: 16492782] (Flow Cytometry, Human)
• Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts. J. Allergy Clin. Immunol., 2005;117(1):103-10. 2005 [PMID: 16387592] (IHC, Human)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004;173(11):6592-602. 2004 [PMID: 15557149] (Flow Cytometry, Human)
• Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003;103(7):2806-8. 2003 [PMID: 14656893] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003;73(5):584-90. 2003 [PMID: 12714572] (Flow Cytometry, Human)
• Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. J. Exp. Med., 2002;196(6):781-91. 2002 [PMID: 12235211] (IHC-Fr, Human)
• de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 2001;31(4):1037-46. 2001 [PMID: 11298328] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR3
• Uniprot:
  • P51677()