CD31/PECAM-1 Antibody

Images

 
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB100-2284] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CD31/PECAM (NB100-2284) used at a dilution of 1:100. Detection: ...read more
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB100-2284] - FFPE section of human ovarian carcinoma. Used at a dilution of 1:250.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB100-2284] - Human lung adenocarcinoma. Used at a dilution of 1:100 with citrate epitope retrieval pH6.0.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody [NB100-2284] - Analysis in pig SC cells using anti-CD31 antibody (red). Blue color showing nucleus labeling. Image from verified customer review.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody [NB100-2284] - Anaysis in rat muscle blood vessels using anti-CD31 antibody. Image from verified customer review.
Immunohistochemistry: CD31/PECAM-1 Antibody [NB100-2284] - Section of human colon adenocarcinoma.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD31/PECAM-1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 738) of human CD31 using the numbering given in entry NP_000433.2 (GeneID 5175).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PECAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
For IHC-P: Epitope exposure is recommended, with citrate buffer will enhance staining. In some cases, the antibody may be diluted further than indicated. Immunohistochemistry-Frozen and Western Blot were reported in scientific literature. ICC/IF reactivity reported in scientific literature (PMID: 27328066).
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-2284 in the following applications:

Publications
Read Publications using
NB100-2284 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23317813)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD31/PECAM-1 Antibody

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells and is specifically concentrated at the junction between them. Therefore, CD31 antibodies are commonly used as endothelial markers to measure angiogenesis in association with tumor recurrence. It is also weakly expressed on many peripheral lymphoid cells and platelets. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). It is also weakly expressed on many peripheral lymphoid cells and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2284
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD31/PECAM-1 Antibody (NB100-2284)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
IHC-Fr
(2)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-2284 Applications Species
Mohamed R, Sharma I, Ibrahim AS et al. Hyperhomocysteinemia Alters Retinal Endothelial Cells Barrier Function and Angiogenic Potential via Activation of Oxidative Stress Sci Rep 2017 Sep 20 [PMID: 28931831] (ICC/IF, Human) ICC/IF Human
Ehret C, Aid-Launais R, Sagardoy T et al. Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation PLoS ONE 2017 Sep 14 [PMID: 28910401] (IHC-P, Human) IHC-P Human
Meyers MW, Rink JS, Jiang Q et al. Systemically administered collagen-targeted gold nanoparticles bind to arterial injury following vascular interventions. Physiol Rep. 2017 Feb 01 [PMID: 28242820] (ICC/IF) ICC/IF
Oliveira H, Catros S, Castano O et al. The proangiogenic potential of a novel calcium releasing composite biomaterial: orthotopic in vivo evaluation. Acta Biomater. 2017 Feb 24 [PMID: 28242456] (WB, Rat) WB Rat
Luo L, Chen B, Huang Y et al. Cardioprotective activity of placental growth factor combined with oral supplementation of l-arginine in a rat model of acute myocardial infarction. Drug Des Devel Ther. Nov 8 2016 [PMID: 27822012] (Rat) Rat
Moon J, Kim Jh, Choi D et al. Correlation of quantitative dynamic contrast-enhanced MRI with microvascular density in necrotic, partial necrotic, and viable liver tumors in a rabbit model. J Appl Clin Med Phys Sep 30 2016 [PMID: 27685133] (IHC) IHC
Modulevsky DJ, Cuerrier CM, Pelling AE. Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials. PLoS One 2016 Jun 21 [PMID: 27328066] (ICC/IF) ICC/IF
De Paoli F, Eeckhoute J, Copin C et al. The neuron-derived orphan receptor 1 (NOR1) is induced upon human alternative macrophage polarization and stimulates the expression of markers of the M2 phenotype Atherosclerosis 2015 [PMID: 25941992] (IHC-Fr, Human) IHC-Fr Human
Bories G, Colin S, Vanhoutte J et al. Liver X Receptor (LXR) Activation Stimulates Iron Export in Human Alternative Macrophages. Circ Res. 2013 Sep 13 [PMID: 24036496] (IHC, Human) IHC Human
Hylander BL, Punt N, Tang H et al. Origin of the vasculature supporting growth of primary patient tumor xenografts. J Transl Med 2013 May 3 [PMID: 23639003] (IHC-P, Human) IHC-P Human
Show All 18 Publications.

Reviews for CD31/PECAM-1 Antibody (NB100-2284) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Rat, Other.
We have 3 reviews tested in 2 application: ICC, IHC-P.

Reviews using NB100-2284:
Filter by Applications
ICC
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CD31/PECAM-1 NB100-2284
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reviewed by:
Fabio Marongiu
ICC Human 08/29/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHUVEC (Human Umbilical Vein Endothelial Cells)
SpeciesHuman
Lot2

Comments

CommentsFixation 4% PFA
Blocking Goat Serum
Dilution 1:100
Incubation 2h @ RT
Immunocytochemistry CD31/PECAM-1 NB100-2284
Enlarge
reviewed by:
Anonymous
ICC Other 01/07/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedSC cells
SpeciesOther

Blocking

Blocking Details-

Secondary Antibody

Secondary DescriptionAlexa 594

Details

Detection Notes-
Immunohistochemistry-Paraffin CD31/PECAM-1 NB100-2284
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reviewed by:
Naiche Adler
IHC-P Rat 01/27/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrat muscle FFPE
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NB100-2284). (Showing 1 - 3 of 3 FAQs).

  1. I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?
    • NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a SS at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.
  2. I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?
    • As is listed on our datasheet, NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a "SS" at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.
  3. I am looking for references concerning the use of NBP2-15797, NBP1-47913 and NB100-2284 in IHC on PFA- fixed section.
    • Among your mentioned catalog numbers (NBP2-15797, NBP1-47913 and NB100-2284), we have published references for NB100-2284 only. Hylander et al. 2013 (Journal of Translational Medicine 2013, 11:110) have cited the IHC-P use of NB100-2284 and this paper has also explained a nice detailed IHC-P protocol that you may follow if you want to. NBP1-71663 is our bestselling catalog number among all CD31/PECAM1 antibodies and it has been cited in two publications, both explaining its use in IHC-P assays (Pratheeshkumar et al. 2012 PLoS One. 2012;7(10):e47516; and Pratheeshkumar et al. 2012 PLoS One. 2012;7(12):e52279)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD31/PECAM-1 Antibody (NB100-2284)

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Diseases for CD31/PECAM-1 Antibody (NB100-2284)

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Fabio Marongiu
08/29/2017
Application: ICC
Species: Human

Anonymous
01/07/2016
Application: ICC
Species: Other

Naiche Adler
01/27/2015
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot