Collagen I alpha 1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen 1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry: Collagen I Antibody [NBP1-77457] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Bovine, Canine, Equine, Primate, SheepSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Concentration
1.1 mg/ml

Collagen I alpha 1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Collagen I protein (between residues 150-200) [UniProt P02452]
Localization
Secreted to extracellular space and extracellular matrix.
Predicted Species
Primate (100%), Equine (90%), Bovine (94%), Canine (94%), Sheep (94%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
COL1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Collagen 1 antibody is useful for IHC-paraffin embedded sections.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen's sequence similarity with other species: Rat (77%), Chicken (71%), Rabbit (69%).

Alternate Names for Collagen I alpha 1 Antibody

  • Alpha-1 type I collagen
  • COL1A1
  • Collagen 1 alpha 1
  • Collagen 1
  • collagen alpha 1 chain type I
  • collagen alpha-1(I) chain
  • Collagen I alpha 1
  • collagen of skin, tendon and bone, alpha-1 chain
  • collagen, type I, alpha 1
  • Collagen1
  • Collagen-1
  • OI4
  • pro-alpha-1 collagen type 1

Background

Collagen-1 (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues, and synthesizes by fibroblasts, osteoblasts, and odontoblasts. Trimers are formed by combing two alpha one chains and one alpha 2 chain. Mutations of the gene are found in Ehlers-Danlos syndrome, as well as osteogenesis imperfecta. Over 50 mutations have been found in these diseases, including deletions, insertions, splice variants, and substitutions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Collagen I alpha 1 Antibody (NBP1-77457): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Collagen I alpha 1 Antibody (NBP1-77457). (Showing 1 - 9 of 9 FAQ).

  1. We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?
    • We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).
  2. I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?
    • Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).
  3. Can this collagen 1 alpha 1 antibody be conjugated?
    • Unfortunately we do not offer this particular collagen 1 alpha 1 antibody in a directly conjugated form; however you may want to consider one of our other collagen I alpha 1 antibodies such as NBP1-77458 that is offered in several conjugated forms.
  4. What is the epitope for this collagen I alpha 1 antibody? I need to make sure that there won’t be cross reactivity with other collagens.
    • This collagen I alpha 1 antibody has not been epitope mapped; however, it has been shown there is less than 1% cross reactivity between other collagen forms and this collagen I alpha 1 antibody.
  5. What is the immunogen for this collagen antibody?
    • Pepsin digested type 1 collagen from human and bovine placenta was used as the immunogen for this collagen I alpha 1 antibody.
  6. The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?
    • Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.
  7. How long does delivery take for your collagen 1 alpha 1 antibody?
    • Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.
  8. What secondary antibody do you recommend for collagen I alpha 1 antibodies?
    • Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.
  9. Can you tell me if this antibody will react with macrophages? What blocking solution was used in the protocol for collagen I?
    • According to UniProt, Collagen I forms the fibrils of tendon, ligaments and bones. However, there is evidence of Collagen I being produced in macrophages, (abstract, full article). For IHC, we recommend using 5% animal serum in PBS-T. The animal serum should be of the host the secondary antibody was generated in.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77457AF405
Alexa Fluor 488 Labeled NBP1-77457AF488
Alexa Fluor 647 Labeled NBP1-77457AF647
Alexa Fluor 700 Labeled NBP1-77457AF700
Biotin Labeled NBP1-77457B
DyLight 405 Labeled NBP1-77457V
DyLight 488 Labeled NBP1-77457G
DyLight 550 Labeled NBP1-77457R
DyLight 650 Labeled NBP1-77457C
FITC Labeled NBP1-77457F

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol COL1A1
Entrez
Uniprot

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